BuphA.12800.e

Transcriptional regulator, LysR family

CENTER ID: BuphA.12800.e
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12800.e.A1.GE34734 Full length( BuphA.12800.e ) 1 312
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1691
RefSeq: YP_001857809.1
UniProt: B2JK40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLQNMRVF VRVVEAGSFT GAAQHLNTTT AYASRAVSDL EAHLRTRLLN RTTRRIALTE AGERYLQRCE QILAYVDQAE AEASDAHARP SGKLKVHAMT SFGQHYVVPA VGRYQQRYPD VHLELTLAQR MPDLLDEGYD VALVLAQSLP DSGLVSQRLG SAFSIACASP DYLERNGVPQ TLSDLRGHTC LQMITPVMPA DEWTFDGPNG QETLHLGAST FQINVAEAMA VAVREGMGVA VLPIYSAIAG LRSGELVWIL PEYISQEMNV YALYPSRQYL DAKIRTWVEF LRDELPATLA TDQAELRQFA RT
NT Sequence
ATGGATACGC TTCAGAACAT GCGCGTATTT GTCCGCGTCG TCGAAGCGGG CAGCTTTACG GGCGCGGCGC AGCATCTCAA TACGACGACG GCTTATGCGT CGCGCGCCGT GTCCGATCTC GAAGCGCATC TGCGCACGCG TCTCCTGAAC CGCACGACGC GCCGCATCGC GCTAACGGAA GCGGGCGAGC GCTATCTGCA GCGCTGCGAG CAGATTCTTG CGTATGTCGA CCAGGCGGAA GCCGAGGCCA GCGACGCGCA TGCGCGCCCG TCGGGCAAGC TGAAAGTTCA CGCGATGACG AGCTTCGGCC AGCACTACGT GGTGCCCGCC GTGGGCCGAT ATCAGCAGCG TTATCCCGAT GTCCATCTCG AACTGACGCT CGCGCAACGT ATGCCAGACC TGCTGGACGA AGGGTACGAC GTCGCGCTCG TGCTCGCGCA GAGCCTGCCG GACTCCGGGC TGGTGTCGCA GCGTCTGGGC AGTGCGTTCA GCATCGCGTG CGCTTCGCCC GATTATCTGG AGCGCAATGG CGTGCCGCAG ACGCTGTCCG ATCTGCGCGG CCACACGTGT CTGCAGATGA TCACGCCCGT GATGCCCGCC GACGAGTGGA CCTTCGACGG CCCGAACGGC CAGGAAACGC TGCATCTGGG TGCGTCGACG TTCCAGATCA ACGTCGCCGA GGCGATGGCT GTGGCAGTGC GCGAAGGCAT GGGGGTCGCC GTGCTGCCTA TCTATTCCGC GATTGCCGGT CTGCGTAGCG GCGAACTCGT GTGGATTCTG CCGGAGTACA TCTCGCAGGA GATGAACGTC TACGCGCTGT ATCCGTCGCG GCAGTATCTG GATGCGAAGA TCCGCACGTG GGTCGAGTTC CTGCGCGACG AACTTCCCGC GACGCTCGCC ACCGACCAGG CCGAACTGCG CCAGTTCGCG CGGACT
Details for BuphA.12800.e.A1.GE34734
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDTLQNM RVFVRVVEAG SFTGAAQHLN TTTAYASRAV SDLEAHLRTR LLNRTTRRIA LTEAGERYLQ RCEQILAYVD QAEAEASDAH ARPSGKLKVH AMTSFGQHYV VPAVGRYQQR YPDVHLELTL AQRMPDLLDE GYDVALVLAQ SLPDSGLVSQ RLGSAFSIAC ASPDYLERNG VPQTLSDLRG HTCLQMITPV MPADEWTFDG PNGQETLHLG ASTFQINVAE AMAVAVREGM GVAVLPIYSA IAGLRSGELV WILPEYISQE MNVYALYPSR QYLDAKIRTW VEFLRDELPA TLATDQAELR QFAR
Validated NT Sequence
tccgcgcgaa ctggcgcagt tcggcctggt cggtggcgag cgtcgcggga agttcgtcgc gcaggaactc gacccacgtg cggatcttcg catccagata ctgccgcgac ggatacagcg cgtagacgtt catctcctgc gagatgtact ccggcagaat ccacacgagt tcgccgctac gcagaccggc aatcgcggaa tagataggca gcacggcgac ccccatgcct tcgcgcactg ccacagccat cgcctcggcg acgttgatct ggaacgtcga cgcacccaga tgcagcgttt cctggccgtt cgggccgtcg aaggtccact cgtcggcggg catcacgggc gtgatcatct gcagacacgt gtggccgcgc agatcggaca gcgtctgcgg cacgccattg cgctccagat aatcgggcga agcgcacgcg atgctgaacg cactgcccag acgctgcgac accagcccgg agtccggcag gctctgcgcg agcacgagcg cgacgtcgta cccttcgtcc agcaggtctg gcatacgttg cgcgagcgtc agttcgagat ggacatcggg ataacgctgc tgatatcggc ccacggcggg caccacgtag tgctggccga agctcgtcat cgcgtgaact ttcagcttgc ccgacgggcg cgcatgcgcg tcgctggcct cggcttccgc ctggtcgaca tacgcaagaa tctgctcgca gcgctgcaga tagcgctcgc ccgcttccgt tagcgcgatg cggcgcgtcg tgcggttcag gagacgcgtg cgcagatgcg cttcgagatc ggacacggcg cgcgacgcat aagccgtcgt cgtattgaga tgctgcgccg cgcccgtaaa gctgcccgct tcgacgacgc ggacaaatac gcgcatgttc tgaagcgtat ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FVRVVEAGSF TGAAQHLNTT TAYASRAVSD LEAHLRTRLL NRTTRRIALT EAGERYLQRC EQILAYVDQA EAEASDAHAR PSGKLKVHAM TSFGQHYVVP AVGRYQQRYP DVHLELTLAQ RMPDLLDEGY DVALVLAQSL PDSGLVSQRL GSAFSIACAS PDYLERNGVP QTLSDLRGHT CLQMITPVMP ADEWTFDGPN GQETLHLGAS TFQINVAEAM AVAVREGMGV AVLPIYSAIA GLRSGELVWI LPEYISQEMN VYALYPSRQY LDAKIRTWVE FLRDELPATL ATDQAELRQF ART
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgctt cagaacatgc gcgtatttgt ccgcgtcgtc gaagcgggca gctttacggg cgcggcgcag catctcaata cgacgacggc ttatgcgtcg cgcgccgtgt ccgatctcga agcgcatctg cgcacgcgtc tcctgaaccg cacgacgcgc cgcatcgcgc taacggaagc gggcgagcgc tatctgcagc gctgcgagca gattcttgcg tatgtcgacc aggcggaagc cgaggccagc gacgcgcatg cgcgcccgtc gggcaagctg aaagttcacg cgatgacgag cttcggccag cactacgtgg tgcccgccgt gggccgatat cagcagcgtt atcccgatgt ccatctcgaa ctgacgctcg cgcaacgtat gccagacctg ctggacgaag ggtacgacgt cgcgctcgtg ctcgcgcaga gcctgccgga ctccgggctg gtgtcgcagc gtctgggcag tgcgttcagc atcgcgtgcg cttcgcccga ttatctggag cgcaatggcg tgccgcagac gctgtccgat ctgcgcggcc acacgtgtct gcagatgatc acgcccgtga tgcccgccga cgagtggacc ttcgacggcc cgaacggcca ggaaacgctg catctgggtg cgtcgacgtt ccagatcaac gtcgccgagg cgatggctgt ggcagtgcgc gaaggcatgg gggtcgccgt gctgcctatc tattccgcga ttgccggtct gcgtagcggc gaactcgtgt ggattctgcc ggagtacatc tcgcaggaga tgaacgtcta cgcgctgtat ccgtcgcggc agtatctgga tgcgaagatc cgcacgtggg tcgagttcct gcgcgacgaa cttcccgcga cgctcgccac cgaccaggcc gaactgcgcc agttcgcgcg gacttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa