BuphA.10100.a

Protease Do

CENTER ID: BuphA.10100.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.10100.a.B2.GE40513 Mature protein( BuphA.10100.a ) 26 500
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.493
RefSeq: YP_001856709.1
UniProt: B2JDE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKANMLTRSA VAAAVVIALS AGYVAGHRNV PAPAVITPAV AAMMPAEAAA KTGIPDFSGL VETYGPAVVN ISAKHVVKQT AMRGGNNSGQ LPIDPSDPFY QFYRHFFGQM PGGPNGGGDD GGDRPSASLG SGFIISSDGY VLTNAHVVDG ANVVTVKLTD KREYRAKVVG ADKQSDVAVL KIDAKDLPTV KIGDPRQSKV GQWVVAIGSP YGFDNTVTSG IISAKSRSLP DENYTPFIQT DVPVNPGNSG GPLFNLQGEV IGINSMIYSQ TGGFQGLSFA IPINEAIKVK DDLVKTGHVS RGRLGVAVQS VNQTLADSFG MKKPQGALVS SVDPGGPAAK AGLQPGDVIL SVDGVDVVDS AALPSQIAGI RPGKQVDVQV WRDKSTKDMK VTIGSLSDVK AAANDDGGPA QMQGRLGVAV RPLTPQEKSG ASVSHGLLVQ DASGAAASAG IQPGDVILAV NGRAVSSVDQ LKQAVSGAGN SIALLIQRDN SQIFVPVDLG
NT Sequence
ATGAAAGCAA ATATGCTGAC CCGTAGCGCC GTCGCGGCGG CCGTCGTCAT CGCTTTGTCG GCGGGTTATG TGGCCGGCCA CCGCAATGTC CCGGCGCCCG CGGTGATCAC CCCGGCCGTG GCCGCGATGA TGCCCGCCGA AGCCGCGGCC AAAACGGGTA TCCCCGATTT CTCGGGTCTC GTCGAAACTT ATGGTCCCGC TGTCGTCAAC ATCAGCGCGA AGCACGTCGT CAAGCAGACG GCGATGCGCG GCGGCAACAA CAGCGGCCAG TTGCCCATCG ACCCGAGCGA TCCGTTTTAT CAGTTCTACC GCCACTTTTT TGGCCAGATG CCGGGTGGCC CCAATGGCGG CGGTGACGAC GGCGGCGACC GTCCGAGCGC GAGCCTCGGC TCCGGCTTCA TTATCAGCAG CGATGGTTAT GTGCTGACCA ATGCGCACGT CGTCGACGGT GCGAACGTCG TCACCGTGAA ACTCACCGAC AAGCGCGAGT ACAGGGCGAA AGTGGTCGGC GCCGACAAGC AGTCGGATGT CGCAGTCCTG AAGATCGACG CGAAGGATCT GCCGACCGTG AAGATCGGCG ATCCGCGCCA GAGCAAGGTC GGCCAGTGGG TCGTCGCGAT CGGCTCGCCC TACGGCTTCG ACAACACGGT GACGTCGGGC ATCATCAGCG CGAAGTCGCG CTCGCTGCCG GACGAGAACT ACACGCCGTT CATCCAGACG GACGTGCCCG TGAATCCGGG TAACTCGGGC GGCCCGCTGT TCAATCTGCA AGGCGAAGTG ATCGGCATCA ATTCGATGAT CTATTCGCAG ACGGGCGGCT TCCAGGGCCT TTCGTTCGCT ATCCCGATCA ATGAGGCGAT CAAGGTCAAG GACGATCTCG TAAAGACCGG CCACGTGAGC CGCGGCCGTC TCGGCGTCGC GGTGCAGAGT GTGAACCAGA CGCTCGCGGA TTCATTCGGC ATGAAGAAGC CGCAAGGCGC GCTGGTCAGC TCCGTCGATC CGGGCGGCCC GGCGGCGAAA GCAGGCCTGC AGCCCGGCGA CGTGATCCTG TCGGTGGATG GCGTCGACGT GGTGGATTCG GCGGCGCTGC CTTCGCAGAT CGCGGGCATC AGGCCGGGCA AGCAAGTCGA CGTGCAGGTG TGGCGCGACA AGTCGACGAA AGATATGAAA GTGACGATCG GCTCGTTGTC CGACGTGAAA GCGGCCGCGA ACGACGACGG TGGTCCCGCG CAGATGCAAG GCCGCCTCGG CGTCGCCGTG CGTCCGCTGA CGCCGCAGGA GAAGAGCGGC GCGTCTGTGT CGCACGGTCT GTTGGTGCAG GACGCGAGCG GCGCGGCGGC GAGCGCGGGT ATCCAGCCCG GGGACGTGAT TCTGGCCGTC AACGGACGCG CGGTGTCGAG CGTCGATCAG CTGAAGCAGG CGGTATCTGG AGCAGGTAAC AGCATCGCGC TCCTGATCCA GCGCGACAAC TCGCAGATCT TCGTGCCCGT CGATCTGGGC
Details for BuphA.10100.a.B2.GE40513
HARVESTED ON: 9/1/2016
SEQUENCED ON: 9/8/2016
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHHM GHRNVPAPAV ITPAVAAMMP AEAAAKTGIP DFSGLVETYG PAVVNISAKH VVKQTAMRGG NNSGQLPIDP SDPFYQFYRH FFGQMPGGPN GGGDDGGDRP SASLGSGFII SSDGYVLTNA HVVDGANVVT VKLTDKREYR AKVVGADKQS DVAVLKIDAK DLPTVKIGDP RQSKVGQWVV AIGSPYGFDN TVTSGIISAK SRSLPDENYT PFIQTDVPVN PGNSGGPLFN LQGEVIGINS MIYSQTGGFQ GLSFAIPINE AIKVKDDLVK TGHVSRGRLG VAVQSVNQTL ADSFGMKKPQ GALVSSVDPG GPAAKAGLQP GDVILSVDGV DVVDSAALPS QIAGIRPGKQ VDVQVWRDKS TKDMKVTIGS LSDVKAAAND DGGPAQMQGR LGVAVRPLTP QEKSGASVSH GLLVQDASGA AASAGIQPGD VILAVNGRAV SSVDQLKQAV SGAGNSIALL IQRDNS
Validated NT Sequence
atggctcacc accaccacca ccaccatatg ggccaccgca atgtcccggc gcccgcggtg atcaccccgg ccgtggccgc gatgatgccc gccgaagccg cggccaaaac gggtatcccc gatttctcgg gtctcgtcga aacttatggt cccgctgtcg tcaacatcag cgcgaagcac gtcgtcaagc agacggcgat gcgcggcggc aacaacagcg gccagttgcc catcgacccg agcgatccgt tttatcagtt ctaccgccac ttttttggcc agatgccggg tggccccaat ggcggcggtg acgacggcgg cgaccgtccg agcgcgagcc tcggctccgg cttcattatc agcagcgatg gttatgtgct gaccaatgcg cacgtcgtcg acggtgcgaa cgtcgtcacc gtgaaactca ccgacaagcg cgagtacagg gcgaaagtgg tcggcgccga caagcagtcg gatgtcgcag tcctgaagat cgacgcgaag gatctgccga ccgtgaagat cggcgatccg cgccagagca aggtcggcca gtgggtcgtc gcgatcggct cgccctacgg cttcgacaac acggtgacgt cgggcatcat cagcgcgaag tcgcgctcgc tgccggacga gaactacacg ccgttcatcc agacggacgt gcccgtgaat ccgggtaact cgggcggccc gctgttcaat ctgcaaggcg aagtgatcgg catcaattcg atgatctatt cgcagacggg cggcttccag ggcctttcgt tcgctatccc gatcaatgag gcgatcaagg tcaaggacga tctcgtaaag accggccacg tgagccgcgg ccgtctcggc gtcgcggtgc agagtgtgaa ccagacgctc gcggattcat tcggcatgaa gaagccgcaa ggcgcgctgg tcagctccgt cgatccgggc ggcccggcgg cgaaagcagg cctgcagccc ggcgacgtga tcctgtcggt ggatggcgtc gacgtggtgg attcggcggc gctgccttcg cagatcgcgg gcatcaggcc gggcaagcaa gtcgacgtgc aggtgtggcg cgacaagtcg acgaaagata tgaaagtgac gatcggctcg ttgtccgacg tgaaagcggc cgcgaacgac gacggtggtc ccgcgcagat gcaaggccgc ctcggcgtcg ccgtgcgtcc gctgacgccg caggagaaga gcggcgcgtc tgtgtcgcac ggtctgttgg tgcaggacgc gagcggcgcg gcggcgagcg cgggtatcca gcccggggac gtgattctgg ccgtcaacgg acgcgcggtg tcgagcgtcg atcagctgaa gcaggcggta tctggagcag gtaacagcat cgcgctcctg atccagcgcg acaactcgc
Expected Protein Sequence
MAHHHHHHGH RNVPAPAVIT PAVAAMMPAE AAAKTGIPDF SGLVETYGPA VVNISAKHVV KQTAMRGGNN SGQLPIDPSD PFYQFYRHFF GQMPGGPNGG GDDGGDRPSA SLGSGFIISS DGYVLTNAHV VDGANVVTVK LTDKREYRAK VVGADKQSDV AVLKIDAKDL PTVKIGDPRQ SKVGQWVVAI GSPYGFDNTV TSGIISAKSR SLPDENYTPF IQTDVPVNPG NSGGPLFNLQ GEVIGINSMI YSQTGGFQGL SFAIPINEAI KVKDDLVKTG HVSRGRLGVA VQSVNQTLAD SFGMKKPQGA LVSSVDPGGP AAKAGLQPGD VILSVDGVDV VDSAALPSQI AGIRPGKQVD VQVWRDKSTK DMKVTIGSLS DVKAAANDDG GPAQMQGRLG VAVRPLTPQE KSGASVSHGL LVQDASGAAA SAGIQPGDVI LAVNGRAVSS VDQLKQAVSG AGNSIALLIQ RDNSQIFVPV DLG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catggccacc gcaatgtccc ggcgcccgcg gtgatcaccc cggccgtggc cgcgatgatg cccgccgaag ccgcggccaa aacgggtatc cccgatttct cgggtctcgt cgaaacttat ggtcccgctg tcgtcaacat cagcgcgaag cacgtcgtca agcagacggc gatgcgcggc ggcaacaaca gcggccagtt gcccatcgac ccgagcgatc cgttttatca gttctaccgc cacttttttg gccagatgcc gggtggcccc aatggcggcg gtgacgacgg cggcgaccgt ccgagcgcga gcctcggctc cggcttcatt atcagcagcg atggttatgt gctgaccaat gcgcacgtcg tcgacggtgc gaacgtcgtc accgtgaaac tcaccgacaa gcgcgagtac agggcgaaag tggtcggcgc cgacaagcag tcggatgtcg cagtcctgaa gatcgacgcg aaggatctgc cgaccgtgaa gatcggcgat ccgcgccaga gcaaggtcgg ccagtgggtc gtcgcgatcg gctcgcccta cggcttcgac aacacggtga cgtcgggcat catcagcgcg aagtcgcgct cgctgccgga cgagaactac acgccgttca tccagacgga cgtgcccgtg aatccgggta actcgggcgg cccgctgttc aatctgcaag gcgaagtgat cggcatcaat tcgatgatct attcgcagac gggcggcttc cagggccttt cgttcgctat cccgatcaat gaggcgatca aggtcaagga cgatctcgta aagaccggcc acgtgagccg cggccgtctc ggcgtcgcgg tgcagagtgt gaaccagacg ctcgcggatt cattcggcat gaagaagccg caaggcgcgc tggtcagctc cgtcgatccg ggcggcccgg cggcgaaagc aggcctgcag cccggcgacg tgatcctgtc ggtggatggc gtcgacgtgg tggattcggc ggcgctgcct tcgcagatcg cgggcatcag gccgggcaag caagtcgacg tgcaggtgtg gcgcgacaag tcgacgaaag atatgaaagt gacgatcggc tcgttgtccg acgtgaaagc ggccgcgaac gacgacggtg gtcccgcgca gatgcaaggc cgcctcggcg tcgccgtgcg tccgctgacg ccgcaggaga agagcggcgc gtctgtgtcg cacggtctgt tggtgcagga cgcgagcggc gcggcggcga gcgcgggtat ccagcccggg gacgtgattc tggccgtcaa cggacgcgcg gtgtcgagcg tcgatcagct gaagcaggcg gtatctggag caggtaacag catcgcgctc ctgatccagc gcgacaactc gcagatcttc gtgcccgtcg atctgggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat