BuphA.00863.g

Response regulator receiver sensor signal transduction histidine kinase

CENTER ID: BuphA.00863.g
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00863.g.A1.GE32289 Full length( BuphA.00863.g ) 1 370
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4440
RefSeq: YP_001860374.1
UniProt: B2JPY7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNSPVNILI VDDIAHNITA LEALLARPGV ELLVANSGTA ALDLLLKHEA ALAILDVNMP GMNGFELASL MRGSPRTSHV PIIFLTATAE DASRTFRGYE AGAVDFLHKP FDPRILQSKV DVFVQLEQHK RQLAEQLLAT RQLLEANEML MAVLGHDLRT PLGTVLASAE YLVRNAPDEQ SGAVAGRIRN SSLRMSRMVH QLLNLARLQG GRMQLQPRPS ELATLCRGVI EEFAAHERAS QIVFTSRGNT TGNWDTDLLW QAISNLISNA LHHGERDSPI TVDVNAEESN RLRLTVSNRG AIPAAILPHL FEPFGSANAG ERSREGLGLG LHIVHKIVHM HGGKVTGASD EIFGTVFTVE LPRTAQSWVS
NT Sequence
ATGACGAATT CGCCCGTCAA CATTCTGATC GTCGACGATA TCGCGCATAA CATCACGGCA CTCGAAGCGC TGCTGGCGCG GCCCGGCGTC GAACTGCTGG TCGCGAACTC GGGGACGGCC GCGCTCGACC TGCTGCTCAA GCATGAAGCC GCGCTCGCGA TACTCGACGT CAACATGCCC GGCATGAACG GCTTCGAACT CGCGTCGCTG ATGCGCGGCA GCCCGCGCAC GTCGCACGTG CCCATCATCT TTCTGACGGC CACCGCGGAA GATGCGTCGC GCACGTTTCG CGGCTACGAA GCGGGCGCGG TCGACTTTTT GCACAAGCCA TTCGACCCGC GCATCCTGCA ATCGAAGGTC GACGTGTTCG TGCAACTGGA ACAGCACAAG CGGCAGCTCG CCGAACAGCT GCTGGCCACG CGCCAGCTGC TCGAAGCAAA CGAAATGCTG ATGGCCGTGC TCGGCCACGA TCTGCGCACG CCGCTTGGCA CGGTGCTCGC GTCGGCGGAA TACCTGGTGC GCAATGCGCC TGACGAGCAA TCCGGAGCAG TCGCGGGCCG CATCAGGAAC AGTTCGCTGC GCATGTCGCG CATGGTGCAT CAACTGCTCA ATCTCGCGCG GCTGCAAGGC GGCCGCATGC AGTTGCAGCC GCGCCCGAGC GAACTCGCGA CGCTGTGCCG CGGCGTGATC GAAGAGTTCG CGGCGCACGA GCGCGCCAGC CAGATCGTCT TTACCTCGCG CGGCAACACG ACGGGCAACT GGGACACAGA TCTGCTGTGG CAGGCCATTT CGAATCTGAT CAGCAACGCG CTGCATCACG GCGAGCGCGA CAGCCCGATC ACTGTCGACG TCAACGCGGA GGAATCGAAC CGGCTAAGAT TAACCGTTAG CAATCGCGGC GCGATTCCGG CTGCGATACT GCCGCATCTG TTCGAGCCGT TCGGATCCGC CAACGCGGGC GAACGGTCGC GCGAAGGACT CGGGCTCGGG TTGCACATCG TGCACAAAAT CGTGCACATG CACGGCGGCA AGGTGACGGG CGCGTCGGAC GAGATCTTCG GCACCGTGTT TACCGTCGAG CTGCCGCGCA CGGCGCAAAG CTGGGTGAGC
Details for BuphA.00863.g.A1.GE32289
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 85
PERCENT COVERAGE: 105
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSPVNIL IVDDIAHNIT ALEALLARPG VELLVANSGT AALDLLLKHE AALAILDVNM PGMNGFELAS LMRGSPRTSH VPIIFLTATA EDASRTFRGY EAGAVDFLHK PFDPRILQSK VDVFVQLEQH KRQLAEQLLA TRQLLEANEM LMAVLGHDLR TPLGTVLASA EYLVRNAPDE QSGAVAGRIR NSSLRMSRMV HQLLNLARLQ GGRMQLQPRP SELATLCRGV IEEFAAHERA SQIVFTSRGN TTGNWDTDLL WQAISNLISN ALHHGERDSP ITVDVNAEES NRLXLTVSNR GAIXXAILPH LXXPFXSAXX GRXVXXXXXA XXXXXXXXIX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XX
Validated NT Sequence
ttttnnttan ctttaagang gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgacga attcgcccgt caacattctg atcgtcgacg atatcgcgca taacatcacg gcactcgaag cgctgctggc gcggcccggc gtcgaactgc tggtcgcgaa ctcggggacg gccgcgctcg acctgctgct caagcatgaa gccgcgctcg cgatactcga cgtcaacatg cccggcatga acggcttcga actcgcgtcg ctgatgcgcg gcagcccgcg cacgtcgcac gtgcccatca tctttctgac ggccaccgcg gaagatgcgt cgcgcacgtt tcgcggctac gaagcgggcg cggtcgactt tttgcacaag ccattcgacc cgcgcatcct gcaatcgaag gtcgacgtgt tcgtgcaact ggaacagcac aagcggcagc tcgccgaaca gctgctggcc acgcgccagc tgctcgaagc aaacgaaatg ctgatggccg tgctcggcca cgatctgcgc acgccgcttg gcacggtgct cgcgtcggcg gaatacctgg tgcgcaatgc gcctgacgag caatccggag cagtcgcggg ccgcatcagg aacagttcgc tgcgcatgtc gcgcatggtg catcaactgc tcaatctcgc gcggctgcaa ggcggccgca tgcagttgca gccgcgcccg agcgaactcg cgacgctgtg ccgcggcgtg atcgaagagt tcgcggcgca cgagcgcgcc agccagatcg tctttacctc gcgcggcaac acgacnggca actgggacac agatctgctg tggcaggcca tttcgaatct gatcagcaac gcgctgcatc acggcgagcg cgacagcccg atcactgtcg acgtcaacgc ggaggaatcg aaccggctaa nattaaccgt tagcaatcgc ggcgcgattc nnnctgcnat actgccgcat ctgntcganc cgttcnnatc cgccnacnng ggncgnnnng tcnnnnnnnn nntnngggct cnggnnngnn nnntcnngnn nnaaatcnnn cnnnngnnnn gnnggnggnn nnnngnnnnn nnnnnnannn nancnnnnnn nnnnnnnnnn nntcnnnnnn nnnnnnnnnn nnnnnnggnn nnnnnnnngg nnnnnnnnnn annnnnnnnn nnnnnnnnnn nnnnnnnnnn nncngnnnnn nnnaannnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTNSPVNIL IVDDIAHNIT ALEALLARPG VELLVANSGT AALDLLLKHE AALAILDVNM PGMNGFELAS LMRGSPRTSH VPIIFLTATA EDASRTFRGY EAGAVDFLHK PFDPRILQSK VDVFVQLEQH KRQLAEQLLA TRQLLEANEM LMAVLGHDLR TPLGTVLASA EYLVRNAPDE QSGAVAGRIR NSSLRMSRMV HQLLNLARLQ GGRMQLQPRP SELATLCRGV IEEFAAHERA SQIVFTSRGN TTGNWDTDLL WQAISNLISN ALHHGERDSP ITVDVNAEES NRLRLTVSNR GAIPAAILPH LFEPFGSANA GERSREGLGL GLHIVHKIVH MHGGKVTGAS DEIFGTVFTV ELPRTAQSWV S
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgaattcg cccgtcaaca ttctgatcgt cgacgatatc gcgcataaca tcacggcact cgaagcgctg ctggcgcggc ccggcgtcga actgctggtc gcgaactcgg ggacggccgc gctcgacctg ctgctcaagc atgaagccgc gctcgcgata ctcgacgtca acatgcccgg catgaacggc ttcgaactcg cgtcgctgat gcgcggcagc ccgcgcacgt cgcacgtgcc catcatcttt ctgacggcca ccgcggaaga tgcgtcgcgc acgtttcgcg gctacgaagc gggcgcggtc gactttttgc acaagccatt cgacccgcgc atcctgcaat cgaaggtcga cgtgttcgtg caactggaac agcacaagcg gcagctcgcc gaacagctgc tggccacgcg ccagctgctc gaagcaaacg aaatgctgat ggccgtgctc ggccacgatc tgcgcacgcc gcttggcacg gtgctcgcgt cggcggaata cctggtgcgc aatgcgcctg acgagcaatc cggagcagtc gcgggccgca tcaggaacag ttcgctgcgc atgtcgcgca tggtgcatca actgctcaat ctcgcgcggc tgcaaggcgg ccgcatgcag ttgcagccgc gcccgagcga actcgcgacg ctgtgccgcg gcgtgatcga agagttcgcg gcgcacgagc gcgccagcca gatcgtcttt acctcgcgcg gcaacacgac gggcaactgg gacacagatc tgctgtggca ggccatttcg aatctgatca gcaacgcgct gcatcacggc gagcgcgaca gcccgatcac tgtcgacgtc aacgcggagg aatcgaaccg gctaagatta accgttagca atcgcggcgc gattccggct gcgatactgc cgcatctgtt cgagccgttc ggatccgcca acgcgggcga acggtcgcgc gaaggactcg ggctcgggtt gcacatcgtg cacaaaatcg tgcacatgca cggcggcaag gtgacgggcg cgtcggacga gatcttcggc accgtgttta ccgtcgagct gccgcgcacg gcgcaaagct gggtgagcta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a