BuphA.10100.c

2-alkenal reductase

CENTER ID: BuphA.10100.c
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.10100.c.B1.GE40515 Full length( BuphA.10100.c ) 1 341
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3863
RefSeq: YP_001859840.1
UniProt: B2JMK0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGSRPRFIDD LSGARPAAAS PGSPGDDALL DAYSRTVIDA LERVRAAVVF ITVERRVPGA RERHAPAGTG SGFLFTPDGY LLTNSHVVHG ATHIGVQLAD GTKFDADLVG DDPHSDLAVL RIGSREPLPH VALGESGKLR VGQIAIAVGN PLGLEQTVTA GVVSALGRSL RSNSGRMIYD VIQTDAALNP GNSGGPLINS AGQVIGVNTA IIPGAQSISF ATAIDTAKWV IMQIFAHGRV RRAYIGVAGT TTALPRRVQR YFELDSVSGV RVIEIVKGSP AAVGGLRVDD TIVAVDGEVV EGVDALQRAL DGSKIEREVV VSVLRGAKRV ELVVLPAEQV V
NT Sequence
ATGGGAAGCC GTCCCCGTTT TATCGACGAT CTGTCGGGCG CGCGGCCAGC CGCCGCTTCG CCCGGATCGC CTGGCGACGA CGCACTCCTC GACGCCTATT CGCGCACCGT CATCGATGCG CTCGAACGGG TGCGGGCGGC CGTCGTCTTC ATCACCGTCG AGCGCCGCGT GCCGGGCGCG CGGGAGCGCC ACGCACCTGC GGGCACCGGC TCGGGGTTCC TCTTCACGCC CGACGGTTAT CTTCTGACCA ACAGCCACGT CGTGCACGGC GCGACGCATA TCGGCGTGCA ACTCGCCGAC GGCACGAAGT TCGACGCCGA TCTGGTCGGT GACGATCCGC ATAGCGACCT TGCCGTGCTG CGCATCGGTT CGCGGGAGCC GCTGCCGCAT GTCGCGCTCG GCGAGTCGGG CAAGCTGCGC GTCGGCCAGA TTGCGATTGC CGTCGGCAAT CCGCTCGGGC TCGAGCAGAC GGTCACGGCG GGCGTGGTGT CCGCGCTGGG CCGCTCGCTG CGTTCGAATT CGGGCCGCAT GATCTATGAC GTGATCCAGA CGGATGCCGC GCTGAATCCG GGCAATTCCG GCGGCCCGCT GATCAATTCG GCGGGGCAGG TGATCGGCGT GAATACGGCG ATCATTCCGG GTGCGCAGTC GATCAGCTTC GCCACGGCCA TCGATACCGC GAAGTGGGTC ATCATGCAGA TCTTCGCGCA TGGGCGCGTG CGGCGCGCCT ACATCGGCGT AGCCGGCACG ACGACGGCGC TGCCGCGCCG TGTGCAGCGT TATTTCGAAC TCGATTCGGT AAGTGGCGTG CGAGTGATCG AGATCGTGAA AGGGTCGCCG GCGGCTGTCG GAGGTTTGCG TGTGGATGAC ACCATTGTCG CTGTCGATGG CGAGGTCGTT GAAGGTGTCG ACGCTCTGCA GCGTGCGCTC GATGGGTCGA AGATCGAGCG TGAAGTCGTC GTTAGTGTTT TGCGCGGTGC GAAGCGGGTT GAACTGGTGG TTTTGCCTGC TGAGCAGGTT GTA
Details for BuphA.10100.c.B1.GE40515
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 37kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHXG SRPRFIDDLS GARPAAASPG SPGDDALLDA YSRTVIDALE RVRAAVVFIT VERRVPGARE RHAPAGTGSG FLFTPDGYLL TNSHVVHGAT HIGVQLADGT KFDADLVGDD PHSDLAVLRI GSREPLPHVA LGESGKLRVG QIAIAVGNPL GLEQTVTAGV VSALGRSLRS NSGRMIYDVI QTDAALNPGN SGGPLINSAG QVIGVNTAII PGAQSISFAT AIDTAKWVIM QIFAHGRVRR AYIGVAGTTT ALPRRVQRYF ELDSVSGVRV IEIVKGSPAA VGGLRVDDTI VAVDGEVVEG VDALQRALDG SKIEREVVVS VLRGAKRVEL
Validated NT Sequence
atggctcacc accaccacca ccatanggga agccgtcccc gttttatcga cgatctgtcg ggcgcgcggc cagccgccgc ttcgcccgga tcgcctggcg acgacgcact cctcgacgcc tattcgcgca ccgtcatcga tgcgctcgaa cgggtgcggg cggccgtcgt cttcatcacc gtcgagcgcc gcgtgccggg cgcgcgggag cgccacgcac ctgcgggcac cggctcgggg ttcctcttca cgcccgacgg ttatcttctg accaacagcc acgtcgtgca cggcgcgacg catatcggcg tgcaactcgc cgacggcacg aagttcgacg ccgatctggt cggtgacgat ccgcatagcg accttgccgt gctgcgcatc ggttcgcggg agccgctgcc gcatgtcgcg ctcggcgagt cgggcaagct gcgcgtcggc cagattgcga ttgccgtcgg caatccgctc gggctcgagc agacggtcac ggcgggcgtg gtgtccgcgc tgggccgctc gctgcgttcg aattcgggcc gcatgatcta tgacgtgatc cagacggatg ccgcgctgaa tccgggcaat tccggcggcc cgctgatcaa ttcggcgggg caggtgatcg gcgtgaatac ggcgatcatt ccgggtgcgc agtcgatcag cttcgccacg gccatcgata ccgcgaagtg ggtcatcatg cagatcttcg cgcatgggcg cgtgcggcgc gcctacatcg gcgtagccgg cacgacgacg gcgctgccgc gccgtgtgca gcgttatttc gaactcgatt cggtaagtgg cgtgcgagtg atcgagatcg tgaaagggtc gccggcggct gtcggaggtt tgcgtgtgga tgacaccatt gtcgctgtcg atggcgaggt cgttgaaggt gtcgacgctc tgcagcgtgc gctcgatggg tcgaagatcg agcgtgaagt cgtcgttagt gttttgcgcg gtgcgaagcg ggttgaactg gt
Expected Protein Sequence
MAHHHHHHMG SRPRFIDDLS GARPAAASPG SPGDDALLDA YSRTVIDALE RVRAAVVFIT VERRVPGARE RHAPAGTGSG FLFTPDGYLL TNSHVVHGAT HIGVQLADGT KFDADLVGDD PHSDLAVLRI GSREPLPHVA LGESGKLRVG QIAIAVGNPL GLEQTVTAGV VSALGRSLRS NSGRMIYDVI QTDAALNPGN SGGPLINSAG QVIGVNTAII PGAQSISFAT AIDTAKWVIM QIFAHGRVRR AYIGVAGTTT ALPRRVQRYF ELDSVSGVRV IEIVKGSPAA VGGLRVDDTI VAVDGEVVEG VDALQRALDG SKIEREVVVS VLRGAKRVEL VVLPAEQVV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggaa gccgtccccg ttttatcgac gatctgtcgg gcgcgcggcc agccgccgct tcgcccggat cgcctggcga cgacgcactc ctcgacgcct attcgcgcac cgtcatcgat gcgctcgaac gggtgcgggc ggccgtcgtc ttcatcaccg tcgagcgccg cgtgccgggc gcgcgggagc gccacgcacc tgcgggcacc ggctcggggt tcctcttcac gcccgacggt tatcttctga ccaacagcca cgtcgtgcac ggcgcgacgc atatcggcgt gcaactcgcc gacggcacga agttcgacgc cgatctggtc ggtgacgatc cgcatagcga ccttgccgtg ctgcgcatcg gttcgcggga gccgctgccg catgtcgcgc tcggcgagtc gggcaagctg cgcgtcggcc agattgcgat tgccgtcggc aatccgctcg ggctcgagca gacggtcacg gcgggcgtgg tgtccgcgct gggccgctcg ctgcgttcga attcgggccg catgatctat gacgtgatcc agacggatgc cgcgctgaat ccgggcaatt ccggcggccc gctgatcaat tcggcggggc aggtgatcgg cgtgaatacg gcgatcattc cgggtgcgca gtcgatcagc ttcgccacgg ccatcgatac cgcgaagtgg gtcatcatgc agatcttcgc gcatgggcgc gtgcggcgcg cctacatcgg cgtagccggc acgacgacgg cgctgccgcg ccgtgtgcag cgttatttcg aactcgattc ggtaagtggc gtgcgagtga tcgagatcgt gaaagggtcg ccggcggctg tcggaggttt gcgtgtggat gacaccattg tcgctgtcga tggcgaggtc gttgaaggtg tcgacgctct gcagcgtgcg ctcgatgggt cgaagatcga gcgtgaagtc gtcgttagtg ttttgcgcgg tgcgaagcgg gttgaactgg tggttttgcc tgctgagcag gttgtatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t