SSGCID: Target Page

BuphA.12800.c

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.c
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.c.A1.GE34732	Full length( BuphA.12800.c )		1	288

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.1322
RefSeq:	YP_001857474.1
UniProt:	B2JHT6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEVFVAVVEA GSLTAAAERF DLSSAMVGKH IRSLETQLST RLLTRTTRRQ SLTEIGRQYY EQCRRILADV KAAESLAEAM TSAPRGTLKI TVPLTYGVEL FSPAMTDYLN LYPEVSLELD LSNRIFDLVD EGFDAAVRIG RLPDSSLVAR PLKPYRMRAC ASPSYLQRMG TPATPADLVH HECLGFLHWG REGLWRLGGD AEADYQLRPG RFRANNGQAL KMAALRGFGL VLQPEALLAR EIGRGELVSV LEDYLPEGAP VHLIYPRDRQ PTPKLTTFID FVIERFGV

NT Sequence

ATGGAAGTGT TCGTGGCTGT TGTCGAAGCG GGCAGCCTCA CGGCGGCCGC GGAGCGCTTC GACCTGTCGT CAGCGATGGT CGGCAAGCAT ATCCGCTCGC TCGAAACGCA GCTTTCCACG CGGCTGCTCA CGCGCACGAC GCGCCGCCAA AGCCTGACTG AAATCGGCCG TCAGTATTAC GAGCAATGCC GGCGCATTCT CGCCGATGTG AAGGCTGCGG AGTCGCTCGC GGAAGCGATG ACCTCCGCGC CGCGCGGCAC GCTGAAGATC ACCGTGCCAC TCACCTATGG CGTCGAGCTG TTCTCGCCGG CAATGACGGA CTATCTGAAC CTCTACCCCG AAGTCAGTCT CGAACTCGAT CTGTCGAACC GCATCTTCGA TCTCGTCGAT GAAGGCTTCG ACGCGGCCGT GCGCATCGGG CGGCTGCCGG ATTCGAGCCT CGTCGCGAGA CCGCTCAAAC CTTACCGGAT GCGTGCGTGC GCGTCGCCCT CGTATCTGCA ACGCATGGGC ACGCCGGCGA CGCCTGCCGA TCTCGTTCAT CACGAATGCC TCGGCTTTCT GCATTGGGGA CGCGAAGGGC TGTGGCGACT CGGCGGCGAT GCCGAGGCGG ACTATCAGTT GCGCCCGGGC CGCTTTCGCG CGAACAACGG ACAGGCGCTG AAGATGGCAG CACTGCGCGG CTTCGGTCTC GTGCTGCAAC CGGAAGCGCT ACTCGCCCGG GAGATCGGGC GAGGTGAGCT CGTGTCCGTG CTCGAAGACT ATCTGCCGGA GGGCGCGCCT GTGCATCTGA TCTATCCGCG CGACCGCCAA CCGACGCCGA AGCTCACCAC GTTCATCGAC TTCGTGATCG AGCGCTTCGG CGTG

Details for BuphA.12800.c.A1.GE34732

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	89

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMEVFVAVVE AGSLTAAAER FDLSSAMVGK HIRSLETQLS TRLLTRTTRR QSLTEIGRQY YEQCRRILAD VKAAESLAEA MTSAPRGTLK ITVPLTYGVE LFSPAMTDYL NLYPEVSLEL DLSNRIFDLV DEGFDAAVRI GRLPDSSLVA RPLKPYRMRA CASPSYLQRM GTPATPADLV HHECLGFLHW GREGLWRLGG DAEADYQLRP GRFRANNGQA LKMAALRGXX LVLQPEALLA REIGRGELVS VLEXYLP

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaag tgttcgtggc tgttgtcgaa gcgggcagcc tcacggcggc cgcggagcgc ttcgacctgt cgtcagcgat ggtcggcaag catatccgct cgctcgaaac gcagctttcc acgcggctgc tcacgcgcac gacgcgccgc caaagcctga ctgaaatcgg ccgtcagtat tacgagcaat gccggcgcat tctcgccgat gtgaaggctg cggagtcgct cgcggaagcg atgacctccg cgccgcgcgg cacgctgaag atcaccgtgc cactcaccta tggcgtcgag ctgttctcgc cggcaatgac ggactatctg aacctctacc ccgaagtcag tctcgaactc gatctgtcga accgcatctt cgatctcgtc gatgaaggct tcgacgcggc cgtgcgcatc gggcggctgc cggattcgag cctcgtcgcg agaccgctca aaccttaccg gatgcgtgcg tgcgcgtcgc cctcgtatct gcaacgcatg ggcacgccgg cgacgcctgc cgatctcgtt catcacgaat gcctcggctt tctgcattgg ggacgcgaag ggctgtggcg actcggcggc gatgccgagg cggactatca gttgcgcccg ggccgctttc gcgcgaacaa cggacaggcg ctgaagatgg cagcactgcg cggcttnnnn ctcgtgctgc aaccggaagc gctactcgcc cgggagatcg ggcgaggtga gctcgtgtcc gtgctcgaan actatctgcc ng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaagtgttc gtggctgttg tcgaagcggg cagcctcacg gcggccgcgg agcgcttcga cctgtcgtca gcgatggtcg gcaagcatat ccgctcgctc gaaacgcagc tttccacgcg gctgctcacg cgcacgacgc gccgccaaag cctgactgaa atcggccgtc agtattacga gcaatgccgg cgcattctcg ccgatgtgaa ggctgcggag tcgctcgcgg aagcgatgac ctccgcgccg cgcggcacgc tgaagatcac cgtgccactc acctatggcg tcgagctgtt ctcgccggca atgacggact atctgaacct ctaccccgaa gtcagtctcg aactcgatct gtcgaaccgc atcttcgatc tcgtcgatga aggcttcgac gcggccgtgc gcatcgggcg gctgccggat tcgagcctcg tcgcgagacc gctcaaacct taccggatgc gtgcgtgcgc gtcgccctcg tatctgcaac gcatgggcac gccggcgacg cctgccgatc tcgttcatca cgaatgcctc ggctttctgc attggggacg cgaagggctg tggcgactcg gcggcgatgc cgaggcggac tatcagttgc gcccgggccg ctttcgcgcg aacaacggac aggcgctgaa gatggcagca ctgcgcggct tcggtctcgt gctgcaaccg gaagcgctac tcgcccggga gatcgggcga ggtgagctcg tgtccgtgct cgaagactat ctgccggagg gcgcgcctgt gcatctgatc tatccgcgcg accgccaacc gacgccgaag ctcaccacgt tcatcgactt cgtgatcgag cgcttcggcg tgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa