BuphA.01673.f

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuphA.01673.f
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01673.f.A2.GE32195 Mature protein( BuphA.01673.f ) 39 515
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01673.f.A2.PS01431 Mature protein( BuphA.01673.f ) 39 515

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1842
RefSeq: YP_001857952.1
UniProt: B2JKS9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLHAKSRAM NFLPWPAPAS SHRAAVAAAV AAFALAGCAN YFDLKDDRQI ASTSQFEASQ SLPAQGGQWP ALDWANQFGD PQLPKLIAEA LEGNPSIAQA QARIAKASSY IETSKSALYP KVEGSYSWTR ELYSANALFP PPYGGTWYSE NNVLASASWD LDLWGKNRER LGQAVSQEKA AEADMQQARV TLAASVARTY NSLAQLYALR DIAEREIKNR QTIGAITNDR VRAGLDTNVE RQTANGNIAT SQSNLTELDG QITSTRYELG TLLGKGPDRG LQIAMPVFDP GSAVSLPDNV PADLVSRRPD IVAARWQVEA AMHNVKEAKA EFFPDVNLAA AFGFDAFGWS KFLTSSSRQI QFGPAIHLPI FDAGALRSQL KGRFADFDLD VANYNQTLIG ALSDVATQIS SIRSIDRQAG DAQRALDAST KAYELAVIRY KAGLSPQLQV LTADQNRLAA EQTVTNLKMR RRDLQFALIK ALGGGFDATQ TGLVVPTDTP PASSAAAAPA THAAN
NT Sequence
ATGTCATTAC ACGCAAAGAG TCGAGCGATG AACTTCCTTC CTTGGCCCGC GCCTGCCTCG TCACACCGGG CGGCCGTCGC TGCAGCCGTG GCGGCGTTCG CCCTCGCCGG CTGCGCGAAC TACTTCGATC TCAAGGACGA CAGGCAGATC GCGTCGACCT CGCAGTTCGA GGCGTCACAG AGCCTGCCCG CGCAAGGCGG CCAATGGCCG GCGCTCGACT GGGCGAACCA GTTCGGCGAT CCGCAACTGC CGAAGCTGAT CGCGGAAGCG CTCGAAGGCA ATCCGTCAAT CGCGCAGGCG CAGGCGCGCA TCGCGAAGGC GTCGTCCTAT ATCGAAACCT CGAAGTCGGC GCTCTATCCG AAGGTCGAGG GCAGCTATTC GTGGACCCGC GAGCTGTACT CTGCCAATGC GCTGTTTCCG CCGCCGTACG GCGGCACGTG GTACAGCGAG AACAATGTGC TCGCCAGCGC GTCGTGGGAT CTCGACTTGT GGGGTAAGAA CCGGGAACGG CTCGGCCAGG CTGTGTCGCA GGAAAAAGCT GCCGAAGCCG ACATGCAGCA GGCCCGCGTG ACCCTCGCCG CGTCGGTCGC GCGTACCTAC AACTCGCTCG CGCAGCTGTA CGCGCTGCGC GACATCGCCG AGCGCGAAAT CAAGAACCGC CAGACGATCG GCGCGATCAC GAACGACCGC GTGCGGGCCG GCCTCGACAC GAACGTCGAG CGTCAGACGG CCAATGGCAA CATCGCGACC AGTCAGTCGA ACCTGACGGA GCTGGACGGC CAGATCACGA GCACGCGCTA CGAACTCGGC ACGCTGCTCG GCAAAGGGCC TGACCGCGGC CTGCAGATCG CCATGCCGGT GTTCGATCCC GGCAGCGCCG TTTCGCTGCC CGACAACGTG CCCGCCGACC TCGTGTCGCG CCGGCCGGAC ATCGTCGCCG CGCGCTGGCA GGTCGAAGCC GCGATGCACA ACGTGAAGGA AGCCAAGGCC GAGTTTTTTC CTGACGTGAA TCTCGCGGCC GCGTTCGGCT TCGACGCGTT CGGCTGGAGC AAATTTCTGA CGTCGTCGAG CCGTCAGATC CAGTTCGGGC CGGCCATCCA TCTGCCGATT TTCGATGCCG GCGCGCTGCG CTCGCAGCTC AAGGGCCGCT TCGCCGATTT CGACCTCGAC GTCGCGAACT ACAACCAGAC GCTGATCGGC GCGCTCTCCG ACGTCGCCAC GCAGATTTCG TCGATCCGTT CCATCGACAG GCAGGCGGGC GACGCACAGC GCGCGCTCGA TGCATCGACG AAAGCGTACG AACTCGCCGT GATCCGCTAC AAGGCCGGTT TGTCGCCGCA GTTGCAGGTA CTGACGGCCG ACCAGAACCG CCTCGCTGCC GAGCAGACGG TGACGAACCT CAAGATGCGC CGGCGCGATC TGCAATTCGC GCTGATCAAG GCGCTCGGCG GCGGTTTCGA CGCGACGCAG ACGGGCCTCG TCGTGCCGAC GGATACGCCT CCTGCATCGT CGGCAGCTGC GGCGCCGGCA ACGCACGCGG CGAAC
Details for BuphA.01673.f.A2.GE32195
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 66
PERCENT COVERAGE: 63
Validated AA Sequence
MFXXTPXXXX XXXXXXXXXX XTXGPGSMAN YFDLKDDRQI ASTSQFEASQ SLPAQGGQWP ALDWANQFGD PQLPKLIAEA LEGNPSIAQA QARIAKASSY IETSKSALYP KVEGSYSWTR ELYSANALFP PPYGGTWYSE NNVLASASWD LDLWGKNRER LGQAVSQEKA AEADMQQARV XLAASVARTY XXLAQLYALR DIAEREIKNR XTIGAITNXR XRXGLDTXRR ASDGXXXXXD XSXXPDGAWT XRSXATNXXX CXXXGPXXXT XXXHXXXXNP XXXFXXXXRX PXXXXXXXXD IXXXXXXXXX XXXXXXXX
Validated NT Sequence
ccnnnnnnnn nnnnnnnnnn nnngcggnnc ngccatgttt annnnnactc ctnnnnnnan ccnnannnnn nnacnannnn nnnnnannnn nntnncaaca nnnggtcctg gttcnatggc gaactacttc gatctcaagg acgacaggca gatcgcgtcg acctcgcagt tcgaggcgtc acagagcctg cccgcgcaag gcggccaatg gccggcgctc gactgggcga accagttcgg cgatccgcaa ctgccgaagc tgatcgcgga agcgctcgaa ggcaatccgt caatcgcgca ggcgcaggcg cgcatcgcga aggcgtcgtc ctatatcgaa acctcgaagt cggcgctcta tccgaaggtc gagggcagct attcgtggac ccgcgagctg tactctgcca atgcgctgtt tccgccgccg tacggcggca cgtggtacag cgagaacaat gtgctcgcca gcgcgtcgtg ggatctcgac ttgtggggta agaaccggga acggctcggc caggctgtgt cgcaggaaaa agctgccgaa gccgacatgc agcaggcccg cgtgancctc gccgcgtcgg tcgcgcgtac ntacaantng ctcgcgcagc tgtacgcgct gcgcgacatc gccgagcgcg aaatcaagaa ccgnnnnacg atcggngcga tcacgaacga ncgcgngcgn nnnggcctcg acacgaancg tcgagcgtca gacggcnnan gncnnnntcn cgaccngtcn ntnnaacctg acggagcntg gacggncaga tcnnnngcta cnaactngnn nnnctgctnn nnnnnagggc cnngancgcn gactgnanat cnncatgnnn ggngnncgaa ccccgnnnnn nnnttcnntn ccnannnncg ngnnccnncn nannnnngtc nnnnncnnnn ngacatccnt cnncnnnnnn ngnnannnnc nnanncnncn nnnnnnnnnn tnnaagnaa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMANYFDLKD DRQIASTSQF EASQSLPAQG GQWPALDWAN QFGDPQLPKL IAEALEGNPS IAQAQARIAK ASSYIETSKS ALYPKVEGSY SWTRELYSAN ALFPPPYGGT WYSENNVLAS ASWDLDLWGK NRERLGQAVS QEKAAEADMQ QARVTLAASV ARTYNSLAQL YALRDIAERE IKNRQTIGAI TNDRVRAGLD TNVERQTANG NIATSQSNLT ELDGQITSTR YELGTLLGKG PDRGLQIAMP VFDPGSAVSL PDNVPADLVS RRPDIVAARW QVEAAMHNVK EAKAEFFPDV NLAAAFGFDA FGWSKFLTSS SRQIQFGPAI HLPIFDAGAL RSQLKGRFAD FDLDVANYNQ TLIGALSDVA TQISSIRSID RQAGDAQRAL DASTKAYELA VIRYKAGLSP QLQVLTADQN RLAAEQTVTN LKMRRRDLQF ALIKALGGGF DATQTGLVVP TDTPPASSAA AAPATHAAN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgaactac ttcgatctca aggacgacag gcagatcgcg tcgacctcgc agttcgaggc gtcacagagc ctgcccgcgc aaggcggcca atggccggcg ctcgactggg cgaaccagtt cggcgatccg caactgccga agctgatcgc ggaagcgctc gaaggcaatc cgtcaatcgc gcaggcgcag gcgcgcatcg cgaaggcgtc gtcctatatc gaaacctcga agtcggcgct ctatccgaag gtcgagggca gctattcgtg gacccgcgag ctgtactctg ccaatgcgct gtttccgccg ccgtacggcg gcacgtggta cagcgagaac aatgtgctcg ccagcgcgtc gtgggatctc gacttgtggg gtaagaaccg ggaacggctc ggccaggctg tgtcgcagga aaaagctgcc gaagccgaca tgcagcaggc ccgcgtgacc ctcgccgcgt cggtcgcgcg tacctacaac tcgctcgcgc agctgtacgc gctgcgcgac atcgccgagc gcgaaatcaa gaaccgccag acgatcggcg cgatcacgaa cgaccgcgtg cgggccggcc tcgacacgaa cgtcgagcgt cagacggcca atggcaacat cgcgaccagt cagtcgaacc tgacggagct ggacggccag atcacgagca cgcgctacga actcggcacg ctgctcggca aagggcctga ccgcggcctg cagatcgcca tgccggtgtt cgatcccggc agcgccgttt cgctgcccga caacgtgccc gccgacctcg tgtcgcgccg gccggacatc gtcgccgcgc gctggcaggt cgaagccgcg atgcacaacg tgaaggaagc caaggccgag ttttttcctg acgtgaatct cgcggccgcg ttcggcttcg acgcgttcgg ctggagcaaa tttctgacgt cgtcgagccg tcagatccag ttcgggccgg ccatccatct gccgattttc gatgccggcg cgctgcgctc gcagctcaag ggccgcttcg ccgatttcga cctcgacgtc gcgaactaca accagacgct gatcggcgcg ctctccgacg tcgccacgca gatttcgtcg atccgttcca tcgacaggca ggcgggcgac gcacagcgcg cgctcgatgc atcgacgaaa gcgtacgaac tcgccgtgat ccgctacaag gccggtttgt cgccgcagtt gcaggtactg acggccgacc agaaccgcct cgctgccgag cagacggtga cgaacctcaa gatgcgccgg cgcgatctgc aattcgcgct gatcaaggcg ctcggcggcg gtttcgacgc gacgcagacg ggcctcgtcg tgccgacgga tacgcctcct gcatcgtcgg cagctgcggc gccggcaacg cacgcggcga actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BuphA.01673.f.A2.PS01431
PURIFICATION DATe: 3/26/2012
CONCENTRATION: 18.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 66
PERCENT COVERAGE: 63
Protocol Notes
notes unavailable
Validated AA Sequence
MFXXTPXXXX XXXXXXXXXX XTXGPGSMAN YFDLKDDRQI ASTSQFEASQ SLPAQGGQWP ALDWANQFGD PQLPKLIAEA LEGNPSIAQA QARIAKASSY IETSKSALYP KVEGSYSWTR ELYSANALFP PPYGGTWYSE NNVLASASWD LDLWGKNRER LGQAVSQEKA AEADMQQARV XLAASVARTY XXLAQLYALR DIAEREIKNR XTIGAITNXR XRXGLDTXRR ASDGXXXXXD XSXXPDGAWT XRSXATNXXX CXXXGPXXXT XXXHXXXXNP XXXFXXXXRX PXXXXXXXXD IXXXXXXXXX XXXXXXXX
Validated NT Sequence
ccnnnnnnnn nnnnnnnnnn nnngcggnnc ngccatgttt annnnnactc ctnnnnnnan ccnnannnnn nnacnannnn nnnnnannnn nntnncaaca nnnggtcctg gttcnatggc gaactacttc gatctcaagg acgacaggca gatcgcgtcg acctcgcagt tcgaggcgtc acagagcctg cccgcgcaag gcggccaatg gccggcgctc gactgggcga accagttcgg cgatccgcaa ctgccgaagc tgatcgcgga agcgctcgaa ggcaatccgt caatcgcgca ggcgcaggcg cgcatcgcga aggcgtcgtc ctatatcgaa acctcgaagt cggcgctcta tccgaaggtc gagggcagct attcgtggac ccgcgagctg tactctgcca atgcgctgtt tccgccgccg tacggcggca cgtggtacag cgagaacaat gtgctcgcca gcgcgtcgtg ggatctcgac ttgtggggta agaaccggga acggctcggc caggctgtgt cgcaggaaaa agctgccgaa gccgacatgc agcaggcccg cgtgancctc gccgcgtcgg tcgcgcgtac ntacaantng ctcgcgcagc tgtacgcgct gcgcgacatc gccgagcgcg aaatcaagaa ccgnnnnacg atcggngcga tcacgaacga ncgcgngcgn nnnggcctcg acacgaancg tcgagcgtca gacggcnnan gncnnnntcn cgaccngtcn ntnnaacctg acggagcntg gacggncaga tcnnnngcta cnaactngnn nnnctgctnn nnnnnagggc cnngancgcn gactgnanat cnncatgnnn ggngnncgaa ccccgnnnnn nnnttcnntn ccnannnncg ngnnccnncn nannnnngtc nnnnncnnnn ngacatccnt cnncnnnnnn ngnnannnnc nnanncnncn nnnnnnnnnn tnnaagnaa
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMANYFDLKD DRQIASTSQF EASQSLPAQG GQWPALDWAN QFGDPQLPKL IAEALEGNPS IAQAQARIAK ASSYIETSKS ALYPKVEGSY SWTRELYSAN ALFPPPYGGT WYSENNVLAS ASWDLDLWGK NRERLGQAVS QEKAAEADMQ QARVTLAASV ARTYNSLAQL YALRDIAERE IKNRQTIGAI TNDRVRAGLD TNVERQTANG NIATSQSNLT ELDGQITSTR YELGTLLGKG PDRGLQIAMP VFDPGSAVSL PDNVPADLVS RRPDIVAARW QVEAAMHNVK EAKAEFFPDV NLAAAFGFDA FGWSKFLTSS SRQIQFGPAI HLPIFDAGAL RSQLKGRFAD FDLDVANYNQ TLIGALSDVA TQISSIRSID RQAGDAQRAL DASTKAYELA VIRYKAGLSP QLQVLTADQN RLAAEQTVTN LKMRRRDLQF ALIKALGGGF DATQTGLVVP TDTPPASSAA AAPATHAAN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGAACTAC TTCGATCTCA AGGACGACAG GCAGATCGCG TCGACCTCGC AGTTCGAGGC GTCACAGAGC CTGCCCGCGC AAGGCGGCCA ATGGCCGGCG CTCGACTGGG CGAACCAGTT CGGCGATCCG CAACTGCCGA AGCTGATCGC GGAAGCGCTC GAAGGCAATC CGTCAATCGC GCAGGCGCAG GCGCGCATCG CGAAGGCGTC GTCCTATATC GAAACCTCGA AGTCGGCGCT CTATCCGAAG GTCGAGGGCA GCTATTCGTG GACCCGCGAG CTGTACTCTG CCAATGCGCT GTTTCCGCCG CCGTACGGCG GCACGTGGTA CAGCGAGAAC AATGTGCTCG CCAGCGCGTC GTGGGATCTC GACTTGTGGG GTAAGAACCG GGAACGGCTC GGCCAGGCTG TGTCGCAGGA AAAAGCTGCC GAAGCCGACA TGCAGCAGGC CCGCGTGACC CTCGCCGCGT CGGTCGCGCG TACCTACAAC TCGCTCGCGC AGCTGTACGC GCTGCGCGAC ATCGCCGAGC GCGAAATCAA GAACCGCCAG ACGATCGGCG CGATCACGAA CGACCGCGTG CGGGCCGGCC TCGACACGAA CGTCGAGCGT CAGACGGCCA ATGGCAACAT CGCGACCAGT CAGTCGAACC TGACGGAGCT GGACGGCCAG ATCACGAGCA CGCGCTACGA ACTCGGCACG CTGCTCGGCA AAGGGCCTGA CCGCGGCCTG CAGATCGCCA TGCCGGTGTT CGATCCCGGC AGCGCCGTTT CGCTGCCCGA CAACGTGCCC GCCGACCTCG TGTCGCGCCG GCCGGACATC GTCGCCGCGC GCTGGCAGGT CGAAGCCGCG ATGCACAACG TGAAGGAAGC CAAGGCCGAG TTTTTTCCTG ACGTGAATCT CGCGGCCGCG TTCGGCTTCG ACGCGTTCGG CTGGAGCAAA TTTCTGACGT CGTCGAGCCG TCAGATCCAG TTCGGGCCGG CCATCCATCT GCCGATTTTC GATGCCGGCG CGCTGCGCTC GCAGCTCAAG GGCCGCTTCG CCGATTTCGA CCTCGACGTC GCGAACTACA ACCAGACGCT GATCGGCGCG CTCTCCGACG TCGCCACGCA GATTTCGTCG ATCCGTTCCA TCGACAGGCA GGCGGGCGAC GCACAGCGCG CGCTCGATGC ATCGACGAAA GCGTACGAAC TCGCCGTGAT CCGCTACAAG GCCGGTTTGT CGCCGCAGTT GCAGGTACTG ACGGCCGACC AGAACCGCCT CGCTGCCGAG CAGACGGTGA CGAACCTCAA GATGCGCCGG CGCGATCTGC AATTCGCGCT GATCAAGGCG CTCGGCGGCG GTTTCGACGC GACGCAGACG GGCCTCGTCG TGCCGACGGA TACGCCTCCT GCATCGTCGG CAGCTGCGGC GCCGGCAACG CACGCGGCGA ACTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA