BuphA.01136.a

1-deoxy-D-xylulose 5-phosphate reductoisomerase (IspC)

CENTER ID: BuphA.01136.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01136.a.A1.GE32183 Full length( BuphA.01136.a ) 1 401
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.01136.a.A1.PS01210 Full length( BuphA.01136.a ) 1 401

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1416
RefSeq: YP_001857560.1
UniProt: B2JIC0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQKRLTLLGS TGSIGDSTLD VVARHPDRFF VYALTAHRNG DKLVEQCLRF QPEVAVVGDA DTAAKVAAKL RAAGCKTAVA YGPQALVDVS KSDGCDTVVA AIVGAAGLEP SLAAARAGKR ILLANKEALV MSGSIFMDAV HDNGAILLPV DSEHNAIFQC LPRESALHGG VSKIILTASG GPFRTREPAS LVDVTPDEAC KHPNWVMGRK ISVDSATMMN KGLEVIEAHW LFNLPGDRID VLIHPQSVIH SLVSYADGSV LAQLGNPDMR TPIAHALAFP DRVDSGVGQL DLAQIAQLSF EKPDYTRFPC LALAMKALAE GGVASAALNA ANEIAVEAFL TRRIGFMAIA QVVDAVLNSL PNREATSLAD VVDADAAARR AAHAYIDGLP AGARLTERAV Q
NT Sequence
ATGCAAAAAC GACTTACTTT GCTAGGTTCC ACGGGCTCGA TCGGAGACAG CACGCTCGAC GTCGTCGCGC GTCATCCTGA CCGCTTCTTC GTGTACGCGC TGACGGCACA CCGAAATGGC GACAAGCTCG TCGAGCAATG CTTGCGCTTC CAGCCCGAAG TGGCCGTGGT CGGGGATGCC GACACGGCTG CGAAGGTGGC CGCGAAATTG CGCGCAGCGG GCTGCAAGAC GGCAGTTGCG TACGGTCCGC AGGCATTGGT CGACGTGTCG AAGAGCGACG GCTGCGATAC CGTGGTGGCG GCGATCGTCG GTGCGGCTGG TCTGGAGCCG AGTCTTGCGG CGGCGCGCGC CGGCAAGCGC ATCCTGCTCG CGAACAAGGA AGCGCTCGTG ATGTCCGGCT CGATCTTCAT GGACGCGGTG CACGATAACG GCGCGATCCT GTTGCCCGTC GACAGCGAGC ACAACGCCAT CTTCCAATGT CTGCCGCGCG AATCGGCGTT GCATGGCGGC GTGTCGAAGA TCATTCTCAC GGCCTCGGGC GGCCCGTTCC GCACGCGCGA GCCTGCCTCG CTCGTCGACG TGACGCCGGA CGAGGCGTGC AAGCACCCCA ACTGGGTGAT GGGCCGCAAG ATTTCGGTCG ATTCGGCAAC GATGATGAAC AAGGGTCTCG AAGTCATCGA AGCGCACTGG CTCTTCAACT TGCCGGGCGA CCGTATCGAC GTGCTGATCC ATCCGCAGAG CGTGATCCAT TCGCTCGTGT CGTACGCGGA CGGTTCGGTG CTCGCGCAAC TCGGCAATCC GGACATGCGC ACGCCCATCG CGCACGCGCT CGCGTTTCCG GATCGGGTGG ACTCTGGTGT CGGGCAACTC GATCTCGCGC AGATCGCGCA GCTGTCGTTC GAGAAGCCCG ACTACACGCG CTTCCCGTGC CTCGCGCTGG CCATGAAGGC GCTGGCGGAA GGCGGCGTCG CTAGCGCGGC GTTGAATGCC GCTAACGAGA TCGCGGTCGA AGCGTTCCTG ACGCGCCGCA TCGGCTTCAT GGCGATCGCG CAAGTGGTCG ACGCCGTGCT GAATAGCTTG CCCAACCGCG AGGCGACGAG CCTCGCCGAC GTGGTCGATG CCGACGCCGC CGCGCGCCGC GCTGCCCACG CGTACATCGA CGGATTGCCG GCGGGCGCTC GCCTTACGGA ACGCGCCGTC CAG
Details for BuphA.01136.a.A1.GE32183
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MQKRLTLLGS TGSIGDSTLD VVARHPDRFF VYALTAHRNG DKLVEQCLRF QPEVAVVGDA DTAAKVAAKL RAAGCKTAVA YGPQALVDVS KSDGCDTVVA AIVGAAGLEP SLAAARAGKR ILLANKEALV MSGSIFMDAV HDNGAILLPV DSEHNAIFQC LPRESALHGG VSKIILTASG GPFRTREPAS LVDVTPDEAC KHPNWVMGRK ISVDSATMMN KGLEVIEAHW LFNLPGDRID VLIHPQSVIH SLVSYADGSV LAQLGNPDMR TPIAHALAFP DRVDSGVGQL DLAQIAQLSF EKPDYTRFPC LALAMKALAE GGVASAALNA ANEIAVEAFL TRRIGFMAIA QVVDAVLNSL PNREATSLAD VVDADAAARR AAHAYIDGLP AGARLTERAV
Validated NT Sequence
tggacggcgc gttccgtaag gcgagcgccc gccggcaatc cgtcgatgta cgcgtgggca gcgcggcgcg cggcggcgtc ggcatcgacc acgtcggcga ggctcgtcgc ctcgcggttg ggcaagctat tcagcacggc gtcgaccact tgcgcgatcg ccatgaagcc gatgcggcgc gtcaggaacg cttcgaccgc gatctcgtta gcggcattca acgccgcgct agcgacgccg ccttccgcca gcgccttcat ggccagcgcg aggcacggga agcgcgtgta gtcgggcttc tcgaacgaca gctgcgcgat ctgcgcgaga tcgagttgcc cgacaccaga gtccacccga tccggaaacg cgagcgcgtg cgcgatgggc gtgcgcatgt ccggattgcc gagttgcgcg agcaccgaac cgtccgcgta cgacacgagc gaatggatca cgctctgcgg atggatcagc acgtcgatac ggtcgcccgg caagttgaag agccagtgcg cttcgatgac ttcgagaccc ttgttcatca tcgttgccga atcgaccgaa atcttgcggc ccatcaccca gttggggtgc ttgcacgcct cgtccggcgt cacgtcgacg agcgaggcag gctcgcgcgt gcggaacggg ccgcccgagg ccgtgagaat gatcttcgac acgccgccat gcaacgccga ttcgcgcggc agacattgga agatggcgtt gtgctcgctg tcgacgggca acaggatcgc gccgttatcg tgcaccgcgt ccatgaagat cgagccggac atcacgagcg cttccttgtt cgcgagcagg atgcgcttgc cggcgcgcgc cgccgcaaga ctcggctcca gaccagccgc accgacgatc gccgccacca cggtatcgca gccgtcgctc ttcgacacgt cgaccaatgc ctgcggaccg tacgcaactg ccgtcttgca gcccgctgcg cgcaatttcg cggccacctt cgcagccgtg tcggcatccc cgaccacggc cacttcgggc tggaagcgca agcattgctc gacgagcttg tcgccatttc ggtgtgccgt cagcgcgtac acgaagaagc ggtcaggatg acgcgcgacg acgtcgagcg tgctgtctcc gatcgagccc gtggaaccta gcaaagtaag tcgtttttgc atcgaaccag gacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQKRLTLLG STGSIGDSTL DVVARHPDRF FVYALTAHRN GDKLVEQCLR FQPEVAVVGD ADTAAKVAAK LRAAGCKTAV AYGPQALVDV SKSDGCDTVV AAIVGAAGLE PSLAAARAGK RILLANKEAL VMSGSIFMDA VHDNGAILLP VDSEHNAIFQ CLPRESALHG GVSKIILTAS GGPFRTREPA SLVDVTPDEA CKHPNWVMGR KISVDSATMM NKGLEVIEAH WLFNLPGDRI DVLIHPQSVI HSLVSYADGS VLAQLGNPDM RTPIAHALAF PDRVDSGVGQ LDLAQIAQLS FEKPDYTRFP CLALAMKALA EGGVASAALN AANEIAVEAF LTRRIGFMAI AQVVDAVLNS LPNREATSLA DVVDADAAAR RAAHAYIDGL PAGARLTERA VQ
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaaaaacga cttactttgc taggttccac gggctcgatc ggagacagca cgctcgacgt cgtcgcgcgt catcctgacc gcttcttcgt gtacgcgctg acggcacacc gaaatggcga caagctcgtc gagcaatgct tgcgcttcca gcccgaagtg gccgtggtcg gggatgccga cacggctgcg aaggtggccg cgaaattgcg cgcagcgggc tgcaagacgg cagttgcgta cggtccgcag gcattggtcg acgtgtcgaa gagcgacggc tgcgataccg tggtggcggc gatcgtcggt gcggctggtc tggagccgag tcttgcggcg gcgcgcgccg gcaagcgcat cctgctcgcg aacaaggaag cgctcgtgat gtccggctcg atcttcatgg acgcggtgca cgataacggc gcgatcctgt tgcccgtcga cagcgagcac aacgccatct tccaatgtct gccgcgcgaa tcggcgttgc atggcggcgt gtcgaagatc attctcacgg cctcgggcgg cccgttccgc acgcgcgagc ctgcctcgct cgtcgacgtg acgccggacg aggcgtgcaa gcaccccaac tgggtgatgg gccgcaagat ttcggtcgat tcggcaacga tgatgaacaa gggtctcgaa gtcatcgaag cgcactggct cttcaacttg ccgggcgacc gtatcgacgt gctgatccat ccgcagagcg tgatccattc gctcgtgtcg tacgcggacg gttcggtgct cgcgcaactc ggcaatccgg acatgcgcac gcccatcgcg cacgcgctcg cgtttccgga tcgggtggac tctggtgtcg ggcaactcga tctcgcgcag atcgcgcagc tgtcgttcga gaagcccgac tacacgcgct tcccgtgcct cgcgctggcc atgaaggcgc tggcggaagg cggcgtcgct agcgcggcgt tgaatgccgc taacgagatc gcggtcgaag cgttcctgac gcgccgcatc ggcttcatgg cgatcgcgca agtggtcgac gccgtgctga atagcttgcc caaccgcgag gcgacgagcc tcgccgacgt ggtcgatgcc gacgccgccg cgcgccgcgc tgcccacgcg tacatcgacg gattgccggc gggcgctcgc cttacggaac gcgccgtcca gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BuphA.01136.a.A1.PS01210
PURIFICATION DATe: 9/7/2011
CONCENTRATION: 24.25mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MQKRLTLLGS TGSIGDSTLD VVARHPDRFF VYALTAHRNG DKLVEQCLRF QPEVAVVGDA DTAAKVAAKL RAAGCKTAVA YGPQALVDVS KSDGCDTVVA AIVGAAGLEP SLAAARAGKR ILLANKEALV MSGSIFMDAV HDNGAILLPV DSEHNAIFQC LPRESALHGG VSKIILTASG GPFRTREPAS LVDVTPDEAC KHPNWVMGRK ISVDSATMMN KGLEVIEAHW LFNLPGDRID VLIHPQSVIH SLVSYADGSV LAQLGNPDMR TPIAHALAFP DRVDSGVGQL DLAQIAQLSF EKPDYTRFPC LALAMKALAE GGVASAALNA ANEIAVEAFL TRRIGFMAIA QVVDAVLNSL PNREATSLAD VVDADAAARR AAHAYIDGLP AGARLTERAV
Validated NT Sequence
tggacggcgc gttccgtaag gcgagcgccc gccggcaatc cgtcgatgta cgcgtgggca gcgcggcgcg cggcggcgtc ggcatcgacc acgtcggcga ggctcgtcgc ctcgcggttg ggcaagctat tcagcacggc gtcgaccact tgcgcgatcg ccatgaagcc gatgcggcgc gtcaggaacg cttcgaccgc gatctcgtta gcggcattca acgccgcgct agcgacgccg ccttccgcca gcgccttcat ggccagcgcg aggcacggga agcgcgtgta gtcgggcttc tcgaacgaca gctgcgcgat ctgcgcgaga tcgagttgcc cgacaccaga gtccacccga tccggaaacg cgagcgcgtg cgcgatgggc gtgcgcatgt ccggattgcc gagttgcgcg agcaccgaac cgtccgcgta cgacacgagc gaatggatca cgctctgcgg atggatcagc acgtcgatac ggtcgcccgg caagttgaag agccagtgcg cttcgatgac ttcgagaccc ttgttcatca tcgttgccga atcgaccgaa atcttgcggc ccatcaccca gttggggtgc ttgcacgcct cgtccggcgt cacgtcgacg agcgaggcag gctcgcgcgt gcggaacggg ccgcccgagg ccgtgagaat gatcttcgac acgccgccat gcaacgccga ttcgcgcggc agacattgga agatggcgtt gtgctcgctg tcgacgggca acaggatcgc gccgttatcg tgcaccgcgt ccatgaagat cgagccggac atcacgagcg cttccttgtt cgcgagcagg atgcgcttgc cggcgcgcgc cgccgcaaga ctcggctcca gaccagccgc accgacgatc gccgccacca cggtatcgca gccgtcgctc ttcgacacgt cgaccaatgc ctgcggaccg tacgcaactg ccgtcttgca gcccgctgcg cgcaatttcg cggccacctt cgcagccgtg tcggcatccc cgaccacggc cacttcgggc tggaagcgca agcattgctc gacgagcttg tcgccatttc ggtgtgccgt cagcgcgtac acgaagaagc ggtcaggatg acgcgcgacg acgtcgagcg tgctgtctcc gatcgagccc gtggaaccta gcaaagtaag tcgtttttgc atcgaaccag gacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQKRLTLLG STGSIGDSTL DVVARHPDRF FVYALTAHRN GDKLVEQCLR FQPEVAVVGD ADTAAKVAAK LRAAGCKTAV AYGPQALVDV SKSDGCDTVV AAIVGAAGLE PSLAAARAGK RILLANKEAL VMSGSIFMDA VHDNGAILLP VDSEHNAIFQ CLPRESALHG GVSKIILTAS GGPFRTREPA SLVDVTPDEA CKHPNWVMGR KISVDSATMM NKGLEVIEAH WLFNLPGDRI DVLIHPQSVI HSLVSYADGS VLAQLGNPDM RTPIAHALAF PDRVDSGVGQ LDLAQIAQLS FEKPDYTRFP CLALAMKALA EGGVASAALN AANEIAVEAF LTRRIGFMAI AQVVDAVLNS LPNREATSLA DVVDADAAAR RAAHAYIDGL PAGARLTERA VQ
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAAAAACGA CTTACTTTGC TAGGTTCCAC GGGCTCGATC GGAGACAGCA CGCTCGACGT CGTCGCGCGT CATCCTGACC GCTTCTTCGT GTACGCGCTG ACGGCACACC GAAATGGCGA CAAGCTCGTC GAGCAATGCT TGCGCTTCCA GCCCGAAGTG GCCGTGGTCG GGGATGCCGA CACGGCTGCG AAGGTGGCCG CGAAATTGCG CGCAGCGGGC TGCAAGACGG CAGTTGCGTA CGGTCCGCAG GCATTGGTCG ACGTGTCGAA GAGCGACGGC TGCGATACCG TGGTGGCGGC GATCGTCGGT GCGGCTGGTC TGGAGCCGAG TCTTGCGGCG GCGCGCGCCG GCAAGCGCAT CCTGCTCGCG AACAAGGAAG CGCTCGTGAT GTCCGGCTCG ATCTTCATGG ACGCGGTGCA CGATAACGGC GCGATCCTGT TGCCCGTCGA CAGCGAGCAC AACGCCATCT TCCAATGTCT GCCGCGCGAA TCGGCGTTGC ATGGCGGCGT GTCGAAGATC ATTCTCACGG CCTCGGGCGG CCCGTTCCGC ACGCGCGAGC CTGCCTCGCT CGTCGACGTG ACGCCGGACG AGGCGTGCAA GCACCCCAAC TGGGTGATGG GCCGCAAGAT TTCGGTCGAT TCGGCAACGA TGATGAACAA GGGTCTCGAA GTCATCGAAG CGCACTGGCT CTTCAACTTG CCGGGCGACC GTATCGACGT GCTGATCCAT CCGCAGAGCG TGATCCATTC GCTCGTGTCG TACGCGGACG GTTCGGTGCT CGCGCAACTC GGCAATCCGG ACATGCGCAC GCCCATCGCG CACGCGCTCG CGTTTCCGGA TCGGGTGGAC TCTGGTGTCG GGCAACTCGA TCTCGCGCAG ATCGCGCAGC TGTCGTTCGA GAAGCCCGAC TACACGCGCT TCCCGTGCCT CGCGCTGGCC ATGAAGGCGC TGGCGGAAGG CGGCGTCGCT AGCGCGGCGT TGAATGCCGC TAACGAGATC GCGGTCGAAG CGTTCCTGAC GCGCCGCATC GGCTTCATGG CGATCGCGCA AGTGGTCGAC GCCGTGCTGA ATAGCTTGCC CAACCGCGAG GCGACGAGCC TCGCCGACGT GGTCGATGCC GACGCCGCCG CGCGCCGCGC TGCCCACGCG TACATCGACG GATTGCCGGC GGGCGCTCGC CTTACGGAAC GCGCCGTCCA GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA