SSGCID: Target Page

BuphA.01673.n

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID:	BuphA.01673.n
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.01673.n.A2.GE34740	Mature protein( BuphA.01673.n )		34	502

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.01673.n.A2.PW35350	Mature protein( BuphA.01673.n )		34	502

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.4245
RefSeq:	YP_001860191.1
UniProt:	B2JPE2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKNQSNAGRR ASGALKMGVS VMFAAVLSAC VNYAGIHSDA KTTEPQQYAT QQSIPAESGH WPAANWADQF GDAQLKALID EALKGSPTLE QARARVASAS AYSETAKAST MPRVDADYSL TRQQYSGTAL VPPPTGGSWQ TENKGLLSAS YDLDLWGKNR EALKAAISQL QASQADAEVV KLTLTTSIAR TYNQLARLYA LRDIAQQEIV QREQIDRITA GRIATGLDTE VERKTAQANL ATSRATLKSL DGQILATRYQ IAALLGAGPD RGLQIARPTL GIGDDVNLPD NLPADLVSRR PDIVAARWRV DAMTHDVKEA KAEFYPDINL SAAIGLDAFG FGRFLTAASR TASVGPAIHL PIFDAGELRA QLKGRYADFD YAVATYNQTL VTALSEVATQ LAGVRSTDGQ LVDAQTAQDA ARQADSLALV QYKAGLTNQL TVLNADVNAL AADQAVANLR MDRRDQQIAL ASALGGGFVD TSNTDQNAHV TSAPETSANA AH

NT Sequence

ATGAAAAATC AATCAAATGC CGGACGGCGC GCTTCCGGCG CGCTGAAAAT GGGCGTGTCC GTGATGTTCG CTGCCGTGCT GTCGGCGTGC GTCAACTACG CGGGCATTCA TAGCGATGCG AAGACGACCG AACCGCAGCA GTACGCGACG CAGCAAAGCA TTCCCGCCGA GTCGGGCCAT TGGCCTGCCG CCAATTGGGC CGATCAGTTC GGCGACGCGC AACTGAAGGC GCTGATCGAC GAGGCACTGA AGGGCAGTCC GACGCTTGAA CAGGCCCGCG CCCGTGTGGC ATCGGCGTCG GCGTATAGCG AAACGGCGAA GGCAAGCACG ATGCCGCGCG TCGACGCCGA CTATTCGCTT ACGCGTCAGC AGTATTCGGG CACCGCGCTC GTGCCGCCGC CGACGGGTGG CTCGTGGCAG ACGGAGAACA AGGGTCTGCT GAGCGCTTCG TACGATCTCG ACCTGTGGGG CAAGAACCGC GAAGCGCTGA AGGCGGCGAT CTCGCAACTG CAGGCGAGCC AGGCCGATGC CGAAGTCGTC AAGCTCACGC TGACGACTTC GATTGCGCGC ACGTACAACC AGCTTGCGCG TCTGTATGCA TTGCGCGATA TCGCGCAGCA GGAGATCGTA CAGCGCGAAC AGATCGACAG AATTACGGCG GGCCGCATCG CGACGGGCCT CGATACGGAA GTCGAGCGCA AGACGGCTCA GGCGAATCTC GCGACGAGCC GCGCCACGTT GAAGTCGCTC GACGGTCAGA TTCTTGCCAC GCGATATCAG ATCGCCGCGC TGCTCGGCGC TGGCCCGGAC CGTGGACTGC AGATCGCGCG TCCCACGCTC GGCATCGGCG ACGACGTGAA TCTGCCCGAC AACCTCCCCG CGGATCTGGT AAGCCGCCGT CCCGACATCG TCGCCGCACG CTGGCGCGTC GATGCAATGA CGCACGACGT AAAGGAGGCG AAAGCCGAGT TCTATCCCGA CATCAACCTG AGCGCGGCGA TCGGACTCGA CGCGTTCGGT TTCGGCCGAT TCTTGACGGC GGCGAGCCGC ACGGCATCCG TCGGTCCCGC GATACATCTG CCCATCTTCG ATGCCGGCGA ACTACGCGCG CAACTGAAGG GCCGTTACGC CGATTTCGAC TATGCCGTCG CAACCTACAA CCAGACGCTC GTTACCGCGT TGAGCGAGGT TGCCACGCAA CTTGCCGGCG TGCGTTCGAC GGACGGCCAA CTCGTCGACG CGCAAACCGC ACAGGACGCC GCGCGCCAGG CCGATTCGCT CGCACTCGTG CAATACAAGG CCGGGCTCAC GAACCAGCTG ACGGTATTGA ATGCCGATGT GAATGCGCTT GCCGCCGATC AGGCCGTTGC GAACCTGCGA ATGGACCGCC GCGATCAGCA GATCGCGCTG GCATCCGCGC TGGGCGGCGG CTTCGTCGAT ACCTCGAACA CGGACCAGAA CGCGCACGTC ACGTCCGCCC CTGAAACGTC TGCCAACGCC GCGCAT

Details for BuphA.01673.n.A2.GE34740

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/12/2012
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMAGIHSDAK TTEPQQYATQ QSIPAESGHW PAANWADQFG DAQLKALIDE ALKGSPTLEQ ARARVASASA YSETAKASTM PRVDADYSLT RQQYSGTALV PPPTGGSWQT ENKGLLSASY DLDLWGKNRE ALKAAISQLQ ASQADAEXVK LTLTTSIART YXXLARLYAL RDIAQXEIVQ REQIDRITAG RIATGLDTEV ERKTAQANLA TSRATLKSLD XQILATRYQI AALLGAGPDR GLQIARPTLG IGDDVNLPDN XPADLVSRRP DIVAARWRVD AMTHDVKEAK AEFYPDINLS AAIGLDAFGF GRFLTAASRT ASVGPAIHLP IFDAGELRAQ LKGRYADFDY AVATYNQTLV TALSEVATQL AGVRSTDGQL VDAQTAQDAA RQADSLALVQ YKAGLTNQLT VLNADVNALA ADQAVANLRM DRRDQQIALA SALGGGFVDT SNTDQNAHVT SAPETSANAA

Validated NT Sequence

atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg gcattcatag cgatgcgaag acgaccgaac cgcagcagta cgcgacgcag caaagcattc ccgccgagtc gggccattgg cctgccgcca attgggccga tcagttcggc gacgcgcaac tgaaggcgct gatcgacgag gcactgaagg gcagtccgac gcttgaacag gcccgcgccc gtgtggcatc ggcgtcggcg tatagcgaaa cggcgaaggc aagcacgatg ccgcgcgtcg acgccgacta ttcgcttacg cgtcagcagt attcgggcac cgcgctcgtg ccgccgccga cgggtggctc gtggcagacg gagaacaagg gtctgctgag cgcttcgtac gatctcgacc tgtggggcaa gaaccgcgaa gcgctgaagg cggcgatctc gcaactgcag gcgagccagg ccgatgccga antcgtcaag ctcacgctga cgacttcnat tgcgcgcacg tacnaccngc ttgcgcgtct gtatgcattg cgcgatatcg cgcagcanga gatcgtacag cgcgaacaga tcgacagaat tacngcgggc cgcatcgcga cgggcctcga tacggaagtc gagcgcaaga cggctcaggc gaatctcgcg acgagccgcg ccacgttgaa gtcgctcgac gntcagattc ttgccacgcg atatcagatc gccgcgctgc tcggcgctgg cccggaccgt ggactgcaga tcgcgcgtcc cacgctcggc atcggcgacg acgtgaatct gcccgacaac ntccccgcgg atctggtaag ccgccgtccc gacatcgtcg ccgcacgctg gcgcgtcgat gcaatgacgc acgacgtaaa ggaggcgaaa gccgagttct atcccgacat caacctgagc gcggcgatcg gactcgacgc gttcggtttc ggccgattct tgacggcggc gagccgcacg gcatccgtcg gtcccgcgat acatctgccc atcttcgatg ccggcgaact acgcgcgcaa ctgaagggcc gttacgccga tttcgactat gccgtcgcaa cctacaacca gacgctcgtt accgcgttga gcgaggttgc cacgcaactt gccggcgtgc gttcgacgga cggccaactc gtcgacgcgc aaaccgcaca ggacgccgcg cgccaggccg attcgctcgc actcgtgcaa tacaaggccg ggctcacgaa ccagctgacg gtattgaatg ccgatgtgaa tgcgcttgcc gccgatcagg ccgttgcgaa cctgcgaatg gaccgccgcg atcagcagat cgcgctggca tccgcgctgg gcggcggctt cgtcgatacc tcgaacacgg accagaacgc gcacgtcacg tccgcccctg aaacgtctgc caacgccgcg cat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAGIHSDAK TTEPQQYATQ QSIPAESGHW PAANWADQFG DAQLKALIDE ALKGSPTLEQ ARARVASASA YSETAKASTM PRVDADYSLT RQQYSGTALV PPPTGGSWQT ENKGLLSASY DLDLWGKNRE ALKAAISQLQ ASQADAEVVK LTLTTSIART YNQLARLYAL RDIAQQEIVQ REQIDRITAG RIATGLDTEV ERKTAQANLA TSRATLKSLD GQILATRYQI AALLGAGPDR GLQIARPTLG IGDDVNLPDN LPADLVSRRP DIVAARWRVD AMTHDVKEAK AEFYPDINLS AAIGLDAFGF GRFLTAASRT ASVGPAIHLP IFDAGELRAQ LKGRYADFDY AVATYNQTLV TALSEVATQL AGVRSTDGQL VDAQTAQDAA RQADSLALVQ YKAGLTNQLT VLNADVNALA ADQAVANLRM DRRDQQIALA SALGGGFVDT SNTDQNAHVT SAPETSANAA H

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgggcatt catagcgatg cgaagacgac cgaaccgcag cagtacgcga cgcagcaaag cattcccgcc gagtcgggcc attggcctgc cgccaattgg gccgatcagt tcggcgacgc gcaactgaag gcgctgatcg acgaggcact gaagggcagt ccgacgcttg aacaggcccg cgcccgtgtg gcatcggcgt cggcgtatag cgaaacggcg aaggcaagca cgatgccgcg cgtcgacgcc gactattcgc ttacgcgtca gcagtattcg ggcaccgcgc tcgtgccgcc gccgacgggt ggctcgtggc agacggagaa caagggtctg ctgagcgctt cgtacgatct cgacctgtgg ggcaagaacc gcgaagcgct gaaggcggcg atctcgcaac tgcaggcgag ccaggccgat gccgaagtcg tcaagctcac gctgacgact tcgattgcgc gcacgtacaa ccagcttgcg cgtctgtatg cattgcgcga tatcgcgcag caggagatcg tacagcgcga acagatcgac agaattacgg cgggccgcat cgcgacgggc ctcgatacgg aagtcgagcg caagacggct caggcgaatc tcgcgacgag ccgcgccacg ttgaagtcgc tcgacggtca gattcttgcc acgcgatatc agatcgccgc gctgctcggc gctggcccgg accgtggact gcagatcgcg cgtcccacgc tcggcatcgg cgacgacgtg aatctgcccg acaacctccc cgcggatctg gtaagccgcc gtcccgacat cgtcgccgca cgctggcgcg tcgatgcaat gacgcacgac gtaaaggagg cgaaagccga gttctatccc gacatcaacc tgagcgcggc gatcggactc gacgcgttcg gtttcggccg attcttgacg gcggcgagcc gcacggcatc cgtcggtccc gcgatacatc tgcccatctt cgatgccggc gaactacgcg cgcaactgaa gggccgttac gccgatttcg actatgccgt cgcaacctac aaccagacgc tcgttaccgc gttgagcgag gttgccacgc aacttgccgg cgtgcgttcg acggacggcc aactcgtcga cgcgcaaacc gcacaggacg ccgcgcgcca ggccgattcg ctcgcactcg tgcaatacaa ggccgggctc acgaaccagc tgacggtatt gaatgccgat gtgaatgcgc ttgccgccga tcaggccgtt gcgaacctgc gaatggaccg ccgcgatcag cagatcgcgc tggcatccgc gctgggcggc ggcttcgtcg atacctcgaa cacggaccag aacgcgcacg tcacgtccgc ccctgaaacg tctgccaacg ccgcgcatta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BuphA.01673.n.A2.PW35350

PURIFICATION DATe:	4/11/2012
CONCENTRATION:	30.52mg/ml
OBSERVED MW:	57kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	99

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAGIHSDAK TTEPQQYATQ QSIPAESGHW PAANWADQFG DAQLKALIDE ALKGSPTLEQ ARARVASASA YSETAKASTM PRVDADYSLT RQQYSGTALV PPPTGGSWQT ENKGLLSASY DLDLWGKNRE ALKAAISQLQ ASQADAEVVK LTLTTSIART YNQLARLYAL RDIAQQEIVQ REQIDRITAG RIATGLDTEV ERKTAQANLA TSRATLKSLD GQILATRYQI AALLGAGPDR GLQIARPTLG IGDDVNLPDN LPADLVSRRP DIVAARWRVD AMTHDVKEAK AEFYPDINLS AAIGLDAFGF GRFLTAASRT ASVGPAIHLP IFDAGELRAQ LKGRYADFDY AVATYNQTLV TALSEVATQL AGVRSTDGQL VDAQTAQDAA RQADSLALVQ YKAGLTNQLT VLNADVNALA ADQAVANLRM DRRDQQIALA SALGGGFVDT SNTDQNAHVT SAPETSANAA H

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGGGCATT CATAGCGATG CGAAGACGAC CGAACCGCAG CAGTACGCGA CGCAGCAAAG CATTCCCGCC GAGTCGGGCC ATTGGCCTGC CGCCAATTGG GCCGATCAGT TCGGCGACGC GCAACTGAAG GCGCTGATCG ACGAGGCACT GAAGGGCAGT CCGACGCTTG AACAGGCCCG CGCCCGTGTG GCATCGGCGT CGGCGTATAG CGAAACGGCG AAGGCAAGCA CGATGCCGCG CGTCGACGCC GACTATTCGC TTACGCGTCA GCAGTATTCG GGCACCGCGC TCGTGCCGCC GCCGACGGGT GGCTCGTGGC AGACGGAGAA CAAGGGTCTG CTGAGCGCTT CGTACGATCT CGACCTGTGG GGCAAGAACC GCGAAGCGCT GAAGGCGGCG ATCTCGCAAC TGCAGGCGAG CCAGGCCGAT GCCGAAGTCG TCAAGCTCAC GCTGACGACT TCGATTGCGC GCACGTACAA CCAGCTTGCG CGTCTGTATG CATTGCGCGA TATCGCGCAG CAGGAGATCG TACAGCGCGA ACAGATCGAC AGAATTACGG CGGGCCGCAT CGCGACGGGC CTCGATACGG AAGTCGAGCG CAAGACGGCT CAGGCGAATC TCGCGACGAG CCGCGCCACG TTGAAGTCGC TCGACGGTCA GATTCTTGCC ACGCGATATC AGATCGCCGC GCTGCTCGGC GCTGGCCCGG ACCGTGGACT GCAGATCGCG CGTCCCACGC TCGGCATCGG CGACGACGTG AATCTGCCCG ACAACCTCCC CGCGGATCTG GTAAGCCGCC GTCCCGACAT CGTCGCCGCA CGCTGGCGCG TCGATGCAAT GACGCACGAC GTAAAGGAGG CGAAAGCCGA GTTCTATCCC GACATCAACC TGAGCGCGGC GATCGGACTC GACGCGTTCG GTTTCGGCCG ATTCTTGACG GCGGCGAGCC GCACGGCATC CGTCGGTCCC GCGATACATC TGCCCATCTT CGATGCCGGC GAACTACGCG CGCAACTGAA GGGCCGTTAC GCCGATTTCG ACTATGCCGT CGCAACCTAC AACCAGACGC TCGTTACCGC GTTGAGCGAG GTTGCCACGC AACTTGCCGG CGTGCGTTCG ACGGACGGCC AACTCGTCGA CGCGCAAACC GCACAGGACG CCGCGCGCCA GGCCGATTCG CTCGCACTCG TGCAATACAA GGCCGGGCTC ACGAACCAGC TGACGGTATT GAATGCCGAT GTGAATGCGC TTGCCGCCGA TCAGGCCGTT GCGAACCTGC GAATGGACCG CCGCGATCAG CAGATCGCGC TGGCATCCGC GCTGGGCGGC GGCTTCGTCG ATACCTCGAA CACGGACCAG AACGCGCACG TCACGTCCGC CCCTGAAACG TCTGCCAACG CCGCGCATTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A