SSGCID: Target Page

BuphA.12800.d

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.d
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.d.A1.GE32169	Full length( BuphA.12800.d )		1	300

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.1457
RefSeq:	YP_001857598.1
UniProt:	B2JIF8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFKQIETF VTVAARGSLS AAAQAEGVAP AIIGRRIDAL EERLGVKLLV RTTRRITLTY EGSAFLEDCQ RIIHDMQNAE ASVSAGGVKA SGHLRLSAPA GFGRRHVAPL VPAFTVAHPD VSISLDLSDR MVDLVNEGFD CAIRLGELPD SSLVSLKLGE NRRVCVASPA YLARRAAPQT LADLAHHNCL ALGASANQQR GWVFQQGDKV VTIKVSGTME CSDGAVLHEW CLEGYGLAWR SWWEVGADIA AGSLVSVLDN HAAPPIGIHA VFPQRRHLPL RVRLFLDFLK HTYGNPAYWI

NT Sequence

ATGGACCGTT TCAAGCAGAT CGAGACCTTC GTGACCGTCG CGGCGCGCGG CAGCCTGTCG GCGGCAGCGC AGGCGGAAGG CGTAGCGCCT GCCATCATCG GGCGCCGTAT CGACGCGCTC GAGGAACGCC TCGGCGTCAA GCTGCTCGTG CGCACCACGC GCCGCATCAC ATTGACCTAC GAAGGCTCCG CCTTTCTCGA AGACTGTCAG CGGATCATTC ACGACATGCA GAACGCCGAA GCGAGCGTGT CGGCGGGCGG CGTCAAGGCG AGCGGGCATT TGCGCTTGTC GGCGCCAGCG GGCTTCGGCC GCAGACACGT CGCACCGCTC GTGCCTGCGT TCACGGTGGC GCATCCCGAC GTGTCGATTT CTCTCGACCT GTCCGACCGC ATGGTCGACC TCGTCAACGA AGGATTCGAT TGCGCGATCC GGCTCGGCGA ACTTCCGGAT TCGTCGCTGG TGTCGTTGAA GCTCGGTGAG AACCGGCGTG TCTGTGTCGC GTCGCCGGCC TATCTCGCGC GACGCGCGGC GCCGCAGACG CTCGCCGATC TCGCGCACCA TAACTGTCTC GCGCTTGGCG CGAGTGCGAA CCAGCAGCGC GGCTGGGTGT TTCAGCAGGG TGACAAGGTC GTGACGATCA AGGTATCGGG CACGATGGAA TGCTCGGACG GAGCGGTGCT GCACGAGTGG TGCCTGGAAG GCTACGGGCT TGCATGGCGC TCGTGGTGGG AAGTGGGCGC GGACATCGCG GCGGGAAGCC TCGTCAGCGT GCTCGACAAC CATGCGGCGC CGCCCATCGG CATTCACGCC GTGTTTCCGC AGCGGCGGCA TTTGCCGTTG CGCGTGCGTC TTTTCCTCGA TTTTCTCAAG CATACCTACG GCAACCCGGC GTACTGGATC

Details for BuphA.12800.d.A1.GE32169

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMDRFKQI ETFVTVAARG SLSAAAQAEG VAPAIIGRRI DALEERLGVK LLVRTTRRIT LTYEGSAFLE DCQRIIHDMQ NAEASVSAGG VKASGHLRLS APAGFGRRHV APLVPAFTVA HPDVSISLDL SDRMVDLVNE GFDCAIRLGE LPDSSLVSLK LGENRRVCVA SPAYLARRAA PQTLADLAHH NCLALGASAN QQRGWVFQQG DKVVTIKVSG TMECSDGAVL HEWCLEGYGL AWRSWWEVGA DIAAGSLVSV LDNHAAPPIG IHAVFPQRRH LPLRVRLFLD FLKHTYGNPA YWI

Validated NT Sequence

ttattagatc cagtacgccg ggttgccgta ggtatgcttg agaaaatcga ggaaaagacg cacgcgcaac ggcaaatgcc gccgctgcgg aaacacggcg tgaatgccga tgggcggcgc cgcatggttg tcgagcacgc tgacgaggct tcccgccgcg atgtccgcgc ccacttccca ccacgagcgc catgcaagcc cgtagccttc caggcaccac tcgtgcagca ccgctccgtc cgagcattcc atcgtgcccg ataccttgat cgtcacgacc ttgtcaccct gctgaaacac ccagccgcgc tgctggttcg cactcgcgcc aagcgcgaga cagttatggt gcgcgagatc ggcgagcgtc tgcggcgccg cgcgtcgcgc gagataggcc ggcgacgcga cacagacacg ccggttctca ccgagcttca acgacaccag cgacgaatcc ggaagttcgc cgagccggat cgcgcaatcg aatccttcgt tgacgaggtc gaccatgcgg tcggacaggt cgagagaaat cgacacgtcg ggatgcgcca ccgtgaacgc aggcacgagc ggtgcgacgt gtctgcggcc gaagcccgct ggcgccgaca agcgcaaatg cccgctcgcc ttgacgccgc ccgccgacac gctcgcttcg gcgttctgca tgtcgtgaat gatccgctga cagtcttcga gaaaggcgga gccttcgtag gtcaatgtga tgcggcgcgt ggtgcgcacg agcagcttga cgccgaggcg ttcctcgagc gcgtcgatac ggcgcccgat gatggcaggc gctacgcctt ccgcctgcgc tgccgccgac aggctgccgc gcgccgcgac ggtcacgaag gtctcgatct gcttgaaacg gtccatcgaa ccaggaccct gggtctgagc ttccagggta cccatatgat ggtgatggtg atnnnccang

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDRFKQIET FVTVAARGSL SAAAQAEGVA PAIIGRRIDA LEERLGVKLL VRTTRRITLT YEGSAFLEDC QRIIHDMQNA EASVSAGGVK ASGHLRLSAP AGFGRRHVAP LVPAFTVAHP DVSISLDLSD RMVDLVNEGF DCAIRLGELP DSSLVSLKLG ENRRVCVASP AYLARRAAPQ TLADLAHHNC LALGASANQQ RGWVFQQGDK VVTIKVSGTM ECSDGAVLHE WCLEGYGLAW RSWWEVGADI AAGSLVSVLD NHAAPPIGIH AVFPQRRHLP LRVRLFLDFL KHTYGNPAYW I

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgtttc aagcagatcg agaccttcgt gaccgtcgcg gcgcgcggca gcctgtcggc ggcagcgcag gcggaaggcg tagcgcctgc catcatcggg cgccgtatcg acgcgctcga ggaacgcctc ggcgtcaagc tgctcgtgcg caccacgcgc cgcatcacat tgacctacga aggctccgcc tttctcgaag actgtcagcg gatcattcac gacatgcaga acgccgaagc gagcgtgtcg gcgggcggcg tcaaggcgag cgggcatttg cgcttgtcgg cgccagcggg cttcggccgc agacacgtcg caccgctcgt gcctgcgttc acggtggcgc atcccgacgt gtcgatttct ctcgacctgt ccgaccgcat ggtcgacctc gtcaacgaag gattcgattg cgcgatccgg ctcggcgaac ttccggattc gtcgctggtg tcgttgaagc tcggtgagaa ccggcgtgtc tgtgtcgcgt cgccggccta tctcgcgcga cgcgcggcgc cgcagacgct cgccgatctc gcgcaccata actgtctcgc gcttggcgcg agtgcgaacc agcagcgcgg ctgggtgttt cagcagggtg acaaggtcgt gacgatcaag gtatcgggca cgatggaatg ctcggacgga gcggtgctgc acgagtggtg cctggaaggc tacgggcttg catggcgctc gtggtgggaa gtgggcgcgg acatcgcggc gggaagcctc gtcagcgtgc tcgacaacca tgcggcgccg cccatcggca ttcacgccgt gtttccgcag cggcggcatt tgccgttgcg cgtgcgtctt ttcctcgatt ttctcaagca tacctacggc aacccggcgt actggatcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a