BuphA.00863.a

PAS/PAC sensor signal transduction histidine kinase

CENTER ID: BuphA.00863.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00863.a.A1.GE32283 Full length( BuphA.00863.a ) 1 502
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1214
RefSeq: YP_001857378.1
UniProt: B2JH94

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEPYRQLSSL THELFRQVVE AAPNAMVMAD REGLVVLVNV QAEKLFGYPR NELVGNSVDM LVPERMRRAH PEFRRMFYGD PRSRPMGTGR DLFALRKDGT EVPVEIGLNP VTTTEGIFVL AAIVDITERR RLEERFRQVV EAAPNAMVMV GQDGKIVLVN AQTENLFGYN REQLFGKSIE TLVPERFRRG HEVSRNAYLH DLKPRPMGTG RDLYGRRRDG SEFPVEIGLN PVRTSNENFV LAAIVDITER KRAEHELMRR TEELARSNQD LEQFAYVASH DLQEPLRAVA GPLQLLQRRY QGQLDARAEE FITHAVDGAT RMQKLIDDLL SYSRVGRSGA VQQLTACAAA LDQALKSLSV VLQESGAQIT RDALPCVMAI PTQLALLFQN LVGNAVKFRQ VDRPVRVDIG ARREGDSWVI SVKDNGIGID PQYFDRIFMI FQRLHSRADY PGTGIGLALC KRIVEQHGGS IWIESQPGAG TTVFFTLKAE DREARIPPQH TP
NT Sequence
ATGGAACCGT ACAGGCAACT GAGCAGCTTG ACTCACGAGC TTTTCCGCCA GGTCGTCGAA GCCGCGCCCA ACGCGATGGT GATGGCGGAT CGCGAAGGTC TCGTCGTGCT CGTGAACGTG CAGGCGGAAA AGCTCTTCGG CTATCCACGC AACGAACTGG TCGGAAATAG TGTCGATATG CTCGTCCCCG AGCGCATGCG CCGCGCGCAT CCCGAGTTCC GTCGCATGTT CTACGGCGAC CCGAGAAGCA GACCGATGGG GACGGGCCGT GATCTGTTTG CGCTACGAAA GGACGGTACC GAAGTTCCCG TCGAGATCGG CCTGAACCCT GTTACGACGA CGGAGGGCAT CTTCGTACTT GCAGCTATCG TCGACATCAC AGAACGCAGA CGCCTGGAGG AGCGCTTCCG GCAAGTGGTG GAAGCTGCGC CGAATGCGAT GGTAATGGTC GGGCAGGACG GAAAGATCGT TCTCGTGAAT GCGCAGACCG AGAACCTGTT CGGCTACAAC CGCGAGCAGC TCTTCGGGAA GTCGATCGAA ACACTCGTGC CGGAACGCTT TCGGAGAGGT CATGAGGTTT CGCGGAACGC CTATCTACAT GATCTGAAAC CGCGCCCGAT GGGCACGGGT CGCGATCTGT ACGGCCGTCG CCGGGATGGC TCGGAGTTTC CCGTGGAGAT CGGGCTCAAC CCCGTCAGAA CCTCCAATGA AAATTTCGTG CTCGCCGCCA TAGTCGACAT TACGGAGCGA AAGCGCGCGG AACACGAACT CATGCGCCGG ACAGAAGAGC TTGCGCGCTC AAACCAGGAT CTCGAACAGT TTGCGTACGT GGCGTCGCAC GATCTACAGG AACCGCTACG CGCCGTCGCG GGGCCCCTTC AGCTGCTTCA GCGCCGCTAT CAAGGACAAC TCGATGCGCG CGCGGAGGAG TTCATCACGC ATGCCGTAGA TGGCGCCACG CGAATGCAAA AGCTGATCGA CGACCTGCTC TCCTATTCCC GTGTCGGACG GTCGGGCGCG GTTCAGCAGT TGACTGCCTG TGCGGCCGCA CTTGATCAGG CACTGAAGAG CTTGAGCGTC GTGCTGCAGG AGTCCGGCGC GCAGATCACT CGGGATGCAC TGCCTTGCGT CATGGCCATT CCGACGCAAC TGGCGCTACT GTTTCAGAAT CTGGTCGGCA ATGCCGTCAA GTTTCGACAG GTCGATCGGC CTGTGCGCGT CGACATCGGT GCGCGCCGTG AGGGCGATAG TTGGGTCATT TCGGTAAAGG ACAATGGTAT CGGCATCGAC CCGCAATACT TCGACCGCAT ATTCATGATC TTTCAGCGCC TTCATTCCCG GGCCGATTAC CCAGGAACGG GCATCGGGCT CGCGCTATGC AAGCGTATCG TCGAGCAGCA TGGAGGAAGC ATCTGGATCG AGTCCCAACC GGGCGCCGGA ACGACGGTCT TCTTTACCTT GAAGGCGGAA GATCGTGAAG CCCGTATCCC ACCACAGCAT ACCCCG
Details for BuphA.00863.a.A1.GE32283
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 58kDa
OBSERVED MW: 58kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 73
PERCENT COVERAGE: 77
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMEPYRQLSS LTHELFRQVV EAAPNAMVMA DREGLVVLVN VQAEKLFGYP RNELVGNSVD MLVPERMRRA HPEFRRMFYG DPRSRPMGTG RDLFALRKDG TEVPVEIGLN PVTTTEGIFV LAAIVDITER RRLEERFRQV VEAAPNAMVM VGQDGKIVLV NAQTENLFGY NREQLFGKSI ETLVPERFRR GHEVSRNAYL HDLKPRPMGT GRDLYGRRRD GSEFPVEIGL NPVRTSNENF VLAAIVDITE RKRAEHELMR RTEELARSNX XSNSLRTWRR TIYRNRYAPS RGPFSCFSAA XKDXSMXAXX XFITXXXXXX XXNAKAXRRX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
attttgttta ctttaagnng gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggaac cgtacaggca actgagcagc ttgactcacg agcttttccg ccaggtcgtc gaagccgcgc ccaacgcgat ggtgatggcg gatcgcgaag gtctcgtcgt gctcgtgaac gtgcaggcgg aaaagctctt cggctatcca cgcaacgaac tggtcggaaa tagtgtcgat atgctcgtcc ccgagcgcat gcgccgcgcg catcccgagt tccgtcgcat gttctacggc gacccgagaa gcagaccgat ggggacgggc cgtgatctgt ttgcgctacg aaaggacggt accgaagttc ccgtcgagat cggcctgaac cctgttacga cgacggaggg catcttcgta cttgcagcta tcgtcgacat cacagaacgc agacgcctgg aggagcgctt ccggcaagtg gtggaagctg cgccgaatgc gatggtaatg gtcgggcagg acggaaagat cgttctcgtg aatgcgcaga ccgagaacct gttcggctac aaccgcgagc agctcttcgg gaagtcgatc gaaacactcg tgccggaacg ctttcggaga ggtcatgagg tttcgcggaa cgcctatcta catgatctga aaccgcgccc gatgggcacg ggtcgcgatc tgtacggccg tcgccgggat ggctcggagt ttcccgtgga gatcgggctc aaccccgtca gaacctccaa tgaaaatttc gtgctcgccg ccatagtcga cattacggag cgaaagcgcg cggaacacga actcatgcgc cggacagaag agcttgcgcg ctcaaaccnn ntctcgaaca gtttgcgtac gtggcgtcgc acgatctaca ggaaccgcta cgcgccgtcg cggggcccct tcagctgctt cagcgccgct ntcaaggacn actcgatgcn cgcgnnggan nanttcatca cgcangncgn annngnnnnc nnnnnnaatg cnaaagctnn ncgacganct gntnnnncnn nntncncnng ntnnnannnn ngggnnnngn nnnnnnnnnn ngnnnnnnnn nnnnnnnnnn cgnnnnnnnn nnnnnnnnnn nnnnnnnngn nnnnnnnnna nnnanntnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncnn nnnnnnnng
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMEPYRQLSS LTHELFRQVV EAAPNAMVMA DREGLVVLVN VQAEKLFGYP RNELVGNSVD MLVPERMRRA HPEFRRMFYG DPRSRPMGTG RDLFALRKDG TEVPVEIGLN PVTTTEGIFV LAAIVDITER RRLEERFRQV VEAAPNAMVM VGQDGKIVLV NAQTENLFGY NREQLFGKSI ETLVPERFRR GHEVSRNAYL HDLKPRPMGT GRDLYGRRRD GSEFPVEIGL NPVRTSNENF VLAAIVDITE RKRAEHELMR RTEELARSNQ DLEQFAYVAS HDLQEPLRAV AGPLQLLQRR YQGQLDARAE EFITHAVDGA TRMQKLIDDL LSYSRVGRSG AVQQLTACAA ALDQALKSLS VVLQESGAQI TRDALPCVMA IPTQLALLFQ NLVGNAVKFR QVDRPVRVDI GARREGDSWV ISVKDNGIGI DPQYFDRIFM IFQRLHSRAD YPGTGIGLAL CKRIVEQHGG SIWIESQPGA GTTVFFTLKA EDREARIPPQ HTP
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggaaccgtac aggcaactga gcagcttgac tcacgagctt ttccgccagg tcgtcgaagc cgcgcccaac gcgatggtga tggcggatcg cgaaggtctc gtcgtgctcg tgaacgtgca ggcggaaaag ctcttcggct atccacgcaa cgaactggtc ggaaatagtg tcgatatgct cgtccccgag cgcatgcgcc gcgcgcatcc cgagttccgt cgcatgttct acggcgaccc gagaagcaga ccgatgggga cgggccgtga tctgtttgcg ctacgaaagg acggtaccga agttcccgtc gagatcggcc tgaaccctgt tacgacgacg gagggcatct tcgtacttgc agctatcgtc gacatcacag aacgcagacg cctggaggag cgcttccggc aagtggtgga agctgcgccg aatgcgatgg taatggtcgg gcaggacgga aagatcgttc tcgtgaatgc gcagaccgag aacctgttcg gctacaaccg cgagcagctc ttcgggaagt cgatcgaaac actcgtgccg gaacgctttc ggagaggtca tgaggtttcg cggaacgcct atctacatga tctgaaaccg cgcccgatgg gcacgggtcg cgatctgtac ggccgtcgcc gggatggctc ggagtttccc gtggagatcg ggctcaaccc cgtcagaacc tccaatgaaa atttcgtgct cgccgccata gtcgacatta cggagcgaaa gcgcgcggaa cacgaactca tgcgccggac agaagagctt gcgcgctcaa accaggatct cgaacagttt gcgtacgtgg cgtcgcacga tctacaggaa ccgctacgcg ccgtcgcggg gccccttcag ctgcttcagc gccgctatca aggacaactc gatgcgcgcg cggaggagtt catcacgcat gccgtagatg gcgccacgcg aatgcaaaag ctgatcgacg acctgctctc ctattcccgt gtcggacggt cgggcgcggt tcagcagttg actgcctgtg cggccgcact tgatcaggca ctgaagagct tgagcgtcgt gctgcaggag tccggcgcgc agatcactcg ggatgcactg ccttgcgtca tggccattcc gacgcaactg gcgctactgt ttcagaatct ggtcggcaat gccgtcaagt ttcgacaggt cgatcggcct gtgcgcgtcg acatcggtgc gcgccgtgag ggcgatagtt gggtcatttc ggtaaaggac aatggtatcg gcatcgaccc gcaatacttc gaccgcatat tcatgatctt tcagcgcctt cattcccggg ccgattaccc aggaacgggc atcgggctcg cgctatgcaa gcgtatcgtc gagcagcatg gaggaagcat ctggatcgag tcccaaccgg gcgccggaac gacggtcttc tttaccttga aggcggaaga tcgtgaagcc cgtatcccac cacagcatac cccgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa