SSGCID: Target Page

BuphA.01673.a

RND efflux system, outer membrane lipoprotein, NodT family

CENTER ID:	BuphA.01673.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.01673.a.A2.GE34743	Mature protein( BuphA.01673.a )		20	505

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.01673.a.A2.PW35353	Mature protein( BuphA.01673.a )		20	505

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.503
RefSeq:	YP_001856718.1
UniProt:	B2JDF5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHKYSLMAVA VALFATGCTM EPTYHQPAAP VSGTFPSDGV YATQPVPGAG ARSANGQPAV EIGWRDFFVD QRLQRLIEIA LKNNRDLRVS VLNIEASRAQ YQIARAALFP ALDASASQTK QRTPKNLSVF GNTISNEYSV GLNASWEIDF FGRIRSLKDQ ALAQYLATAQ ARKAAEIALV ASVADQYMTV LAYDDLLKVT ENTLKSAQES YRITKLQYDT GTGSELDLRQ AQTVVEQATA NLQSQARLRA QAENALVLLV GEPLPSDMPS GLILDDQNLL TDIPAGLPSD LLTRRPDIME AEQNLLAANA NIGAARAAFF PKVSLTGSFG TLSPTLGGLF KPGSAAWSFA PTITLPIFEG GQNIANLDLA NVEKNIQIAQ YEKAIQTAFR EVADGLAARG TYDEQIKALE RDTFAEQRRL DLSDLRYRNG VDSYLAVLTA QTSLYSAQQL LITARMERLT NLVDLYKALG GGWIEHAGDQ PRPADAPVDY GAASAPVAAS AATAG

NT Sequence

ATGCATAAAT ATTCGTTGAT GGCAGTGGCC GTCGCGCTGT TCGCCACGGG CTGCACGATG GAGCCGACGT ACCACCAGCC GGCCGCGCCC GTTTCGGGCA CGTTCCCGAG CGACGGCGTG TACGCGACGC AACCCGTGCC CGGTGCGGGC GCGCGCTCGG CGAACGGCCA GCCGGCGGTG GAGATCGGCT GGCGCGACTT CTTCGTCGAT CAGCGCCTGC AACGTCTGAT CGAGATCGCG CTGAAGAACA ATCGCGATCT GCGCGTGTCG GTGCTGAACA TCGAGGCTTC GCGCGCGCAA TATCAGATTG CGCGCGCGGC GCTGTTCCCG GCGCTCGACG CTTCCGCGTC GCAGACGAAG CAGCGCACGC CGAAGAATCT GTCGGTGTTC GGCAATACGA TTTCCAACGA GTATTCGGTC GGCTTGAATG CTTCGTGGGA AATCGACTTC TTCGGCCGGA TTCGCAGCCT GAAGGACCAG GCACTCGCGC AGTATCTGGC GACGGCACAG GCACGCAAGG CGGCGGAAAT CGCGCTGGTC GCATCGGTCG CGGATCAGTA CATGACCGTG CTGGCCTATG ACGATCTGCT GAAGGTCACG GAGAACACGC TTAAGAGCGC GCAAGAGTCT TATCGGATCA CGAAGTTGCA GTACGACACG GGTACGGGTT CTGAACTCGA CCTGCGTCAG GCTCAGACCG TTGTCGAGCA GGCGACTGCG AACCTGCAGT CGCAGGCGCG TTTGCGGGCG CAGGCTGAGA ACGCACTTGT TCTCCTTGTT GGTGAGCCGT TGCCGTCTGA TATGCCGAGC GGACTTATCC TCGACGATCA GAATCTGCTG ACGGACATTC CGGCCGGTCT GCCGTCGGAT CTGCTCACGC GACGTCCCGA CATCATGGAG GCCGAGCAGA ACCTGCTGGC GGCCAACGCG AATATCGGTG CGGCGCGCGC GGCCTTCTTC CCGAAGGTTT CGTTGACGGG CAGCTTCGGC ACGCTGAGCC CGACACTCGG CGGGCTGTTC AAGCCAGGTT CGGCGGCGTG GAGCTTCGCG CCGACGATCA CGCTGCCGAT CTTCGAAGGC GGCCAGAACA TCGCGAACCT GGATCTCGCC AACGTCGAGA AGAACATCCA GATCGCGCAG TACGAAAAGG CGATTCAGAC GGCGTTTCGC GAGGTGGCCG ACGGGCTCGC GGCGCGCGGC ACCTACGATG AGCAGATCAA GGCGCTCGAA CGCGATACGT TCGCGGAACA GCGTCGACTG GATCTGTCGG ACCTGCGTTA CCGGAATGGC GTCGACAGCT ATCTCGCGGT GCTGACCGCG CAGACCAGCC TGTATAGCGC ACAACAGCTG CTGATCACGG CGCGCATGGA GCGTCTGACG AACCTCGTCG ATCTGTACAA GGCGCTCGGC GGCGGCTGGA TCGAACATGC AGGCGATCAG CCGCGTCCGG CGGACGCGCC TGTCGATTAC GGCGCGGCCA GCGCGCCTGT TGCCGCTTCG GCCGCGACGG CGGGC

Details for BuphA.01673.a.A2.GE34743

HARVESTED ON:	3/13/2012
SEQUENCED ON:	3/13/2012
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Validated AA Sequence

MEPTYHQPAA PVSGTFPSDG VYATQPVPGA GARSANGQPA VEIGWRDFFV DQRLQRLIEI ALKNNRDLRV SVLNIEASRA QYQIARAALF PALDASASQT KQRTPKNLSV FGNTISNEYS VGLNASWEID FFGRIRSLKD QALAQYLATA QARKAAEIAL VASVADQYMT VLAYDDLLKV TENTLKSAQE SYRITKLQYD TGTGSELDLR QAQTVVEQAT ANLQSQARLR AQAENALVLL VGEPLPSDMP SGLILDDQNL LTDIPAGLPS DLLTRRPDIM EAEQNLLAAN ANIGAARAAF FPKVSLTGSF GTLSPTLGGL FKPGSAAWSF APTITLPIFE GGQNIANLDL ANVEKNIQIA QYEKAIQTAF REVADGLAAR GTYDEQIKAL ERDTFAEQRR LDLSDLRYRN GVDSYLAVLT AQTSLYSAQQ LLITARMERL TNLVDLYKAL GGGWIEHAGD QPRPADAPVD YGAASAPVAA SAATAG

Validated NT Sequence

agcccgccgt cgcggccgaa gcggcaacag gcgcgctggc cgcgccgtaa tcgacaggcg cgtccgccgg acgcggctga tcgcctgcat gttcgatcca gccgccgccg agcgccttgt acagatcgac gaggttcgtc agacgctcca tgcgcgccgt gatcagcagc tgttgtgcgc tatacaggct ggtctgcgcg gtcagcaccg cgagatagct gtcgacgcca ttccggtaac gcaggtccga cagatccagt cgacgctgtt ccgcgaacgt atcgcgttcg agcgccttga tctgctcatc gtaggtgccg cgcgccgcga gcccgtcggc cacctcgcga aacgccgtct gaatcgcctt ttcgtactgc gcgatctgga tgttcttctc gacgttggcg agatccaggt tcgcgatgtt ctggccgcct tcgaagatcg gcagcgtgat cgtcggcgcg aagctccacg ccgccgaacc tggcttgaac agcccgccga gtgtcgggct cagcgtgccg aagctgcccg tcaacgaaac cttcgggaag aaggccgcgc gcgccgcacc gatattcgcg ttggccgcca gcaggttctg ctcggcctcc atgatgtcgg gacgtcgcgt gagcagatcc gacggcagac cggccggaat gtccgtcagc agattctgat cgtcgaggat aagtccgctc ggcatatcag acggcaacgg ctcaccaaca aggagaacaa gtgcgttctc agcctgcgcc cgcaaacgcg cctgcgactg caggttcgca gtcgcctgct cgacaacggt ctgagcctga cgcaggtcga gttcagaacc cgtacccgtg tcgtactgca acttcgtgat ccgataagac tcttgcgcgc tcttaagcgt gttctccgtg accttcagca gatcgtcata ggccagcacg gtcatgtact gatccgcgac cgatgcgacc agcgcgattt ccgccgcctt gcgtgcctgt gccgtcgcca gatactgcgc gagtgcctgg tccttcaggc tgcgaatccg gccgaagaag tcgatttccc acgaagcatt caagccgacc gaatactcgt tggaaatcgt attgccgaac accgacagat tcttcggcgt gcgctgcttc gtctgcgacg cggaagcgtc gagcgccggg aacagcgccg cgcgcgcaat ctgatattgc gcgcgcgaag cctcgatgtt cagcaccgac acgcgcagat cgcgattgtt cttcagcgcg atctcgatca gacgttgcag gcgctgatcg acgaagaagt cgcgccagcc gatctccacc gccggctggc cgttcgccga gcgcgcgccc gcaccgggca cgggttgcgt cgcgtacacg ccgtcgctcg ggaacgtgcc cgaaacgggc gcggccggct ggtggtacgt cggctccatc gaaccaggac cctgggtctg agcttccag

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMEPTYHQPA APVSGTFPSD GVYATQPVPG AGARSANGQP AVEIGWRDFF VDQRLQRLIE IALKNNRDLR VSVLNIEASR AQYQIARAAL FPALDASASQ TKQRTPKNLS VFGNTISNEY SVGLNASWEI DFFGRIRSLK DQALAQYLAT AQARKAAEIA LVASVADQYM TVLAYDDLLK VTENTLKSAQ ESYRITKLQY DTGTGSELDL RQAQTVVEQA TANLQSQARL RAQAENALVL LVGEPLPSDM PSGLILDDQN LLTDIPAGLP SDLLTRRPDI MEAEQNLLAA NANIGAARAA FFPKVSLTGS FGTLSPTLGG LFKPGSAAWS FAPTITLPIF EGGQNIANLD LANVEKNIQI AQYEKAIQTA FREVADGLAA RGTYDEQIKA LERDTFAEQR RLDLSDLRYR NGVDSYLAVL TAQTSLYSAQ QLLITARMER LTNLVDLYKA LGGGWIEHAG DQPRPADAPV DYGAASAPVA ASAATAG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggagccgacg taccaccagc cggccgcgcc cgtttcgggc acgttcccga gcgacggcgt gtacgcgacg caacccgtgc ccggtgcggg cgcgcgctcg gcgaacggcc agccggcggt ggagatcggc tggcgcgact tcttcgtcga tcagcgcctg caacgtctga tcgagatcgc gctgaagaac aatcgcgatc tgcgcgtgtc ggtgctgaac atcgaggctt cgcgcgcgca atatcagatt gcgcgcgcgg cgctgttccc ggcgctcgac gcttccgcgt cgcagacgaa gcagcgcacg ccgaagaatc tgtcggtgtt cggcaatacg atttccaacg agtattcggt cggcttgaat gcttcgtggg aaatcgactt cttcggccgg attcgcagcc tgaaggacca ggcactcgcg cagtatctgg cgacggcaca ggcacgcaag gcggcggaaa tcgcgctggt cgcatcggtc gcggatcagt acatgaccgt gctggcctat gacgatctgc tgaaggtcac ggagaacacg cttaagagcg cgcaagagtc ttatcggatc acgaagttgc agtacgacac gggtacgggt tctgaactcg acctgcgtca ggctcagacc gttgtcgagc aggcgactgc gaacctgcag tcgcaggcgc gtttgcgggc gcaggctgag aacgcacttg ttctccttgt tggtgagccg ttgccgtctg atatgccgag cggacttatc ctcgacgatc agaatctgct gacggacatt ccggccggtc tgccgtcgga tctgctcacg cgacgtcccg acatcatgga ggccgagcag aacctgctgg cggccaacgc gaatatcggt gcggcgcgcg cggccttctt cccgaaggtt tcgttgacgg gcagcttcgg cacgctgagc ccgacactcg gcgggctgtt caagccaggt tcggcggcgt ggagcttcgc gccgacgatc acgctgccga tcttcgaagg cggccagaac atcgcgaacc tggatctcgc caacgtcgag aagaacatcc agatcgcgca gtacgaaaag gcgattcaga cggcgtttcg cgaggtggcc gacgggctcg cggcgcgcgg cacctacgat gagcagatca aggcgctcga acgcgatacg ttcgcggaac agcgtcgact ggatctgtcg gacctgcgtt accggaatgg cgtcgacagc tatctcgcgg tgctgaccgc gcagaccagc ctgtatagcg cacaacagct gctgatcacg gcgcgcatgg agcgtctgac gaacctcgtc gatctgtaca aggcgctcgg cggcggctgg atcgaacatg caggcgatca gccgcgtccg gcggacgcgc ctgtcgatta cggcgcggcc agcgcgcctg ttgccgcttc ggccgcgacg gcgggctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa

Details for BuphA.01673.a.A2.PW35353

PURIFICATION DATe:	4/13/2012
CONCENTRATION:	24.24mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	20
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGAGCCGACG TACCACCAGC CGGCCGCGCC CGTTTCGGGC ACGTTCCCGA GCGACGGCGT GTACGCGACG CAACCCGTGC CCGGTGCGGG CGCGCGCTCG GCGAACGGCC AGCCGGCGGT GGAGATCGGC TGGCGCGACT TCTTCGTCGA TCAGCGCCTG CAACGTCTGA TCGAGATCGC GCTGAAGAAC AATCGCGATC TGCGCGTGTC GGTGCTGAAC ATCGAGGCTT CGCGCGCGCA ATATCAGATT GCGCGCGCGG CGCTGTTCCC GGCGCTCGAC GCTTCCGCGT CGCAGACGAA GCAGCGCACG CCGAAGAATC TGTCGGTGTT CGGCAATACG ATTTCCAACG AGTATTCGGT CGGCTTGAAT GCTTCGTGGG AAATCGACTT CTTCGGCCGG ATTCGCAGCC TGAAGGACCA GGCACTCGCG CAGTATCTGG CGACGGCACA GGCACGCAAG GCGGCGGAAA TCGCGCTGGT CGCATCGGTC GCGGATCAGT ACATGACCGT GCTGGCCTAT GACGATCTGC TGAAGGTCAC GGAGAACACG CTTAAGAGCG CGCAAGAGTC TTATCGGATC ACGAAGTTGC AGTACGACAC GGGTACGGGT TCTGAACTCG ACCTGCGTCA GGCTCAGACC GTTGTCGAGC AGGCGACTGC GAACCTGCAG TCGCAGGCGC GTTTGCGGGC GCAGGCTGAG AACGCACTTG TTCTCCTTGT TGGTGAGCCG TTGCCGTCTG ATATGCCGAG CGGACTTATC CTCGACGATC AGAATCTGCT GACGGACATT CCGGCCGGTC TGCCGTCGGA TCTGCTCACG CGACGTCCCG ACATCATGGA GGCCGAGCAG AACCTGCTGG CGGCCAACGC GAATATCGGT GCGGCGCGCG CGGCCTTCTT CCCGAAGGTT TCGTTGACGG GCAGCTTCGG CACGCTGAGC CCGACACTCG GCGGGCTGTT CAAGCCAGGT TCGGCGGCGT GGAGCTTCGC GCCGACGATC ACGCTGCCGA TCTTCGAAGG CGGCCAGAAC ATCGCGAACC TGGATCTCGC CAACGTCGAG AAGAACATCC AGATCGCGCA GTACGAAAAG GCGATTCAGA CGGCGTTTCG CGAGGTGGCC GACGGGCTCG CGGCGCGCGG CACCTACGAT GAGCAGATCA AGGCGCTCGA ACGCGATACG TTCGCGGAAC AGCGTCGACT GGATCTGTCG GACCTGCGTT ACCGGAATGG CGTCGACAGC TATCTCGCGG TGCTGACCGC GCAGACCAGC CTGTATAGCG CACAACAGCT GCTGATCACG GCGCGCATGG AGCGTCTGAC GAACCTCGTC GATCTGTACA AGGCGCTCGG CGGCGGCTGG ATCGAACATG CAGGCGATCA GCCGCGTCCG GCGGACGCGC CTGTCGATTA CGGCGCGGCC AGCGCGCCTG TTGCCGCTTC GGCCGCGACG GCGGGCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA