SSGCID: Target Page

BuphA.10611.a

Alcohol dehydrogenase GroES domain protein

CENTER ID:	BuphA.10611.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.10611.a.B1.GE40517	Full length( BuphA.10611.a )		1	344

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.10611.a.B1.PW38418	Full length( BuphA.10611.a )		1	344

External Resources

RESOURCE	REFERENCE ID
OrthoMCL:	OG5_126928
PATRIC ID:	fig\|391038.7.peg.7000
RefSeq:	YP_001862733.1
UniProt:	B2JSY4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKALVLEATR ELSLRDIELP QAVGPRDVKI QIHTVGVCGS DVHYYTHGRI GPFKVEAPMV LGHEASGTIV EVGADVTHLE VGDRVCMEPG IPQLDSPATM RGMYNLDPAV RFWATPPIHG CLTPFVVHPA AFTFRLPDNV SFAEGAIVEP LSIGLQAAKK AAMKPGDVAV VIGAGTIGAM TALAALAGGA SRVILADVVG AKLKHFADNT AVTTVNVSEQ SLADVVAHVT QGWGADVVFE ASGNAKVFDT LLDHACPGGC IVLVGMPPGP VALDVVAMQA KELRFESVFR YANIFPRALA LISSGMIDVK PFISRKFSFS EGVKAFEEAA AGHPADVKIQ IEMD

NT Sequence

GTGAAGGCAC TTGTACTGGA AGCCACCCGC GAACTGAGTC TGCGCGACAT CGAATTGCCG CAGGCCGTCG GCCCGCGCGA CGTCAAGATC CAGATCCACA CGGTGGGCGT GTGCGGCAGC GACGTTCACT ACTACACGCA TGGCCGCATC GGGCCGTTCA AGGTCGAAGC GCCGATGGTG CTCGGACACG AAGCATCGGG CACGATCGTC GAAGTCGGCG CGGACGTCAC CCATCTGGAA GTGGGCGACC GCGTGTGCAT GGAGCCGGGC ATTCCGCAAC TGGACTCGCC CGCGACGATG CGCGGCATGT ACAACCTCGA TCCCGCCGTG CGCTTCTGGG CGACGCCGCC CATACACGGC TGCCTGACGC CGTTTGTCGT GCATCCCGCC GCGTTCACGT TCCGCTTGCC GGATAACGTG TCGTTTGCGG AAGGCGCGAT TGTCGAGCCT TTGTCGATTG GTTTGCAGGC CGCGAAGAAG GCGGCGATGA AGCCGGGTGA TGTGGCCGTG GTCATCGGCG CTGGGACGAT CGGAGCGATG ACGGCGCTGG CGGCCCTCGC AGGCGGCGCG TCGCGCGTCA TTCTCGCGGA CGTGGTCGGC GCGAAGCTGA AGCACTTTGC CGACAACACG GCCGTTACCA CGGTCAACGT CAGCGAACAG TCGCTCGCCG ACGTGGTCGC ACACGTGACG CAAGGGTGGG GTGCCGACGT CGTGTTCGAG GCATCGGGCA ACGCAAAGGT CTTCGACACC TTGCTCGACC ACGCGTGCCC CGGTGGTTGC ATCGTGCTTG TGGGCATGCC GCCCGGACCT GTCGCGCTCG ATGTAGTGGC GATGCAGGCG AAGGAACTGC GGTTCGAATC CGTGTTCCGT TACGCCAACA TCTTCCCGCG CGCGCTTGCG CTGATCAGTT CAGGGATGAT TGATGTGAAG CCGTTCATCT CGCGCAAGTT CTCCTTTTCG GAAGGCGTGA AGGCATTCGA AGAAGCGGCT GCCGGTCATC CCGCCGACGT CAAAATCCAG ATCGAAATGG AC

Details for BuphA.10611.a.B1.GE40517

HARVESTED ON:	9/1/2016
SEQUENCED ON:	9/8/2016
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHHM VKALVLEATR ELSLRDIELP QAVGPRDVKI QIHTVGVCGS DVHYYTHGRI GPFKVEAPMV LGHEASGTIV EVGADVTHLE VGDRVCMEPG IPQLDSPATM RGMYNLDPAV RFWATPPIHG CLTPFVVHPA AFTFRLPDNV SFAEGAIVEP LSIGLQAAKK AAMKPGDVAV VIGAGTIGAM TALAALAGGA SRVILADVVG AKLKHFADNT AVTTVNVSEQ SLADVVAHVT QGWGADVVFE ASGNAKVFDT LLDHACPGGC IVLVGMPPGP VALDVVAMQA KELRFESVFR YANIFPRALA LISSGMIDVK PFISRXXSFX EGVKAXXEAA XGHPADV

Validated NT Sequence

atggctcacc accaccacca ccaccatatg gtgaaggcac ttgtactgga agccacccgc gaactgagtc tgcgcgacat cgaattgccg caggccgtcg gcccgcgcga cgtcaagatc cagatccaca cggtgggcgt gtgcggcagc gacgttcact actacacgca tggccgcatc gggccgttca aggtcgaagc gccgatggtg ctcggacacg aagcatcggg cacgatcgtc gaagtcggcg cggacgtcac ccatctggaa gtgggcgacc gcgtgtgcat ggagccgggc attccgcaac tggactcgcc cgcgacgatg cgcggcatgt acaacctcga tcccgccgtg cgcttctggg cgacgccgcc catacacggc tgcctgacgc cgtttgtcgt gcatcccgcc gcgttcacgt tccgcttgcc ggataacgtg tcgtttgcgg aaggcgcgat tgtcgagcct ttgtcgattg gtttgcaggc cgcgaagaag gcggcgatga agccgggtga tgtggccgtg gtcatcggcg ctgggacgat cggagcgatg acggcgctgg cggccctcgc aggcggcgcg tcgcgcgtca ttctcgcgga cgtggtcggc gcgaagctga agcactttgc cgacaacacg gccgttacca cggtcaacgt cagcgaacag tcgctcgccg acgtggtcgc acacgtgacg caagggtggg gtgccgacgt cgtgttcgag gcatcgggca acgcaaaggt cttcgacacc ttgctcgacc acgcgtgccc cggtggttgc atcgtgcttg tgggcatgcc gcccggacct gtcgcgctcg atgtagtggc gatgcaggcg aaggaactgc ggttcgaatc cgtgttccgt tacgccaaca tcttcccgcg cgcgcttgcg ctgatcagtt cagggatgat tgatgtgaag ccgttcatct cgcgcaannn ctccttttng gaaggcgtga aggcatnnna agaagcggct nncggtcatc ccgccgacgt ca

Expected Protein Sequence

MAHHHHHHMK ALVLEATREL SLRDIELPQA VGPRDVKIQI HTVGVCGSDV HYYTHGRIGP FKVEAPMVLG HEASGTIVEV GADVTHLEVG DRVCMEPGIP QLDSPATMRG MYNLDPAVRF WATPPIHGCL TPFVVHPAAF TFRLPDNVSF AEGAIVEPLS IGLQAAKKAA MKPGDVAVVI GAGTIGAMTA LAALAGGASR VILADVVGAK LKHFADNTAV TTVNVSEQSL ADVVAHVTQG WGADVVFEAS GNAKVFDTLL DHACPGGCIV LVGMPPGPVA LDVVAMQAKE LRFESVFRYA NIFPRALALI SSGMIDVKPF ISRKFSFSEG VKAFEEAAAG HPADVKIQIE MD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaagg cacttgtact ggaagccacc cgcgaactga gtctgcgcga catcgaattg ccgcaggccg tcggcccgcg cgacgtcaag atccagatcc acacggtggg cgtgtgcggc agcgacgttc actactacac gcatggccgc atcgggccgt tcaaggtcga agcgccgatg gtgctcggac acgaagcatc gggcacgatc gtcgaagtcg gcgcggacgt cacccatctg gaagtgggcg accgcgtgtg catggagccg ggcattccgc aactggactc gcccgcgacg atgcgcggca tgtacaacct cgatcccgcc gtgcgcttct gggcgacgcc gcccatacac ggctgcctga cgccgtttgt cgtgcatccc gccgcgttca cgttccgctt gccggataac gtgtcgtttg cggaaggcgc gattgtcgag cctttgtcga ttggtttgca ggccgcgaag aaggcggcga tgaagccggg tgatgtggcc gtggtcatcg gcgctgggac gatcggagcg atgacggcgc tggcggccct cgcaggcggc gcgtcgcgcg tcattctcgc ggacgtggtc ggcgcgaagc tgaagcactt tgccgacaac acggccgtta ccacggtcaa cgtcagcgaa cagtcgctcg ccgacgtggt cgcacacgtg acgcaagggt ggggtgccga cgtcgtgttc gaggcatcgg gcaacgcaaa ggtcttcgac accttgctcg accacgcgtg ccccggtggt tgcatcgtgc ttgtgggcat gccgcccgga cctgtcgcgc tcgatgtagt ggcgatgcag gcgaaggaac tgcggttcga atccgtgttc cgttacgcca acatcttccc gcgcgcgctt gcgctgatca gttcagggat gattgatgtg aagccgttca tctcgcgcaa gttctccttt tcggaaggcg tgaaggcatt cgaagaagcg gctgccggtc atcccgccga cgtcaaaatc cagatcgaaa tggactgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat

Details for BuphA.10611.a.B1.PW38418

PURIFICATION DATe:	1/22/2018
CONCENTRATION:	19.09mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	13
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGAAGG CACTTGTACT GGAAGCCACC CGCGAACTGA GTCTGCGCGA CATCGAATTG CCGCAGGCCG TCGGCCCGCG CGACGTCAAG ATCCAGATCC ACACGGTGGG CGTGTGCGGC AGCGACGTTC ACTACTACAC GCATGGCCGC ATCGGGCCGT TCAAGGTCGA AGCGCCGATG GTGCTCGGAC ACGAAGCATC GGGCACGATC GTCGAAGTCG GCGCGGACGT CACCCATCTG GAAGTGGGCG ACCGCGTGTG CATGGAGCCG GGCATTCCGC AACTGGACTC GCCCGCGACG ATGCGCGGCA TGTACAACCT CGATCCCGCC GTGCGCTTCT GGGCGACGCC GCCCATACAC GGCTGCCTGA CGCCGTTTGT CGTGCATCCC GCCGCGTTCA CGTTCCGCTT GCCGGATAAC GTGTCGTTTG CGGAAGGCGC GATTGTCGAG CCTTTGTCGA TTGGTTTGCA GGCCGCGAAG AAGGCGGCGA TGAAGCCGGG TGATGTGGCC GTGGTCATCG GCGCTGGGAC GATCGGAGCG ATGACGGCGC TGGCGGCCCT CGCAGGCGGC GCGTCGCGCG TCATTCTCGC GGACGTGGTC GGCGCGAAGC TGAAGCACTT TGCCGACAAC ACGGCCGTTA CCACGGTCAA CGTCAGCGAA CAGTCGCTCG CCGACGTGGT CGCACACGTG ACGCAAGGGT GGGGTGCCGA CGTCGTGTTC GAGGCATCGG GCAACGCAAA GGTCTTCGAC ACCTTGCTCG ACCACGCGTG CCCCGGTGGT TGCATCGTGC TTGTGGGCAT GCCGCCCGGA CCTGTCGCGC TCGATGTAGT GGCGATGCAG GCGAAGGAAC TGCGGTTCGA ATCCGTGTTC CGTTACGCCA ACATCTTCCC GCGCGCGCTT GCGCTGATCA GTTCAGGGAT GATTGATGTG AAGCCGTTCA TCTCGCGCAA GTTCTCCTTT TCGGAAGGCG TGAAGGCATT CGAAGAAGCG GCTGCCGGTC ATCCCGCCGA CGTCAAAATC CAGATCGAAA TGGACTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT