BuphA.12800.s

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.s
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.s.A1.GE32406 Full length( BuphA.12800.s ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.6907
RefSeq: YP_001862644.1
UniProt: B2JSP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKSPNLDDL RVFCTVARQA SFSAAAEALS VSAAYVSKRV AMLEADLGTR LFHRSTRRVA ITDAGERVYA WAEKILDDVD RLVEDVSTTR RVPRGMLRVS SSFGFGRHVV APALARLTER YPQLSVRLDL FDRIVDVAGE GFDLDIRIGD EIAAHLIAKR LASNHRVLCA APAYLKRHGT PRQLADLGAH QCLAIKERDH PSGVWRLTER GETVSVKVTG PLSTNHGEVA VQWALAGRGI VLRSIWDVRG LLDSGALVQV LPGVTQPANV WAVYPARLAS SAKVRVCVDF LADEFGRLAE PGGPQ
NT Sequence
GTGAATAAAT CGCCGAACCT TGACGACTTG CGTGTGTTCT GCACCGTCGC CCGCCAAGCC AGCTTCAGCG CGGCGGCGGA GGCGCTGTCG GTGTCGGCGG CGTATGTGAG CAAGCGCGTG GCCATGCTCG AAGCCGATCT CGGCACGCGG CTTTTTCACC GCTCCACGCG CCGCGTCGCG ATCACGGACG CGGGCGAGCG CGTCTACGCG TGGGCCGAAA AGATTCTCGA CGACGTCGAC CGTCTCGTTG AGGACGTGTC GACCACGCGA CGCGTGCCGC GGGGCATGTT GCGCGTATCG AGCAGTTTCG GTTTCGGCCG GCATGTCGTG GCGCCCGCGC TGGCACGGCT CACGGAGCGC TATCCGCAAC TGAGCGTGCG GCTCGATCTG TTCGATCGCA TCGTCGACGT CGCGGGCGAA GGCTTCGACC TCGATATCCG CATCGGCGAC GAGATTGCTG CGCATCTGAT CGCGAAACGC CTCGCGTCGA ATCATCGCGT GCTGTGCGCG GCGCCAGCTT ATCTGAAGCG GCACGGCACG CCGCGTCAGC TGGCGGATCT CGGCGCGCAT CAATGCCTTG CGATCAAGGA GCGCGACCAT CCGTCCGGCG TCTGGCGTCT GACGGAACGC GGCGAAACCG TGTCGGTGAA GGTAACCGGG CCGCTGTCGA CCAACCACGG CGAAGTGGCC GTGCAATGGG CGCTGGCAGG GCGCGGCATC GTGCTGCGTT CGATCTGGGA TGTGCGCGGG CTGCTGGACA GCGGCGCACT GGTGCAGGTG CTGCCGGGCG TCACGCAGCC TGCGAACGTG TGGGCCGTGT ATCCGGCGCG GCTCGCTTCG TCAGCGAAGG TGCGCGTGTG CGTCGACTTT CTGGCCGACG AGTTCGGCCG TCTGGCCGAA CCCGGCGGGC CTCAG
Details for BuphA.12800.s.A1.GE32406
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVNKSPNLD DLRVFCTVAR QASFSAAAEA LSVSAAYVSK RVAMLEADLG TRLFHRSTRR VAITDAGERV YAWAEKILDD VDRLVEDVST TRRVPRGMLR VSSSFGFGRH VVAPALARLT ERYPQLSVRL DLFDRIVDVA GEGFDLDIRI GDEIAAHLIA KRLASNHRVL CAAPAYLKRH GTPRQLADLG AHQCLAIKER DHPSGVWRLT ERGETVSVKV TGPLSTNHGE VAVXWALAXR GIVLRSIWDV RGLLDSGALV XVLPGVTQPA NVWAVXPXRL ASSAKXRVCV DFLXDEFGRL XEXXGXQ
Validated NT Sequence
ttttgtttnn ctttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggtga ataaatcgcc gaaccttgac gacttgcgtg tgttctgcac cgtcgcccgc caagccagct tcagcgcggc ggcggaggcg ctgtcggtgt cggcggcgta tgtgagcaag cgcgtggcca tgctcgaagc cgatctcggc acgcggcttt ttcaccgctc cacgcgccgc gtcgcgatca cggacgcggg cgagcgcgtc tacgcgtggg ccgaaaagat tctcgacgac gtcgaccgtc tcgttgagga cgtgtcgacc acgcgacgcg tgccgcgggg catgttgcgc gtatcgagca gtttcggttt cggccggcat gtcgtggcgc ccgcgctggc acggctcacg gagcgctatc cgcaactgag cgtgcggctc gatctgttcg atcgcatcgt cgacgtcgcg ggcgaaggct tcgacctcga tatccgcatc ggcgacgaga ttgctgcgca tctgatcgcg aaacgcctcg cgtcgaatca tcgcgtgctg tgcgcggcgc cagcttatct gaagcggcac ggcacgccgc gtcagctggc ggatctcggc gcgcatcaat gccttgcgat caaggagcgc gaccatccgt ccggcgtctg gcgtctgacg gaacgcggcg aaaccgtgtc ggtgaaggta accgggccgc tgtcgaccaa ccacggcgaa gtggccgtgc natgggcgct ggcanggcgc ggcatcgtgc tgcgttcgat ctgggatgtg cgcgggctgc tggacagcgg cgcactggtg cangtgctgc cgggcgtcac gcagcctgcg aacgtgtggg ccgtgtntcc ngngcggctc gcttcgtcag cgaaggngcg cgtgtgcgtc gactttctgg ncgacgagtt cggccgtctg gncgaanccn gcggnnnnca gtaataaaca gcacganang tnctgcnnnc nncttcncna ggnnnnnctg ctannaannc ngaaaggaan ctgantngnt gntgcnnnnn nnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVNKSPNLD DLRVFCTVAR QASFSAAAEA LSVSAAYVSK RVAMLEADLG TRLFHRSTRR VAITDAGERV YAWAEKILDD VDRLVEDVST TRRVPRGMLR VSSSFGFGRH VVAPALARLT ERYPQLSVRL DLFDRIVDVA GEGFDLDIRI GDEIAAHLIA KRLASNHRVL CAAPAYLKRH GTPRQLADLG AHQCLAIKER DHPSGVWRLT ERGETVSVKV TGPLSTNHGE VAVQWALAGR GIVLRSIWDV RGLLDSGALV QVLPGVTQPA NVWAVYPARL ASSAKVRVCV DFLADEFGRL AEPGGPQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgaataaa tcgccgaacc ttgacgactt gcgtgtgttc tgcaccgtcg cccgccaagc cagcttcagc gcggcggcgg aggcgctgtc ggtgtcggcg gcgtatgtga gcaagcgcgt ggccatgctc gaagccgatc tcggcacgcg gctttttcac cgctccacgc gccgcgtcgc gatcacggac gcgggcgagc gcgtctacgc gtgggccgaa aagattctcg acgacgtcga ccgtctcgtt gaggacgtgt cgaccacgcg acgcgtgccg cggggcatgt tgcgcgtatc gagcagtttc ggtttcggcc ggcatgtcgt ggcgcccgcg ctggcacggc tcacggagcg ctatccgcaa ctgagcgtgc ggctcgatct gttcgatcgc atcgtcgacg tcgcgggcga aggcttcgac ctcgatatcc gcatcggcga cgagattgct gcgcatctga tcgcgaaacg cctcgcgtcg aatcatcgcg tgctgtgcgc ggcgccagct tatctgaagc ggcacggcac gccgcgtcag ctggcggatc tcggcgcgca tcaatgcctt gcgatcaagg agcgcgacca tccgtccggc gtctggcgtc tgacggaacg cggcgaaacc gtgtcggtga aggtaaccgg gccgctgtcg accaaccacg gcgaagtggc cgtgcaatgg gcgctggcag ggcgcggcat cgtgctgcgt tcgatctggg atgtgcgcgg gctgctggac agcggcgcac tggtgcaggt gctgccgggc gtcacgcagc ctgcgaacgt gtgggccgtg tatccggcgc ggctcgcttc gtcagcgaag gtgcgcgtgt gcgtcgactt tctggccgac gagttcggcc gtctggccga acccggcggg cctcagtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa