BuphA.12245.c

Amidase, hydantoinase/carbamoylase family

CENTER ID: BuphA.12245.c
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.12245.c.B1.GE40986 Full length( BuphA.12245.c ) 1 421
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.6405
RefSeq: YP_001862191.1
UniProt: B2JW18

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMRTDQPAHD DVARRAAESI DETRLLASIE TLAAFGARPD GGVNRPALSA TDLDARRYLV ERAHALGCAV STDACANLFI RRPGIDDAPP VMTGSHIDTQ PSGGKLDGCY GVLAGLECIA ALNDAGLSTR RPLDVAIWTN EEGTRFAPGA MGSSAFVEPA RMPTYLDAAD AGGVTLRAAL ESHRRTLPEL PSRGDAPPAH AFVELHIEQG PQLENADVPL GVVTGIQGVR WYAFHCEGVA AHAGTTPMPM RRDAMTLAVA LRASLEEIAV SLGGEHTRVT FGRWSVTPNS INTIPSDVTF SVDFRHPDAH VLDTFDTLVA ACARRHGARI APLFAHTPVR FDCEVLARLD AACRALDAPA LRLTSGAFHD AMYLAQHCPT AMLFVPSRDG ISHNPAEATD ARHLVLGARA LAHTLTTLCN D
NT Sequence
ATGATGCGAA CCGATCAACC TGCACACGAT GACGTTGCAC GGCGTGCCGC GGAAAGTATC GACGAGACAC GCCTGCTCGC GTCGATCGAA ACGCTCGCCG CATTCGGCGC GCGGCCCGAC GGCGGCGTGA ACCGCCCCGC CCTCAGTGCA ACGGATCTGG ATGCGCGCCG CTATCTGGTC GAACGTGCGC ACGCGTTGGG CTGCGCCGTC TCCACCGATG CATGCGCGAA CCTCTTCATC CGCCGGCCCG GCATCGACGA TGCGCCACCC GTGATGACAG GCAGCCACAT CGACACGCAG CCTTCGGGCG GCAAGCTCGA CGGCTGCTAC GGCGTGCTCG CCGGGCTCGA ATGCATTGCC GCGCTCAACG ATGCGGGCCT AAGCACGCGC CGGCCGCTCG ACGTCGCGAT CTGGACCAAC GAAGAAGGCA CGCGCTTCGC GCCGGGCGCC ATGGGATCGA GCGCGTTTGT CGAGCCCGCG CGCATGCCCA CTTACCTCGA CGCCGCCGAC GCCGGCGGCG TCACGCTGCG CGCCGCGCTG GAGAGCCATC GGCGCACCTT GCCCGAACTG CCGTCGCGCG GCGACGCGCC GCCTGCGCAT GCGTTCGTCG AGTTGCATAT CGAGCAAGGT CCGCAACTGG AAAATGCCGA CGTGCCACTC GGCGTCGTCA CGGGAATTCA GGGCGTGCGC TGGTACGCAT TCCACTGCGA AGGCGTGGCG GCGCACGCGG GCACCACGCC GATGCCGATG CGTCGCGACG CGATGACGCT GGCCGTCGCG CTGCGGGCGT CGCTCGAAGA AATCGCCGTG TCACTGGGCG GCGAGCACAC GCGCGTGACG TTCGGGCGCT GGAGTGTGAC GCCGAATTCG ATCAACACGA TTCCATCCGA CGTGACCTTC TCCGTCGATT TCCGTCATCC CGACGCTCAT GTGCTCGACA CGTTCGACAC ACTGGTCGCC GCTTGCGCGA GGCGCCACGG CGCGCGCATC GCGCCGCTTT TCGCCCATAC GCCAGTGCGC TTCGACTGCG AGGTCCTCGC GCGGCTCGAC GCCGCCTGCC GCGCGCTCGA CGCGCCTGCG CTGCGCCTCA CGTCGGGTGC GTTTCACGAC GCAATGTATC TCGCGCAGCA CTGCCCAACG GCGATGCTGT TCGTGCCGAG CCGCGACGGC ATCAGCCACA ACCCGGCTGA AGCCACGGAC GCCCGTCACC TCGTGCTGGG CGCACGCGCG CTCGCGCACA CCCTCACCAC CCTTTGCAAC GAC
Details for BuphA.12245.c.B1.GE40986
HARVESTED ON: 1/4/2017
SEQUENCED ON: 1/5/2017
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMM RTDQPAHDDV ARRAAESIDE TRLLASIETL AAFGARPDGG VNRPALSATD LDARRYLVER AHALGCAVST DACANLFIRR PGIDDAPPVM TGSHIDTQPS GGKLDGCYGV LAGLECIAAL NDAGLSTRRP LDVAIWTNEE GTRFAPGAMG SSAFVEPARM PTYLDAADAG GVTLRAALES HRRTLPELPS RGDAPPAHAF VELHIEQGPQ LENADVPLGV VTGIQGVRWY AFHCEGVAAH AGTTPMPMRR DAMTLAVALR ASLEEIAVSL GGEHTRVTFG RWSVTPNSIN TIPSDVTFSV DFRHPDAHVL DTFDTLVAAC ARRHGARIAP LFAHTPVRFD CEVLARLDAA CRALDAPALR LTSGAFHDAM YLAQHCPTAM LFVPSRDGIS HNPAEATDAR HLVLGARALA HTLTTLCND
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgc gaaccgatca acctgcacac gatgacgttg cacggcgtgc cgcggaaagt atcgacgaga cacgcctgct cgcgtcgatc gaaacgctcg ccgcattcgg cgcgcggccc gacggcggcg tgaaccgccc cgccctcagt gcaacggatc tggatgcgcg ccgctatctg gtcgaacgtg cgcacgcgtt gggctgcgcc gtctccaccg atgcatgcgc gaacctcttc atccgccggc ccggcatcga cgatgcgcca cccgtgatga caggcagcca catcgacacg cagccttcgg gcggcaagct cgacggctgc tacggcgtgc tcgccgggct cgaatgcatt gccgcgctca acgatgcggg cctaagcacg cgccggccgc tcgacgtcgc gatctggacc aacgaagaag gcacgcgctt cgcgccgggc gccatgggat cgagcgcgtt tgtcgagccc gcgcgcatgc ccacttacct cgacgccgcc gacgccggcg gcgtcacgct gcgcgccgcg ctggagagcc atcggcgcac cttgcccgaa ctgccgtcgc gcggcgacgc gccgcctgcg catgcgttcg tcgagttgca tatcgagcaa ggtccgcaac tggaaaatgc cgacgtgcca ctcggcgtcg tcacgggaat tcagggcgtg cgctggtacg cattccactg cgaaggcgtg gcggcgcacg cgggcaccac gccgatgccg atgcgtcgcg acgcgatgac gctggccgtc gcgctgcggg cgtcgctcga agaaatcgcc gtgtcactgg gcggcgagca cacgcgcgtg acgttcgggc gctggagtgt gacgccgaat tcgatcaaca cgattccatc cgacgtgacc ttctccgtcg atttccgtca tcccgacgct catgtgctcg acacgttcga cacactggtc gccgcttgcg cgaggcgcca cggcgcgcgc atcgcgccgc ttttcgccca tacgccagtg cgcttcgact gcgaggtcct cgcgcggctc gacgccgcct gccgcgcgct cgacgcgcct gcgctgcgcc tcacgtcggg tgcgtttcac gacgcaatgt atctcgcgca gcactgccca acggcgatgc tgttcgtgcc gagccgcgac ggcatcagcc acaacccggc tgaagccacg gacgcccgtc acctcgtgct gggcgcacgc gcgctcgcgc acaccctcac caccctttgc aacgactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t