BuphA.12800.o

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.o
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.o.A1.GE32307 Full length( BuphA.12800.o ) 1 308
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4255
RefSeq: YP_001860201.1
UniProt: B2JPG4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRLDALRLF VEIAEAGSFS AAARKSTVST STVTLALQKL EEEVGARLIM RTTRRLAFTH EGQRFLDDAR RVLADWDAAI LSLREDGPLN GPIRLTASTD FGRTRVVPLL DKFMSLYPQV QVSLLLTDGV VDLVDQHLDL ALRHGPLIDP RLKARLLLTG PRVVCAAPSY WAAHGKAMHP SQLSSHNCLI LARPDAPFTN WPFVVDGKQI AVRVSGNRVA SDGSVLREWA VNGYGVILKN RWDVYHELIS GSLETALDQY IGERVDLFAV YAGAVPSRRV GTLIDFIARE LAVDDALELH PLDSLRPL
NT Sequence
ATGGACCGGC TTGACGCGTT AAGGCTGTTT GTCGAAATCG CCGAAGCCGG GAGTTTCTCC GCGGCTGCGC GAAAGAGCAC GGTATCGACG TCGACGGTAA CGTTAGCGCT GCAAAAGCTG GAAGAGGAAG TCGGCGCACG TCTCATCATG CGCACGACGC GGCGTTTGGC TTTTACGCAT GAAGGCCAGC GCTTTCTCGA CGACGCACGC CGTGTGCTTG CCGATTGGGA TGCGGCGATC CTGAGCCTGC GCGAAGACGG CCCATTGAAC GGGCCAATCC GCTTGACGGC CAGCACCGAT TTCGGCCGCA CACGCGTCGT GCCGTTGCTG GACAAGTTCA TGAGCCTGTA CCCACAGGTG CAGGTGTCGC TGTTGCTGAC GGACGGCGTG GTGGATCTGG TCGATCAACA TCTCGACCTG GCGTTGCGAC ATGGTCCGCT GATCGACCCG CGGCTCAAGG CACGCCTGCT GCTGACGGGA CCGCGCGTCG TATGCGCCGC TCCGTCCTAT TGGGCGGCAC ATGGCAAGGC GATGCATCCG TCGCAATTGT CCTCGCATAA CTGCCTGATC CTCGCGCGGC CCGATGCGCC GTTCACGAAC TGGCCGTTCG TCGTCGACGG CAAGCAGATC GCCGTCAGAG TATCCGGTAA CCGTGTGGCG AGCGACGGCA GCGTGCTGCG CGAGTGGGCC GTCAACGGCT ATGGCGTGAT ACTGAAGAAC CGGTGGGATG TCTATCACGA ACTGATATCA GGCAGCCTCG AGACGGCGCT GGATCAGTAC ATCGGCGAGC GCGTCGATCT GTTCGCTGTC TATGCGGGGG CGGTGCCGAG CCGGCGGGTA GGTACGCTGA TCGATTTCAT CGCGCGAGAA CTGGCCGTCG ACGACGCGCT CGAGCTGCAT CCGCTGGACA GCCTTCGACC GCTT
Details for BuphA.12800.o.A1.GE32307
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDRLDAL RLFVEIAEAG SFSAAARKST VSTSTVTLAL QKLEEEVGAR LIMRTTRRLA FTHEGQRFLD DARRVLADWD AAILSLREDG PLNGPIRLTA STDFGRTRVV PLLDKFMSLY PQVQVSLLLT DGVVDLVDQH LDLALRHGPL IDPRLKARLL LTGPRVVCAA PSYWAAHGKA MHPSQLSSHN CLILARPDAP FTNWPFVVDG KQIAVRVSGN RVASDGSVLR EWAVNGYGVI LKNRWDVYHE LISGSLETAL DQYIGERVDL FAVYAGAVPS RRVGTLIDFI ARELAVDDAL ELHPLDSLRP L
Validated NT Sequence
ttattaaagc ggtcgaaggc tgtccagcgg atgcagctcg agcgcgtcgt cgacggccag ttctcgcgcg atgaaatcga tcagcgtacc tacccgccgg ctcggcaccg cccccgcata gacagcgaac agatcgacgc gctcgccgat gtactgatcc agcgccgtct cgaggctgcc tgatatcagt tcgtgataga catcccaccg gttcttcagt atcacgccat agccgttgac ggcccactcg cgcagcacgc tgccgtcgct cgccacacgg ttaccggata ctctgacggc gatctgcttg ccgtcgacga cgaacggcca gttcgtgaac ggcgcatcgg gccgcgcgag gatcaggcag ttatgcgagg acaattgcga cggatgcatc gccttgccat gtgccgccca ataggacgga gcggcgcata cgacgcgcgg tcccgtcagc agcaggcgtg ccttgagccg cgggtcgatc agcggaccat gtcgcaacgc caggtcgaga tgttgatcga ccagatccac cacgccgtcc gtcagcaaca gcgacacctg cacctgtggg tacaggctca tgaacttgtc cagcaacggc acgacgcgtg tgcggccgaa atcggtgctg gccgtcaagc ggattggccc gttcaatggg ccgtcttcgc gcaggctcag gatcgccgca tcccaatcgg caagcacacg gcgtgcgtcg tcgagaaagc gctggccttc atgcgtaaaa gccaaacgcc gcgtcgtgcg catgatgaga cgtgcgccga cttcctcttc cagcttttgc agcgctaacg ttaccgtcga cgtcgatacc gtgctctttc gcgcagccgc ggagaaactc ccggcttcgg cgatttcgac aaacagcctt aacgcgtcaa gccggtccat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRLDALRL FVEIAEAGSF SAAARKSTVS TSTVTLALQK LEEEVGARLI MRTTRRLAFT HEGQRFLDDA RRVLADWDAA ILSLREDGPL NGPIRLTAST DFGRTRVVPL LDKFMSLYPQ VQVSLLLTDG VVDLVDQHLD LALRHGPLID PRLKARLLLT GPRVVCAAPS YWAAHGKAMH PSQLSSHNCL ILARPDAPFT NWPFVVDGKQ IAVRVSGNRV ASDGSVLREW AVNGYGVILK NRWDVYHELI SGSLETALDQ YIGERVDLFA VYAGAVPSRR VGTLIDFIAR ELAVDDALEL HPLDSLRPL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccggctt gacgcgttaa ggctgtttgt cgaaatcgcc gaagccggga gtttctccgc ggctgcgcga aagagcacgg tatcgacgtc gacggtaacg ttagcgctgc aaaagctgga agaggaagtc ggcgcacgtc tcatcatgcg cacgacgcgg cgtttggctt ttacgcatga aggccagcgc tttctcgacg acgcacgccg tgtgcttgcc gattgggatg cggcgatcct gagcctgcgc gaagacggcc cattgaacgg gccaatccgc ttgacggcca gcaccgattt cggccgcaca cgcgtcgtgc cgttgctgga caagttcatg agcctgtacc cacaggtgca ggtgtcgctg ttgctgacgg acggcgtggt ggatctggtc gatcaacatc tcgacctggc gttgcgacat ggtccgctga tcgacccgcg gctcaaggca cgcctgctgc tgacgggacc gcgcgtcgta tgcgccgctc cgtcctattg ggcggcacat ggcaaggcga tgcatccgtc gcaattgtcc tcgcataact gcctgatcct cgcgcggccc gatgcgccgt tcacgaactg gccgttcgtc gtcgacggca agcagatcgc cgtcagagta tccggtaacc gtgtggcgag cgacggcagc gtgctgcgcg agtgggccgt caacggctat ggcgtgatac tgaagaaccg gtgggatgtc tatcacgaac tgatatcagg cagcctcgag acggcgctgg atcagtacat cggcgagcgc gtcgatctgt tcgctgtcta tgcgggggcg gtgccgagcc ggcgggtagg tacgctgatc gatttcatcg cgcgagaact ggccgtcgac gacgcgctcg agctgcatcc gctggacagc cttcgaccgc tttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa