BuphA.01673.r

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuphA.01673.r
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01673.r.A2.GE34738 Mature protein( BuphA.01673.r ) 33 486
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.7515
RefSeq: YP_001863256.1
UniProt: B2JUN9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFLVRGSLVR KAIAVAVASA LGACSTLPHY SQPQSAVPDH FASTPQPAAG WTVAAPADGE ARGPWWTLFN DDELNHLEAQ VDVSNQTVRK AVAQLEAARS MVDYQRAGYA PVVTAGASAQ RYRTSQNVVH KGLAGHTVPD FSTGLAASWE PDLFGRVKNA TVNARDNAQA SEADLQSVRL SVATDLAIDY FDLRSLDRQK KLLDDTVTAY AAALRIVQQQ LKDGAIDASA VAQAQTQLES TRTQDSDIDV QRAQLEHAIA TLVGQPASSF SLPPKVETVA LPQIPAGVPS QLLERRPDIA AAERRVAAAN AQIGEARAAF YPNLTLSATA GLESSFFAPW LTASSLFWSL GSQIAGTLFD GGRRAAALNN ASAQYDGTVA DYRQTVLVAF QQVEDDLSSL NTLANEADSQ QRATAAADLS LKLTTNRYQA GAVDYLNVVT AQTIALSNER AAEQIDARRI DASVQLLKAL GGGWDRNALT DANATD
NT Sequence
ATGTTCCTTG TCCGCGGATC GCTGGTTCGC AAGGCAATTG CCGTAGCTGT CGCAAGCGCG TTGGGCGCGT GTTCGACACT GCCGCACTAC TCGCAGCCGC AATCGGCTGT GCCCGATCAC TTCGCGAGCA CGCCGCAACC CGCAGCCGGC TGGACAGTCG CCGCGCCCGC CGATGGAGAG GCGCGCGGCC CGTGGTGGAC GCTCTTCAAC GACGACGAAC TGAATCATCT GGAAGCGCAG GTCGACGTGT CGAACCAGAC CGTGCGCAAG GCCGTCGCGC AACTGGAAGC GGCGCGCTCG ATGGTCGACT ATCAGCGCGC AGGTTATGCG CCCGTCGTCA CCGCGGGCGC CTCGGCGCAA CGCTATCGCA CGTCGCAGAA TGTGGTGCAC AAAGGGCTGG CGGGCCATAC TGTGCCCGAT TTTTCGACGG GACTCGCGGC GAGCTGGGAA CCCGATCTGT TCGGTCGGGT AAAGAACGCG ACCGTCAATG CGCGCGACAA TGCGCAGGCC AGCGAAGCCG ATCTGCAATC GGTGCGTCTT TCCGTTGCCA CCGATCTCGC AATCGACTAT TTCGATTTGC GCTCGCTCGA CCGGCAAAAG AAGCTGCTCG ACGATACGGT CACCGCCTAC GCCGCTGCAC TGCGGATCGT GCAACAGCAA CTGAAGGACG GCGCGATCGA TGCGTCTGCC GTCGCGCAGG CGCAGACGCA GCTGGAAAGC ACGCGCACGC AAGATAGCGA CATCGATGTG CAGCGCGCGC AGCTCGAGCA TGCGATCGCC ACGCTGGTCG GTCAGCCCGC ATCGTCTTTC TCGCTACCGC CGAAGGTGGA GACCGTCGCG CTGCCGCAGA TTCCCGCGGG CGTGCCGTCG CAATTGCTGG AGCGCCGTCC CGACATCGCC GCCGCCGAAC GGCGCGTCGC CGCCGCAAAC GCGCAGATCG GCGAAGCGCG CGCGGCGTTC TATCCGAACC TGACGCTGTC GGCGACGGCG GGGCTCGAAA GCTCGTTCTT CGCGCCGTGG CTCACGGCAT CGAGCCTGTT CTGGTCGCTC GGCTCACAGA TCGCGGGTAC GCTGTTCGAC GGCGGCCGGC GCGCGGCAGC GCTGAACAAT GCAAGCGCGC AATACGACGG CACGGTCGCC GATTACCGGC AAACGGTGCT GGTGGCCTTC CAGCAGGTCG AGGACGATCT GTCGTCGCTC AATACGCTCG CGAACGAAGC CGACAGCCAG CAACGCGCGA CAGCGGCCGC CGACCTGTCG CTCAAGCTGA CGACCAATCG CTATCAGGCG GGTGCCGTCG ACTATCTGAA CGTGGTGACA GCACAAACCA TCGCGCTGTC CAACGAGCGC GCGGCGGAAC AGATCGACGC GCGCCGTATC GATGCAAGCG TGCAATTGCT GAAAGCGCTC GGCGGCGGCT GGGACCGCAA TGCGTTGACG GATGCAAACG CAACGGAT
Details for BuphA.01673.r.A2.GE34738
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/12/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPQSAVPDH FASTPQPAAG WTVAAPADGE ARGPWWTLFN DDELNHLEAQ VDVSNQTVRK AVAQLEAARS MVDYQRAGYA PVVTAGASAQ RYRTSQNVVH KGLAGHTVPD FSTGLAASWE PDLFGRVKNA TVNARDNAQA SEADLQSVRL SVATDLAIDY FDLRSLDRQK KLLDDTVTAY AAALRIVQQQ LKDGAIDASA VAQAQTQLES TRTQDSDIDV QRAQLEHAIA TLVGQPASSF SLPPXVETVA LPQIPAGVPS QLLERRPDIA AAERRVAAAN AQIGEARAAF YPNLTLXRXX ARXLVLRXXX XXSXXXXXSX XXSXXTXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
annnnnnntt nnttancttt aagaaggaga nataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgccgcaatc ggctgtgccc gatcacttcg cgagcacgcc gcaacccgca gccggctgga cagtcgccgc gcccgccgat ggagaggcgc gcggcccgtg gtggacgctc ttcaacgacg acgaactgaa tcatctggaa gcgcaggtcg acgtgtcgaa ccagaccgtg cgcaaggccg tcgcgcaact ggaagcggcg cgctcgatgg tcgactatca gcgcgcaggt tatgcgcccg tcgtcaccgc gggcgcctcg gcgcaacgct atcgcacgtc gcagaatgtg gtgcacaaag ggctggcggg ccatactgtg cccgattttt cgacgggact cgcggcgagc tgggaacccg atctgttcgg tcgggtaaag aacgcgaccg tcaatgcgcg cgacaatgcg caggccagcg aagccgatct gcaatcggtg cgtctttccg ttgccaccga tctcgcaatc gactatttcg atttgcgctc gctcgaccgg caaaagaagc tgctcgacga tacggtcacc gcctacgccg ctgcactgcg gatcgtgcaa cagcaactga aggacggcgc gatcgatgcg tctgccgtcg cgcaggcgca gacgcagctg gaaagcacgc gcacgcaaga tagcgacatc gatgtgcagc gcgcgcagct cgagcatgcg atcgccacgc tggtcggtca gcccgcatcg tctttctcgc taccgccgan ngtggagacc gtcgcgctgc cgcagattcc cgcgggcgtg ccgtcgcaat tgctggagcg ccgtcccgac atcgccgccg ccgaacggcg cgtcgccgcc gcaaacgcnc agatcggcga agcgcgcgcg gcgttctatc cnaacctgac gctgtngcga ngnnnggctc gaangctcgt ncttcgcnnc gnnnnnnngg natcannctg ntntnnncnc tcngntnnnn natcgcnggn nacncnnntc gnnnnnnnnn nnnngnannn ctnnnnnnna nnnnnnnnnn ngnnnnannn nnnnnnnnnn nnnngnnnnn nnnnnnnnnn nnnnnnnann nnnnnnnnna nnnnnnnnnn nnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPQSAVPDH FASTPQPAAG WTVAAPADGE ARGPWWTLFN DDELNHLEAQ VDVSNQTVRK AVAQLEAARS MVDYQRAGYA PVVTAGASAQ RYRTSQNVVH KGLAGHTVPD FSTGLAASWE PDLFGRVKNA TVNARDNAQA SEADLQSVRL SVATDLAIDY FDLRSLDRQK KLLDDTVTAY AAALRIVQQQ LKDGAIDASA VAQAQTQLES TRTQDSDIDV QRAQLEHAIA TLVGQPASSF SLPPKVETVA LPQIPAGVPS QLLERRPDIA AAERRVAAAN AQIGEARAAF YPNLTLSATA GLESSFFAPW LTASSLFWSL GSQIAGTLFD GGRRAAALNN ASAQYDGTVA DYRQTVLVAF QQVEDDLSSL NTLANEADSQ QRATAAADLS LKLTTNRYQA GAVDYLNVVT AQTIALSNER AAEQIDARRI DASVQLLKAL GGGWDRNALT DANATD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgcaatcg gctgtgcccg atcacttcgc gagcacgccg caacccgcag ccggctggac agtcgccgcg cccgccgatg gagaggcgcg cggcccgtgg tggacgctct tcaacgacga cgaactgaat catctggaag cgcaggtcga cgtgtcgaac cagaccgtgc gcaaggccgt cgcgcaactg gaagcggcgc gctcgatggt cgactatcag cgcgcaggtt atgcgcccgt cgtcaccgcg ggcgcctcgg cgcaacgcta tcgcacgtcg cagaatgtgg tgcacaaagg gctggcgggc catactgtgc ccgatttttc gacgggactc gcggcgagct gggaacccga tctgttcggt cgggtaaaga acgcgaccgt caatgcgcgc gacaatgcgc aggccagcga agccgatctg caatcggtgc gtctttccgt tgccaccgat ctcgcaatcg actatttcga tttgcgctcg ctcgaccggc aaaagaagct gctcgacgat acggtcaccg cctacgccgc tgcactgcgg atcgtgcaac agcaactgaa ggacggcgcg atcgatgcgt ctgccgtcgc gcaggcgcag acgcagctgg aaagcacgcg cacgcaagat agcgacatcg atgtgcagcg cgcgcagctc gagcatgcga tcgccacgct ggtcggtcag cccgcatcgt ctttctcgct accgccgaag gtggagaccg tcgcgctgcc gcagattccc gcgggcgtgc cgtcgcaatt gctggagcgc cgtcccgaca tcgccgccgc cgaacggcgc gtcgccgccg caaacgcgca gatcggcgaa gcgcgcgcgg cgttctatcc gaacctgacg ctgtcggcga cggcggggct cgaaagctcg ttcttcgcgc cgtggctcac ggcatcgagc ctgttctggt cgctcggctc acagatcgcg ggtacgctgt tcgacggcgg ccggcgcgcg gcagcgctga acaatgcaag cgcgcaatac gacggcacgg tcgccgatta ccggcaaacg gtgctggtgg ccttccagca ggtcgaggac gatctgtcgt cgctcaatac gctcgcgaac gaagccgaca gccagcaacg cgcgacagcg gccgccgacc tgtcgctcaa gctgacgacc aatcgctatc aggcgggtgc cgtcgactat ctgaacgtgg tgacagcaca aaccatcgcg ctgtccaacg agcgcgcggc ggaacagatc gacgcgcgcc gtatcgatgc aagcgtgcaa ttgctgaaag cgctcggcgg cggctgggac cgcaatgcgt tgacggatgc aaacgcaacg gattaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa