SSGCID: Target Page

BuphA.12800.g

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.g
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.g.A1.GE34735	Full length( BuphA.12800.g )		1	312

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.166
RefSeq:	YP_001856394.1
UniProt:	B2JKF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDTLVSMKVF RHVVEVGSFV GASEKMDMSA AMASKHVMHL EQQLGARLLN RTTRRVAPTE AGREYYERLS QVLSELDEAE QAVGAASVVP QGRLRVSSLS AFGLRHVMSA VADYATQHPQ VTVDITLSDR VVELIDEGFD VAIRASPSGL KSSSLIARQV ATAHLVLCAS PEYIERHGAP KSAADLTRHN IIQYSGVSAL ELVAGANAES GRVKLSGNLI VNHLEAQRVV VLQGAALALL GTEVIGDDLA AGRVVPLLID ELPPRELPIH VVYASRRHLS AKVRSFVDFI AERFSSEPLW PTLEQIKALA LR

NT Sequence

ATGGATACCC TCGTCAGCAT GAAAGTGTTT CGCCACGTCG TCGAGGTCGG CAGCTTTGTC GGCGCGTCGG AGAAAATGGA CATGTCGGCG GCGATGGCGA GCAAGCATGT GATGCATCTC GAACAGCAGC TCGGCGCGCG GCTGTTGAAT CGCACCACGC GCCGCGTCGC GCCGACCGAG GCGGGCCGCG AGTACTACGA GCGCCTGAGC CAGGTGTTGA GCGAACTAGA CGAAGCGGAA CAGGCAGTGG GCGCCGCGAG CGTCGTGCCG CAAGGGCGGT TGCGCGTGTC GTCGCTATCG GCGTTCGGAC TGCGCCATGT GATGAGCGCC GTCGCCGATT ATGCGACGCA GCATCCTCAG GTCACCGTCG ACATCACGCT GTCGGACCGC GTTGTCGAAC TGATCGACGA AGGCTTCGAT GTCGCGATAC GCGCGTCGCC GTCGGGACTC AAGTCGTCCT CGCTGATCGC GCGTCAGGTC GCGACTGCGC ACCTGGTGCT GTGTGCGTCG CCGGAATATA TCGAGCGGCA CGGCGCACCG AAATCTGCCG CTGATCTGAC ACGCCACAAC ATCATTCAGT ACTCAGGCGT ATCGGCGCTC GAACTCGTGG CGGGCGCGAA TGCCGAGAGC GGGCGCGTCA AGCTGTCGGG CAATCTGATC GTCAATCATC TGGAGGCGCA GCGCGTCGTT GTGCTGCAGG GCGCGGCGCT CGCGCTGCTC GGCACCGAAG TTATCGGCGA CGATCTCGCG GCGGGCCGCG TCGTGCCGTT GCTGATCGAC GAACTGCCGC CGCGCGAGCT GCCGATCCAC GTCGTCTACG CGAGCCGCCG GCACCTGTCG GCGAAGGTGC GCTCGTTCGT CGATTTCATC GCGGAGCGTT TTTCAAGCGA GCCGCTCTGG CCGACGCTCG AGCAGATCAA GGCGCTCGCG CTGCGC

Details for BuphA.12800.g.A1.GE34735

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	36kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	78

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDTLVSMKV FRHVVEVGSF VGASEKMDMS AAMASKHVMH LEQQLGARLL NRTTRRVAPT EAGREYYERL SQVLSELDEA EQAVGAASVV PQGRLRVSSL SAFGLRHVMS AVADYATQHP QVTVDITLSD RVVELIDEGF DVAIRASPSG LKSSSLIARQ VATAHLVLCA SPEYIERHGA PKSAADLTRH NIIQYSGVSA LELVAGANAE SGRVKLSGNL IVNHLEAXRV VVLXGAALAL R

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggata ccctcgtcag catgaaagtg tttcgccacg tcgtcgaggt cggcagcttt gtcggcgcgt cggagaaaat ggacatgtcg gcggcgatgg cgagcaagca tgtgatgcat ctcgaacagc agctcggcgc gcggctgttg aatcgcacca cgcgccgcgt cgcgccgacc gaggcgggcc gcgagtacta cgagcgcctg agccaggtgt tgagcgaact agacgaagcg gaacaggcag tgggcgccgc gagcgtcgtg ccgcaagggc ggttgcgcgt gtcgtcgcta tcggcgttcg gactgcgcca tgtgatgagc gccgtcgccg attatgcgac gcagcatcct caggtcaccg tcgacatcac gctgtcggac cgcgttgtcg aactgatcga cgaaggcttc gatgtcgcga tacgcgcgtc gccgtcggga ctcaagtcgt cctcgctgat cgcgcgtcag gtcgcgactg cgcacctggt gctgtgtgcg tcgccggaat atatcgagcg gcacggcgca ccgaaatctg ccgctgatct gacacgccac aacatcattc agtactcagg cgtatcggcg ctcgaactcg tggcgggcgc gaatgccgag agcgggcgcg tcaagctgtc gggcaatctg atcgtcaatc atctggaggc gcancgcgtc gttgtgctgc nnggcgcggc gctcgcgctg cgctaataa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggataccctc gtcagcatga aagtgtttcg ccacgtcgtc gaggtcggca gctttgtcgg cgcgtcggag aaaatggaca tgtcggcggc gatggcgagc aagcatgtga tgcatctcga acagcagctc ggcgcgcggc tgttgaatcg caccacgcgc cgcgtcgcgc cgaccgaggc gggccgcgag tactacgagc gcctgagcca ggtgttgagc gaactagacg aagcggaaca ggcagtgggc gccgcgagcg tcgtgccgca agggcggttg cgcgtgtcgt cgctatcggc gttcggactg cgccatgtga tgagcgccgt cgccgattat gcgacgcagc atcctcaggt caccgtcgac atcacgctgt cggaccgcgt tgtcgaactg atcgacgaag gcttcgatgt cgcgatacgc gcgtcgccgt cgggactcaa gtcgtcctcg ctgatcgcgc gtcaggtcgc gactgcgcac ctggtgctgt gtgcgtcgcc ggaatatatc gagcggcacg gcgcaccgaa atctgccgct gatctgacac gccacaacat cattcagtac tcaggcgtat cggcgctcga actcgtggcg ggcgcgaatg ccgagagcgg gcgcgtcaag ctgtcgggca atctgatcgt caatcatctg gaggcgcagc gcgtcgttgt gctgcagggc gcggcgctcg cgctgctcgg caccgaagtt atcggcgacg atctcgcggc gggccgcgtc gtgccgttgc tgatcgacga actgccgccg cgcgagctgc cgatccacgt cgtctacgcg agccgccggc acctgtcggc gaaggtgcgc tcgttcgtcg atttcatcgc ggagcgtttt tcaagcgagc cgctctggcc gacgctcgag cagatcaagg cgctcgcgct gcgctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa