BuphA.01669.a

Response regulator receiver sensor signal transduction histidine kinase

CENTER ID: BuphA.01669.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01669.a.A1.GE32182 Full length( BuphA.01669.a ) 1 386
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01669.a.A1.PS01135 Full length( BuphA.01669.a ) 1 386

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4787
RefSeq: YP_001860688.1
UniProt: B2JQV1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MREHNKDAPV VLVVDDTAAN LGLVVDTLEA EGLRVAVARD GHEALRRAEL VKPDLILLDV MMPGLDGFQT CRALKDNPVT RDIPVIFMTS LTQTEDKIMG FRVGAMDFVT KPLQMEEVAV RVQTHLKLRA LQRLQQEQNA RLEEEIKTRI QAQDALIEVL NGVRNVSNAI AHDLRTPLTE LRSRLEVLLL GLRKKGDEET LGQLEVAMAD VDRVIGIFNA LLRLAEIDAG VRRSGFVRSD VVTILSDAVE FYQPVAELRG ISLTLLLCSE REVLAEVDPL LLAQAIGNLI DNALKYAQDN GEVEVSLCER KDRIDVTVSD DGPGIPFTER SKVTERFYRG DRSRGTPGVG LGLALVKAVA TLHHGFLEFT DNEPGLAATM TILRVS
NT Sequence
ATGCGAGAGC ACAATAAAGA CGCGCCCGTC GTGCTTGTGG TCGACGACAC GGCGGCGAAT CTGGGGCTCG TCGTCGATAC GCTCGAAGCC GAAGGGCTGC GCGTGGCCGT CGCGCGCGAC GGCCACGAAG CGCTGCGCCG CGCCGAACTG GTCAAGCCCG ACCTGATCCT GCTCGACGTG ATGATGCCAG GTCTCGACGG TTTTCAGACG TGTCGCGCGC TCAAGGACAA TCCCGTCACG CGGGATATTC CCGTGATCTT CATGACCTCG CTAACGCAGA CAGAGGACAA GATCATGGGC TTCCGCGTCG GCGCGATGGA TTTCGTGACC AAGCCGCTGC AAATGGAAGA AGTCGCTGTG CGCGTGCAGA CGCATCTCAA GCTGCGTGCG TTGCAAAGGT TGCAGCAGGA ACAGAATGCC CGGCTGGAAG AGGAGATCAA GACGCGCATT CAGGCGCAGG ATGCGCTGAT CGAAGTGCTG AACGGGGTGC GCAACGTGTC AAACGCCATC GCGCACGATC TCCGGACGCC GCTCACGGAG CTGCGATCGA GACTCGAGGT GCTGCTGCTC GGCTTGCGCA AGAAGGGCGA CGAAGAGACG CTGGGCCAGC TCGAAGTGGC GATGGCCGAC GTGGACCGCG TGATCGGCAT TTTCAATGCG CTGCTGCGTC TCGCCGAGAT CGATGCGGGC GTGCGGCGCT CGGGTTTTGT CAGAAGCGAC GTCGTGACTA TCCTGTCGGA CGCCGTCGAG TTCTATCAGC CCGTCGCGGA GTTACGCGGC ATTTCGCTGA CGCTGTTGCT ATGTTCGGAA AGGGAGGTAC TGGCCGAAGT CGACCCGTTG CTGCTTGCGC AGGCGATCGG TAATCTGATC GACAACGCGC TGAAGTACGC GCAAGACAAC GGCGAGGTCG AGGTGTCGCT GTGTGAGCGT AAGGATCGTA TCGACGTCAC GGTGTCGGAT GACGGACCCG GTATCCCCTT CACCGAACGG TCCAAGGTGA CAGAGCGGTT CTATCGCGGC GACCGGAGCC GGGGAACCCC TGGCGTCGGA CTCGGGCTCG CACTCGTCAA AGCGGTGGCG ACGCTGCACC ACGGTTTTCT GGAGTTTACT GACAACGAGC CAGGCCTTGC TGCCACCATG ACGATCCTCC GGGTCAGC
Details for BuphA.01669.a.A1.GE32182
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MREHNKDAPV VLVVDDTAAN LGLVVDTLEA EGLRVAVARD GHEALRRAEL VKPDLILLDV MMPGLDGFQT CRALKDNPVT RDIPVIFMTS LTQTEDKIMG FRVGAMDFVT KPLQMEEVAV RVQTHLKLRA LQRLQQEQNA RLEEEIKTRI QAQDALIEVL NGVRNVSNAI AHDLRTPLTE LRSRLEVLLL GLRKKGDEET LGQLEVAMAD VDRVIGIFNA LLRLAEIDAG VRRSGFVRSD VVTILSDAVE FYQPVAELRG ISLTLLLCSE REVLAEVDPL LLAQAIGNLI DNALKYAQDN GEVEVSLCER KDRIDVTVSD DGPGIPFTER SKVTERFYRG DRSRGTPGVG LGLALVKAVA TLHHGFLEFT DNEPGLAATM TI
Validated NT Sequence
ggatcgtcat ggtggcagca aggcctggct cgttgtcagt aaactccaga aaaccgtggt gcagcgtcgc caccgctttg acgagtgcga gcccgagtcc gacgccaggg gttccccggc tccggtcgcc gcgatagaac cgctctgtca ccttggaccg ttcggtgaag gggataccgg gtccgtcatc cgacaccgtg acgtcgatac gatccttacg ctcacacagc gacacctcga cctcgccgtt gtcttgcgcg tacttcagcg cgttgtcgat cagattaccg atcgcctgcg caagcagcaa cgggtcgact tcggccagta cctccctttc cgaacatagc aacagcgtca gcgaaatgcc gcgtaactcc gcgacgggct gatagaactc gacggcgtcc gacaggatag tcacgacgtc gcttctgaca aaacccgagc gccgcacgcc cgcatcgatc tcggcgagac gcagcagcgc attgaaaatg ccgatcacgc ggtccacgtc ggccatcgcc acttcgagct ggcccagcgt ctcttcgtcg cccttcttgc gcaagccgag cagcagcacc tcgagtctcg atcgcagctc cgtgagcggc gtccggagat cgtgcgcgat ggcgtttgac acgttgcgca ccccgttcag cacttcgatc agcgcatcct gcgcctgaat gcgcgtcttg atctcctctt ccagccgggc attctgttcc tgctgcaacc tttgcaacgc acgcagcttg agatgcgtct gcacgcgcac agcgacttct tccatttgca gcggcttggt cacgaaatcc atcgcgccga cgcggaagcc catgatcttg tcctctgtct gcgttagcga ggtcatgaag atcacgggaa tatcccgcgt gacgggattg tccttgagcg cgcgacacgt ctgaaaaccg tcgagacctg gcatcatcac gtcgagcagg atcaggtcgg gcttgaccag ttcggcgcgg cgcagcgctt cgtggccgtc gcgcgcgacg gccacgcgca gcccttcggc ttcgagcgta tcgacgacga gccccagatt cgccgccgtg tcgtcgacca caagcacgac gggcgcgtct ttattgtgct ctcgcatcga accaggaccc tgggtctgag cttccagggt a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMREHNKDAP VVLVVDDTAA NLGLVVDTLE AEGLRVAVAR DGHEALRRAE LVKPDLILLD VMMPGLDGFQ TCRALKDNPV TRDIPVIFMT SLTQTEDKIM GFRVGAMDFV TKPLQMEEVA VRVQTHLKLR ALQRLQQEQN ARLEEEIKTR IQAQDALIEV LNGVRNVSNA IAHDLRTPLT ELRSRLEVLL LGLRKKGDEE TLGQLEVAMA DVDRVIGIFN ALLRLAEIDA GVRRSGFVRS DVVTILSDAV EFYQPVAELR GISLTLLLCS EREVLAEVDP LLLAQAIGNL IDNALKYAQD NGEVEVSLCE RKDRIDVTVS DDGPGIPFTE RSKVTERFYR GDRSRGTPGV GLGLALVKAV ATLHHGFLEF TDNEPGLAAT MTILRVS
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgagagcac aataaagacg cgcccgtcgt gcttgtggtc gacgacacgg cggcgaatct ggggctcgtc gtcgatacgc tcgaagccga agggctgcgc gtggccgtcg cgcgcgacgg ccacgaagcg ctgcgccgcg ccgaactggt caagcccgac ctgatcctgc tcgacgtgat gatgccaggt ctcgacggtt ttcagacgtg tcgcgcgctc aaggacaatc ccgtcacgcg ggatattccc gtgatcttca tgacctcgct aacgcagaca gaggacaaga tcatgggctt ccgcgtcggc gcgatggatt tcgtgaccaa gccgctgcaa atggaagaag tcgctgtgcg cgtgcagacg catctcaagc tgcgtgcgtt gcaaaggttg cagcaggaac agaatgcccg gctggaagag gagatcaaga cgcgcattca ggcgcaggat gcgctgatcg aagtgctgaa cggggtgcgc aacgtgtcaa acgccatcgc gcacgatctc cggacgccgc tcacggagct gcgatcgaga ctcgaggtgc tgctgctcgg cttgcgcaag aagggcgacg aagagacgct gggccagctc gaagtggcga tggccgacgt ggaccgcgtg atcggcattt tcaatgcgct gctgcgtctc gccgagatcg atgcgggcgt gcggcgctcg ggttttgtca gaagcgacgt cgtgactatc ctgtcggacg ccgtcgagtt ctatcagccc gtcgcggagt tacgcggcat ttcgctgacg ctgttgctat gttcggaaag ggaggtactg gccgaagtcg acccgttgct gcttgcgcag gcgatcggta atctgatcga caacgcgctg aagtacgcgc aagacaacgg cgaggtcgag gtgtcgctgt gtgagcgtaa ggatcgtatc gacgtcacgg tgtcggatga cggacccggt atccccttca ccgaacggtc caaggtgaca gagcggttct atcgcggcga ccggagccgg ggaacccctg gcgtcggact cgggctcgca ctcgtcaaag cggtggcgac gctgcaccac ggttttctgg agtttactga caacgagcca ggccttgctg ccaccatgac gatcctccgg gtcagctaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for BuphA.01669.a.A1.PS01135
PURIFICATION DATe: 6/20/2011
CONCENTRATION: 41.1mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MREHNKDAPV VLVVDDTAAN LGLVVDTLEA EGLRVAVARD GHEALRRAEL VKPDLILLDV MMPGLDGFQT CRALKDNPVT RDIPVIFMTS LTQTEDKIMG FRVGAMDFVT KPLQMEEVAV RVQTHLKLRA LQRLQQEQNA RLEEEIKTRI QAQDALIEVL NGVRNVSNAI AHDLRTPLTE LRSRLEVLLL GLRKKGDEET LGQLEVAMAD VDRVIGIFNA LLRLAEIDAG VRRSGFVRSD VVTILSDAVE FYQPVAELRG ISLTLLLCSE REVLAEVDPL LLAQAIGNLI DNALKYAQDN GEVEVSLCER KDRIDVTVSD DGPGIPFTER SKVTERFYRG DRSRGTPGVG LGLALVKAVA TLHHGFLEFT DNEPGLAATM TI
Validated NT Sequence
ggatcgtcat ggtggcagca aggcctggct cgttgtcagt aaactccaga aaaccgtggt gcagcgtcgc caccgctttg acgagtgcga gcccgagtcc gacgccaggg gttccccggc tccggtcgcc gcgatagaac cgctctgtca ccttggaccg ttcggtgaag gggataccgg gtccgtcatc cgacaccgtg acgtcgatac gatccttacg ctcacacagc gacacctcga cctcgccgtt gtcttgcgcg tacttcagcg cgttgtcgat cagattaccg atcgcctgcg caagcagcaa cgggtcgact tcggccagta cctccctttc cgaacatagc aacagcgtca gcgaaatgcc gcgtaactcc gcgacgggct gatagaactc gacggcgtcc gacaggatag tcacgacgtc gcttctgaca aaacccgagc gccgcacgcc cgcatcgatc tcggcgagac gcagcagcgc attgaaaatg ccgatcacgc ggtccacgtc ggccatcgcc acttcgagct ggcccagcgt ctcttcgtcg cccttcttgc gcaagccgag cagcagcacc tcgagtctcg atcgcagctc cgtgagcggc gtccggagat cgtgcgcgat ggcgtttgac acgttgcgca ccccgttcag cacttcgatc agcgcatcct gcgcctgaat gcgcgtcttg atctcctctt ccagccgggc attctgttcc tgctgcaacc tttgcaacgc acgcagcttg agatgcgtct gcacgcgcac agcgacttct tccatttgca gcggcttggt cacgaaatcc atcgcgccga cgcggaagcc catgatcttg tcctctgtct gcgttagcga ggtcatgaag atcacgggaa tatcccgcgt gacgggattg tccttgagcg cgcgacacgt ctgaaaaccg tcgagacctg gcatcatcac gtcgagcagg atcaggtcgg gcttgaccag ttcggcgcgg cgcagcgctt cgtggccgtc gcgcgcgacg gccacgcgca gcccttcggc ttcgagcgta tcgacgacga gccccagatt cgccgccgtg tcgtcgacca caagcacgac gggcgcgtct ttattgtgct ctcgcatcga accaggaccc tgggtctgag cttccagggt a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMREHNKDAP VVLVVDDTAA NLGLVVDTLE AEGLRVAVAR DGHEALRRAE LVKPDLILLD VMMPGLDGFQ TCRALKDNPV TRDIPVIFMT SLTQTEDKIM GFRVGAMDFV TKPLQMEEVA VRVQTHLKLR ALQRLQQEQN ARLEEEIKTR IQAQDALIEV LNGVRNVSNA IAHDLRTPLT ELRSRLEVLL LGLRKKGDEE TLGQLEVAMA DVDRVIGIFN ALLRLAEIDA GVRRSGFVRS DVVTILSDAV EFYQPVAELR GISLTLLLCS EREVLAEVDP LLLAQAIGNL IDNALKYAQD NGEVEVSLCE RKDRIDVTVS DDGPGIPFTE RSKVTERFYR GDRSRGTPGV GLGLALVKAV ATLHHGFLEF TDNEPGLAAT MTILRVS
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGAGAGCAC AATAAAGACG CGCCCGTCGT GCTTGTGGTC GACGACACGG CGGCGAATCT GGGGCTCGTC GTCGATACGC TCGAAGCCGA AGGGCTGCGC GTGGCCGTCG CGCGCGACGG CCACGAAGCG CTGCGCCGCG CCGAACTGGT CAAGCCCGAC CTGATCCTGC TCGACGTGAT GATGCCAGGT CTCGACGGTT TTCAGACGTG TCGCGCGCTC AAGGACAATC CCGTCACGCG GGATATTCCC GTGATCTTCA TGACCTCGCT AACGCAGACA GAGGACAAGA TCATGGGCTT CCGCGTCGGC GCGATGGATT TCGTGACCAA GCCGCTGCAA ATGGAAGAAG TCGCTGTGCG CGTGCAGACG CATCTCAAGC TGCGTGCGTT GCAAAGGTTG CAGCAGGAAC AGAATGCCCG GCTGGAAGAG GAGATCAAGA CGCGCATTCA GGCGCAGGAT GCGCTGATCG AAGTGCTGAA CGGGGTGCGC AACGTGTCAA ACGCCATCGC GCACGATCTC CGGACGCCGC TCACGGAGCT GCGATCGAGA CTCGAGGTGC TGCTGCTCGG CTTGCGCAAG AAGGGCGACG AAGAGACGCT GGGCCAGCTC GAAGTGGCGA TGGCCGACGT GGACCGCGTG ATCGGCATTT TCAATGCGCT GCTGCGTCTC GCCGAGATCG ATGCGGGCGT GCGGCGCTCG GGTTTTGTCA GAAGCGACGT CGTGACTATC CTGTCGGACG CCGTCGAGTT CTATCAGCCC GTCGCGGAGT TACGCGGCAT TTCGCTGACG CTGTTGCTAT GTTCGGAAAG GGAGGTACTG GCCGAAGTCG ACCCGTTGCT GCTTGCGCAG GCGATCGGTA ATCTGATCGA CAACGCGCTG AAGTACGCGC AAGACAACGG CGAGGTCGAG GTGTCGCTGT GTGAGCGTAA GGATCGTATC GACGTCACGG TGTCGGATGA CGGACCCGGT ATCCCCTTCA CCGAACGGTC CAAGGTGACA GAGCGGTTCT ATCGCGGCGA CCGGAGCCGG GGAACCCCTG GCGTCGGACT CGGGCTCGCA CTCGTCAAAG CGGTGGCGAC GCTGCACCAC GGTTTTCTGG AGTTTACTGA CAACGAGCCA GGCCTTGCTG CCACCATGAC GATCCTCCGG GTCAGCTAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA