BuphA.12800.n

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.n
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.n.A1.GE32306 Full length( BuphA.12800.n ) 1 304
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4130
RefSeq: YP_001860086.1
UniProt: B2JNT9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRFEAMEIF TRVVDANSFS KVSEMLDLPR AKVSRTIQAL EEHVGVRLLN RSTRQVSVTE DGALFYERCV RILAEVADAE SSLSNKRETP AGTIRVDTSG TLARALLLPV LDDFYQRFPE IDVRLGLADR NIDLIQDGVD CVIRMGMLEE SSLVAKRIGS SRIVTCASPA YLDKHGTPRT LEELAEHQAV NYVSARTGRT FPFEYNVGDE VVKVQMKSVL AVNDGSVYIN AGALGHGIIQ PSRFMVAELI NQGALREILT EYDSPSTPLS IVYPHRRNLS SRLRAFVDWV SEIAHNNPDL RIKD
NT Sequence
ATGGATCGCT TCGAGGCAAT GGAGATCTTC ACTCGCGTGG TGGACGCGAA CAGCTTTTCA AAAGTATCTG AAATGCTCGA TTTGCCGCGC GCAAAGGTGA GCAGGACGAT TCAGGCGCTG GAGGAGCATG TCGGCGTGCG CCTTCTGAAC CGTTCGACGC GCCAGGTCAG CGTCACAGAA GACGGCGCGC TGTTTTACGA ACGATGCGTG CGCATCCTCG CTGAAGTGGC GGATGCCGAG TCGTCGCTGT CGAACAAGCG CGAGACTCCG GCGGGCACGA TTCGCGTCGA TACGTCGGGC ACGCTTGCAC GTGCATTGCT GCTGCCCGTG CTCGACGACT TTTATCAACG CTTTCCCGAG ATCGACGTGC GCCTCGGCCT CGCGGATCGC AACATCGACC TGATCCAGGA CGGCGTGGAC TGCGTGATCC GCATGGGCAT GCTCGAAGAG TCGAGTCTCG TGGCAAAGCG CATCGGCAGT TCGCGCATCG TCACATGCGC GTCGCCTGCC TATCTGGACA AACACGGCAC GCCCAGGACG CTCGAAGAAC TCGCGGAGCA CCAGGCTGTG AACTACGTAT CGGCGCGCAC GGGGAGAACC TTTCCGTTCG AGTACAACGT CGGCGATGAA GTGGTGAAGG TGCAGATGAA GAGCGTGCTC GCCGTCAACG ACGGTTCGGT GTATATCAAT GCGGGCGCGC TCGGTCACGG CATCATCCAG CCGTCGCGCT TCATGGTGGC CGAGTTGATC AACCAGGGCG CGCTGCGCGA AATCCTCACC GAGTACGACA GCCCGTCGAC GCCGCTTTCG ATCGTTTATC CGCATCGCCG CAATCTCAGT TCGCGCCTGC GCGCGTTCGT CGACTGGGTG TCGGAGATTG CGCACAACAA TCCCGACCTG CGCATCAAGG AC
Details for BuphA.12800.n.A1.GE32306
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 105
Validated AA Sequence
MGTLEAQTQG PGSMDRFEAM EIFTRVVDAN SFSKVSEMLD LPRAKVSRTI QALEEHVGVR LLNRSTRQVS VTEDGALFYE RCVRILAEVA DAESSLSNKR ETPAGTIRVD TSGTLARALL LPVLDDFYQR FPEIDVRLGL ADRNIDLIQD GVDCVIRMGM LEESSLVAKR IGSSRIVTCA SPAYLDKHGT PRTLEELAEH QAVNYVSART GRTFPFEYNV GDEVVKVQMK SVLAVNDGSV YINAGALGHG IIQPSRFMVA ELINQGALRE ILTEYDSPST PLSIVYPHRR NLSSRLRAFV DWVSXIAHNN PXLRIXTNKQ HEXXAPSFXX XXLLXKXEXX LXC
Validated NT Sequence
attttgttta ctttaagaag gagatatacc ntggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggatc gcttcgaggc aatggagatc ttcactcgcg tggtggacgc gaacagcttt tcaaaagtat ctgaaatgct cgatttgccg cgcgcaaagg tgagcaggac gattcaggcg ctggaggagc atgtcggcgt gcgccttctg aaccgttcga cgcgccaggt cagcgtcaca gaagacggcg cgctgtttta cgaacgatgc gtgcgcatcc tcgctgaagt ggcggatgcc gagtcgtcgc tgtcgaacaa gcgcgagact ccggcgggca cgattcgcgt cgatacgtcg ggcacgcttg cacgtgcatt gctgctgccc gtgctcgacg acttttatca acgctttccc gagatcgacg tgcgcctcgg cctcgcggat cgcaacatcg acctgatcca ggacggcgtg gactgcgtga tccgcatggg catgctcgaa gagtcgagtc tcgtggcaaa gcgcatcggc agttcgcgca tcgtcacatg cgcgtcgcct gcctatctgg acaaacacgg cacgcccagg acgctcgaag aactcgcgga gcaccaggct gtgaactacg tatcggcgcg cacggggaga acctttccgt tcgagtacaa cgtcggcgat gaagtggtga aggtgcagat gaagagcgtg ctcgccgtca acgacggttc ggtgtatatc aatgcgggcg cgctcggtca cggcatcatc cagccgtcgc gcttcatggt ggccgagttg atcaaccagg gcgcgctgcg cgaaatcctc accgagtacg acagcccgtc gacgccgctt tcgatcgttt atccgcatcg ccgcaatctc agttcgcgcc tgcgcgcgtt cgtcgactgg gtgtcngana ttgcgcacaa caatccngan ctgcgcatcn nnactaataa acagcacgaa nantntgcac cnagcttcnn nnngntccnn ctgctancaa agncngaann gnagctgnnn tgctg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDRFEAMEI FTRVVDANSF SKVSEMLDLP RAKVSRTIQA LEEHVGVRLL NRSTRQVSVT EDGALFYERC VRILAEVADA ESSLSNKRET PAGTIRVDTS GTLARALLLP VLDDFYQRFP EIDVRLGLAD RNIDLIQDGV DCVIRMGMLE ESSLVAKRIG SSRIVTCASP AYLDKHGTPR TLEELAEHQA VNYVSARTGR TFPFEYNVGD EVVKVQMKSV LAVNDGSVYI NAGALGHGII QPSRFMVAEL INQGALREIL TEYDSPSTPL SIVYPHRRNL SSRLRAFVDW VSEIAHNNPD LRIKD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatcgcttc gaggcaatgg agatcttcac tcgcgtggtg gacgcgaaca gcttttcaaa agtatctgaa atgctcgatt tgccgcgcgc aaaggtgagc aggacgattc aggcgctgga ggagcatgtc ggcgtgcgcc ttctgaaccg ttcgacgcgc caggtcagcg tcacagaaga cggcgcgctg ttttacgaac gatgcgtgcg catcctcgct gaagtggcgg atgccgagtc gtcgctgtcg aacaagcgcg agactccggc gggcacgatt cgcgtcgata cgtcgggcac gcttgcacgt gcattgctgc tgcccgtgct cgacgacttt tatcaacgct ttcccgagat cgacgtgcgc ctcggcctcg cggatcgcaa catcgacctg atccaggacg gcgtggactg cgtgatccgc atgggcatgc tcgaagagtc gagtctcgtg gcaaagcgca tcggcagttc gcgcatcgtc acatgcgcgt cgcctgccta tctggacaaa cacggcacgc ccaggacgct cgaagaactc gcggagcacc aggctgtgaa ctacgtatcg gcgcgcacgg ggagaacctt tccgttcgag tacaacgtcg gcgatgaagt ggtgaaggtg cagatgaaga gcgtgctcgc cgtcaacgac ggttcggtgt atatcaatgc gggcgcgctc ggtcacggca tcatccagcc gtcgcgcttc atggtggccg agttgatcaa ccagggcgcg ctgcgcgaaa tcctcaccga gtacgacagc ccgtcgacgc cgctttcgat cgtttatccg catcgccgca atctcagttc gcgcctgcgc gcgttcgtcg actgggtgtc ggagattgcg cacaacaatc ccgacctgcg catcaaggac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa