BuphA.01673.i

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuphA.01673.i
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01673.i.A2.GE32193 Mature protein( BuphA.01673.i ) 29 500
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3682
RefSeq: YP_001859681.1
UniProt: B2JLK2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMDKATRTTR RVQRSIVAAA IATLCAGCAV GPDYKRPDVV VPAAFKEANS SDAGDVWQQA KPDPAASLDS RWWTVFGDGT LDDLCERALK ANQTLAQYEA AYRSARAQVA SSRASLFPTV SFAGSGTRSR TGTGSNTTPI SSGSSTSSYA SKSVSAELEA SWEPDLWGSV RRSVEVSEAS AQASDAQLAG ERLSVLATLA VDYFTVRAAD ADLAILEEER RIDAELLALT EAKYKQGVSS YDDVRTAHNT LQAIDESIAS AQLTRRQYEH AIAVLLGEPP AAFSLPVQAD YRFDLPALPV ALPSTLLQRR PDVVQAERTV AQYNAKIGVA KAGYFPTITL TADGGWSGTS FAHLISLPTR FWSLGLDVAQ TVFDAGATSA SVRAARANYD QEVAAYRQTV LSAFQDVEDY LSAVQIASRQ AAASNEVARR SGELAASQQR NFAAGTASRI DMLDTQLTQV EDRKIALDYS STTLQNAVLL VKALGGGWDS QLKTADSSGR
NT Sequence
ATGATGGACA AAGCTACGCG CACGACGCGG CGTGTGCAAC GATCGATCGT GGCTGCCGCC ATTGCAACGC TGTGCGCAGG CTGCGCGGTC GGCCCCGACT ACAAGCGGCC CGATGTCGTC GTGCCCGCCG CCTTCAAGGA AGCCAACAGT TCCGATGCGG GCGACGTGTG GCAGCAGGCG AAGCCCGATC CCGCCGCGTC ACTCGATAGC CGCTGGTGGA CCGTGTTCGG CGACGGGACG CTCGACGATC TGTGCGAGCG CGCGCTGAAG GCGAACCAGA CCCTCGCGCA ATACGAAGCC GCGTATCGCT CGGCGCGCGC CCAGGTCGCG TCGTCGCGCG CGAGTCTGTT TCCCACGGTG TCGTTCGCCG GTTCGGGTAC GCGCTCGCGT ACGGGTACCG GCAGCAACAC GACACCGATT TCATCAGGCA GCAGCACTAG CAGCTACGCG TCGAAGAGCG TGTCGGCGGA ACTGGAGGCG AGCTGGGAGC CGGACCTGTG GGGCAGCGTG CGGCGCTCCG TCGAAGTGAG CGAGGCTAGC GCGCAGGCAT CGGATGCGCA ACTGGCGGGC GAGCGCCTGA GCGTGCTCGC GACGCTGGCC GTCGACTACT TCACCGTGCG GGCCGCCGAC GCCGATCTCG CGATTCTCGA AGAGGAACGC CGCATCGATG CGGAACTGCT CGCGCTGACG GAAGCGAAGT ACAAGCAAGG CGTTTCCTCG TATGACGATG TGCGCACGGC CCACAACACA TTGCAGGCGA TCGACGAAAG TATCGCGAGC GCGCAACTCA CGCGCCGTCA GTACGAGCAT GCGATTGCCG TGCTGCTCGG CGAGCCGCCC GCGGCGTTTT CGCTGCCGGT TCAGGCGGAC TATCGGTTCG ACTTGCCGGC GCTGCCCGTC GCGTTGCCGT CCACATTGCT GCAGCGGCGG CCGGACGTCG TGCAGGCCGA GCGCACGGTC GCGCAATACA ACGCGAAAAT CGGCGTGGCG AAGGCGGGCT ATTTCCCGAC CATCACGCTA ACGGCCGACG GCGGCTGGAG CGGCACGTCG TTCGCGCATC TAATCTCGCT GCCCACGCGC TTCTGGTCGC TCGGACTCGA CGTTGCGCAG ACGGTGTTCG ATGCGGGCGC GACGAGCGCC TCGGTGCGTG CCGCGCGCGC CAACTACGAT CAGGAAGTGG CCGCGTACCG GCAGACCGTG CTGAGCGCGT TCCAGGACGT TGAAGATTAT CTGAGCGCAG TGCAGATCGC GTCGCGCCAG GCGGCAGCGT CGAACGAGGT CGCGCGGCGC AGCGGCGAAC TGGCCGCCAG CCAGCAACGC AACTTCGCGG CAGGCACGGC GAGCCGCATC GACATGCTCG ACACGCAACT CACGCAAGTG GAGGACCGCA AGATCGCCCT CGACTACAGC AGCACGACGT TGCAGAACGC TGTGCTGCTA GTGAAAGCGC TGGGGGGCGG GTGGGACAGT CAACTGAAGA CGGCGGATTC TTCCGGGAGG
Details for BuphA.01673.i.A2.GE32193
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 13
PERCENT COVERAGE: 45
Validated AA Sequence
MFXXTPXXXP XXMTXXXXXX XXXXGXXXGX RXXXXXXXXX XXXXXXXXXX XMRXXXEXXX XXAXXXXXXP MEDXXXXXDA XXXVXXXAXX XXHPRXXRXR VSXXAXLXXX XXXXXVSXXV VPXXXXAXGX GXXXXXXXXX XXXXXXXXXG XXXXXXXXGX XXXXXXXXXX XXXXXAQAXD AXLAGEXXXX LXTLXXDYFT CGXXTPISLF XKRNAASMRX CSR
Validated NT Sequence
gcggnncagc catgtttann nnnactccnn nnnnnnancc nnnnnnnatg acnannnnnn nncannanna nnnaanccan nggggctgnn nnnnnggngn ncgcnncngn nnncnancng ncngntgnnn nnnnnnngnn ncnntcnnnn nnnnnanacn gcngatgcgn nnnncnnggg agnatgnnnn gnctgntgcc nccncnnacn nncnntnncc natggaggac cnngnnnnnn gacnggacgc tcnannatnn gtnnnannnn nngctganng nnanncacac cctcgcgnnn nacgaanccg cgtatcgntn nnngcgnnnc tnngtncngn ngnnnnnngn nnnnntgttt cnnnnngngt cgttccnnng ttnnnnnacg cgntcgggnn gggnnnnnng nnnntnntnn ngnannnnnn ctannagttn nncnncnaag annntgncgg nnnnnnntgn tgnnanntgn nagncggann nnnannnnnn nnntnncncc nnnnacnnna annnancnan nnnngcgcgc aggcnnnnga tgcncnnctg gcgggngagn nnntnancgn gctcnngacn ctgnncnncg actacttcac ntgcgggnng ncgacnccga tctcnctatt cnncaagagg aacgccgcat cgatgcgnan ntgctcncgc tgacnnnnng aannannnnc aaggcgnttn cntcgnatga nnnntngcgn anngnccacn nnannttgna ngcnnncnan natannntcc ncnagnnnnn nnntnnnnnn nnnnnnta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVGPDYKR PDVVVPAAFK EANSSDAGDV WQQAKPDPAA SLDSRWWTVF GDGTLDDLCE RALKANQTLA QYEAAYRSAR AQVASSRASL FPTVSFAGSG TRSRTGTGSN TTPISSGSST SSYASKSVSA ELEASWEPDL WGSVRRSVEV SEASAQASDA QLAGERLSVL ATLAVDYFTV RAADADLAIL EEERRIDAEL LALTEAKYKQ GVSSYDDVRT AHNTLQAIDE SIASAQLTRR QYEHAIAVLL GEPPAAFSLP VQADYRFDLP ALPVALPSTL LQRRPDVVQA ERTVAQYNAK IGVAKAGYFP TITLTADGGW SGTSFAHLIS LPTRFWSLGL DVAQTVFDAG ATSASVRAAR ANYDQEVAAY RQTVLSAFQD VEDYLSAVQI ASRQAAASNE VARRSGELAA SQQRNFAAGT ASRIDMLDTQ LTQVEDRKIA LDYSSTTLQN AVLLVKALGG GWDSQLKTAD SSGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggtcggc cccgactaca agcggcccga tgtcgtcgtg cccgccgcct tcaaggaagc caacagttcc gatgcgggcg acgtgtggca gcaggcgaag cccgatcccg ccgcgtcact cgatagccgc tggtggaccg tgttcggcga cgggacgctc gacgatctgt gcgagcgcgc gctgaaggcg aaccagaccc tcgcgcaata cgaagccgcg tatcgctcgg cgcgcgccca ggtcgcgtcg tcgcgcgcga gtctgtttcc cacggtgtcg ttcgccggtt cgggtacgcg ctcgcgtacg ggtaccggca gcaacacgac accgatttca tcaggcagca gcactagcag ctacgcgtcg aagagcgtgt cggcggaact ggaggcgagc tgggagccgg acctgtgggg cagcgtgcgg cgctccgtcg aagtgagcga ggctagcgcg caggcatcgg atgcgcaact ggcgggcgag cgcctgagcg tgctcgcgac gctggccgtc gactacttca ccgtgcgggc cgccgacgcc gatctcgcga ttctcgaaga ggaacgccgc atcgatgcgg aactgctcgc gctgacggaa gcgaagtaca agcaaggcgt ttcctcgtat gacgatgtgc gcacggccca caacacattg caggcgatcg acgaaagtat cgcgagcgcg caactcacgc gccgtcagta cgagcatgcg attgccgtgc tgctcggcga gccgcccgcg gcgttttcgc tgccggttca ggcggactat cggttcgact tgccggcgct gcccgtcgcg ttgccgtcca cattgctgca gcggcggccg gacgtcgtgc aggccgagcg cacggtcgcg caatacaacg cgaaaatcgg cgtggcgaag gcgggctatt tcccgaccat cacgctaacg gccgacggcg gctggagcgg cacgtcgttc gcgcatctaa tctcgctgcc cacgcgcttc tggtcgctcg gactcgacgt tgcgcagacg gtgttcgatg cgggcgcgac gagcgcctcg gtgcgtgccg cgcgcgccaa ctacgatcag gaagtggccg cgtaccggca gaccgtgctg agcgcgttcc aggacgttga agattatctg agcgcagtgc agatcgcgtc gcgccaggcg gcagcgtcga acgaggtcgc gcggcgcagc ggcgaactgg ccgccagcca gcaacgcaac ttcgcggcag gcacggcgag ccgcatcgac atgctcgaca cgcaactcac gcaagtggag gaccgcaaga tcgccctcga ctacagcagc acgacgttgc agaacgctgt gctgctagtg aaagcgctgg ggggcgggtg ggacagtcaa ctgaagacgg cggattcttc cgggaggtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa