BuphA.12800.q

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.q
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.q.A1.GE32404 Full length( BuphA.12800.q ) 1 319
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3513
RefSeq: YP_001859528.1
UniProt: B2JSA5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MIDRIQAMRT FMRIVDTNSF TRAAESLEIP RATATTIVQN LEALLGTTLL VRTTRRLSLT PEGAAYYERC AQILADIDEM EATLRQTLDN PSGRLRVEMP GAVATSIVLP ALDDFHERYP SIDLAIGVSH RNVDLVGEAV DCSIQLGELP DSGLAAKRLG TLEHVTCASP AYLERYGMPE SLDDLSEHVA VNCMSALTGR AVDFDFDVGG SPLTVKVNGF IKVSDEHAYL TCGLQGLGLI QPARIAAQAL LDKGLLREVL PQWKPVPMPV SVAYVKGRRV APRVRAFVDW LAELFEHQGH VEADLSRVRM LLQGRLHAV
NT Sequence
GTGATCGATC GTATTCAGGC GATGCGCACG TTCATGCGTA TTGTCGATAC CAACAGCTTC ACCCGCGCGG CGGAGTCGCT GGAAATTCCG CGCGCGACGG CAACCACCAT TGTGCAGAAC CTGGAAGCGC TGCTTGGCAC GACGCTGCTC GTGCGCACGA CGCGGCGTCT GAGCCTCACG CCGGAAGGCG CGGCCTATTA CGAGCGCTGT GCGCAGATCC TCGCGGATAT CGACGAGATG GAAGCGACAC TGCGCCAGAC GCTCGACAAT CCGAGCGGAC GTCTGCGAGT CGAAATGCCG GGTGCTGTTG CGACTTCAAT CGTGCTGCCC GCGCTCGATG ATTTTCACGA GCGCTATCCG TCGATCGATC TCGCGATCGG CGTCAGTCAT CGCAACGTCG ATCTCGTCGG CGAAGCGGTG GATTGCAGTA TCCAGCTCGG AGAGCTGCCC GATTCGGGCC TGGCTGCCAA GCGGCTCGGG ACGCTCGAGC ACGTGACGTG TGCGAGTCCT GCGTATCTGG AGCGTTACGG CATGCCGGAG AGTCTCGACG ATCTGTCGGA GCATGTGGCC GTGAACTGCA TGTCGGCGCT GACGGGACGC GCCGTCGATT TCGATTTCGA TGTCGGCGGC AGTCCGTTGA CCGTGAAGGT GAACGGCTTC ATCAAGGTCA GCGACGAGCA TGCGTATCTC ACCTGCGGGT TGCAGGGGCT TGGATTGATC CAGCCTGCGA GGATCGCTGC GCAAGCGTTG CTTGATAAGG GCCTGTTGCG TGAGGTCCTG CCGCAGTGGA AGCCGGTGCC GATGCCCGTT TCCGTTGCTT ATGTGAAGGG GCGGCGTGTC GCGCCGCGGG TCAGAGCTTT TGTCGATTGG CTCGCTGAGT TGTTTGAGCA CCAGGGGCAC GTCGAGGCGG ATTTGTCCCG GGTCAGGATG TTGCTGCAGG GTCGCTTGCA TGCGGTT
Details for BuphA.12800.q.A1.GE32404
HARVESTED ON: 11/8/2011
SEQUENCED ON: 11/11/2011
EXPECTED MW: 37kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MVIDRIQAMR TFMRIVDTNS FTRAAESLEI PRATATTIVQ NLEALLGTTL LVRTTRRLSL TPEGAAYYER CAQILADIDE MEATLRQTLD NPSGRLRVEM PGAVATSIVL PALDDFHERY PSIDLAIGVS HRNVDLVGEA VDCSIQLGEL PDSGLAAKRL GTLEHVTCAS PAYLERYGMP ESLDDLSEHV AVNCMSALTG RAVDFDFDVG GSPLTVKVNG FIKVSDEHAY LTCGLQGLGL IQPARIAAQA LLDKGLLREV LPQWKPVPMP VSVAYVKGRR VAPRVRAFVD WLAELFEHQG HVEADLSRVR MLLQGRLHAV
Validated NT Sequence
aaaccgcatg caagcgaccc tgcagcaaca tcctgacccg ggacaaatcc gcctcgacgt gcccctggtg ctcaaacaac tcagcgagcc aatcgacaaa agctctgacc cgcggcgcga cacgccgccc cttcacataa gcaacggaaa cgggcatcgg caccggcttc cactgcggca ggacctcacg caacaggccc ttatcaagca acgcttgcgc agcgatcctc gcaggctgga tcaatccaag cccctgcaac ccgcaggtga gatacgcatg ctcgtcgctg accttgatga agccgttcac cttcacggtc aacggactgc cgccgacatc gaaatcgaaa tcgacggcgc gtcccgtcag cgccgacatg cagttcacgg ccacatgctc cgacagatcg tcgagactct ccggcatgcc gtaacgctcc agatacgcag gactcgcaca cgtcacgtgc tcgagcgtcc cgagccgctt ggcagccagg cccgaatcgg gcagctctcc gagctggata ctgcaatcca ccgcttcgcc gacgagatcg acgttgcgat gactgacgcc gatcgcgaga tcgatcgacg gatagcgctc gtgaaaatca tcgagcgcgg gcagcacgat tgaagtcgca acagcacccg gcatttcgac tcgcagacgt ccgctcggat tgtcgagcgt ctggcgcagt gtcgcttcca tctcgtcgat atccgcgagg atctgcgcac agcgctcgta ataggccgcg ccttccggcg tgaggctcag acgccgcgtc gtgcgcacga gcagcgtcgt gccaagcagc gcttccaggt tctgcacaat ggtggttgcc gtcgcgcgcg gaatttccag cgactccgcc gcgcgggtga agctgttggt atcgacaata cgcatgaacg tgcgcatcgc ctgaatacga tcgatcacca tcgaaccagg accctgggtc tgagcttcca gggtaccca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVIDRIQAM RTFMRIVDTN SFTRAAESLE IPRATATTIV QNLEALLGTT LLVRTTRRLS LTPEGAAYYE RCAQILADID EMEATLRQTL DNPSGRLRVE MPGAVATSIV LPALDDFHER YPSIDLAIGV SHRNVDLVGE AVDCSIQLGE LPDSGLAAKR LGTLEHVTCA SPAYLERYGM PESLDDLSEH VAVNCMSALT GRAVDFDFDV GGSPLTVKVN GFIKVSDEHA YLTCGLQGLG LIQPARIAAQ ALLDKGLLRE VLPQWKPVPM PVSVAYVKGR RVAPRVRAFV DWLAELFEHQ GHVEADLSRV RMLLQGRLHA V
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgatcgat cgtattcagg cgatgcgcac gttcatgcgt attgtcgata ccaacagctt cacccgcgcg gcggagtcgc tggaaattcc gcgcgcgacg gcaaccacca ttgtgcagaa cctggaagcg ctgcttggca cgacgctgct cgtgcgcacg acgcggcgtc tgagcctcac gccggaaggc gcggcctatt acgagcgctg tgcgcagatc ctcgcggata tcgacgagat ggaagcgaca ctgcgccaga cgctcgacaa tccgagcgga cgtctgcgag tcgaaatgcc gggtgctgtt gcgacttcaa tcgtgctgcc cgcgctcgat gattttcacg agcgctatcc gtcgatcgat ctcgcgatcg gcgtcagtca tcgcaacgtc gatctcgtcg gcgaagcggt ggattgcagt atccagctcg gagagctgcc cgattcgggc ctggctgcca agcggctcgg gacgctcgag cacgtgacgt gtgcgagtcc tgcgtatctg gagcgttacg gcatgccgga gagtctcgac gatctgtcgg agcatgtggc cgtgaactgc atgtcggcgc tgacgggacg cgccgtcgat ttcgatttcg atgtcggcgg cagtccgttg accgtgaagg tgaacggctt catcaaggtc agcgacgagc atgcgtatct cacctgcggg ttgcaggggc ttggattgat ccagcctgcg aggatcgctg cgcaagcgtt gcttgataag ggcctgttgc gtgaggtcct gccgcagtgg aagccggtgc cgatgcccgt ttccgttgct tatgtgaagg ggcggcgtgt cgcgccgcgg gtcagagctt ttgtcgattg gctcgctgag ttgtttgagc accaggggca cgtcgaggcg gatttgtccc gggtcaggat gttgctgcag ggtcgcttgc atgcggttta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a