BuphA.01668.b

Histidine kinase

CENTER ID: BuphA.01668.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01668.b.A1.GE32299 Full length( BuphA.01668.b ) 1 547
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01668.b.A1.PS01240 Full length( BuphA.01668.b ) 1 547

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.145
RefSeq: YP_001856374.1
UniProt: B2JKD9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVAAPLILN VDDNDGARYA KTRVLVHAGF DVVEAATGQG ALDQVRTHNP AIVLLDVKLP DISGIEVCSI LKRDPQTRST LILQTSAAAV QSFDKVRALD GGADSYLTEP IEPAELVANV RALLRLHRAE EALRDADRRK DQFLATLAHE LRNPLAPIRN AVELMDPRYH AAEDAVCHAR MIARRQVEHL SRLVDDLLDV SRITHGKIAL QRECVHLDAA VATAVEANQP FIATKQHTLR VNVPKSACMI YGDPIRVAQV ISNLLSNAAK YTPEGGVIEL DVSAADGSVT VRVRDNGIGI PPNELADVFN LFMQSEDSLA RSEGGLGIGL SLAKTLTELH GGTIEAFSGG LGMGSEFVVT LPMVVEQSAG KPCDASPDTS AAADGEPAAR QPAGEATPAT RRRVMVVDDS VDGAESMSIL LEMLGHEVRV MYDGAAAIAA ASEFKPEVVL LDIGLPGVDG YQVARALRAE PATAGALLIA LTGYGQDTDR QRTRDAGFDH HLVKPASLDD IERVIAADGA SGAPRNPGTT LRPDANCSDA TRSDVTE
NT Sequence
ATGACGGTTG CCGCTCCGCT GATTTTGAAC GTCGACGACA ACGACGGCGC GCGTTATGCG AAAACGCGCG TGCTCGTGCA TGCCGGTTTC GATGTGGTCG AGGCGGCGAC GGGCCAGGGC GCGCTCGATC AGGTGCGCAC GCACAACCCC GCGATCGTGC TGCTCGATGT GAAGCTGCCC GATATCAGCG GCATCGAGGT CTGTTCGATT CTCAAGCGCG ATCCGCAGAC GCGATCGACG CTCATCCTGC AAACGTCAGC GGCCGCTGTG CAATCGTTCG ACAAGGTTCG CGCGCTCGAC GGCGGAGCGG ACAGCTATCT GACCGAGCCG ATCGAGCCCG CCGAACTCGT CGCCAACGTG CGCGCGCTGC TGCGGCTGCA TCGCGCGGAA GAAGCGCTGC GCGACGCCGA CCGCCGCAAG GACCAGTTTC TCGCGACGCT CGCACACGAA CTGCGCAATC CGCTCGCGCC GATCCGCAAT GCGGTCGAAC TGATGGATCC GCGCTATCAC GCGGCCGAAG ACGCCGTGTG TCATGCGCGC ATGATCGCGC GCCGTCAGGT CGAACATCTG AGCCGGCTGG TCGACGATCT GCTCGACGTG TCGCGCATCA CGCACGGCAA GATCGCGCTG CAGCGCGAAT GCGTTCATCT CGATGCTGCC GTCGCGACGG CCGTCGAAGC GAACCAGCCG TTCATCGCGA CGAAACAGCA CACATTGCGC GTCAATGTGC CCAAGAGCGC CTGCATGATT TACGGTGATC CGATCCGCGT CGCGCAGGTG ATCAGCAATC TTTTGTCGAA TGCGGCGAAG TACACGCCGG AGGGCGGCGT GATCGAACTG GACGTGAGCG CCGCCGACGG CAGCGTGACG GTTCGCGTGC GCGACAACGG CATCGGCATT CCACCGAACG AACTGGCGGA TGTGTTCAAC CTGTTCATGC AGTCAGAAGA TTCGCTGGCG CGCTCCGAAG GCGGTCTCGG CATTGGGCTG TCGCTCGCGA AGACCCTCAC CGAACTGCAT GGCGGAACGA TCGAAGCATT TTCAGGAGGA CTCGGGATGG GCTCAGAATT CGTCGTGACG CTGCCGATGG TGGTCGAACA AAGCGCTGGC AAGCCCTGTG ACGCATCGCC GGACACGTCG GCGGCGGCTG ACGGAGAGCC CGCTGCCAGG CAGCCCGCGG GCGAAGCCAC GCCCGCAACA CGACGGCGCG TGATGGTGGT CGACGACAGC GTCGATGGCG CGGAGTCGAT GTCGATCCTG CTCGAGATGC TCGGCCACGA GGTGCGCGTG ATGTACGACG GCGCAGCGGC GATCGCGGCC GCGAGCGAAT TCAAGCCGGA GGTCGTGCTG CTCGATATCG GCTTGCCGGG CGTCGACGGC TACCAGGTGG CGCGCGCGTT GCGCGCCGAG CCGGCCACGG CGGGCGCGCT GCTGATCGCG CTGACGGGCT ACGGACAGGA CACCGACCGC CAGCGTACGC GCGACGCGGG CTTCGATCAC CACCTCGTGA AGCCGGCTTC GCTCGACGAC ATCGAGCGCG TGATCGCGGC GGACGGCGCA AGCGGAGCGC CGCGCAATCC CGGCACGACG CTGCGGCCGG ACGCGAACTG CTCTGACGCG ACCCGTTCCG ATGTGACGGA G
Details for BuphA.01668.b.A1.GE32299
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 60kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MTVAAPLILN VDDNDGARYA KTRVLVHAGF DVVEAATGQG ALDQVRTHNP AIVLLDVKLP DISGIEVCSI LKRDPQTRST LILQTSAAAV QSFDKVRALD GGADSYLTEP IEPAELVANV RALLRLHRAE EALRDADRRK DQFLATLAHE LRNPLAPIRN AVELMDPRYH AAEDAVCHAR MIARRQVEHL SRLVDDLLDV SRITHGKIAL QRECVHLDAA VATAVEANQP FIATKQHTLR VNVPKSACMI YGDPIRVAQV ISNLLSNAAK YTPEGGVIEL DVSAADGSVT VRVRDNGIGI PPNELADVFN LFMQSEDSLA RSEGGLGIGL SLAKTLTELH GGTIEAFSGG LGMGSEFVVT LPMVVEQSAG KPCDASPDTS AAADGEPAAR QPAGEATPAT RRRVMVVDDS VDGAESMSIL LEMLGHEVRV MYDGAAAIAA ASEFKPEVVL LDIGLPGVDG YQVARALRAE PATAGALLIA LTGYGQDTDR QRTRDAGFDH HLVKPASLDD IERVIAADGA SGAPRNPGTT LRPDANCSDA TRSDVTE
Validated NT Sequence
actccgtcac atcggaacgg gtcgcgtcag agcagttcgc gtccggccgc agcgtcgtgc cgggattgcg cggcgctccg cttgcgccgt ccgccgcgat cacgcgctcg atgtcgtcga gcgaagccgg cttcacgagg tggtgatcga agcccgcgtc gcgcgtacgc tggcggtcgg tgtcctgtcc gtagcccgtc agcgcgatca gcagcgcgcc cgccgtggcc ggctcggcgc gcaacgcgcg cgccacctgg tagccgtcga cgcccggcaa gccgatatcg agcagcacga cctccggctt gaattcgctc gcggccgcga tcgccgctgc gccgtcgtac atcacgcgca cctcgtggcc gagcatctcg agcaggatcg acatcgactc cgcgccatcg acgctgtcgt cgaccaccat cacgcgccgt cgtgttgcgg gcgtggcttc gcccgcgggc tgcctggcag cgggctctcc gtcagccgcc gccgacgtgt ccggcgatgc gtcacagggc ttgccagcgc tttgttcgac caccatcggc agcgtcacga cgaattctga gcccatcccg agtcctcctg aaaatgcttc gatcgttccg ccatgcagtt cggtgagggt cttcgcgagc gacagcccaa tgccgagacc gccttcggag cgcgccagcg aatcttctga ctgcatgaac aggttgaaca catccgccag ttcgttcggt ggaatgccga tgccgttgtc gcgcacgcga accgtcacgc tgccgtcggc ggcgctcacg tccagttcga tcacgccgcc ctccggcgtg tacttcgccg cattcgacaa aagattgctg atcacctgcg cgacgcggat cggatcaccg taaatcatgc aggcgctctt gggcacattg acgcgcaatg tgtgctgttt cgtcgcgatg aacggctggt tcgcttcgac ggcngtcgcg acggcagcat cgagatgaac gcattcgcgc tgcagcgcga tcttgccgtg cgtgatgcgc gacacgtcga gcagatcgtc gaccagccgg ctcagatgtt cgacctgacg gcgcgcgatc atgcgcgcat gacacacggc gtcttcggcc gcgtgatagc gcggatccat cagttcgacc gcattgcgga tcggcgcgag cggattgcgc agttcgtgtg cgagcgtcgc gagaaactgg tccttgcggc ggtcggcgtc gcgcagcgct tcttccgcgc gatgcagccg cagcagcgcg cgcacgttgg cgacgagttc ggcgggctcg atcggctcgg tcagatagct gtccgctccg ccgtcgagcg cgcgaacctt gtcgaacgat tgcacagcgg ccgctgacgt ttgcaggatg agcgtcgatc gcgtctgcgg atcgcgcttg agaatcgaac agacctcgat gccgctgata tcgggcagct tcacatcgag cagcacgatc gcggggttgt gcgtgcgcac ctgatcgagc gcgccctggc ccgtcgccgc ctcgaccaca tcgaaaccgg catgcacgag cacgcgcgtt ttcgcataac gcgcgccgtc gttgtcgtcg acgttcaaaa tcagcggagc ggcaaccgtc atcgaaccag gaccctgggt ctgagcttcc agg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVAAPLIL NVDDNDGARY AKTRVLVHAG FDVVEAATGQ GALDQVRTHN PAIVLLDVKL PDISGIEVCS ILKRDPQTRS TLILQTSAAA VQSFDKVRAL DGGADSYLTE PIEPAELVAN VRALLRLHRA EEALRDADRR KDQFLATLAH ELRNPLAPIR NAVELMDPRY HAAEDAVCHA RMIARRQVEH LSRLVDDLLD VSRITHGKIA LQRECVHLDA AVATAVEANQ PFIATKQHTL RVNVPKSACM IYGDPIRVAQ VISNLLSNAA KYTPEGGVIE LDVSAADGSV TVRVRDNGIG IPPNELADVF NLFMQSEDSL ARSEGGLGIG LSLAKTLTEL HGGTIEAFSG GLGMGSEFVV TLPMVVEQSA GKPCDASPDT SAAADGEPAA RQPAGEATPA TRRRVMVVDD SVDGAESMSI LLEMLGHEVR VMYDGAAAIA AASEFKPEVV LLDIGLPGVD GYQVARALRA EPATAGALLI ALTGYGQDTD RQRTRDAGFD HHLVKPASLD DIERVIAADG ASGAPRNPGT TLRPDANCSD ATRSDVTE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggttgcc gctccgctga ttttgaacgt cgacgacaac gacggcgcgc gttatgcgaa aacgcgcgtg ctcgtgcatg ccggtttcga tgtggtcgag gcggcgacgg gccagggcgc gctcgatcag gtgcgcacgc acaaccccgc gatcgtgctg ctcgatgtga agctgcccga tatcagcggc atcgaggtct gttcgattct caagcgcgat ccgcagacgc gatcgacgct catcctgcaa acgtcagcgg ccgctgtgca atcgttcgac aaggttcgcg cgctcgacgg cggagcggac agctatctga ccgagccgat cgagcccgcc gaactcgtcg ccaacgtgcg cgcgctgctg cggctgcatc gcgcggaaga agcgctgcgc gacgccgacc gccgcaagga ccagtttctc gcgacgctcg cacacgaact gcgcaatccg ctcgcgccga tccgcaatgc ggtcgaactg atggatccgc gctatcacgc ggccgaagac gccgtgtgtc atgcgcgcat gatcgcgcgc cgtcaggtcg aacatctgag ccggctggtc gacgatctgc tcgacgtgtc gcgcatcacg cacggcaaga tcgcgctgca gcgcgaatgc gttcatctcg atgctgccgt cgcgacggcc gtcgaagcga accagccgtt catcgcgacg aaacagcaca cattgcgcgt caatgtgccc aagagcgcct gcatgattta cggtgatccg atccgcgtcg cgcaggtgat cagcaatctt ttgtcgaatg cggcgaagta cacgccggag ggcggcgtga tcgaactgga cgtgagcgcc gccgacggca gcgtgacggt tcgcgtgcgc gacaacggca tcggcattcc accgaacgaa ctggcggatg tgttcaacct gttcatgcag tcagaagatt cgctggcgcg ctccgaaggc ggtctcggca ttgggctgtc gctcgcgaag accctcaccg aactgcatgg cggaacgatc gaagcatttt caggaggact cgggatgggc tcagaattcg tcgtgacgct gccgatggtg gtcgaacaaa gcgctggcaa gccctgtgac gcatcgccgg acacgtcggc ggcggctgac ggagagcccg ctgccaggca gcccgcgggc gaagccacgc ccgcaacacg acggcgcgtg atggtggtcg acgacagcgt cgatggcgcg gagtcgatgt cgatcctgct cgagatgctc ggccacgagg tgcgcgtgat gtacgacggc gcagcggcga tcgcggccgc gagcgaattc aagccggagg tcgtgctgct cgatatcggc ttgccgggcg tcgacggcta ccaggtggcg cgcgcgttgc gcgccgagcc ggccacggcg ggcgcgctgc tgatcgcgct gacgggctac ggacaggaca ccgaccgcca gcgtacgcgc gacgcgggct tcgatcacca cctcgtgaag ccggcttcgc tcgacgacat cgagcgcgtg atcgcggcgg acggcgcaag cggagcgccg cgcaatcccg gcacgacgct gcggccggac gcgaactgct ctgacgcgac ccgttccgat gtgacggagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BuphA.01668.b.A1.PS01240
PURIFICATION DATe: 10/12/2011
CONCENTRATION: 47.6mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
notes unavailable
Validated AA Sequence
MTVAAPLILN VDDNDGARYA KTRVLVHAGF DVVEAATGQG ALDQVRTHNP AIVLLDVKLP DISGIEVCSI LKRDPQTRST LILQTSAAAV QSFDKVRALD GGADSYLTEP IEPAELVANV RALLRLHRAE EALRDADRRK DQFLATLAHE LRNPLAPIRN AVELMDPRYH AAEDAVCHAR MIARRQVEHL SRLVDDLLDV SRITHGKIAL QRECVHLDAA VATAVEANQP FIATKQHTLR VNVPKSACMI YGDPIRVAQV ISNLLSNAAK YTPEGGVIEL DVSAADGSVT VRVRDNGIGI PPNELADVFN LFMQSEDSLA RSEGGLGIGL SLAKTLTELH GGTIEAFSGG LGMGSEFVVT LPMVVEQSAG KPCDASPDTS AAADGEPAAR QPAGEATPAT RRRVMVVDDS VDGAESMSIL LEMLGHEVRV MYDGAAAIAA ASEFKPEVVL LDIGLPGVDG YQVARALRAE PATAGALLIA LTGYGQDTDR QRTRDAGFDH HLVKPASLDD IERVIAADGA SGAPRNPGTT LRPDANCSDA TRSDVTE
Validated NT Sequence
actccgtcac atcggaacgg gtcgcgtcag agcagttcgc gtccggccgc agcgtcgtgc cgggattgcg cggcgctccg cttgcgccgt ccgccgcgat cacgcgctcg atgtcgtcga gcgaagccgg cttcacgagg tggtgatcga agcccgcgtc gcgcgtacgc tggcggtcgg tgtcctgtcc gtagcccgtc agcgcgatca gcagcgcgcc cgccgtggcc ggctcggcgc gcaacgcgcg cgccacctgg tagccgtcga cgcccggcaa gccgatatcg agcagcacga cctccggctt gaattcgctc gcggccgcga tcgccgctgc gccgtcgtac atcacgcgca cctcgtggcc gagcatctcg agcaggatcg acatcgactc cgcgccatcg acgctgtcgt cgaccaccat cacgcgccgt cgtgttgcgg gcgtggcttc gcccgcgggc tgcctggcag cgggctctcc gtcagccgcc gccgacgtgt ccggcgatgc gtcacagggc ttgccagcgc tttgttcgac caccatcggc agcgtcacga cgaattctga gcccatcccg agtcctcctg aaaatgcttc gatcgttccg ccatgcagtt cggtgagggt cttcgcgagc gacagcccaa tgccgagacc gccttcggag cgcgccagcg aatcttctga ctgcatgaac aggttgaaca catccgccag ttcgttcggt ggaatgccga tgccgttgtc gcgcacgcga accgtcacgc tgccgtcggc ggcgctcacg tccagttcga tcacgccgcc ctccggcgtg tacttcgccg cattcgacaa aagattgctg atcacctgcg cgacgcggat cggatcaccg taaatcatgc aggcgctctt gggcacattg acgcgcaatg tgtgctgttt cgtcgcgatg aacggctggt tcgcttcgac ggcngtcgcg acggcagcat cgagatgaac gcattcgcgc tgcagcgcga tcttgccgtg cgtgatgcgc gacacgtcga gcagatcgtc gaccagccgg ctcagatgtt cgacctgacg gcgcgcgatc atgcgcgcat gacacacggc gtcttcggcc gcgtgatagc gcggatccat cagttcgacc gcattgcgga tcggcgcgag cggattgcgc agttcgtgtg cgagcgtcgc gagaaactgg tccttgcggc ggtcggcgtc gcgcagcgct tcttccgcgc gatgcagccg cagcagcgcg cgcacgttgg cgacgagttc ggcgggctcg atcggctcgg tcagatagct gtccgctccg ccgtcgagcg cgcgaacctt gtcgaacgat tgcacagcgg ccgctgacgt ttgcaggatg agcgtcgatc gcgtctgcgg atcgcgcttg agaatcgaac agacctcgat gccgctgata tcgggcagct tcacatcgag cagcacgatc gcggggttgt gcgtgcgcac ctgatcgagc gcgccctggc ccgtcgccgc ctcgaccaca tcgaaaccgg catgcacgag cacgcgcgtt ttcgcataac gcgcgccgtc gttgtcgtcg acgttcaaaa tcagcggagc ggcaaccgtc atcgaaccag gaccctgggt ctgagcttcc agg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTVAAPLIL NVDDNDGARY AKTRVLVHAG FDVVEAATGQ GALDQVRTHN PAIVLLDVKL PDISGIEVCS ILKRDPQTRS TLILQTSAAA VQSFDKVRAL DGGADSYLTE PIEPAELVAN VRALLRLHRA EEALRDADRR KDQFLATLAH ELRNPLAPIR NAVELMDPRY HAAEDAVCHA RMIARRQVEH LSRLVDDLLD VSRITHGKIA LQRECVHLDA AVATAVEANQ PFIATKQHTL RVNVPKSACM IYGDPIRVAQ VISNLLSNAA KYTPEGGVIE LDVSAADGSV TVRVRDNGIG IPPNELADVF NLFMQSEDSL ARSEGGLGIG LSLAKTLTEL HGGTIEAFSG GLGMGSEFVV TLPMVVEQSA GKPCDASPDT SAAADGEPAA RQPAGEATPA TRRRVMVVDD SVDGAESMSI LLEMLGHEVR VMYDGAAAIA AASEFKPEVV LLDIGLPGVD GYQVARALRA EPATAGALLI ALTGYGQDTD RQRTRDAGFD HHLVKPASLD DIERVIAADG ASGAPRNPGT TLRPDANCSD ATRSDVTE
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGGTTGCC GCTCCGCTGA TTTTGAACGT CGACGACAAC GACGGCGCGC GTTATGCGAA AACGCGCGTG CTCGTGCATG CCGGTTTCGA TGTGGTCGAG GCGGCGACGG GCCAGGGCGC GCTCGATCAG GTGCGCACGC ACAACCCCGC GATCGTGCTG CTCGATGTGA AGCTGCCCGA TATCAGCGGC ATCGAGGTCT GTTCGATTCT CAAGCGCGAT CCGCAGACGC GATCGACGCT CATCCTGCAA ACGTCAGCGG CCGCTGTGCA ATCGTTCGAC AAGGTTCGCG CGCTCGACGG CGGAGCGGAC AGCTATCTGA CCGAGCCGAT CGAGCCCGCC GAACTCGTCG CCAACGTGCG CGCGCTGCTG CGGCTGCATC GCGCGGAAGA AGCGCTGCGC GACGCCGACC GCCGCAAGGA CCAGTTTCTC GCGACGCTCG CACACGAACT GCGCAATCCG CTCGCGCCGA TCCGCAATGC GGTCGAACTG ATGGATCCGC GCTATCACGC GGCCGAAGAC GCCGTGTGTC ATGCGCGCAT GATCGCGCGC CGTCAGGTCG AACATCTGAG CCGGCTGGTC GACGATCTGC TCGACGTGTC GCGCATCACG CACGGCAAGA TCGCGCTGCA GCGCGAATGC GTTCATCTCG ATGCTGCCGT CGCGACGGCC GTCGAAGCGA ACCAGCCGTT CATCGCGACG AAACAGCACA CATTGCGCGT CAATGTGCCC AAGAGCGCCT GCATGATTTA CGGTGATCCG ATCCGCGTCG CGCAGGTGAT CAGCAATCTT TTGTCGAATG CGGCGAAGTA CACGCCGGAG GGCGGCGTGA TCGAACTGGA CGTGAGCGCC GCCGACGGCA GCGTGACGGT TCGCGTGCGC GACAACGGCA TCGGCATTCC ACCGAACGAA CTGGCGGATG TGTTCAACCT GTTCATGCAG TCAGAAGATT CGCTGGCGCG CTCCGAAGGC GGTCTCGGCA TTGGGCTGTC GCTCGCGAAG ACCCTCACCG AACTGCATGG CGGAACGATC GAAGCATTTT CAGGAGGACT CGGGATGGGC TCAGAATTCG TCGTGACGCT GCCGATGGTG GTCGAACAAA GCGCTGGCAA GCCCTGTGAC GCATCGCCGG ACACGTCGGC GGCGGCTGAC GGAGAGCCCG CTGCCAGGCA GCCCGCGGGC GAAGCCACGC CCGCAACACG ACGGCGCGTG ATGGTGGTCG ACGACAGCGT CGATGGCGCG GAGTCGATGT CGATCCTGCT CGAGATGCTC GGCCACGAGG TGCGCGTGAT GTACGACGGC GCAGCGGCGA TCGCGGCCGC GAGCGAATTC AAGCCGGAGG TCGTGCTGCT CGATATCGGC TTGCCGGGCG TCGACGGCTA CCAGGTGGCG CGCGCGTTGC GCGCCGAGCC GGCCACGGCG GGCGCGCTGC TGATCGCGCT GACGGGCTAC GGACAGGACA CCGACCGCCA GCGTACGCGC GACGCGGGCT TCGATCACCA CCTCGTGAAG CCGGCTTCGC TCGACGACAT CGAGCGCGTG ATCGCGGCGG ACGGCGCAAG CGGAGCGCCG CGCAATCCCG GCACGACGCT GCGGCCGGAC GCGAACTGCT CTGACGCGAC CCGTTCCGAT GTGACGGAGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA