BuphA.12800.i

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.i
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.i.A1.GE32301 Full length( BuphA.12800.i ) 1 320
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.5263
RefSeq: YP_001861139.1
UniProt: B2JLC5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKFREMEVF VAIVDRGSFT GASEKLGMSA PTVSRALHSL ETRVGAQLIA RTTRSIRPTD AGMFYLDACR SVLDTITDAE ANISAGQDKP AGTLTVSAPV LFGQRFIAPL INAYVNSYPD VSVKVVYVDR TTRLIEEGVD IAVRIGHLGD SSVFAVPLGS VCRRTYAAPA YLDAYGEPVH PKDIGRHHCV SYTGVTHPLE WLFCENGSRL PVRIRPRMIV DLASAAVMAA VDRVGITQLL SYQAAPELLS GSLQRILAAF EPESIPVNLL HVERKSSSMK IRSFVEFVTE TLRRNVHLQF VDLQKSVRAV TAIADTLECE
NT Sequence
ATGGACAAAT TTCGCGAGAT GGAGGTGTTT GTCGCCATCG TTGATCGAGG CAGTTTTACA GGTGCGTCGG AAAAGCTCGG CATGTCGGCT CCGACGGTTT CGCGCGCGCT GCATTCGCTC GAGACGCGAG TGGGCGCGCA GTTGATCGCG CGCACCACGC GTTCTATCCG TCCCACAGAT GCGGGAATGT TCTACCTCGA TGCGTGCCGC AGCGTACTCG ATACGATCAC CGACGCGGAG GCAAATATTT CCGCCGGGCA AGACAAGCCT GCGGGGACGT TGACGGTGTC GGCGCCGGTG CTGTTCGGCC AACGGTTTAT CGCGCCTCTC ATCAACGCAT ACGTGAATAG TTATCCCGAC GTCAGTGTGA AGGTAGTCTA TGTCGATCGC ACCACGCGCC TTATCGAGGA AGGCGTGGAC ATTGCGGTTC GCATCGGTCA TCTCGGCGAT TCTTCTGTCT TCGCGGTGCC GCTTGGCTCC GTGTGCCGTC GTACTTACGC AGCGCCGGCG TATCTCGACG CATACGGCGA ACCCGTCCAT CCGAAGGATA TCGGCAGGCA CCACTGCGTG TCGTATACGG GCGTCACGCA TCCGCTTGAA TGGCTGTTTT GCGAGAACGG GTCAAGGCTG CCCGTTCGCA TTCGTCCTCG CATGATCGTC GACCTCGCGT CGGCCGCTGT CATGGCGGCC GTCGATCGCG TGGGAATCAC GCAATTGCTT TCGTATCAGG CGGCCCCCGA GTTGCTGAGC GGGAGCCTTC AACGGATTCT CGCCGCATTC GAGCCGGAAT CGATACCGGT GAACCTGCTG CACGTCGAGC GCAAAAGCTC AAGCATGAAG ATCCGATCGT TCGTCGAATT CGTCACGGAA ACGCTGCGCA GAAACGTGCA TCTTCAATTC GTCGACCTTC AAAAGAGTGT GCGTGCAGTA ACTGCAATCG CAGACACGCT GGAGTGCGAG
Details for BuphA.12800.i.A1.GE32301
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 78
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMDKFREMEV FVAIVDRGSF TGASEKLGMS APTVSRALHS LETRVGAQLI ARTTRSIRPT DAGMFYLDAC RSVLDTITDA EANISAGQDK PAGTLTVSAP VLFGQRFIAP LINAYVNSYP DVSVKVVYVD RTTRLIEEGV DIAVRIGHLG DSSVFAVPLG SVCRRTYAAP AYLDAYGEPV HPKDIGRHHC VSYTGVTHPL EWLFCENGSR LPVRIRPRMI VDLASAAVMA AVDRVGITQL LSYXXPPSC
Validated NT Sequence
attttgtnnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaca aatttcgcga gatggaggtg tttgtcgcca tcgttgatcg aggcagtttt acaggtgcgt cggaaaagct cggcatgtcg gctccgacgg tttcgcgcgc gctgcattcg ctcgagacgc gagtgggcgc gcagttgatc gcgcgcacca cgcgttctat ccgtcccaca gatgcgggaa tgttctacct cgatgcgtgc cgcagcgtac tcgatacgat caccgacgcg gaggcaaata tttccgccgg gcaagacaag cctgcgggga cgttgacggt gtcggcgccg gtgctgttcg gccaacggtt tatcgcgcct ctcatcaacg catacgtgaa tagttatccc gacgtcagtg tgaaggtagt ctatgtcgat cgcaccacgc gccttatcga ggaaggcgtg gacattgcgg ttcgcatcgg tcatctcggc gattcttctg tcttcgcggt gccgcttggc tccgtgtgcc gtcgtactta cgcagcgccg gcgtatctcg acgcatacgg cgaacccgtc catccgaagg atatcggcag gcaccactgc gtgtcgtata cgggcgtcac gcatccgctt gaatggctgt tttgcgagaa cgggtcaagg ctgcccgttc gcattcgtcc tcgcatgatc gtcgacctcg cgtcggccgc tgtcatggcg gccgtcgatc gcgtgggaat cacgcaattg ctttcgtatc nnnggccccc gagttgctga gcgggagcct tcaacggatt ctcgccgcat tcgagccgga atcgataccg gtgaacctgc tgcacgtcga gcgcaaaagc tcaagcatga agatccnatc gttcgtcgaa ttcgtcacgg aaacgctgcg cagaaacgtg catcttcaat tcgtcgacct tcaaaagant gtgcgtgcag taactgcaat cnnnnanncn ntggnnnnnn nnagtantna anngcannnn nntnnngcan nnnctnnnnn nagnatnnnn ctgctnnnan ccnnnnnctn nnnnnnnnnn nnnnnnanan nnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKFREMEV FVAIVDRGSF TGASEKLGMS APTVSRALHS LETRVGAQLI ARTTRSIRPT DAGMFYLDAC RSVLDTITDA EANISAGQDK PAGTLTVSAP VLFGQRFIAP LINAYVNSYP DVSVKVVYVD RTTRLIEEGV DIAVRIGHLG DSSVFAVPLG SVCRRTYAAP AYLDAYGEPV HPKDIGRHHC VSYTGVTHPL EWLFCENGSR LPVRIRPRMI VDLASAAVMA AVDRVGITQL LSYQAAPELL SGSLQRILAA FEPESIPVNL LHVERKSSSM KIRSFVEFVT ETLRRNVHLQ FVDLQKSVRA VTAIADTLEC E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaaattt cgcgagatgg aggtgtttgt cgccatcgtt gatcgaggca gttttacagg tgcgtcggaa aagctcggca tgtcggctcc gacggtttcg cgcgcgctgc attcgctcga gacgcgagtg ggcgcgcagt tgatcgcgcg caccacgcgt tctatccgtc ccacagatgc gggaatgttc tacctcgatg cgtgccgcag cgtactcgat acgatcaccg acgcggaggc aaatatttcc gccgggcaag acaagcctgc ggggacgttg acggtgtcgg cgccggtgct gttcggccaa cggtttatcg cgcctctcat caacgcatac gtgaatagtt atcccgacgt cagtgtgaag gtagtctatg tcgatcgcac cacgcgcctt atcgaggaag gcgtggacat tgcggttcgc atcggtcatc tcggcgattc ttctgtcttc gcggtgccgc ttggctccgt gtgccgtcgt acttacgcag cgccggcgta tctcgacgca tacggcgaac ccgtccatcc gaaggatatc ggcaggcacc actgcgtgtc gtatacgggc gtcacgcatc cgcttgaatg gctgttttgc gagaacgggt caaggctgcc cgttcgcatt cgtcctcgca tgatcgtcga cctcgcgtcg gccgctgtca tggcggccgt cgatcgcgtg ggaatcacgc aattgctttc gtatcaggcg gcccccgagt tgctgagcgg gagccttcaa cggattctcg ccgcattcga gccggaatcg ataccggtga acctgctgca cgtcgagcgc aaaagctcaa gcatgaagat ccgatcgttc gtcgaattcg tcacggaaac gctgcgcaga aacgtgcatc ttcaattcgt cgaccttcaa aagagtgtgc gtgcagtaac tgcaatcgca gacacgctgg agtgcgagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a