BuphA.01673.c

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID: BuphA.01673.c
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01673.c.A2.GE32194 Mature protein( BuphA.01673.c ) 24 497
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1345
RefSeq: YP_001857496.1
UniProt: B2JHV8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLFSMTRTS CAVAAASLFA TACAFGPSGN PPAMPEPAHY GAEEQSTQTV PAQGITQQFV VGANAVPQWW RTFESNELNA LVDEGLRNSP TLAAADRNLT AAREQLRAQI GSNMLPAIDA GGQAARQRAL GIPEAGPNTF LYNTFVGQLQ AQYTFDIFGA ARLANAALAS RVNVQAFQFN AARRALAANI VTAAVSAAML DAQVKTTERL VTLADEQARD TQRRYDLGAV SHSDLLNAQQ SAASLAASLP AARQQLLTTR HALAVLLGST PDAAPPPLDL ASLHVPERVP VSVPSDLLKA RPDIEAADAT LKAAAADVGV ATAQMYPSIT LNASMGQGGF SWPVALSGAG ALWSIGASIT QPIFHGGALF AQRRAALASY EAATSQYKQT VLTAFQNVAD TLAALEHDAQ SLDAANVAAR AAQRAFEETS AKYRLGAVPV TSTRSSEQQY LNARLDEIRY TGARLNDTAA LFQAMGNPPA EEARAESVST GAGPIAQ
NT Sequence
ATGCGCCTCT TTTCAATGAC CCGCACGTCT TGCGCAGTGG CGGCAGCTTC CCTGTTCGCG ACGGCCTGCG CGTTCGGGCC GAGCGGCAAT CCGCCAGCGA TGCCCGAACC CGCGCATTAC GGCGCCGAAG AACAGTCAAC GCAGACAGTG CCCGCGCAAG GCATCACGCA GCAGTTCGTC GTCGGCGCAA ACGCGGTGCC GCAATGGTGG CGCACGTTTG AATCGAATGA ACTGAACGCG CTCGTCGACG AAGGCCTGCG CAACAGCCCC ACGCTCGCCG CCGCCGACAG GAATCTGACG GCAGCCCGCG AGCAGTTGCG GGCGCAGATC GGCAGCAACA TGCTGCCCGC CATCGACGCC GGCGGCCAGG CTGCGCGCCA GCGCGCGCTC GGCATTCCCG AGGCCGGACC GAACACGTTT CTGTACAACA CGTTCGTCGG CCAGTTGCAG GCGCAGTACA CGTTCGACAT CTTCGGCGCG GCGCGGCTTG CGAACGCGGC GCTGGCCTCG CGTGTGAACG TGCAGGCGTT CCAGTTCAAC GCGGCGCGCC GTGCGCTTGC TGCAAACATT GTGACGGCGG CCGTTTCCGC GGCGATGCTC GATGCCCAGG TGAAGACCAC CGAACGCCTC GTCACGCTCG CCGACGAGCA GGCTCGCGAC ACGCAGCGCC GCTACGATCT CGGCGCGGTA TCGCACAGCG ACCTGCTCAA TGCGCAGCAA AGCGCGGCGT CGTTGGCGGC GAGTCTGCCC GCTGCGCGCC AGCAGCTGCT GACGACGCGT CACGCGCTTG CCGTGCTGCT GGGCAGCACG CCGGACGCCG CGCCGCCGCC GCTCGATCTT GCATCGTTGC ATGTGCCCGA ACGGGTGCCC GTGTCGGTGC CGTCCGATTT ACTGAAAGCG CGCCCGGACA TCGAGGCCGC CGACGCGACG CTGAAGGCAG CCGCGGCCGA TGTCGGCGTC GCGACGGCGC AGATGTATCC GAGCATCACG TTGAACGCGT CGATGGGCCA GGGCGGCTTC AGCTGGCCCG TCGCGCTCTC GGGCGCGGGC GCGCTCTGGA GCATCGGCGC ATCGATCACG CAGCCGATCT TTCACGGCGG CGCATTGTTT GCGCAGCGGC GCGCGGCGCT CGCTTCGTAT GAAGCCGCCA CGTCGCAGTA CAAGCAAACG GTGCTGACTG CCTTTCAGAA CGTCGCCGAT ACATTGGCTG CGTTGGAACA TGACGCGCAA TCGCTCGATG CGGCGAATGT GGCCGCGCGC GCGGCGCAAC GCGCGTTCGA AGAGACGTCC GCGAAGTACC GATTGGGCGC CGTCCCCGTC ACGTCGACGC GCTCCAGCGA GCAGCAATAC CTGAACGCGC GGCTCGACGA GATTCGCTAT ACGGGCGCGC GTCTGAACGA TACGGCGGCG CTGTTCCAGG CGATGGGCAA TCCGCCTGCC GAGGAGGCGC GCGCTGAGTC GGTGTCGACG GGTGCGGGAC CCATTGCACA G
Details for BuphA.01673.c.A2.GE32194
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MAFGPSGNPP AMPEPAHYGA EEQSTQTVPA QGITQXFVVG ANAVPQWWRT FESNELNALV DEGLRNSPTL AAADRNLTAA REQLRAQIGS NMLPAIDAGG QAARQRALGI PEAGPNTFLY NTFVGQLQAQ YTFDIFGAAR LANAALASRV NVQAFQFNAA RRALAAXIVT AAVSAAMLDA QVKTTERLVT LADEQARDTQ RRYDLGAVSH SDLLNAQQSA ASXAASLPXA RQQLLTTRHA LAVLLGSTPD AAPPPLDLAS LHVPERVPVS VPSDLLKARP DIEAADATLK AAAADVGVAT AQMYPSITLN ASMGQGGFSW PVALSGAGAL WSIGASITQP IFHGGALFAQ RRAALASYEA ATSQYKQTVL TAFQNVADTL AALEHDAQSL DAANVAARAA QRAFEETSAK YRLGAVPVTS TRSSEQQYLN ARLDEIRYTG ARLNDTAALF QAMGNPPAEE ARAESVSTGA GPIAQ
Validated NT Sequence
actgtgcaat gggtcccgca cccgtcgaca ccgactcagc gcgcgcctcc tcggcaggcg gattgcccat cgcctggaac agcgccgccg tatcgttcag acgcgcgccc gtatagcgaa tctcgtcgag ccgcgcgttc aggtattgct gctcgctgga gcgcgtcgac gtgacgggga cggcgcccaa tcggtacttc gcggacgtct cttcgaacgc gcgttgcgcc gcgcgcgcgg ccacattcgc cgcatcgagc gattgcgcgt catgttccaa cgcagccaat gtatcggcga cgttctgaaa ggcagtcagc accgtttgct tgtactgcga cgtggcggct tcatacgaag cgagcgccgc gcgccgctgc gcaaacaatg cgccgccgtg aaagatcggc tgcgtgatcg atgcgccgat gctccagagc gcgcccgcgc ccgagagcgc gacgggccag ctgaagccgc cctggcccat cgacgcgttc aacgtgatgc tcggatacat ctgcgccgtc gcgacgccga catcggccgc ggctgccttc agcgtcgcgt cggcggcctc gatgtccggg cgcgctttca gtaaatcgga cggcaccgac acgggcaccc gttcgggcac atgcaacgat gcaagatcga gcggcggcgg cgcggcgtcc ggcgtgctgc ccagcagcac ggcaagcgcg tgacgcgtcg tcagcagctg ctggcgcgca ncgggcagac tcgccgccna cgacgccgcg ctttgctgcg cattgagcag gtcgctgtgc gataccgcgc cgagatcgta gcggcgctgc gtgtcgcgag cctgctcgtc ggcgagcgtg acgaggcgtt cggtggtctt cacctgggca tcgagcatcg ccgcggaaac ggccgccgtc acaatgtntg cagcaagcgc acggcgcgcc gcgttgaact ggaacgcctg cacgttcaca cgcgaggcca gcgccgcgtt cgcaagccgc gccgcgccga agatgtcgaa cgtgtactgc gcctgcaact ggccgacgaa cgtgttgtac agaaacgtgt tcggtccggc ctcgggaatg ccgagcgcgc gctggcgcgc agcctggccg ccggcgtcga tggcgggcag catgttgctg ccgatctgcg cccgcaactg ctcgcgggct gccgtcagat tcctgtcggc ggcggcgagc gtggggctgt tgcgcaggcc ttcgtcgacg agcgcgttca gttcattcga ttcaaacgtg cgccaccatt gcggcaccgc gtttgcgccg acgacgaant gctgcgtgat gccttgcgcg ggcactgtct gcgttgactg ttcttcggcg ccgtaatgcg cgggttcggg catcgctggc ggattgccgc tcggcccgaa cgccatcgaa ccaggacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAFGPSGNP PAMPEPAHYG AEEQSTQTVP AQGITQQFVV GANAVPQWWR TFESNELNAL VDEGLRNSPT LAAADRNLTA AREQLRAQIG SNMLPAIDAG GQAARQRALG IPEAGPNTFL YNTFVGQLQA QYTFDIFGAA RLANAALASR VNVQAFQFNA ARRALAANIV TAAVSAAMLD AQVKTTERLV TLADEQARDT QRRYDLGAVS HSDLLNAQQS AASLAASLPA ARQQLLTTRH ALAVLLGSTP DAAPPPLDLA SLHVPERVPV SVPSDLLKAR PDIEAADATL KAAAADVGVA TAQMYPSITL NASMGQGGFS WPVALSGAGA LWSIGASITQ PIFHGGALFA QRRAALASYE AATSQYKQTV LTAFQNVADT LAALEHDAQS LDAANVAARA AQRAFEETSA KYRLGAVPVT STRSSEQQYL NARLDEIRYT GARLNDTAAL FQAMGNPPAE EARAESVSTG AGPIAQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgttcggg ccgagcggca atccgccagc gatgcccgaa cccgcgcatt acggcgccga agaacagtca acgcagacag tgcccgcgca aggcatcacg cagcagttcg tcgtcggcgc aaacgcggtg ccgcaatggt ggcgcacgtt tgaatcgaat gaactgaacg cgctcgtcga cgaaggcctg cgcaacagcc ccacgctcgc cgccgccgac aggaatctga cggcagcccg cgagcagttg cgggcgcaga tcggcagcaa catgctgccc gccatcgacg ccggcggcca ggctgcgcgc cagcgcgcgc tcggcattcc cgaggccgga ccgaacacgt ttctgtacaa cacgttcgtc ggccagttgc aggcgcagta cacgttcgac atcttcggcg cggcgcggct tgcgaacgcg gcgctggcct cgcgtgtgaa cgtgcaggcg ttccagttca acgcggcgcg ccgtgcgctt gctgcaaaca ttgtgacggc ggccgtttcc gcggcgatgc tcgatgccca ggtgaagacc accgaacgcc tcgtcacgct cgccgacgag caggctcgcg acacgcagcg ccgctacgat ctcggcgcgg tatcgcacag cgacctgctc aatgcgcagc aaagcgcggc gtcgttggcg gcgagtctgc ccgctgcgcg ccagcagctg ctgacgacgc gtcacgcgct tgccgtgctg ctgggcagca cgccggacgc cgcgccgccg ccgctcgatc ttgcatcgtt gcatgtgccc gaacgggtgc ccgtgtcggt gccgtccgat ttactgaaag cgcgcccgga catcgaggcc gccgacgcga cgctgaaggc agccgcggcc gatgtcggcg tcgcgacggc gcagatgtat ccgagcatca cgttgaacgc gtcgatgggc cagggcggct tcagctggcc cgtcgcgctc tcgggcgcgg gcgcgctctg gagcatcggc gcatcgatca cgcagccgat ctttcacggc ggcgcattgt ttgcgcagcg gcgcgcggcg ctcgcttcgt atgaagccgc cacgtcgcag tacaagcaaa cggtgctgac tgcctttcag aacgtcgccg atacattggc tgcgttggaa catgacgcgc aatcgctcga tgcggcgaat gtggccgcgc gcgcggcgca acgcgcgttc gaagagacgt ccgcgaagta ccgattgggc gccgtccccg tcacgtcgac gcgctccagc gagcagcaat acctgaacgc gcggctcgac gagattcgct atacgggcgc gcgtctgaac gatacggcgg cgctgttcca ggcgatgggc aatccgcctg ccgaggaggc gcgcgctgag tcggtgtcga cgggtgcggg acccattgca cagtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa