SSGCID: Target Page

BuphA.01673.o

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID:	BuphA.01673.o
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.01673.o.A2.GE32189	Mature protein( BuphA.01673.o )		24	484

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.01673.o.A2.PS01144	Mature protein( BuphA.01673.o )		24	484

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.4570
RefSeq:	YP_001860488.1
UniProt:	B2JQA1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKRYVPLFAL ALCGCAVGPD FHAPAPPDAP SYMRETLPAT TQAAGAAQTL ASSDHALPTW WTQFQSDALN RLVDTALQHS PTLDEARAKL VQARENYVAQ AGATQFPAID ATLSGSRQKV DPAAFGIPNV QNPGPFTLFN ASVSVSYALD VFGGNRRALE ALLAQVDYQT YELQAARLTL AGNVVSAVIR RASLQQQLAL TRQLANAQAR QLTIVEGRYA AGGVAQLDVR TQHTLLAQTR AQIPPLETQL AQADHQLAIL LGIVPSQANF DDITLDALHL PDAVPVTLPS TLARERPDIR ASEALLHQAS ADVGVATANL YPQFIVSAGF GSERTRIEDV VNGLNVWNIG LNLAQPIFHG GELRAKKRAA QAAYDAAFAS YRQTVLKALQ QVADTLHALN DDAHALQARD DANAEAQASA KIAQAQYAAG GVSQFGLIDT QRQALQTALD RTRAQADRLV DTAALFQALG GMTASEQADQ TAGR

NT Sequence

ATGAAACGCT ACGTGCCCTT GTTCGCGCTC GCGCTGTGCG GCTGCGCGGT CGGCCCGGAC TTTCACGCGC CTGCTCCGCC CGATGCGCCG TCGTACATGC GCGAAACACT GCCCGCTACG ACGCAGGCCG CGGGCGCCGC GCAAACCCTC GCTTCCTCCG ACCATGCGCT GCCCACATGG TGGACGCAGT TTCAATCCGA CGCGCTGAAC CGTCTTGTCG ATACAGCACT GCAACATAGT CCGACGCTCG ATGAAGCGCG CGCAAAACTC GTGCAAGCGC GCGAAAACTA CGTTGCGCAA GCGGGCGCGA CGCAGTTCCC CGCAATCGAT GCGACGCTCT CGGGCTCGCG TCAGAAGGTC GATCCCGCCG CGTTCGGCAT TCCGAATGTG CAGAACCCTG GGCCGTTCAC ATTGTTCAAC GCATCGGTAA GCGTGTCGTA TGCGCTCGAC GTGTTCGGCG GCAACCGGCG CGCGCTCGAA GCACTGCTCG CGCAAGTCGA CTATCAAACG TATGAACTGC AGGCTGCCCG CCTGACGCTC GCGGGCAACG TCGTATCGGC GGTGATCCGG CGCGCGTCGC TGCAACAGCA GCTTGCGCTG ACGCGGCAAC TCGCGAATGC GCAGGCTCGG CAGTTGACGA TTGTCGAAGG ACGCTATGCG GCGGGTGGCG TTGCGCAACT GGATGTACGC ACGCAGCACA CGCTGCTCGC GCAGACCCGC GCGCAGATTC CGCCGCTCGA GACACAACTC GCGCAGGCCG ACCATCAGCT CGCGATTCTG CTTGGCATCG TTCCGTCGCA AGCGAATTTC GACGACATCA CGCTCGATGC GCTGCATCTG CCCGACGCCG TGCCCGTCAC GCTGCCATCG ACGCTCGCGC GCGAAAGGCC CGACATCCGC GCGTCGGAGG CGTTGTTGCA TCAGGCGAGC GCGGACGTCG GCGTCGCGAC GGCGAACCTG TATCCGCAGT TCATCGTGTC GGCGGGATTC GGTTCGGAGC GCACGCGCAT CGAGGATGTC GTCAATGGAT TGAACGTCTG GAATATCGGC CTGAATCTGG CGCAACCGAT TTTTCACGGC GGTGAACTGC GCGCGAAGAA ACGCGCCGCG CAGGCGGCTT ACGATGCGGC TTTCGCGTCG TATCGGCAAA CGGTGCTGAA AGCGCTGCAG CAAGTCGCCG ATACGCTGCA CGCACTGAAC GACGATGCGC ACGCATTGCA GGCGCGCGAT GACGCGAACG CCGAAGCGCA GGCAAGCGCA AAAATCGCGC AGGCGCAATA TGCGGCGGGC GGCGTTAGTC AGTTCGGCTT GATCGATACG CAGCGTCAGG CATTGCAGAC GGCGCTCGAC AGGACGCGCG CGCAGGCCGA TCGTCTCGTG GACACGGCGG CGCTGTTTCA GGCGTTAGGC GGGATGACGG CAAGCGAACA GGCGGATCAG ACAGCAGGAC GT

Details for BuphA.01673.o.A2.GE32189

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	51kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	57
PERCENT COVERAGE:	60

Validated AA Sequence

MPAPPDAPSY MRETLPATTQ AAGAAQTLAS SDHALPTWWT QFQSDALNRL XXTALXHSPT LDEARAKLVQ ARENYVAQAG ATQFPAIXAT LSGSRXKVDP AAFGIPNVQN PGPFTLFNAS XXVSYALDVF XGNRXALEXL XXQXDYQTYE LXAARLTLXX XVXXXXXXRX SXQQXXALTR QLXXXXXSAV DXCRRXLCGG VXXXXLDVXX HXRXXRXXXX XXPPXXDXTX AXXXSXRXXX XXXSXXXXFR XVXXXXXXLX XXXXXXXXXX WSXXXXPXXX XXXXXXXIXA XXX

Validated NT Sequence

gcnnnncagc catgtttacn nncaccnnnn nnnnannnnn annnnnnnan aannnnnnnn nannnnnnnn nnnannnngg tcctggttcn atgcctgctc cgcccgatgc gccgtcgtac atgcgcgaaa cactgcccgc tacgacgcag gccgcgggcg ccgcgcaaac cctcgcttcc tccgaccatg cgctgcccac atggtggacg cagtttcaat ccgacgcgct gaaccgtctt gnnnatacag cnctgcnaca tagtccgacg ctcgatgaag cgcgcgcaaa actcgtgcaa gcgcgcgaaa actacgttgc gcaagcgggc gcgacgcagt tccccgcaat cnatgcgacg ctctcgggct cgcgtcanaa ggtcgatccc gccgcgttcg gcattccgaa tgtgcagaac cctgggccgt tcacattgtt caacgcatcg nnaancgtgt cgtatgcgct cgacgtgttc ngcggcaacc ggngcgcgct cgaancactg ntcgngcaan tcgactatca aacgtatgaa ctgnnggctg cccgcctgac gctcgnnnnn nacgtcntat ngnnngngat nnggcgcgng tcgntgcaac agcnnnttgc gctgacncgg caactcncgn anngcncang ntcngcagtt gacnattgtc gaaganggct ntgcggcggg gtggnngntn cncanctgga tgtacnnncg cacanacgnt gnnngcgcan acnngngcnn nnanttccgc cnnntcnaga canaactcan gcannnngan natcnnctcg ngnttnnnct tgnnnncnnt ccntcgnann cnnatttcga ngagtcannn nnnnngnnnn ncntctgcnn nannccnnnn nnnnnnnnnc nnnnannnac tggtcgnncg nnaannnccc aanntnnnnn ncnnnnnann gnnnnnatng natccnggcn ancncnnann t

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMPAPPDAPS YMRETLPATT QAAGAAQTLA SSDHALPTWW TQFQSDALNR LVDTALQHSP TLDEARAKLV QARENYVAQA GATQFPAIDA TLSGSRQKVD PAAFGIPNVQ NPGPFTLFNA SVSVSYALDV FGGNRRALEA LLAQVDYQTY ELQAARLTLA GNVVSAVIRR ASLQQQLALT RQLANAQARQ LTIVEGRYAA GGVAQLDVRT QHTLLAQTRA QIPPLETQLA QADHQLAILL GIVPSQANFD DITLDALHLP DAVPVTLPST LARERPDIRA SEALLHQASA DVGVATANLY PQFIVSAGFG SERTRIEDVV NGLNVWNIGL NLAQPIFHGG ELRAKKRAAQ AAYDAAFASY RQTVLKALQQ VADTLHALND DAHALQARDD ANAEAQASAK IAQAQYAAGG VSQFGLIDTQ RQALQTALDR TRAQADRLVD TAALFQALGG MTASEQADQT AGR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcctgctccg cccgatgcgc cgtcgtacat gcgcgaaaca ctgcccgcta cgacgcaggc cgcgggcgcc gcgcaaaccc tcgcttcctc cgaccatgcg ctgcccacat ggtggacgca gtttcaatcc gacgcgctga accgtcttgt cgatacagca ctgcaacata gtccgacgct cgatgaagcg cgcgcaaaac tcgtgcaagc gcgcgaaaac tacgttgcgc aagcgggcgc gacgcagttc cccgcaatcg atgcgacgct ctcgggctcg cgtcagaagg tcgatcccgc cgcgttcggc attccgaatg tgcagaaccc tgggccgttc acattgttca acgcatcggt aagcgtgtcg tatgcgctcg acgtgttcgg cggcaaccgg cgcgcgctcg aagcactgct cgcgcaagtc gactatcaaa cgtatgaact gcaggctgcc cgcctgacgc tcgcgggcaa cgtcgtatcg gcggtgatcc ggcgcgcgtc gctgcaacag cagcttgcgc tgacgcggca actcgcgaat gcgcaggctc ggcagttgac gattgtcgaa ggacgctatg cggcgggtgg cgttgcgcaa ctggatgtac gcacgcagca cacgctgctc gcgcagaccc gcgcgcagat tccgccgctc gagacacaac tcgcgcaggc cgaccatcag ctcgcgattc tgcttggcat cgttccgtcg caagcgaatt tcgacgacat cacgctcgat gcgctgcatc tgcccgacgc cgtgcccgtc acgctgccat cgacgctcgc gcgcgaaagg cccgacatcc gcgcgtcgga ggcgttgttg catcaggcga gcgcggacgt cggcgtcgcg acggcgaacc tgtatccgca gttcatcgtg tcggcgggat tcggttcgga gcgcacgcgc atcgaggatg tcgtcaatgg attgaacgtc tggaatatcg gcctgaatct ggcgcaaccg atttttcacg gcggtgaact gcgcgcgaag aaacgcgccg cgcaggcggc ttacgatgcg gctttcgcgt cgtatcggca aacggtgctg aaagcgctgc agcaagtcgc cgatacgctg cacgcactga acgacgatgc gcacgcattg caggcgcgcg atgacgcgaa cgccgaagcg caggcaagcg caaaaatcgc gcaggcgcaa tatgcggcgg gcggcgttag tcagttcggc ttgatcgata cgcagcgtca ggcattgcag acggcgctcg acaggacgcg cgcgcaggcc gatcgtctcg tggacacggc ggcgctgttt caggcgttag gcgggatgac ggcaagcgaa caggcggatc agacagcagg acgttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BuphA.01673.o.A2.PS01144

PURIFICATION DATe:	7/6/2011
CONCENTRATION:	27.8mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	17
VIAL VOLUME:	200µl
PERCENT IDENTITY:	57
PERCENT COVERAGE:	60

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCTGCTCCG CCCGATGCGC CGTCGTACAT GCGCGAAACA CTGCCCGCTA CGACGCAGGC CGCGGGCGCC GCGCAAACCC TCGCTTCCTC CGACCATGCG CTGCCCACAT GGTGGACGCA GTTTCAATCC GACGCGCTGA ACCGTCTTGT CGATACAGCA CTGCAACATA GTCCGACGCT CGATGAAGCG CGCGCAAAAC TCGTGCAAGC GCGCGAAAAC TACGTTGCGC AAGCGGGCGC GACGCAGTTC CCCGCAATCG ATGCGACGCT CTCGGGCTCG CGTCAGAAGG TCGATCCCGC CGCGTTCGGC ATTCCGAATG TGCAGAACCC TGGGCCGTTC ACATTGTTCA ACGCATCGGT AAGCGTGTCG TATGCGCTCG ACGTGTTCGG CGGCAACCGG CGCGCGCTCG AAGCACTGCT CGCGCAAGTC GACTATCAAA CGTATGAACT GCAGGCTGCC CGCCTGACGC TCGCGGGCAA CGTCGTATCG GCGGTGATCC GGCGCGCGTC GCTGCAACAG CAGCTTGCGC TGACGCGGCA ACTCGCGAAT GCGCAGGCTC GGCAGTTGAC GATTGTCGAA GGACGCTATG CGGCGGGTGG CGTTGCGCAA CTGGATGTAC GCACGCAGCA CACGCTGCTC GCGCAGACCC GCGCGCAGAT TCCGCCGCTC GAGACACAAC TCGCGCAGGC CGACCATCAG CTCGCGATTC TGCTTGGCAT CGTTCCGTCG CAAGCGAATT TCGACGACAT CACGCTCGAT GCGCTGCATC TGCCCGACGC CGTGCCCGTC ACGCTGCCAT CGACGCTCGC GCGCGAAAGG CCCGACATCC GCGCGTCGGA GGCGTTGTTG CATCAGGCGA GCGCGGACGT CGGCGTCGCG ACGGCGAACC TGTATCCGCA GTTCATCGTG TCGGCGGGAT TCGGTTCGGA GCGCACGCGC ATCGAGGATG TCGTCAATGG ATTGAACGTC TGGAATATCG GCCTGAATCT GGCGCAACCG ATTTTTCACG GCGGTGAACT GCGCGCGAAG AAACGCGCCG CGCAGGCGGC TTACGATGCG GCTTTCGCGT CGTATCGGCA AACGGTGCTG AAAGCGCTGC AGCAAGTCGC CGATACGCTG CACGCACTGA ACGACGATGC GCACGCATTG CAGGCGCGCG ATGACGCGAA CGCCGAAGCG CAGGCAAGCG CAAAAATCGC GCAGGCGCAA TATGCGGCGG GCGGCGTTAG TCAGTTCGGC TTGATCGATA CGCAGCGTCA GGCATTGCAG ACGGCGCTCG ACAGGACGCG CGCGCAGGCC GATCGTCTCG TGGACACGGC GGCGCTGTTT CAGGCGTTAG GCGGGATGAC GGCAAGCGAA CAGGCGGATC AGACAGCAGG ACGTTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA