BuphA.01673.g

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuphA.01673.g
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01673.g.A1.GE32300 Full length( BuphA.01673.g ) 1 494
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.3549
RefSeq: YP_001859561.1
UniProt: B2JKY6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLFDLGGAA RRRRSHVGAM LAPVPAAVTV LLAGCTVGPD YKGAPATTHV SSFKRAPDGV VQTAPGVAAW WLALGDPQLT ALIDAGIANS PDLRAAQARV RQARAGLSQQ QSNELPKVGA SAAYLRTREP DLSSLSGGSS SSGGRGPIAL YLAGFDASWE LDLFGGTRRA VEAASAQADA SQAELADAHV QLAAEIGSAY VGLRDQQQSM AIVRKSAELE ARVLTLTEQR RDRGVASQLD VERVRTQVEN TRNALIPLDA QIAESLDELA VLTGREPGAL DDELNASAPL PALPQTIAVG DPAQLLRRRP DVRAAERRLA SQSAQIGERE ADWFPKVTLF GSLAFSAADP GHLVRKNNFT WLAVPYLQWN AFDFGRTKAR VDQSRAALDE AQAKYESTVL GALRDADVAL SRYGHERENV VSLRTVESSA TRAATLTEQR YRAGTATALD WLDAERTRYT AEQNRIQSDA QLIKDYVALQ KSLGLGWEGM VSTQ
NT Sequence
ATGAAGCTAT TTGATCTGGG CGGCGCCGCG CGCCGCCGTC GTTCGCATGT TGGCGCGATG CTTGCGCCCG TCCCGGCTGC CGTGACCGTG CTGCTTGCAG GCTGCACGGT GGGTCCCGAC TACAAGGGCG CGCCCGCCAC GACGCACGTG AGCAGCTTCA AGCGTGCGCC GGACGGCGTG GTGCAGACGG CGCCCGGCGT CGCGGCATGG TGGCTCGCGC TCGGCGATCC GCAACTGACG GCGCTGATCG ACGCGGGCAT CGCCAACAGC CCCGATCTGC GTGCCGCGCA GGCGCGCGTG CGGCAGGCGC GCGCGGGCTT GTCCCAGCAG CAGAGCAACG AGCTGCCGAA GGTCGGCGCG AGCGCGGCGT ATCTGCGCAC GCGCGAACCT GATCTGTCGT CGCTGAGCGG CGGTTCCAGC TCGTCGGGCG GACGCGGACC CATTGCGCTG TATCTCGCGG GGTTCGACGC GAGCTGGGAA CTCGATTTGT TCGGCGGCAC GCGCCGTGCG GTCGAAGCGG CCTCGGCGCA AGCAGATGCG TCGCAGGCAG AACTCGCCGA CGCGCACGTG CAGCTCGCCG CGGAAATCGG CAGCGCTTAT GTGGGCTTGC GCGATCAGCA GCAGAGCATG GCGATCGTGC GCAAGTCGGC GGAACTCGAA GCGCGCGTGC TGACGCTGAC CGAGCAGCGG CGCGATCGCG GCGTCGCGTC GCAACTGGAT GTGGAACGCG TGCGCACGCA GGTCGAAAAC ACGCGCAATG CGCTGATTCC CCTTGACGCG CAGATCGCCG AATCGCTCGA TGAACTCGCC GTGCTGACAG GCCGCGAGCC GGGCGCGCTG GACGACGAAC TGAACGCGTC GGCGCCACTG CCCGCGTTGC CACAGACGAT CGCCGTGGGC GATCCCGCGC AACTGCTGAG GCGACGTCCC GATGTGCGCG CAGCGGAGCG GCGACTCGCG TCGCAGAGCG CGCAGATCGG CGAGCGCGAA GCGGACTGGT TTCCCAAAGT GACCCTGTTC GGCAGCCTGG CCTTCAGCGC CGCCGACCCC GGCCACCTGG TGCGCAAGAA TAATTTCACG TGGCTCGCGG TGCCGTATCT GCAATGGAAT GCGTTCGACT TCGGCCGCAC GAAGGCACGT GTCGATCAAT CGCGTGCCGC GCTCGACGAG GCGCAGGCCA AGTACGAAAG CACGGTGCTC GGCGCGCTGC GCGATGCCGA TGTCGCGCTT TCGCGCTATG GTCATGAGCG TGAGAACGTC GTCAGCCTGC GCACGGTCGA GTCGTCCGCG ACGCGCGCGG CGACGCTGAC GGAACAGCGC TATCGCGCGG GCACGGCGAC GGCGCTCGAC TGGCTCGATG CAGAACGCAC GCGCTATACG GCCGAACAGA ATCGCATCCA GAGCGATGCG CAACTGATCA AGGACTACGT CGCGTTGCAG AAGAGCCTCG GGCTCGGGTG GGAAGGGATG GTGTCCACGC AA
Details for BuphA.01673.g.A1.GE32300
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 55kDa
OBSERVED MW: 55kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 75
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKLFDLGGA ARRRRSHVGA MLAPVPAAVT VLLAGCTVGP DYKGAPATTH VSSFKRAPDG VVQTAPGVAA WWLALGDPQL TALIDAGIAN SPDLRAAQAR VRQARAGLSQ QQSNELPKVG ASAAYLRTRE PDLSSLSGGS SSSGGRGPIA LYLAGFDASW ELDLFGGTRR AVEAASAQAD ASQAELADAH VQLAAEIGSA YVGLRDQQQS MAIVRKSAEL EARVLTLTEQ RRDRGVASQL DVERVRTXVE NTRNALIPLD AQIAESLDEL AVLTGREPGA LDDELNASAX LPALXQTIAV GDPAQLLXDX XMXAQXXXDS XXXXXXSXSX XXXXXXTXXX XXXNPXXXXX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
ttntnnnntt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgaagctat ttgatctggg cggcgccgcg cgccgccgtc gttcgcatgt tggcgcgatg cttgcgcccg tcccggctgc cgtgaccgtg ctgcttgcag gctgcacggt gggtcccgac tacaagggcg cgcccgccac gacgcacgtg agcagcttca agcgtgcgcc ggacggcgtg gtgcagacgg cgcccggcgt cgcggcatgg tggctcgcgc tcggcgatcc gcaactgacg gcgctgatcg acgcgggcat cgccaacagc cccgatctgc gtgccgcgca ggcgcgcgtg cggcaggcgc gcgcgggctt gtcccagcag cagagcaacg agctgccgaa ggtcggcgcg agcgcggcgt atctgcgcac gcgcgaacct gatctgtcgt cgctgagcgg cggttccagc tcgtcgggcg gacgcggacc cattgcgctg tatctcgcgg ggttcgacgc gagctgggaa ctcgatttgt tcggcggcac gcgccgtgcg gtcgaagcgg cctcggcgca agcagatgcg tcgcaggcag aactcgccga cgcgcacgtg cagctcgccg cggaaatcgg cagcgcttat gtgggcttgc gcgatcagca gcagagcatg gcgatcgtgc gcaagtcggc ggaactcgaa gcgcgcgtgc tgacgctgac cgagcagcgg cgcgatcgcg gcgtcgcgtc gcaactggat gtggaacgcg tgcgcacgca ngtcgaaaac acgcgcaatg cgctgattcc ccttgacgcg cagatcgccg aatcgctcga tgaactcgcc gtgctgacag gccgcgagcc gggcgcgctg gacgacgaac tgaacgcgtc ggcgcnnctg cccgcgttgc nncagacgat cgccgtgggc gatcccgcnc aactgctgnn ngacnnccng atgnncgcgc agcnnancnn ngactcnnnn nnnnnancnn gcanatcgnn nagcaannnn nnnnnntncn nnnnnacnnn nnnnnnnnnn nnnnnnaacc cngnnnncnn nngnnnnnna nnnntnnncn nnnnnnnnng nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnannnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLFDLGGA ARRRRSHVGA MLAPVPAAVT VLLAGCTVGP DYKGAPATTH VSSFKRAPDG VVQTAPGVAA WWLALGDPQL TALIDAGIAN SPDLRAAQAR VRQARAGLSQ QQSNELPKVG ASAAYLRTRE PDLSSLSGGS SSSGGRGPIA LYLAGFDASW ELDLFGGTRR AVEAASAQAD ASQAELADAH VQLAAEIGSA YVGLRDQQQS MAIVRKSAEL EARVLTLTEQ RRDRGVASQL DVERVRTQVE NTRNALIPLD AQIAESLDEL AVLTGREPGA LDDELNASAP LPALPQTIAV GDPAQLLRRR PDVRAAERRL ASQSAQIGER EADWFPKVTL FGSLAFSAAD PGHLVRKNNF TWLAVPYLQW NAFDFGRTKA RVDQSRAALD EAQAKYESTV LGALRDADVA LSRYGHEREN VVSLRTVESS ATRAATLTEQ RYRAGTATAL DWLDAERTRY TAEQNRIQSD AQLIKDYVAL QKSLGLGWEG MVSTQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagctattt gatctgggcg gcgccgcgcg ccgccgtcgt tcgcatgttg gcgcgatgct tgcgcccgtc ccggctgccg tgaccgtgct gcttgcaggc tgcacggtgg gtcccgacta caagggcgcg cccgccacga cgcacgtgag cagcttcaag cgtgcgccgg acggcgtggt gcagacggcg cccggcgtcg cggcatggtg gctcgcgctc ggcgatccgc aactgacggc gctgatcgac gcgggcatcg ccaacagccc cgatctgcgt gccgcgcagg cgcgcgtgcg gcaggcgcgc gcgggcttgt cccagcagca gagcaacgag ctgccgaagg tcggcgcgag cgcggcgtat ctgcgcacgc gcgaacctga tctgtcgtcg ctgagcggcg gttccagctc gtcgggcgga cgcggaccca ttgcgctgta tctcgcgggg ttcgacgcga gctgggaact cgatttgttc ggcggcacgc gccgtgcggt cgaagcggcc tcggcgcaag cagatgcgtc gcaggcagaa ctcgccgacg cgcacgtgca gctcgccgcg gaaatcggca gcgcttatgt gggcttgcgc gatcagcagc agagcatggc gatcgtgcgc aagtcggcgg aactcgaagc gcgcgtgctg acgctgaccg agcagcggcg cgatcgcggc gtcgcgtcgc aactggatgt ggaacgcgtg cgcacgcagg tcgaaaacac gcgcaatgcg ctgattcccc ttgacgcgca gatcgccgaa tcgctcgatg aactcgccgt gctgacaggc cgcgagccgg gcgcgctgga cgacgaactg aacgcgtcgg cgccactgcc cgcgttgcca cagacgatcg ccgtgggcga tcccgcgcaa ctgctgaggc gacgtcccga tgtgcgcgca gcggagcggc gactcgcgtc gcagagcgcg cagatcggcg agcgcgaagc ggactggttt cccaaagtga ccctgttcgg cagcctggcc ttcagcgccg ccgaccccgg ccacctggtg cgcaagaata atttcacgtg gctcgcggtg ccgtatctgc aatggaatgc gttcgacttc ggccgcacga aggcacgtgt cgatcaatcg cgtgccgcgc tcgacgaggc gcaggccaag tacgaaagca cggtgctcgg cgcgctgcgc gatgccgatg tcgcgctttc gcgctatggt catgagcgtg agaacgtcgt cagcctgcgc acggtcgagt cgtccgcgac gcgcgcggcg acgctgacgg aacagcgcta tcgcgcgggc acggcgacgg cgctcgactg gctcgatgca gaacgcacgc gctatacggc cgaacagaat cgcatccaga gcgatgcgca actgatcaag gactacgtcg cgttgcagaa gagcctcggg ctcgggtggg aagggatggt gtccacgcaa taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa