BuphA.01664.a
Histidine kinase
| CENTER ID: | BuphA.01664.a |
| ORGANISM: | Burkholderia phymatum STM815 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuphA.01664.a.A1.GE32164 | Full length( BuphA.01664.a ) | 1 | 266 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6NB0
DEPOSITED: 12/6/2018
DETERMINATION: XRay
CLONE: BuphA.01664.a.A1.GE32164
PROTEIN: BuphA.01664.a.A1.PW32082
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|391038.7.peg.4634 |
| RefSeq: | YP_001860546.1 |
| UniProt: | B2JQF9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSIVTPSTPS SSGSNEEMPA VTERTARRSA ETALFMRDHV LSLVSHDLRS PLNAIHSWAY VLDRKIDAKD TTAQRALEGI RNGVDQQVKL LESIVDTTRA ETKALALKRA PFALRPLLDE TIGDVREGLA ARRGIVLALN TPLAAQQMDG DRERLAAALW LLVTFAVEAS ASGTTVTLDA DVDTATLRAT VSWQATPAAL TDPALPHVLE NFARAQATHP REAGRISWVL ALCKRVAEAH DGAFEQGEFA DGQSSTLKFR ASLAGA
NT Sequence
ATGTCGATCG TGACCCCGTC TACCCCGTCT TCTTCCGGCA GCAACGAGGA GATGCCCGCT GTCACCGAGC GAACCGCGCG ACGCAGCGCC GAAACGGCGC TCTTTATGCG CGATCACGTG CTGTCGCTCG TGTCGCACGA CCTGCGCAGT CCGCTGAACG CCATTCATAG CTGGGCTTAT GTGCTCGACC GCAAGATCGA TGCAAAGGAC ACCACCGCGC AACGCGCGCT GGAAGGCATT CGCAACGGTG TCGACCAGCA GGTGAAGCTG CTCGAATCGA TCGTCGATAC GACGCGCGCA GAGACGAAGG CGCTCGCGTT GAAGCGCGCG CCGTTCGCGC TGCGTCCATT GCTCGACGAA ACCATCGGCG ACGTGCGCGA AGGGCTCGCC GCGCGACGCG GGATCGTGCT CGCGCTGAAT ACGCCGCTCG CTGCACAGCA GATGGACGGT GATCGGGAAC GTCTCGCCGC TGCGTTGTGG CTGCTCGTCA CGTTCGCCGT AGAAGCGAGC GCGAGCGGCA CGACGGTCAC GCTGGACGCA GACGTCGATA CCGCGACGCT GCGCGCCACC GTTTCCTGGC AGGCGACCCC TGCCGCGCTG ACGGACCCGG CGCTGCCTCA TGTGCTCGAA AATTTTGCGC GCGCGCAAGC CACGCACCCT CGCGAAGCCG GCCGCATTTC GTGGGTGCTG GCGCTCTGCA AGCGCGTTGC CGAAGCGCAC GACGGTGCGT TCGAACAGGG CGAATTTGCC GACGGCCAAT CTTCCACGCT CAAGTTTCGC GCGTCGCTCG CGGGCGCG
Details for BuphA.01664.a.A1.GE32164
| HARVESTED ON: | 4/26/2011 |
| SEQUENCED ON: | 5/2/2011 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 32kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MXXHHXXHMG TLEAQTQGPG SMSIVTPSTP SSSGSNEEMP AVTERTARRS AETALFMRDH VLSLVSHDLR SPLNAIHSWA YVLDRKIDAK DTTAQRALEG IRNGVDQQVK LLESIVDTTR AETKALALKR APFALRPLLD ETIGDVREGL AARRGIVLAL NTPLAAQQMD GDRERLAAAL WLLVTFAVEA SASGTTVTLD ADVDTATLRA TVSWQATPAA LTDPALPHVL ENFARAQATH PREAGRISWV LALCKRVAEA HDGAFEQGEF ADGQSSTLKF RASLAGA
Validated NT Sequence
tattacgcgc ccgcgagcga cgcgcgaaac ttgagcgtgg aagattggcc gtcggcaaat tcgccctgtt cgaacgcacc gtcgtgcgct tcggcaacgc gcttgcagag cgccagcacc cacgaaatgc ggccggcttc gcgagggtgc gtggcttgcg cgcgcgcaaa attttcgagc acatgaggca gcgccgggtc cgtcagcgcg gcaggggtcg cctgccagga aacggtggcg cgcagcgtcg cggtatcgac gtctgcgtcc agcgtgaccg tcgtgccgct cgcgctcgct tctacggcga acgtgacgag cagccacaac gcagcggcga gacgttcccg atcaccgtcc atctgctgtg cagcgagcgg cgtattcagc gcgagcacga tcccgcgtcg cgcggcgagc ccttcgcgca cgtcgccgat ggtttcgtcg agcaatggac gcagcgcgaa cggcgcgcgc ttcaacgcga gcgccttcgt ctctgcgcgc gtcgtatcga cgatcgattc gagcagcttc acctgctggt cgacaccgtt gcgaatgcct tccagcgcgc gttgcgcggt ggtgtccttt gcatcgatct tgcggtcgag cacataagcc cagctatgaa tggcgttcag cggactgcgc aggtcgtgcg acacgagcga cagcacgtga tcgcgcataa agagcgccgt ttcggcgctg cgtcgcgcgg ttcgctcggt gacagcgggc atctcctcgt tgctgccgga agaagacggg gtagacgggg tcacgatcga catcgaacca ggaccctggg tctgagcttc cagggtaccc atatgatngt natggtgang anccatgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSIVTPSTP SSSGSNEEMP AVTERTARRS AETALFMRDH VLSLVSHDLR SPLNAIHSWA YVLDRKIDAK DTTAQRALEG IRNGVDQQVK LLESIVDTTR AETKALALKR APFALRPLLD ETIGDVREGL AARRGIVLAL NTPLAAQQMD GDRERLAAAL WLLVTFAVEA SASGTTVTLD ADVDTATLRA TVSWQATPAA LTDPALPHVL ENFARAQATH PREAGRISWV LALCKRVAEA HDGAFEQGEF ADGQSSTLKF RASLAGA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcgatcgtg accccgtcta ccccgtcttc ttccggcagc aacgaggaga tgcccgctgt caccgagcga accgcgcgac gcagcgccga aacggcgctc tttatgcgcg atcacgtgct gtcgctcgtg tcgcacgacc tgcgcagtcc gctgaacgcc attcatagct gggcttatgt gctcgaccgc aagatcgatg caaaggacac caccgcgcaa cgcgcgctgg aaggcattcg caacggtgtc gaccagcagg tgaagctgct cgaatcgatc gtcgatacga cgcgcgcaga gacgaaggcg ctcgcgttga agcgcgcgcc gttcgcgctg cgtccattgc tcgacgaaac catcggcgac gtgcgcgaag ggctcgccgc gcgacgcggg atcgtgctcg cgctgaatac gccgctcgct gcacagcaga tggacggtga tcgggaacgt ctcgccgctg cgttgtggct gctcgtcacg ttcgccgtag aagcgagcgc gagcggcacg acggtcacgc tggacgcaga cgtcgatacc gcgacgctgc gcgccaccgt ttcctggcag gcgacccctg ccgcgctgac ggacccggcg ctgcctcatg tgctcgaaaa ttttgcgcgc gcgcaagcca cgcaccctcg cgaagccggc cgcatttcgt gggtgctggc gctctgcaag cgcgttgccg aagcgcacga cggtgcgttc gaacagggcg aatttgccga cggccaatct tccacgctca agtttcgcgc gtcgctcgcg ggcgcgtaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa