BuphA.01673.j

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuphA.01673.j
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01673.j.A2.GE34742 Mature protein( BuphA.01673.j ) 20 503
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3687
RefSeq: YP_001859685.1
UniProt: B2JLK6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNRTILTALA AALFASGCTL APTYERPAAP VAATYPQGDV YDNQPAKGAA TRTANDHAAI DIGWREFFID PRLQRLIELA LQNNRDLRVS ALKVEEARAQ YRIQRADLMP SVSVDGTGTR SRTPKDLSYV GRATVGSDYQ VAASASWELD FFGKVRSLTE QSMQTYFSTA QARKAEEILL VSQVADQYLT LVAYGEMLAS TKNTYDTARR SYELTKLQYD MGTGSELDLR EAAGTMQQAQ ANYESQQRSR AQAENALVLL VGAPLAADLP QGLPLASQQL LADIPAGLPS DLLTRRPDIM EAEATLIGAN ANIGAARAAF FPSISLTGDF GTASASLGTL FKPGQLAWSF GPTISVPIFK GGENRANLDL AKLEKNVAVA QYEKAIQTAF REVSDGLAAR GTYDRQIKAL QENVESQQRR YDLSDVRYRN GADSYLNVLT AQTDLYSAQQ SLITASLNRL TNLVDLYQYL GGGWIEHSGD TPRAADAPYA QQTEPTTHVA SQP
NT Sequence
ATGAACAGAA CGATACTGAC GGCCCTCGCG GCCGCGCTCT TCGCGAGCGG CTGCACGCTC GCGCCGACTT ATGAACGGCC GGCCGCGCCT GTCGCGGCGA CCTATCCGCA AGGCGACGTC TACGATAACC AGCCTGCAAA AGGCGCGGCC ACGCGCACGG CCAACGATCA CGCCGCCATT GACATCGGCT GGCGCGAATT CTTCATCGAT CCGCGCCTGC AACGTCTGAT CGAACTCGCG TTGCAGAACA ACCGCGACTT GCGCGTGTCG GCGCTCAAGG TCGAGGAAGC GCGTGCGCAA TACCGCATCC AGCGCGCCGA CCTGATGCCG TCGGTTTCCG TCGACGGCAC GGGCACGCGC AGCCGCACGC CGAAGGATCT GTCGTATGTC GGACGGGCGA CGGTCGGCAG CGACTATCAG GTGGCGGCGA GCGCGTCATG GGAACTCGAC TTCTTCGGCA AGGTGCGCAG CCTCACCGAG CAGTCCATGC AGACGTACTT CTCGACAGCG CAGGCGCGCA AGGCGGAAGA GATCCTGCTG GTGTCGCAGG TCGCCGATCA ATATCTGACG CTCGTCGCCT ACGGCGAAAT GCTGGCAAGC ACGAAGAACA CCTACGATAC GGCGCGCCGC TCGTATGAGC TGACGAAGCT CCAGTACGAC ATGGGCACCG GCTCCGAACT CGATCTGCGC GAAGCGGCGG GCACGATGCA GCAGGCGCAG GCGAACTACG AAAGCCAGCA GCGTTCGCGC GCGCAGGCCG AGAACGCGCT CGTGCTGCTG GTCGGCGCGC CGTTGGCCGC CGACTTGCCG CAAGGCTTGC CGCTCGCGAG CCAGCAACTG CTCGCGGACA TTCCGGCGGG CTTGCCGTCC GACCTGCTCA CGCGCCGTCC CGACATCATG GAAGCCGAGG CGACGCTGAT CGGCGCGAAC GCGAATATCG GTGCGGCGCG TGCGGCGTTC TTCCCGAGCA TCTCGCTGAC GGGCGACTTC GGTACGGCAA GCGCGAGTCT CGGCACGCTG TTCAAACCGG GCCAGCTCGC ATGGAGTTTC GGCCCGACCA TTTCCGTGCC GATCTTCAAG GGCGGCGAGA ACCGCGCGAA TCTCGATCTC GCGAAGCTCG AAAAGAACGT TGCCGTCGCA CAGTACGAGA AAGCGATCCA GACGGCGTTC CGCGAAGTCT CCGACGGTCT CGCCGCGCGC GGCACGTACG ACCGGCAGAT CAAGGCGCTG CAGGAGAACG TCGAGTCGCA GCAGCGTCGC TATGATCTGT CGGATGTGCG CTATCGCAAT GGGGCCGACA GCTATCTGAA CGTGCTGACC GCGCAGACCG ATCTGTATTC GGCGCAACAG TCGCTGATTA CGGCATCGTT GAACCGCCTG ACTAATCTTG TCGATCTGTA TCAGTACCTG GGCGGCGGCT GGATCGAGCA TTCGGGCGAC ACGCCCCGTG CCGCCGACGC GCCATACGCA CAGCAGACGG AACCGACCAC ACACGTCGCT TCCCAGCCG
Details for BuphA.01673.j.A2.GE34742
HARVESTED ON: 3/13/2012
SEQUENCED ON: 3/13/2012
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MLAPTYERPA APVAATYPQG DVYDNQPAKG AATRTANDHA AIDIGWREFF IDPRLQRLIE LALQNNRDLR VSALKVEEAR AQYRIQRADL MPSVSVDGTG TRSRTPKDLS YVGRATVGSD YQVAASASWE LDFFGKVRSL TEQSMQTYFS TAXARKAEEI LLVSQVADQY LTLVAYXEML ASTKNTYDTA RRSYELTKLX YDMGTGSELD LREAAGTMQQ AQANYESQQR SRAQAENALV LLVGAPLAAD LPQGLPLASQ QLLADIPAGL PSDLLTRRPD IMEAEATLIG ANANIGAARA AFFPSISLTG DFGTASASLG TLFKPGQLAW SFGPTISVPI FKGGENRANL DLAKLEKNVA VAQYEKAIQT AFREVSDGLA ARGTYDRQIK ALQENVESQQ RRYDLSDVRY RNGADSYLNV LTAQTDLYSA QQSLITASLN RLTNLVDLYQ YLGGGWIEHS GDTPRAADAP YAQQTEPTTH VA
Validated NT Sequence
gaagcgacgt gtgtggtcgg ttccgtctgc tgtgcgtatg gcgcgtcggc ggcacggggc gtgtcgcccg aatgctcgat ccagccgccg cccaggtact gatacagatc gacaagatta gtcaggcggt tcaacgatgc cgtaatcagc gactgttgcg ccgaatacag atcggtctgc gcggtcagca cgttcagata gctgtcggcc ccattgcgat agcgcacatc cgacagatca tagcgacgct gctgcgactc gacgttctcc tgcagcgcct tgatctgccg gtcgtacgtg ccgcgcgcgg cgagaccgtc ggagacttcg cggaacgccg tctggatcgc tttctcgtac tgtgcgacgg caacgttctt ttcgagcttc gcgagatcga gattcgcgcg gttctcgccg cccttgaaga tcggcacgga aatggtcggg ccgaaactcc atgcgagctg gcccggtttg aacagcgtgc cgagactcgc gcttgccgta ccgaagtcgc ccgtcagcga gatgctcggg aagaacgccg cacgcgccgc accgatattc gcgttcgcgc cgatcagcgt cgcctcggct tccatgatgt cgggacggcg cgtgagcagg tcggacggca agcccgccgg aatgtccgcg agcagttgct ggctcgcgag cggcaagcct tgcggcaagt cggcggccaa cggcgcgccg accagcagca cgagcgcgtt ctcggcctgc gcgcgcgaac gctgctggct ttcgtagttc gcctgcgcct gctgcatcgt gcccgccgct tcgcgcagat cgagttcnga gccggtgccc atgtcgtacn gnagcttcgt cagctcatac gagcggcgcg ccgtatcgta ggtgttcttc gtgcttgcca gcatttcgcn gtaggcgacg agcgtcagat attgatcggc gacctgcgac accagcagga tctcttccgc cttgcgcgcn tgcgctgtcg agaagtacgt ctgcatggac tgctcggtga ggctgcgcac cttgccgaag aagtcgagtt cccatgacgc gctcgccgcc acctgatagt cgctgccgac cgtcgcccgt ccgacatacg acagatcctt cggcgtgcgg ctgcgcgtgc ccgtgccgtc gacggaaacc gacggcatca ggtcggcgcg ctggatgcgg tattgcgcac gcgcttcctc gaccttgagc gccgacacgc gcaagtcgcg gttgttctgc aacgcgagtt cgatcagacg ttgcaggcgc ggatcgatga agaattcgcg ccagccgatg tcaatggcgg cgtgatcgtt ggccgtgcgc gtggccgcgc cttttgcagg ctggttatcg tagacgtcgc cttgcggata ggtcgccgcg acaggcgcgg ccggccgttc ataagtcggc gcgagcatcg aaccaggacc ctgggtctga gcttcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLAPTYERP AAPVAATYPQ GDVYDNQPAK GAATRTANDH AAIDIGWREF FIDPRLQRLI ELALQNNRDL RVSALKVEEA RAQYRIQRAD LMPSVSVDGT GTRSRTPKDL SYVGRATVGS DYQVAASASW ELDFFGKVRS LTEQSMQTYF STAQARKAEE ILLVSQVADQ YLTLVAYGEM LASTKNTYDT ARRSYELTKL QYDMGTGSEL DLREAAGTMQ QAQANYESQQ RSRAQAENAL VLLVGAPLAA DLPQGLPLAS QQLLADIPAG LPSDLLTRRP DIMEAEATLI GANANIGAAR AAFFPSISLT GDFGTASASL GTLFKPGQLA WSFGPTISVP IFKGGENRAN LDLAKLEKNV AVAQYEKAIQ TAFREVSDGL AARGTYDRQI KALQENVESQ QRRYDLSDVR YRNGADSYLN VLTAQTDLYS AQQSLITASL NRLTNLVDLY QYLGGGWIEH SGDTPRAADA PYAQQTEPTT HVASQP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcgcgccg acttatgaac ggccggccgc gcctgtcgcg gcgacctatc cgcaaggcga cgtctacgat aaccagcctg caaaaggcgc ggccacgcgc acggccaacg atcacgccgc cattgacatc ggctggcgcg aattcttcat cgatccgcgc ctgcaacgtc tgatcgaact cgcgttgcag aacaaccgcg acttgcgcgt gtcggcgctc aaggtcgagg aagcgcgtgc gcaataccgc atccagcgcg ccgacctgat gccgtcggtt tccgtcgacg gcacgggcac gcgcagccgc acgccgaagg atctgtcgta tgtcggacgg gcgacggtcg gcagcgacta tcaggtggcg gcgagcgcgt catgggaact cgacttcttc ggcaaggtgc gcagcctcac cgagcagtcc atgcagacgt acttctcgac agcgcaggcg cgcaaggcgg aagagatcct gctggtgtcg caggtcgccg atcaatatct gacgctcgtc gcctacggcg aaatgctggc aagcacgaag aacacctacg atacggcgcg ccgctcgtat gagctgacga agctccagta cgacatgggc accggctccg aactcgatct gcgcgaagcg gcgggcacga tgcagcaggc gcaggcgaac tacgaaagcc agcagcgttc gcgcgcgcag gccgagaacg cgctcgtgct gctggtcggc gcgccgttgg ccgccgactt gccgcaaggc ttgccgctcg cgagccagca actgctcgcg gacattccgg cgggcttgcc gtccgacctg ctcacgcgcc gtcccgacat catggaagcc gaggcgacgc tgatcggcgc gaacgcgaat atcggtgcgg cgcgtgcggc gttcttcccg agcatctcgc tgacgggcga cttcggtacg gcaagcgcga gtctcggcac gctgttcaaa ccgggccagc tcgcatggag tttcggcccg accatttccg tgccgatctt caagggcggc gagaaccgcg cgaatctcga tctcgcgaag ctcgaaaaga acgttgccgt cgcacagtac gagaaagcga tccagacggc gttccgcgaa gtctccgacg gtctcgccgc gcgcggcacg tacgaccggc agatcaaggc gctgcaggag aacgtcgagt cgcagcagcg tcgctatgat ctgtcggatg tgcgctatcg caatggggcc gacagctatc tgaacgtgct gaccgcgcag accgatctgt attcggcgca acagtcgctg attacggcat cgttgaaccg cctgactaat cttgtcgatc tgtatcagta cctgggcggc ggctggatcg agcattcggg cgacacgccc cgtgccgccg acgcgccata cgcacagcag acggaaccga ccacacacgt cgcttcccag ccgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa