BuphA.00169.a

Replicative DNA helicase

CENTER ID: BuphA.00169.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00169.a.A1.GE33038 Full length( BuphA.00169.a ) 1 462
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.961
RefSeq: YP_001857150.1
UniProt: B2JG22

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAPSKDPQI ESLKVPPHSI EAEQSVLGGL LLDNGAWDRI ADFLAQSDFY RFDHRVIFEH IGRLIAATRP ADVVTVYEAL TTSGKADDVG GLAYLNALAQ NTPSAANIRR YAEIVRDRAV LRRLVSVADE ISADAFNPQG KEVRQLLDEA ESKVFSIAED GARGTQGFLE IGPLLTQVVE RIDTLYHTAN PSDVTGTPTG FVDLDRMTSG MHGGELIIVA GRPSMGKTAF SMNIGEYVAV EYGLPVAVFS MEMPGTQLIM RMLGSVGRLD QHRMRTGRLT DEDWPKLTHA VQKMSEAQIF IDETGGLNPM ELRSRARRLS RQCGKLGLII IDYLQLMSGS SSGENRATEI SEISRSLKSL AKELDVPVIA LSQLNRGLEQ RPNKRPVMSD LRESGAIEQD ADVILFIYRD EVYNPDSPDK GTAEIIIGKQ RNGPIGPVRL TFHGQFTKFD NFAGPQNFYG GE
NT Sequence
ATGAACGCAC CGTCGAAAGA TCCTCAAATC GAGTCGCTGA AAGTCCCGCC CCATTCCATC GAGGCCGAGC AATCGGTGCT GGGCGGTCTG CTGCTCGACA ACGGCGCATG GGACCGCATT GCTGACTTCC TCGCGCAAAG CGACTTCTAC CGCTTCGATC ACCGTGTCAT CTTCGAGCAC ATCGGCAGGC TGATCGCGGC GACGCGTCCC GCCGACGTCG TGACCGTCTA CGAAGCGCTG ACCACCTCGG GCAAGGCCGA CGACGTCGGC GGGCTTGCGT ATCTGAACGC GCTTGCGCAG AACACGCCCA GCGCGGCGAA CATCCGGCGC TATGCGGAAA TCGTTCGCGA CCGCGCGGTG TTGCGCCGCC TCGTGTCCGT GGCCGATGAG ATCTCCGCCG ACGCTTTCAA TCCGCAAGGC AAGGAAGTCC GTCAGCTGCT CGACGAGGCC GAATCGAAGG TGTTCTCGAT CGCGGAAGAC GGCGCGCGCG GCACCCAGGG CTTCCTGGAA ATCGGGCCGC TGCTGACACA GGTCGTCGAG CGCATCGACA CGCTGTACCA CACAGCGAAC CCGAGCGACG TGACGGGCAC GCCGACAGGT TTCGTCGATC TGGACCGCAT GACGTCGGGC ATGCACGGCG GCGAGTTGAT TATCGTCGCG GGGCGTCCTT CGATGGGCAA GACGGCGTTC TCGATGAATA TCGGCGAATA TGTCGCCGTC GAGTATGGCT TGCCCGTCGC GGTGTTCTCG ATGGAAATGC CGGGCACGCA GCTGATCATG CGTATGCTCG GCTCCGTCGG CCGGCTCGAT CAGCACCGCA TGCGAACCGG GCGTCTGACC GACGAGGACT GGCCGAAGCT CACGCATGCC GTGCAGAAAA TGAGCGAAGC GCAGATTTTC ATCGACGAAA CGGGCGGCCT GAACCCGATG GAACTGCGCT CGCGTGCGCG CCGTCTGTCG CGGCAGTGCG GCAAGCTCGG ACTCATCATC ATCGACTACT TGCAGCTGAT GAGCGGTTCG TCGTCAGGCG AAAACCGCGC GACGGAAATT TCGGAAATCT CGCGTTCGCT GAAAAGCCTG GCCAAAGAAC TCGACGTGCC CGTGATCGCG CTGTCGCAGC TGAATCGCGG TCTCGAACAG CGTCCGAACA AGCGTCCCGT GATGTCGGAT TTGCGCGAAT CGGGTGCAAT CGAACAGGAC GCGGACGTGA TCCTGTTCAT TTACCGCGAC GAAGTCTATA ACCCCGACAG CCCCGATAAG GGCACTGCCG AAATCATCAT CGGCAAGCAG CGTAATGGCC CTATCGGCCC CGTTAGACTC ACGTTCCACG GTCAATTCAC GAAGTTCGAC AACTTTGCGG GTCCGCAGAA TTTTTACGGC GGGGAG
Details for BuphA.00169.a.A1.GE33038
HARVESTED ON: 8/16/2011
SEQUENCED ON: 8/22/2011
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 65
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNAPSKDPQ IESLKVPPHS IEAEQSVLGG LLLDNGAWDR IADFLAQSDF YRFDHRVIFE HIGRLIAATR PADVVTVYEA LTTSGKADDV GGLAYLNALA QNTPSAANIR RYAEIVRDRA VLRRLVSVAD EISADAFNPQ GKEVRQLLDE AESKVFSIAE DGARGTQGFL EIGPLLTQVV ERIDTLYHTA NPSDVTGTPT GFVDLDRMTS GMHGGELIIV AGRPSMGKTA FSMNIGEYVA VEYGLXVAVF SMEMXGTQLI MRMLGSVGRL DQHRMRTGRL TDEXWPKLTH AVQK
Validated NT Sequence
ttnnttnnct ttaagaagga gatataccat ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatgaacgca ccgtcgaaag atcctcaaat cgagtcgctg aaagtcccgc cccattccat cgaggccgag caatcggtgc tgggcggtct gctgctcgac aacggcgcat gggaccgcat tgctgacttc ctcgcgcaaa gcgacttcta ccgcttcgat caccgtgtca tcttcgagca catcggcagg ctgatcgcgg cgacgcgtcc cgccgacgtc gtgaccgtct acgaagcgct gaccacctcg ggcaaggccg acgacgtcgg cgggcttgcg tatctgaacg cgcttgcgca gaacacgccc agcgcggcga acatccggcg ctatgcggaa atcgttcgcg accgcgcggt gttgcgccgc ctcgtgtccg tggccgatga gatctccgcc gacgctttca atccgcaagg caaggaagtc cgtcagctgc tcgacgaggc cgaatcgaag gtgttctcga tcgcggaaga cggcgcgcgc ggcacccagg gcttcctgga aatcgggccg ctgctgacac aggtcgtcga gcgcatcgac acgctgtacc acacagcgaa cccgagcgac gtgacgggca cgccgacagg tttcgtcgat ctggaccgca tgacgtcggg catgcacggc ggcgagttga ttatcgtcgc ggggcgtcct tcgatgggca agacggcgtt ctcgatgaat atcggcgaat atgtcgccgt cgagtatggc ttgnccgtcg cggtgttctc gatggaaatg ncgggcacgc agctgatcat gcgtatgctc ggctccgtcg gccggctcga tcagcaccgc atgcgaaccg ggcgtctgac cgacgaggan tggccgaagc tcacgcatgc cgtgcagaaa tgagcgaagc gcanattttc atcgacgaan cgggcggcct gaacccnatg gaactgcgct cgcgtgngcg ccgtctgtcg nngnagtgcn gnagctcngn ctcntcatcn tcgactactt gcagctgatg annngnnngn cntcnggnga ancnnnnnnn nannttcnna antnnnnnnn nnntnaannn nnnnannann nnacnnnnnn nnnnnnnnnc tnnncnnnnn annnnnnnnn nnannnnnnc naannnnnnn nnnnnnnnnn nnnnnnnnng gnnnannnnn nnnnnnnnnn ngnnnnnncn nnnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNAPSKDPQ IESLKVPPHS IEAEQSVLGG LLLDNGAWDR IADFLAQSDF YRFDHRVIFE HIGRLIAATR PADVVTVYEA LTTSGKADDV GGLAYLNALA QNTPSAANIR RYAEIVRDRA VLRRLVSVAD EISADAFNPQ GKEVRQLLDE AESKVFSIAE DGARGTQGFL EIGPLLTQVV ERIDTLYHTA NPSDVTGTPT GFVDLDRMTS GMHGGELIIV AGRPSMGKTA FSMNIGEYVA VEYGLPVAVF SMEMPGTQLI MRMLGSVGRL DQHRMRTGRL TDEDWPKLTH AVQKMSEAQI FIDETGGLNP MELRSRARRL SRQCGKLGLI IIDYLQLMSG SSSGENRATE ISEISRSLKS LAKELDVPVI ALSQLNRGLE QRPNKRPVMS DLRESGAIEQ DADVILFIYR DEVYNPDSPD KGTAEIIIGK QRNGPIGPVR LTFHGQFTKF DNFAGPQNFY GGE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgcaccg tcgaaagatc ctcaaatcga gtcgctgaaa gtcccgcccc attccatcga ggccgagcaa tcggtgctgg gcggtctgct gctcgacaac ggcgcatggg accgcattgc tgacttcctc gcgcaaagcg acttctaccg cttcgatcac cgtgtcatct tcgagcacat cggcaggctg atcgcggcga cgcgtcccgc cgacgtcgtg accgtctacg aagcgctgac cacctcgggc aaggccgacg acgtcggcgg gcttgcgtat ctgaacgcgc ttgcgcagaa cacgcccagc gcggcgaaca tccggcgcta tgcggaaatc gttcgcgacc gcgcggtgtt gcgccgcctc gtgtccgtgg ccgatgagat ctccgccgac gctttcaatc cgcaaggcaa ggaagtccgt cagctgctcg acgaggccga atcgaaggtg ttctcgatcg cggaagacgg cgcgcgcggc acccagggct tcctggaaat cgggccgctg ctgacacagg tcgtcgagcg catcgacacg ctgtaccaca cagcgaaccc gagcgacgtg acgggcacgc cgacaggttt cgtcgatctg gaccgcatga cgtcgggcat gcacggcggc gagttgatta tcgtcgcggg gcgtccttcg atgggcaaga cggcgttctc gatgaatatc ggcgaatatg tcgccgtcga gtatggcttg cccgtcgcgg tgttctcgat ggaaatgccg ggcacgcagc tgatcatgcg tatgctcggc tccgtcggcc ggctcgatca gcaccgcatg cgaaccgggc gtctgaccga cgaggactgg ccgaagctca cgcatgccgt gcagaaaatg agcgaagcgc agattttcat cgacgaaacg ggcggcctga acccgatgga actgcgctcg cgtgcgcgcc gtctgtcgcg gcagtgcggc aagctcggac tcatcatcat cgactacttg cagctgatga gcggttcgtc gtcaggcgaa aaccgcgcga cggaaatttc ggaaatctcg cgttcgctga aaagcctggc caaagaactc gacgtgcccg tgatcgcgct gtcgcagctg aatcgcggtc tcgaacagcg tccgaacaag cgtcccgtga tgtcggattt gcgcgaatcg ggtgcaatcg aacaggacgc ggacgtgatc ctgttcattt accgcgacga agtctataac cccgacagcc ccgataaggg cactgccgaa atcatcatcg gcaagcagcg taatggccct atcggccccg ttagactcac gttccacggt caattcacga agttcgacaa ctttgcgggt ccgcagaatt tttacggcgg ggagtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa