SSGCID: Target Page

BuphA.12800.a

Transcriptional regulator, LysR family

CENTER ID:	BuphA.12800.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.12800.a.A1.GE32170	Full length( BuphA.12800.a )		1	301

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.357
RefSeq:	YP_001856582.1
UniProt:	B2JCR7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDRFQEMQVF VRIAERQSFT RAADDLHIPR ATVTNLMKRM EARLGARLLE RTTRTVRLTH DGEAYYGRCV RLLADMEEAE GSFRDAAPKG LLRVNLQGTL ARHFVVPALP AFLSRYPGIE LHIGEDDRLV DLVREGVDCV LRAGNLQDSS MVGRRVARLE QVTVASPAYL ERHGMPCSPD ALAMHCAVNY VSSATGHALP LEFTVDERVV EVKLEAIVSV TGTDLYSGSA VAGLGLVQVP RYRVQNEIAA GSLVVVLPQF PPPPMPVTVL YPQSRQLSSR VRVFAQWLRE IFEAAGGLEV G

NT Sequence

ATGGACCGTT TTCAGGAAAT GCAGGTGTTC GTCCGCATCG CCGAGCGGCA AAGCTTCACG CGTGCCGCCG ACGATCTGCA CATCCCGCGC GCCACCGTCA CGAATCTGAT GAAGCGGATG GAAGCACGGC TCGGAGCGCG GCTGCTCGAG CGCACGACGC GCACCGTGCG CCTCACTCAC GACGGCGAAG CGTATTACGG CCGCTGCGTG CGCCTGCTCG CGGACATGGA GGAAGCCGAG GGCTCGTTTC GCGATGCCGC GCCCAAGGGC CTGCTGCGCG TGAATTTGCA AGGCACGCTC GCACGCCACT TCGTCGTGCC GGCGCTGCCC GCGTTTTTGT CACGCTATCC GGGCATCGAG CTGCATATCG GCGAGGACGA TCGGCTGGTC GATCTGGTGC GCGAGGGCGT CGATTGCGTA CTGCGCGCGG GCAATCTGCA GGATTCGTCG ATGGTCGGGC GGCGCGTCGC GCGGCTCGAA CAGGTGACCG TGGCGAGCCC GGCGTATCTC GAACGCCACG GCATGCCCTG CAGCCCCGAC GCACTCGCGA TGCATTGCGC CGTCAACTAC GTGTCGAGCG CAACGGGTCA TGCGCTGCCG CTCGAATTCA CCGTCGACGA GCGGGTGGTC GAGGTGAAGC TGGAGGCGAT CGTGTCCGTG ACGGGCACCG ATCTCTACAG CGGCTCGGCC GTCGCAGGAC TCGGGCTCGT GCAGGTGCCG CGCTATCGCG TGCAGAACGA GATTGCTGCG GGCAGCCTCG TCGTCGTGCT GCCGCAGTTT CCGCCGCCGC CGATGCCCGT CACCGTGTTG TATCCGCAAA GCCGCCAGTT GTCGTCGCGC GTGCGAGTGT TTGCGCAATG GCTGCGCGAG ATCTTCGAGG CCGCAGGCGG ACTCGAGGTC GGC

Details for BuphA.12800.a.A1.GE32170

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	35kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MXHHHHHHMG TLEAQTQGPG SMDRFQEMQV FVRIAERQSF TRAADDLHIP RATVTNLMKR MEARLGARLL ERTTRTVRLT HDGEAYYGRC VRLLADMEEA EGSFRDAAPK GLLRVNLQGT LARHFVVPAL PAFLSRYPGI ELHIGEDDRL VDLVREGVDC VLRAGNLQDS SMVGRRVARL EQVTVASPAY LERHGMPCSP DALAMHCAVN YVSSATGHAL PLEFTVDERV VEVKLEAIVS VTGTDLYSGS AVAGLGLVQV PRYRVQNEIA AGSLVVVLPQ FPPPPMPVTV LYPQSRQLSS RVRVFAQWLR EIFEAAGGLE VG

Validated NT Sequence

ttattagccg acctcgagtc cgcctgcggc ctcgaagatc tcgcgcagcc attgcgcaaa cactcgcacg cgcgacgaca actggcggct ttgcggatac aacacggtga cgggcatcgg cggcggcgga aactgcggca gcacgacgac gaggctgccc gcagcaatct cgttctgcac gcgatagcgc ggcacctgca cgagcccgag tcctgcgacg gccgagccgc tgtagagatc ggtgcccgtc acggacacga tcgcctccag cttcacctcg accacccgct cgtcgacggt gaattcgagc ggcagcgcat gacccgttgc gctcgacacg tagttgacgg cgcaatgcat cgcgagtgcg tcggggctgc agggcatgcc gtggcgttcg agatacgccg ggctcgccac ggtcacctgt tcgagccgcg cgacgcgccg cccgaccatc gacgaatcct gcagattgcc cgcgcgcagt acgcaatcga cgccctcgcg caccagatcg accagccgat cgtcctcgcc gatatgcagc tcgatgcccg gatagcgtga caaaaacgcg ggcagcgccg gcacgacgaa gtggcgtgcg agcgtgcctt gcaaattcac gcgcagcagg cccttgggcg cggcatcgcg aaacgagccc tcggcttcct ccatgtccgc gagcaggcgc acgcagcggc cgtaatacgc ttcgccgtcg tgagtgaggc gcacggtgcg cgtcgtgcgc tcgagcagcc gcgctccgag ccgtgcttcc atccgcttca tcagattcgt gacggtggcg cgcgggatgt gcagatcgtc ggcggcacgc gtgaagcttt gccgctcggc gatgcggacg aacacctgca tttcctgaaa acggtccatc gaaccaggac cctgggtctg agcttccagg gtacccatat gatggtgatg gtgatgancc atgg

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDRFQEMQV FVRIAERQSF TRAADDLHIP RATVTNLMKR MEARLGARLL ERTTRTVRLT HDGEAYYGRC VRLLADMEEA EGSFRDAAPK GLLRVNLQGT LARHFVVPAL PAFLSRYPGI ELHIGEDDRL VDLVREGVDC VLRAGNLQDS SMVGRRVARL EQVTVASPAY LERHGMPCSP DALAMHCAVN YVSSATGHAL PLEFTVDERV VEVKLEAIVS VTGTDLYSGS AVAGLGLVQV PRYRVQNEIA AGSLVVVLPQ FPPPPMPVTV LYPQSRQLSS RVRVFAQWLR EIFEAAGGLE VG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaccgtttt caggaaatgc aggtgttcgt ccgcatcgcc gagcggcaaa gcttcacgcg tgccgccgac gatctgcaca tcccgcgcgc caccgtcacg aatctgatga agcggatgga agcacggctc ggagcgcggc tgctcgagcg cacgacgcgc accgtgcgcc tcactcacga cggcgaagcg tattacggcc gctgcgtgcg cctgctcgcg gacatggagg aagccgaggg ctcgtttcgc gatgccgcgc ccaagggcct gctgcgcgtg aatttgcaag gcacgctcgc acgccacttc gtcgtgccgg cgctgcccgc gtttttgtca cgctatccgg gcatcgagct gcatatcggc gaggacgatc ggctggtcga tctggtgcgc gagggcgtcg attgcgtact gcgcgcgggc aatctgcagg attcgtcgat ggtcgggcgg cgcgtcgcgc ggctcgaaca ggtgaccgtg gcgagcccgg cgtatctcga acgccacggc atgccctgca gccccgacgc actcgcgatg cattgcgccg tcaactacgt gtcgagcgca acgggtcatg cgctgccgct cgaattcacc gtcgacgagc gggtggtcga ggtgaagctg gaggcgatcg tgtccgtgac gggcaccgat ctctacagcg gctcggccgt cgcaggactc gggctcgtgc aggtgccgcg ctatcgcgtg cagaacgaga ttgctgcggg cagcctcgtc gtcgtgctgc cgcagtttcc gccgccgccg atgcccgtca ccgtgttgta tccgcaaagc cgccagttgt cgtcgcgcgt gcgagtgttt gcgcaatggc tgcgcgagat cttcgaggcc gcaggcggac tcgaggtcgg ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa