BuphA.10154.a
Histone deacetylase superfamily
| CENTER ID: | BuphA.10154.a |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.10154.a.B1.GE39394 | Full length( BuphA.10154.a ) | 1 | 307 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.10154.a.B1.PS37854 | Full length( BuphA.10154.a ) | 1 | 307 |
Structures
5JI5
DEPOSITED: 4/21/2016
DETERMINATION: XRay
CLONE: BuphA.10154.a.B1.GE39394
PROTEIN: BuphA.10154.a.B1.PS37854
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_126654 |
| PATRIC ID: | fig|391038.7.peg.799 |
| RefSeq: | YP_001856991.1 |
| UniProt: | B2JF16 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MATGFYSHAD CLQHEMGQWH PECPARLQAI EDQLIASRIG ELIERESAPL ADEAALLRVH TKAHVDYLRA RSPQSGYAEI DPDTSMNPHT WTAALRAAGA AVAATDAVIE GRYDNAFCSV RPPGHHAEPA RAMGFCFFNN VAIAARHALE VHKLERVAII DFDVHHGNGT EAAFSNDARV LMCSIFQHPF YPFTGADNQA PNMCNVPIAA RSKGMVVREA IDMIWLPRLD AFKPQMLFVS AGFDAHREDD LGNMALVEDD YAWITQQIRL VADKYAKGRI VSCLEGGYNL SALGRSVVAH VRALADI
NT Sequence
ATGGCAACCG GTTTCTATTC ACATGCAGAC TGTCTGCAGC ATGAGATGGG TCAATGGCAT CCCGAATGCC CGGCCCGGCT GCAGGCAATC GAAGACCAGT TGATCGCAAG CCGCATCGGC GAGCTGATCG AGCGCGAGTC CGCACCGCTT GCCGACGAAG CCGCGCTGTT GCGCGTGCAT ACGAAGGCGC ACGTCGACTA CCTCCGCGCA CGCTCGCCGC AGAGCGGCTA CGCTGAGATC GACCCCGATA CGTCGATGAA CCCGCACACG TGGACGGCCG CGTTGCGCGC AGCGGGGGCA GCCGTCGCGG CCACCGACGC CGTGATCGAA GGCCGCTACG ACAACGCGTT TTGCAGCGTG CGCCCGCCGG GCCATCATGC CGAGCCTGCA CGCGCCATGG GTTTCTGTTT CTTCAACAAT GTCGCGATCG CCGCGCGGCA TGCGCTCGAG GTGCATAAGC TGGAGCGCGT GGCGATCATC GATTTCGACG TGCATCATGG CAATGGCACG GAGGCCGCGT TTTCGAACGA CGCGCGCGTG CTGATGTGCA GCATCTTCCA GCACCCGTTC TATCCGTTCA CGGGTGCCGA CAACCAGGCA CCGAACATGT GCAACGTGCC GATCGCGGCA CGCTCGAAAG GCATGGTCGT GCGCGAAGCC ATCGACATGA TCTGGCTGCC TCGACTCGAC GCATTCAAGC CGCAGATGCT GTTCGTGTCG GCAGGCTTCG ACGCGCACCG TGAGGACGAT CTTGGCAACA TGGCGCTCGT CGAGGACGAC TATGCGTGGA TCACACAGCA GATCCGCCTG GTCGCCGACA AGTATGCGAA AGGGCGCATC GTCAGCTGCC TGGAAGGCGG CTACAACCTG TCGGCGCTCG GACGCAGCGT CGTCGCGCAC GTAAGGGCGC TCGCGGATAT C
Details for BuphA.10154.a.B1.GE39394
| HARVESTED ON: | 8/5/2015 |
| SEQUENCED ON: | 8/13/2015 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA TGFYSHADCL QHEMGQWHPE CPARLQAIED QLIASRIGEL IERESAPLAD EAALLRVHTK AHVDYLRARS PQSGYAEIDP DTSMNPHTWT AALRAAGAAV AATDAVIEGR YDNAFCSVRP PGHHAEPARA MGFCFFNNVA IAARHALEVH KLERVAIIDF DVHHGNGTEA AFSNDARVLM CSIFQHPFYP FTGADNQAPN MCNVPIAARS KGMVVREAID MIWLPRLDAF KPQMLFVSAG FDAHREDDLG NMALVEDDYA WITQQIRLVA DKYAKGRIVS CLEGGYNLSA LGRSVVAXXX GARGYLSE
Validated NT Sequence
ttactcactt agatatccgc gagcgccnnn nnnnngcgcg acgacgctgc gtccgagcgc cgacaggttg tagccgcctt ccaggcagct gacgatgcgc cctttcgcat acttgtcggc gaccaggcgg atctgctgtg tgatccacgc atagtcgtcc tcgacgagcg ccatgttgcc aagatcgtcc tcacggtgcg cgtcgaagcc tgccgacacg aacagcatct gcggcttgaa tgcgtcgagt cgaggcagcc agatcatgtc gatggcttcg cgcacgacca tgcctttcga gcgtgccgcg atcggcacgt tgcacatgtt cggtgcctgg ttgtcggcac ccgtgaacgg atagaacggg tgctggaaga tgctgcacat cagcacgcgc gcgtcgttcg aaaacgcggc ctccgtgcca ttgccatgat gcacgtcgaa atcgatgatc gccacgcgct ccagcttatg cacctcgagc gcatgccgcg cggcgatcgc gacattgttg aagaaacaga aacccatggc gcgtgcaggc tcggcatgat ggcccggcgg gcgcacgctg caaaacgcgt tgtcgtagcg gccttcgatc acggcgtcgg tggccgcgac ggctgccccc gctgcgcgca acgcggccgt ccacgtgtgc gggttcatcg acgtatcggg gtcgatctca gcgtagccgc tctgcggcga gcgtgcgcgg aggtagtcga cgtgcgcctt cgtatgcacg cgcaacagcg cggcttcgtc ggcaagcggt gcggactcgc gctcgatcag ctcgccgatg cggcttgcga tcaactggtc ttcgattgcc tgcagccggg ccgggcattc gggatgccat tgacccatct catgctgcag acagtctgca tgtgaataga aaccggttgc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMA TGFYSHADCL QHEMGQWHPE CPARLQAIED QLIASRIGEL IERESAPLAD EAALLRVHTK AHVDYLRARS PQSGYAEIDP DTSMNPHTWT AALRAAGAAV AATDAVIEGR YDNAFCSVRP PGHHAEPARA MGFCFFNNVA IAARHALEVH KLERVAIIDF DVHHGNGTEA AFSNDARVLM CSIFQHPFYP FTGADNQAPN MCNVPIAARS KGMVVREAID MIWLPRLDAF KPQMLFVSAG FDAHREDDLG NMALVEDDYA WITQQIRLVA DKYAKGRIVS CLEGGYNLSA LGRSVVAHVR ALADI
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa ccggtttcta ttcacatgca gactgtctgc agcatgagat gggtcaatgg catcccgaat gcccggcccg gctgcaggca atcgaagacc agttgatcgc aagccgcatc ggcgagctga tcgagcgcga gtccgcaccg cttgccgacg aagccgcgct gttgcgcgtg catacgaagg cgcacgtcga ctacctccgc gcacgctcgc cgcagagcgg ctacgctgag atcgaccccg atacgtcgat gaacccgcac acgtggacgg ccgcgttgcg cgcagcgggg gcagccgtcg cggccaccga cgccgtgatc gaaggccgct acgacaacgc gttttgcagc gtgcgcccgc cgggccatca tgccgagcct gcacgcgcca tgggtttctg tttcttcaac aatgtcgcga tcgccgcgcg gcatgcgctc gaggtgcata agctggagcg cgtggcgatc atcgatttcg acgtgcatca tggcaatggc acggaggccg cgttttcgaa cgacgcgcgc gtgctgatgt gcagcatctt ccagcacccg ttctatccgt tcacgggtgc cgacaaccag gcaccgaaca tgtgcaacgt gccgatcgcg gcacgctcga aaggcatggt cgtgcgcgaa gccatcgaca tgatctggct gcctcgactc gacgcattca agccgcagat gctgttcgtg tcggcaggct tcgacgcgca ccgtgaggac gatcttggca acatggcgct cgtcgaggac gactatgcgt ggatcacaca gcagatccgc ctggtcgccg acaagtatgc gaaagggcgc atcgtcagct gcctggaagg cggctacaac ctgtcggcgc tcggacgcag cgtcgtcgcg cacgtaaggg cgctcgcgga tatctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuphA.10154.a.B1.PS37854
| PURIFICATION DATe: | 3/1/2016 |
| CONCENTRATION: | 31.4mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA TGFYSHADCL QHEMGQWHPE CPARLQAIED QLIASRIGEL IERESAPLAD EAALLRVHTK AHVDYLRARS PQSGYAEIDP DTSMNPHTWT AALRAAGAAV AATDAVIEGR YDNAFCSVRP PGHHAEPARA MGFCFFNNVA IAARHALEVH KLERVAIIDF DVHHGNGTEA AFSNDARVLM CSIFQHPFYP FTGADNQAPN MCNVPIAARS KGMVVREAID MIWLPRLDAF KPQMLFVSAG FDAHREDDLG NMALVEDDYA WITQQIRLVA DKYAKGRIVS CLEGGYNLSA LGRSVVAXXX GARGYLSE
Validated NT Sequence
ttactcactt agatatccgc gagcgccnnn nnnnngcgcg acgacgctgc gtccgagcgc cgacaggttg tagccgcctt ccaggcagct gacgatgcgc cctttcgcat acttgtcggc gaccaggcgg atctgctgtg tgatccacgc atagtcgtcc tcgacgagcg ccatgttgcc aagatcgtcc tcacggtgcg cgtcgaagcc tgccgacacg aacagcatct gcggcttgaa tgcgtcgagt cgaggcagcc agatcatgtc gatggcttcg cgcacgacca tgcctttcga gcgtgccgcg atcggcacgt tgcacatgtt cggtgcctgg ttgtcggcac ccgtgaacgg atagaacggg tgctggaaga tgctgcacat cagcacgcgc gcgtcgttcg aaaacgcggc ctccgtgcca ttgccatgat gcacgtcgaa atcgatgatc gccacgcgct ccagcttatg cacctcgagc gcatgccgcg cggcgatcgc gacattgttg aagaaacaga aacccatggc gcgtgcaggc tcggcatgat ggcccggcgg gcgcacgctg caaaacgcgt tgtcgtagcg gccttcgatc acggcgtcgg tggccgcgac ggctgccccc gctgcgcgca acgcggccgt ccacgtgtgc gggttcatcg acgtatcggg gtcgatctca gcgtagccgc tctgcggcga gcgtgcgcgg aggtagtcga cgtgcgcctt cgtatgcacg cgcaacagcg cggcttcgtc ggcaagcggt gcggactcgc gctcgatcag ctcgccgatg cggcttgcga tcaactggtc ttcgattgcc tgcagccggg ccgggcattc gggatgccat tgacccatct catgctgcag acagtctgca tgtgaataga aaccggttgc catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMA TGFYSHADCL QHEMGQWHPE CPARLQAIED QLIASRIGEL IERESAPLAD EAALLRVHTK AHVDYLRARS PQSGYAEIDP DTSMNPHTWT AALRAAGAAV AATDAVIEGR YDNAFCSVRP PGHHAEPARA MGFCFFNNVA IAARHALEVH KLERVAIIDF DVHHGNGTEA AFSNDARVLM CSIFQHPFYP FTGADNQAPN MCNVPIAARS KGMVVREAID MIWLPRLDAF KPQMLFVSAG FDAHREDDLG NMALVEDDYA WITQQIRLVA DKYAKGRIVS CLEGGYNLSA LGRSVVAHVR ALADI
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCAA CCGGTTTCTA TTCACATGCA GACTGTCTGC AGCATGAGAT GGGTCAATGG CATCCCGAAT GCCCGGCCCG GCTGCAGGCA ATCGAAGACC AGTTGATCGC AAGCCGCATC GGCGAGCTGA TCGAGCGCGA GTCCGCACCG CTTGCCGACG AAGCCGCGCT GTTGCGCGTG CATACGAAGG CGCACGTCGA CTACCTCCGC GCACGCTCGC CGCAGAGCGG CTACGCTGAG ATCGACCCCG ATACGTCGAT GAACCCGCAC ACGTGGACGG CCGCGTTGCG CGCAGCGGGG GCAGCCGTCG CGGCCACCGA CGCCGTGATC GAAGGCCGCT ACGACAACGC GTTTTGCAGC GTGCGCCCGC CGGGCCATCA TGCCGAGCCT GCACGCGCCA TGGGTTTCTG TTTCTTCAAC AATGTCGCGA TCGCCGCGCG GCATGCGCTC GAGGTGCATA AGCTGGAGCG CGTGGCGATC ATCGATTTCG ACGTGCATCA TGGCAATGGC ACGGAGGCCG CGTTTTCGAA CGACGCGCGC GTGCTGATGT GCAGCATCTT CCAGCACCCG TTCTATCCGT TCACGGGTGC CGACAACCAG GCACCGAACA TGTGCAACGT GCCGATCGCG GCACGCTCGA AAGGCATGGT CGTGCGCGAA GCCATCGACA TGATCTGGCT GCCTCGACTC GACGCATTCA AGCCGCAGAT GCTGTTCGTG TCGGCAGGCT TCGACGCGCA CCGTGAGGAC GATCTTGGCA ACATGGCGCT CGTCGAGGAC GACTATGCGT GGATCACACA GCAGATCCGC CTGGTCGCCG ACAAGTATGC GAAAGGGCGC ATCGTCAGCT GCCTGGAAGG CGGCTACAAC CTGTCGGCGC TCGGACGCAG CGTCGTCGCG CACGTAAGGG CGCTCGCGGA TATCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT