BuphA.00863.j

Histidine kinase

CENTER ID: BuphA.00863.j
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00863.j.A1.GE32292 Full length( BuphA.00863.j ) 1 524
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.6296
RefSeq: YP_001862084.1
UniProt: B2JVR1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEERILIVAP RGRDAEVVAQ VLSRERMECV VCTNVGQLVA TLPEGAACAL IADEALDALA LRSLDGWLST QPPWSDFPFV LLVGNGKKGV PTAEGRRLEV LGNVMLIERP VSGEALVSAA RSARRARRRQ YQARAMIAER AAANLRLVNA ARQKDEFLAM LAHELRNPLA PIRNAAEAIR TTDAVLPARV QWAREIIERQ SRHLASLLED LLDVSRITTG KITLKRNIIE LGGVLAAAID VAKLALDAHG HTLVVRAPDD SIYLDADATR LAQVFGNLLD NAVKYTLDGG RIEIDVTAEE GLVTVAVSDN GTGIAPEELP EIFELFSQSN RALDRAQGGL GIGLSVVRSL IGMHGGTVRA ESAGLGLGTR IVVTLPTVDA PVQGSDRADD VEHGNTQILD VLVVDDNVDA ASSLALLLEL NGHRVRIAHD GLHALDACAD AQPDAVLLDI GLPGMDGYEV AQRLRQMVTM SHATLIAITG YGQPEDIARA RAAGFDHHLV KPVEPDTLAL LFDDVRQSRA TQLR
NT Sequence
TTGGAAGAAC GGATCCTGAT CGTCGCGCCG CGCGGACGGG ACGCGGAGGT TGTCGCACAG GTGCTGTCCC GCGAACGCAT GGAATGCGTG GTCTGCACTA ACGTCGGCCA ACTGGTCGCC ACGCTGCCCG AAGGGGCGGC TTGCGCGCTG ATCGCTGATG AAGCGCTGGA TGCACTTGCC TTGCGATCTC TCGACGGATG GTTGAGCACG CAGCCGCCCT GGTCTGACTT TCCGTTTGTG CTGCTGGTTG GCAACGGAAA GAAGGGCGTT CCGACGGCCG AGGGCCGGCG TCTCGAGGTG CTAGGAAATG TCATGCTGAT CGAGCGGCCC GTCAGCGGTG AGGCACTCGT GAGTGCGGCG CGCAGCGCCC GGCGGGCAAG GCGCAGGCAA TATCAGGCAC GGGCGATGAT CGCAGAGCGG GCCGCAGCGA ACCTGCGGCT GGTGAACGCG GCACGTCAGA AGGACGAGTT CCTTGCGATG CTCGCCCATG AGTTGCGCAA TCCGCTCGCG CCGATACGCA ATGCGGCGGA GGCGATCCGG ACAACGGATG CCGTGCTGCC GGCACGCGTG CAGTGGGCGC GCGAAATCAT CGAGCGTCAG AGCCGACATT TGGCTAGTCT TCTTGAAGAT CTACTCGACG TATCGCGCAT TACCACCGGG AAGATCACAT TGAAGCGCAA CATCATCGAG CTCGGTGGCG TGCTCGCTGC AGCGATCGAC GTGGCGAAGC TGGCGCTAGA CGCGCACGGG CACACGCTTG TCGTGCGAGC CCCGGATGAC AGCATATATC TGGACGCCGA TGCAACGCGC CTCGCACAAG TGTTCGGCAA CCTTCTCGAC AACGCCGTGA AATACACACT CGATGGAGGC CGTATCGAGA TCGACGTGAC AGCAGAAGAA GGGCTGGTTA CTGTTGCTGT CTCAGATAAC GGAACTGGCA TCGCGCCGGA GGAGCTTCCC GAGATTTTCG AACTCTTCTC ACAATCAAAT CGAGCTTTGG ATCGTGCGCA AGGCGGCTTG GGCATTGGTT TATCGGTTGT CCGGTCGCTG ATCGGCATGC ACGGCGGGAC CGTGCGCGCC GAAAGCGCCG GACTTGGGCT TGGGACCCGT ATCGTGGTCA CGCTGCCGAC GGTCGACGCG CCGGTGCAGG GCAGCGACCG TGCGGACGAT GTCGAACATG GGAACACGCA GATTCTCGAC GTGCTTGTGG TTGACGACAA CGTCGATGCA GCGAGTTCGC TTGCGTTGTT ACTGGAATTG AATGGCCATC GCGTGCGCAT CGCGCACGAT GGGCTCCATG CACTTGATGC CTGCGCGGAT GCGCAGCCTG ATGCCGTGCT GCTGGACATC GGGCTGCCAG GGATGGACGG CTACGAAGTC GCTCAGCGCC TAAGGCAGAT GGTGACGATG TCTCACGCCA CCCTGATTGC GATCACCGGT TACGGGCAAC CCGAGGATAT TGCACGCGCC CGCGCTGCAG GCTTTGACCA TCATCTGGTC AAGCCCGTCG AACCCGACAC GCTCGCACTG TTGTTTGATG ACGTAAGACA GAGCCGCGCC ACACAGCTGC GA
Details for BuphA.00863.j.A1.GE32292
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 80
PERCENT COVERAGE: 72
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMLEERILIV APRGRDAEVV AQVLSRERME CVVCTNVGQL VATLPEGAAC ALIADEALDA LALRSLDGWL STQPPWSDFP FVLLVGNGKK GVPTAEGRRL EVLGNVMLIE RPVSGEALVS AARSARRARR RQYQARAMIA ERAAANLRLV NAARQKDEFL AMLAHELRNP LAPIRNAAXA IRTTDAVLPA RVQWAREIIE RQSRHLASLL EDLLDVSRIT TGXITLKRNI IELGGVLAAA IDVAKLALDA HGHTLVVRAP DDSIYLDADA TRLAQVFGNL LDXAXKYTLX GGXIEIDVTA EXXWLLXLSX XTELXSRXXX XPXXXNXXXX QIXXXXXXXX XXGXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXX
Validated NT Sequence
attttgttnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgttgg aagaacggat cctgatcgtc gcgccgcgcg gacgggacgc ggaggttgtc gcacaggtgc tgtcccgcga acgcatggaa tgcgtggtct gcactaacgt cggccaactg gtcgccacgc tgcccgaagg ggcggcttgc gcgctgatcg ctgatgaagc gctggatgca cttgccttgc gatctctcga cggatggttg agcacgcagc cgccctggtc tgactttccg tttgtgctgc tggttggcaa cggaaagaag ggcgttccga cggccgaggg ccggcgtctc gaggtgctag gaaatgtcat gctgatcgag cggcccgtca gcggtgaggc actcgtgagt gcggcgcgca gcgcccggcg ggcaaggcgc aggcaatatc aggcacgggc gatgatcgca gagcgggccg cagcgaacct gcggctggtg aacgcggcac gtcagaagga cgagttcctt gcgatgctcg cccatgagtt gcgcaatccg ctcgcgccga tacgcaatgc ggcggangcg atccggacaa cggatgccgt gctgccggca cgcgtgcagt gggcgcgcga aatcatcgag cgtcagagcc gacatttggc tagtcttctt gaagatctac tcgacgtatc gcgcattacc accgggnaga tcacattgaa gcgcaacatc atcgagctcg gtggcgtgct cgctgcagcg atcgacgtgg cgaagctggc gctagacgcg cacgggcaca cgcttgtcgt gcgagccccg gatgacagca tatatctgga cgccgatgca acgcgcctcg cacaagtgtt cggcaacctt ctcgacancg ccntgaaata cacactcnat ggaggcngta tcgagatcga cgtgacagca gaanannnnt ggttactgnn gctgtctcan atnacggaac tggnatcncg ccnnagnanc ntcccnannn ttncgaactn ntnnnnnnna caaatcnagn ntttnnntnn ngnnnnnnnn nncnnnggna tnnnnttnnn nnnnntnnnn nnnnntnann nnnnnnnnnn nnnnnngnnc nnngngnnnn nnaannnnnn nnnnnnnnnn nnnnnncnnn nnnnnnnnnn nnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLEERILIV APRGRDAEVV AQVLSRERME CVVCTNVGQL VATLPEGAAC ALIADEALDA LALRSLDGWL STQPPWSDFP FVLLVGNGKK GVPTAEGRRL EVLGNVMLIE RPVSGEALVS AARSARRARR RQYQARAMIA ERAAANLRLV NAARQKDEFL AMLAHELRNP LAPIRNAAEA IRTTDAVLPA RVQWAREIIE RQSRHLASLL EDLLDVSRIT TGKITLKRNI IELGGVLAAA IDVAKLALDA HGHTLVVRAP DDSIYLDADA TRLAQVFGNL LDNAVKYTLD GGRIEIDVTA EEGLVTVAVS DNGTGIAPEE LPEIFELFSQ SNRALDRAQG GLGIGLSVVR SLIGMHGGTV RAESAGLGLG TRIVVTLPTV DAPVQGSDRA DDVEHGNTQI LDVLVVDDNV DAASSLALLL ELNGHRVRIA HDGLHALDAC ADAQPDAVLL DIGLPGMDGY EVAQRLRQMV TMSHATLIAI TGYGQPEDIA RARAAGFDHH LVKPVEPDTL ALLFDDVRQS RATQLR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gttggaagaa cggatcctga tcgtcgcgcc gcgcggacgg gacgcggagg ttgtcgcaca ggtgctgtcc cgcgaacgca tggaatgcgt ggtctgcact aacgtcggcc aactggtcgc cacgctgccc gaaggggcgg cttgcgcgct gatcgctgat gaagcgctgg atgcacttgc cttgcgatct ctcgacggat ggttgagcac gcagccgccc tggtctgact ttccgtttgt gctgctggtt ggcaacggaa agaagggcgt tccgacggcc gagggccggc gtctcgaggt gctaggaaat gtcatgctga tcgagcggcc cgtcagcggt gaggcactcg tgagtgcggc gcgcagcgcc cggcgggcaa ggcgcaggca atatcaggca cgggcgatga tcgcagagcg ggccgcagcg aacctgcggc tggtgaacgc ggcacgtcag aaggacgagt tccttgcgat gctcgcccat gagttgcgca atccgctcgc gccgatacgc aatgcggcgg aggcgatccg gacaacggat gccgtgctgc cggcacgcgt gcagtgggcg cgcgaaatca tcgagcgtca gagccgacat ttggctagtc ttcttgaaga tctactcgac gtatcgcgca ttaccaccgg gaagatcaca ttgaagcgca acatcatcga gctcggtggc gtgctcgctg cagcgatcga cgtggcgaag ctggcgctag acgcgcacgg gcacacgctt gtcgtgcgag ccccggatga cagcatatat ctggacgccg atgcaacgcg cctcgcacaa gtgttcggca accttctcga caacgccgtg aaatacacac tcgatggagg ccgtatcgag atcgacgtga cagcagaaga agggctggtt actgttgctg tctcagataa cggaactggc atcgcgccgg aggagcttcc cgagattttc gaactcttct cacaatcaaa tcgagctttg gatcgtgcgc aaggcggctt gggcattggt ttatcggttg tccggtcgct gatcggcatg cacggcggga ccgtgcgcgc cgaaagcgcc ggacttgggc ttgggacccg tatcgtggtc acgctgccga cggtcgacgc gccggtgcag ggcagcgacc gtgcggacga tgtcgaacat gggaacacgc agattctcga cgtgcttgtg gttgacgaca acgtcgatgc agcgagttcg cttgcgttgt tactggaatt gaatggccat cgcgtgcgca tcgcgcacga tgggctccat gcacttgatg cctgcgcgga tgcgcagcct gatgccgtgc tgctggacat cgggctgcca gggatggacg gctacgaagt cgctcagcgc ctaaggcaga tggtgacgat gtctcacgcc accctgattg cgatcaccgg ttacgggcaa cccgaggata ttgcacgcgc ccgcgctgca ggctttgacc atcatctggt caagcccgtc gaacccgaca cgctcgcact gttgtttgat gacgtaagac agagccgcgc cacacagctg cgataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa