SSGCID: Target Page

BuphA.12800.r

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.r
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.r.A1.GE32405	Full length( BuphA.12800.r )		1	308

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.6869
RefSeq:	YP_001862610.1
UniProt:	B2JSL1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRIDAMKVF VATLDEGSLA GASRRLGRSP AAVSRAIAFL EAHVGSPLLH RTTRTIKLSE AGERYAAACR RILTDLEEAD LLVAGERSAP RGLLNVTAPV AAGQDLLRSL LDDFIDAYPA VSVRLHMLDR PVSLIDEGID VALRIAHLPD STLVAIPVGE VRRVVTAAPR YLAKHPRIHE PGDLAEHQII SMTHFGLDSW SFPPAEGSTV PQVVTFSPRL IVNSIDAAVA SAVEGRGVTR VFSYHVAQQV KDGHLRIVLR DSEHAPLPVH LLTPHGRLSV PKVRAFVDFA TPRLRRQFKQ LQRATDRD

NT Sequence

ATGGACCGGA TTGACGCAAT GAAGGTGTTC GTCGCGACGC TGGACGAAGG CAGCCTGGCG GGCGCGAGCC GCCGGCTCGG CCGTTCGCCT GCCGCGGTGA GCCGCGCCAT TGCGTTTCTC GAAGCGCACG TCGGCTCGCC GCTTCTACAT CGCACCACGC GTACCATCAA ACTGAGCGAA GCGGGCGAAC GTTATGCGGC CGCGTGCCGG CGCATCCTCA CCGATCTCGA GGAGGCCGAC CTGCTGGTAG CGGGTGAACG CTCCGCGCCG CGCGGCCTGC TGAACGTCAC AGCCCCCGTC GCTGCCGGAC AGGACTTGCT GCGCTCGCTC CTCGACGATT TCATCGACGC GTACCCAGCC GTGTCGGTAC GCTTGCACAT GCTCGACCGG CCCGTGAGTC TCATCGACGA AGGGATCGAC GTTGCGCTGC GCATCGCGCA TCTGCCCGAT TCCACGCTCG TGGCAATTCC CGTCGGCGAA GTGCGGCGCG TCGTGACAGC CGCGCCGCGC TACCTGGCGA AGCATCCACG CATCCATGAG CCCGGGGACC TCGCGGAACA TCAGATCATT TCGATGACGC ACTTCGGCCT CGACTCATGG AGCTTTCCAC CGGCGGAAGG CTCGACCGTG CCGCAAGTCG TCACCTTCTC GCCGCGCCTG ATCGTCAACA GCATCGACGC GGCTGTCGCT TCCGCCGTCG AAGGGCGCGG CGTCACGCGC GTGTTTTCGT ATCACGTCGC GCAACAGGTG AAGGACGGGC ACTTGCGGAT CGTGCTGCGC GACAGCGAGC ACGCGCCCTT GCCCGTGCAT CTTCTGACGC CGCACGGCCG GCTTTCGGTG CCGAAAGTGC GCGCGTTCGT CGATTTCGCC ACGCCGCGCC TGCGCCGGCA ATTCAAACAG CTCCAGCGCG CGACAGACCG CGAT

Details for BuphA.12800.r.A1.GE32405

HARVESTED ON:	11/8/2011
SEQUENCED ON:	11/11/2011
EXPECTED MW:	35kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDRIDAMKV FVATLDEGSL AGASRRLGRS PAAVSRAIAF LEAHVGSPLL HRTTRTIKLS EAGERYAAAC RRILTDLEEA DLLVAGERSA PRGLLNVTAP VAAGQDLLRS LLDDFIDAYP AVSVRLHMLD RPVSLIDEGI DVALRIAHLP DSTLVAIPVG EVRRVVTAAP RYLAKHPRIH EPGDLAEHQI ISMTHFGLDS WSFPPAEGST VPQVVTFSPR LIVNSIDAAV ASAVEGRGVT RVFSYHVAQQ VKDGHLRIVL RDSEHAPLPV HLLTPHGRLS VPKVRAFVDF ATPRLRRQFK QLQRATDRD

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccggatt gacgcaatga aggtgttcgt cgcgacgctg gacgaaggca gcctggcggg cgcgagccgc cggctcggcc gttcgcctgc cgcggtgagc cgcgccattg cgtttctcga agcgcacgtc ggctcgccgc ttctacatcg caccacgcgt accatcaaac tgagcgaagc gggcgaacgt tatgcggccg cgtgccggcg catcctcacc gatctcgagg aggccgacct gctggtagcg ggtgaacgct ccgcgccgcg cggcctgctg aacgtcacag cccccgtcgc tgccggacag gacttgctgc gctcgctcct cgacgatttc atcgacgcgt acccagccgt gtcggtacgc ttgcacatgc tcgaccggcc cgtgagtctc atcgacgaag ggatcgacgt tgcgctgcgc atcgcgcatc tgcccgattc cacgctcgtg gcaattcccg tcggcgaagt gcggcgcgtc gtgacagccg cgccgcgcta cctggcgaag catccacgca tccatgagcc cggggacctc gcggaacatc agatcatttc gatgacgcac ttcggcctcg actcatggag ctttccaccg gcggaaggct cgaccgtgcc gcaagtcgtc accttctcgc cgcgcctgat cgtcaacagc atcgacgcgg ctgtcgcttc cgccgtcgaa gggcgcggcg tcacgcgcgt gttttcgtat cacgtcgcgc aacaggtgaa ggacgggcac ttgcggatcg tgctgcgcga cagcgagcac gcgcccttgc ccgtgcatct tctgacgccg cacggccggc tttcggtgcc gaaagtgcgc gcgttcgtcg atttcgccac gccgcgcctg cgccggcaat tcaaacagct ccagcgcgcg acagaccgcg attaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa