BuphA.12800.m

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.m
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.m.A1.GE32305 Full length( BuphA.12800.m ) 1 296
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4120
RefSeq: YP_001860076.1
UniProt: B2JNS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLDDLKIFV TTVDARNFTA AANRLQLSKQ FVSRRVMALE ASLGVRLLVR NTRKLAVTDL GYELYQRATR ILGEVEDAEQ AMTSGRSDPR GLLRVSAPMS FGMIHLSPLV AAFLRANPDV RVDMELSDRV VDVVGEGFDM ALRIGTLADS TLIAQKLAEI RMVACCSPAY RKARRPPATP GDLARHACLL YGQEGRSGWE YMVDGASRTI EVHGPLRVNN GEVIRDAAIA GLGIARLPEF IVAEALASGK LVEVLGDFVS PPLTLYAVYP QHRQASITIR AFTQFLRDRF VKAIRR
NT Sequence
ATGCAACTGG ACGATCTGAA AATCTTCGTC ACAACCGTCG ACGCACGCAA TTTCACGGCG GCGGCCAACC GGCTGCAACT GTCGAAGCAG TTCGTCAGCC GCCGCGTGAT GGCGCTCGAA GCAAGCCTCG GCGTGCGGCT GCTGGTGCGA AACACGCGCA AGCTCGCGGT GACGGACCTC GGCTATGAGC TCTACCAGCG CGCGACGCGC ATCCTCGGCG AGGTGGAGGA CGCGGAGCAG GCGATGACGT CGGGCCGTTC GGACCCGCGT GGGCTGTTGC GCGTCAGCGC GCCGATGTCG TTTGGGATGA TCCATTTGTC GCCCCTCGTC GCCGCGTTTC TGCGCGCCAA TCCCGACGTG CGGGTGGACA TGGAGTTGAG CGATCGCGTC GTCGATGTGG TCGGCGAGGG CTTCGACATG GCGCTGCGCA TCGGCACGCT GGCGGACTCG ACGCTGATTG CGCAGAAGCT CGCTGAGATA CGCATGGTTG CGTGTTGCAG TCCCGCGTAT CGCAAGGCGC GCCGTCCGCC CGCGACGCCC GGCGATCTCG CGCGTCACGC GTGCCTGCTC TATGGACAGG AAGGGCGCAG CGGTTGGGAG TACATGGTGG ACGGCGCGAG CAGGACAATC GAAGTACATG GCCCGCTGCG CGTGAACAAT GGCGAAGTGA TCCGCGACGC GGCTATAGCA GGGCTTGGCA TTGCGCGTCT GCCGGAGTTC ATCGTCGCCG AAGCGCTCGC GAGCGGCAAG CTCGTCGAAG TGCTCGGCGA TTTCGTCTCG CCTCCGTTGA CGCTCTATGC GGTGTATCCG CAGCACCGTC AGGCTTCCAT CACGATACGC GCGTTCACGC AGTTCTTACG CGACCGCTTC GTCAAGGCGA TACGTCGC
Details for BuphA.12800.m.A1.GE32305
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQLDDLKIF VTTVDARNFT AAANRLQLSK QFVSRRVMAL EASLGVRLLV RNTRKLAVTD LGYELYQRAT RILGEVEDAE QAMTSGRSDP RGLLRVSAPM SFGMIHLSPL VAAFLRANPD VRVDMELSDR VVDVVGEGFD MALRIGTLAD STLIAQKLAE IRMVACCSPA YRKARRPPAT PGDLARHACL LYGQEGRSGW EYMVDGASRT IEVHGPLRVN NGEVIRDAAI AGLGIARLPE FIVAEALASG KLVEVLGDFV SPPLTLYAVY PQHRQASITI RAFTQFLRDR FVKAIR
Validated NT Sequence
gcgacgtatc gccttgacga agcggtcgcg taagaactgc gtgaacgcgc gtatcgtgat ggaagcctga cggtgctgcg gatacaccgc atagagcgtc aacggaggcg agacgaaatc gccgagcact tcgacgagct tgccgctcgc gagcgcttcg gcgacgatga actccggcag acgcgcaatg ccaagccctg ctatagccgc gtcgcggatc acttcgccat tgttcacgcg cagcgggcca tgtacttcga ttgtcctgct cgcgccgtcc accatgtact cccaaccgct gcgcccttcc tgtccataga gcaggcacgc gtgacgcgcg agatcgccgg gcgtcgcggg cggacggcgc gccttgcgat acgcgggact gcaacacgca accatgcgta tctcagcgag cttctgcgca atcagcgtcg agtccgccag cgtgccgatg cgcagcgcca tgtcgaagcc ctcgccgacc acatcgacga cgcgatcgct caactccatg tccacccgca cgtcgggatt ggcgcgcaga aacgcggcga cgaggggcga caaatggatc atcccaaacg acatcggcgc gctgacgcgc aacagcccac gcgggtccga acggcccgac gtcatcgcct gctccgcgtc ctccacctcg ccgaggatgc gcgtcgcgcg ctggtagagc tcatagccga ggtccgtcac cgcgagcttg cgcgtgtttc gcaccagcag ccgcacgccg aggcttgctt cgagcgccat cacgcggcgg ctgacgaact gcttcgacag ttgcagccgg ttggccgccg ccgtgaaatt gcgtgcgtcg acggttgtga cgaagatttt cagatcgtcc agttgcatcg aaccaggacc ctgggtctga gcttccaggg tacccatatg atggtgatgg tgatgagcca tggtat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQLDDLKIF VTTVDARNFT AAANRLQLSK QFVSRRVMAL EASLGVRLLV RNTRKLAVTD LGYELYQRAT RILGEVEDAE QAMTSGRSDP RGLLRVSAPM SFGMIHLSPL VAAFLRANPD VRVDMELSDR VVDVVGEGFD MALRIGTLAD STLIAQKLAE IRMVACCSPA YRKARRPPAT PGDLARHACL LYGQEGRSGW EYMVDGASRT IEVHGPLRVN NGEVIRDAAI AGLGIARLPE FIVAEALASG KLVEVLGDFV SPPLTLYAVY PQHRQASITI RAFTQFLRDR FVKAIRR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaactggac gatctgaaaa tcttcgtcac aaccgtcgac gcacgcaatt tcacggcggc ggccaaccgg ctgcaactgt cgaagcagtt cgtcagccgc cgcgtgatgg cgctcgaagc aagcctcggc gtgcggctgc tggtgcgaaa cacgcgcaag ctcgcggtga cggacctcgg ctatgagctc taccagcgcg cgacgcgcat cctcggcgag gtggaggacg cggagcaggc gatgacgtcg ggccgttcgg acccgcgtgg gctgttgcgc gtcagcgcgc cgatgtcgtt tgggatgatc catttgtcgc ccctcgtcgc cgcgtttctg cgcgccaatc ccgacgtgcg ggtggacatg gagttgagcg atcgcgtcgt cgatgtggtc ggcgagggct tcgacatggc gctgcgcatc ggcacgctgg cggactcgac gctgattgcg cagaagctcg ctgagatacg catggttgcg tgttgcagtc ccgcgtatcg caaggcgcgc cgtccgcccg cgacgcccgg cgatctcgcg cgtcacgcgt gcctgctcta tggacaggaa gggcgcagcg gttgggagta catggtggac ggcgcgagca ggacaatcga agtacatggc ccgctgcgcg tgaacaatgg cgaagtgatc cgcgacgcgg ctatagcagg gcttggcatt gcgcgtctgc cggagttcat cgtcgccgaa gcgctcgcga gcggcaagct cgtcgaagtg ctcggcgatt tcgtctcgcc tccgttgacg ctctatgcgg tgtatccgca gcaccgtcag gcttccatca cgatacgcgc gttcacgcag ttcttacgcg accgcttcgt caaggcgata cgtcgctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa