BuphA.01673.p

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuphA.01673.p
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01673.p.A2.GE32188 Mature protein( BuphA.01673.p ) 28 484
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4752
RefSeq: YP_001860655.1
UniProt: B2JQR8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKSNVARALA VAAAMTLAAC AVQPGTHADL PQTVKTVAPD AWSVDAPNDT VDADQWWSQF GDPTMHKLVD LVLNDNLDVK AAVERVKQAG EVTKQQRAAL SPQLDAGATA AYERQNTPPP LGYVKQAGAG LTASWQPDVF GGERLAVLAA QAQATGRQWA LNELRLALAA NTAAAYIDLR WAQTQRQIIN ENEDIRSRAL KLTQQRLKYG LSTQLDVARA QNQLQDLQAQ IPKINSTIQH DMSLIAVYSG RTPESVDSLL LSDAQPIPVP SQSVPLTLPS EALLRRPDVR TAYATVEQRA AEVGVARADR YPKFSLRLSD GILASSYLGM PTLTDNLFSA ALNATAPIFN AGRITAHIEE NESRMRESQL NLQQTMLNAL KDIEDTRSDL VNGDAQVAKL AGALDASGHA LRLSTELYKG GASSFLDVLD AQEAYLRDSE SLNQAKREHA QAAVALYRSL GGGWDAPAAS DAAKARPTTD VAAN
NT Sequence
ATGAAATCGA ACGTAGCCAG AGCGCTCGCC GTTGCCGCCG CCATGACGCT TGCGGCCTGT GCCGTGCAGC CCGGGACGCA CGCGGACTTG CCGCAGACGG TGAAGACCGT CGCGCCCGAC GCGTGGAGCG TCGACGCGCC GAACGACACG GTGGACGCCG ACCAGTGGTG GTCGCAGTTC GGCGACCCGA CGATGCACAA ACTCGTCGAC CTCGTGCTCA ACGACAACCT CGACGTCAAG GCGGCCGTCG AACGCGTGAA GCAGGCCGGG GAGGTGACGA AGCAGCAGCG CGCCGCGTTG TCGCCGCAAC TCGATGCAGG CGCGACGGCT GCGTATGAGC GGCAAAATAC GCCGCCGCCG CTCGGCTATG TGAAGCAGGC GGGGGCAGGT CTGACGGCCT CGTGGCAGCC CGATGTGTTC GGCGGCGAAC GTCTCGCCGT GCTCGCCGCG CAGGCACAGG CGACGGGCCG TCAATGGGCG CTGAACGAAT TGCGGCTCGC GCTCGCCGCG AATACAGCCG CTGCATATAT CGATTTGCGC TGGGCGCAGA CGCAACGGCA GATCATCAAC GAGAACGAGG ACATTCGCAG CCGCGCGCTG AAGCTTACGC AGCAGCGCCT GAAATACGGG CTCTCGACGC AGCTCGATGT CGCGCGTGCG CAAAACCAGC TGCAGGACTT GCAGGCGCAG ATTCCGAAGA TCAACTCGAC CATCCAGCAC GACATGAGCC TGATCGCAGT GTACTCGGGA CGTACGCCGG AAAGCGTCGA CAGCCTGCTG CTCAGCGACG CGCAGCCGAT TCCCGTACCG TCGCAAAGCG TGCCCCTGAC GCTGCCTTCC GAAGCGCTGC TGCGCCGCCC CGATGTGCGC ACCGCCTACG CGACTGTCGA ACAGCGCGCC GCCGAAGTGG GCGTCGCGCG AGCGGACCGC TATCCGAAGT TCAGCCTGCG CCTGAGCGAC GGCATTCTCG CGTCGTCGTA TCTCGGCATG CCGACATTGA CGGACAACCT GTTCAGCGCA GCATTGAACG CTACCGCGCC GATCTTCAAT GCAGGCCGCA TCACCGCGCA TATCGAGGAG AACGAGAGCC GCATGCGCGA ATCGCAATTG AACCTGCAGC AGACGATGCT GAATGCGCTG AAGGATATCG AGGACACGCG CAGCGATCTC GTCAACGGCG ACGCACAGGT CGCGAAGCTT GCTGGTGCGC TCGATGCATC GGGGCATGCG TTGCGTCTGT CGACGGAGCT GTACAAGGGC GGCGCGTCGA GCTTCCTCGA TGTGCTCGAT GCGCAGGAAG CGTATCTGCG CGATTCGGAA TCGTTGAATC AGGCGAAGCG TGAGCATGCG CAAGCTGCTG TTGCGTTGTA TCGCTCGCTC GGCGGCGGCT GGGATGCGCC CGCCGCCAGT GACGCAGCGA AGGCCAGGCC GACGACCGAT GTCGCCGCGA AC
Details for BuphA.01673.p.A2.GE32188
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 51kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MADLPQTVKT VAPDAWSVDA PNDTVDADQW WSQFGDPTMH KLVDLVLNDN LDVKAAVERV KQAGEVTKQQ RAALSPQLDA GATAAYERQN TPPPLGYVKQ AGAGLTASWQ PDVFGGERLA VLAAQAQATG RQWALNELRL ALAANTAAAY IDLRWAQTQR QIINENEDIR SRALKLTQQR LKYGLSTQLD VARAQNQLQD LQAQIPKINS TIQHDMSLIA VYSGRTPESV DXXLLSDAQP IPVPSQSVPL TLPSEALLRR PDVRTAYATV EQRAAEVGVA RADRYPKFSL RLSDGILASS YLGMPTLTDN LFSAALNATA PIFNAGRITA HIEENESRMR ESQLNLQQTM LNALKDIEDT RSDLVNGDAQ VAKLAGALDA SGHALRLSTE LYKGGASSFL DVLDAQEAYL RDSESLNQAK REHAQAAVAL YRSLGGGWDA PAASDAAKAR PTTDVAAN
Validated NT Sequence
ttattagttc gcggcgacat cggtcgtcgg cctggccttc gctgcgtcac tggcggcggg cgcatcccag ccgccgccga gcgagcgata caacgcaaca gcagcttgcg catgctcacg cttcgcctga ttcaacgatt ccgaatcgcg cagatacgct tcctgcgcat cgagcacatc gaggaagctc gacgcgccgc ccttgtacag ctccgtcgac agacgcaacg catgccccga tgcatcgagc gcaccagcaa gcttcgcgac ctgtgcgtcg ccgttgacga gatcgctgcg cgtgtcctcg atatccttca gcgcattcag catcgtctgc tgcaggttca attgcgattc gcgcatgcgg ctctcgttct cctcgatatg cgcggtgatg cggcctgcat tgaagatcgg cgcggtagcg ttcaatgctg cgctgaacag gttgtccgtc aatgtcggca tgccgagata cgacgacgcg agaatgccgt cgctcaggcg caggctgaac ttcggatagc ggtccgctcg cgcgacgccc acttcggcgg cgcgctgttc gacagtcgcg taggcggtgc gcacatcggg gcggcgcagc agcgcttcgg aaggcagcgt caggggcacg ctttgcgacg gtacgggaat cggctgcgcg tcgctgagca gcannctgtc gacgctttcc ggcgtacgtc ccgagtacac tgcgatcagg ctcatgtcgt gctggatggt cgagttgatc ttcggaatct gcgcctgcaa gtcctgcagc tggttttgcg cacgcgcgac atcgagctgc gtcgagagcc cgtatttcag gcgctgctgc gtaagcttca gcgcgcggct gcgaatgtcc tcgttctcgt tgatgatctg ccgttgcgtc tgcgcccagc gcaaatcgat atatgcagcg gctgtattcg cggcgagcgc gagccgcaat tcgttcagcg cccattgacg gcccgtcgcc tgtgcctgcg cggcgagcac ggcgagacgt tcgccgccga acacatcggg ctgccacgag gccgtcagac ctgcccccgc ctgcttcaca tagccgagcg gcggcggcgt attttgccgc tcatacgcag ccgtcgcgcc tgcatcgagt tgcggcgaca acgcggcgcg ctgctgcttc gtcacctccc cggcctgctt cacgcgttcg acggccgcct tgacgtcgag gttgtcgttg agcacgaggt cgacgagttt gtgcatcgtc gggtcgccga actgcgacca ccactggtcg gcgtccaccg tgtcgttcgg cgcgtcgacg ctccacgcgt cgggcgcgac ggtcttcacc gtctgcggca agtccgccat cgaaccagga cc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMADLPQTVK TVAPDAWSVD APNDTVDADQ WWSQFGDPTM HKLVDLVLND NLDVKAAVER VKQAGEVTKQ QRAALSPQLD AGATAAYERQ NTPPPLGYVK QAGAGLTASW QPDVFGGERL AVLAAQAQAT GRQWALNELR LALAANTAAA YIDLRWAQTQ RQIINENEDI RSRALKLTQQ RLKYGLSTQL DVARAQNQLQ DLQAQIPKIN STIQHDMSLI AVYSGRTPES VDSLLLSDAQ PIPVPSQSVP LTLPSEALLR RPDVRTAYAT VEQRAAEVGV ARADRYPKFS LRLSDGILAS SYLGMPTLTD NLFSAALNAT APIFNAGRIT AHIEENESRM RESQLNLQQT MLNALKDIED TRSDLVNGDA QVAKLAGALD ASGHALRLST ELYKGGASSF LDVLDAQEAY LRDSESLNQA KREHAQAAVA LYRSLGGGWD APAASDAAKA RPTTDVAAN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggacttg ccgcagacgg tgaagaccgt cgcgcccgac gcgtggagcg tcgacgcgcc gaacgacacg gtggacgccg accagtggtg gtcgcagttc ggcgacccga cgatgcacaa actcgtcgac ctcgtgctca acgacaacct cgacgtcaag gcggccgtcg aacgcgtgaa gcaggccggg gaggtgacga agcagcagcg cgccgcgttg tcgccgcaac tcgatgcagg cgcgacggct gcgtatgagc ggcaaaatac gccgccgccg ctcggctatg tgaagcaggc gggggcaggt ctgacggcct cgtggcagcc cgatgtgttc ggcggcgaac gtctcgccgt gctcgccgcg caggcacagg cgacgggccg tcaatgggcg ctgaacgaat tgcggctcgc gctcgccgcg aatacagccg ctgcatatat cgatttgcgc tgggcgcaga cgcaacggca gatcatcaac gagaacgagg acattcgcag ccgcgcgctg aagcttacgc agcagcgcct gaaatacggg ctctcgacgc agctcgatgt cgcgcgtgcg caaaaccagc tgcaggactt gcaggcgcag attccgaaga tcaactcgac catccagcac gacatgagcc tgatcgcagt gtactcggga cgtacgccgg aaagcgtcga cagcctgctg ctcagcgacg cgcagccgat tcccgtaccg tcgcaaagcg tgcccctgac gctgccttcc gaagcgctgc tgcgccgccc cgatgtgcgc accgcctacg cgactgtcga acagcgcgcc gccgaagtgg gcgtcgcgcg agcggaccgc tatccgaagt tcagcctgcg cctgagcgac ggcattctcg cgtcgtcgta tctcggcatg ccgacattga cggacaacct gttcagcgca gcattgaacg ctaccgcgcc gatcttcaat gcaggccgca tcaccgcgca tatcgaggag aacgagagcc gcatgcgcga atcgcaattg aacctgcagc agacgatgct gaatgcgctg aaggatatcg aggacacgcg cagcgatctc gtcaacggcg acgcacaggt cgcgaagctt gctggtgcgc tcgatgcatc ggggcatgcg ttgcgtctgt cgacggagct gtacaagggc ggcgcgtcga gcttcctcga tgtgctcgat gcgcaggaag cgtatctgcg cgattcggaa tcgttgaatc aggcgaagcg tgagcatgcg caagctgctg ttgcgttgta tcgctcgctc ggcggcggct gggatgcgcc cgccgccagt gacgcagcga aggccaggcc gacgaccgat gtcgccgcga actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa