SSGCID: Target Page

BuphA.00161.a

Adenosine deaminase

CENTER ID:	BuphA.00161.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00161.a.B1.GE40284	Full length( BuphA.00161.a )		1	346

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.750
RefSeq:	YP_001856945.1
UniProt:	B2JEX0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTTTTLSPLA RKTSLAPKAE LHIHIEGSLE PELIFKLAER NGVKLAYESI DALRAAYAFT DLQSFLDIYY AGASVLLKEE DFYDMTMAYV ERALADNVVH TEIFFDPQTH TERGVPIATV VAGIERALAE AQTRGLTSKL ILCFLRHLSE DDALATFDAA LPLFDQYRHR LIGVGLDSSE HGHPPSKFER VFAKARAKGL KLVAHAGEEG PPSYIYEALD LLKVDRVDHG VRSIEDPALV TRLADSRVAL TVCPLSNLKL CVFDDLTRHT LKALLDAGVA VTVNSDDPAY FGGYVNENYL ATIDALKLDD AEVYTIIRNG FEASFVTPAQ RDAMIAKLDA HWRASA

NT Sequence

ATGACGACAA CCACACTGTC CCCGCTAGCT CGCAAAACCA GCCTCGCACC GAAGGCCGAG TTGCACATTC ATATCGAAGG TTCGCTGGAA CCCGAGCTGA TCTTCAAGCT TGCCGAACGC AACGGCGTGA AACTCGCGTA CGAATCGATC GATGCGCTGC GTGCCGCGTA TGCGTTCACC GATCTGCAGT CGTTCCTCGA CATCTACTAC GCGGGCGCCA GCGTGCTGCT GAAGGAAGAG GACTTCTACG ACATGACGAT GGCTTACGTC GAGCGCGCGC TCGCGGACAA CGTCGTGCAT ACGGAAATCT TCTTCGATCC GCAGACGCAT ACGGAGCGCG GCGTGCCGAT TGCGACCGTG GTCGCGGGCA TCGAGCGCGC ACTTGCCGAA GCGCAAACGC GCGGTCTCAC GAGCAAACTG ATCTTGTGTT TCCTGCGGCA TCTGTCCGAA GACGATGCAC TCGCGACGTT CGACGCGGCG CTGCCGCTGT TCGATCAGTA CAGGCACCGG CTGATCGGTG TCGGGCTCGA TTCGTCGGAG CACGGCCATC CGCCGTCCAA GTTCGAGCGC GTGTTCGCGA AGGCACGCGC CAAAGGTCTG AAGCTCGTCG CACATGCCGG CGAAGAAGGC CCGCCGTCGT ACATCTACGA AGCGCTCGAT CTGCTGAAGG TCGACCGCGT CGACCACGGC GTGCGCAGCA TCGAAGACCC GGCGCTCGTC ACGCGCCTTG CCGATTCGCG TGTCGCTCTG ACGGTCTGCC CGCTGTCGAA CCTGAAGCTG TGCGTGTTCG ACGATCTGAC GAGGCATACG CTGAAGGCGC TGCTCGACGC GGGCGTCGCC GTCACCGTGA ACTCCGACGA CCCCGCGTAC TTCGGCGGCT ACGTCAACGA AAACTACCTC GCGACGATCG ACGCCCTCAA GCTCGACGAC GCCGAGGTGT ACACGATCAT CCGCAACGGC TTCGAGGCGT CGTTCGTCAC GCCCGCGCAG CGCGATGCGA TGATCGCGAA ACTCGATGCG CACTGGCGCG CATCTGCC

Details for BuphA.00161.a.B1.GE40284

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	39kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT TTTLSPLARK TSLAPKAELH IHIEGSLEPE LIFKLAERNG VKLAYESIDA LRAAYAFTDL QSFLDIYYAG ASVLLKEEDF YDMTMAYVER ALADNVVHTE IFFDPQTHTE RGVPIATVVA GIERALAEAQ TRGLTSKLIL CFLRHLSEDD ALATFDAALP LFDQYRHRLI GVGLDSSEHG HPPSKFERVF AKARAKGLKL VAHAGEEGPP SYIYEALDLL KVDRVDHGVR SIEDPALVTR LADSRVALTV CPLSNLKLCV FDDLTRHTLK ALLDAGVAVT VNSDDPAYFG GYVNENYLAT IDALKLDDAE VYTIIRNGFE ASFVTPAQRD AMIAKL

Validated NT Sequence

cgagtttcgc gatcatcgca tcgcgctgcg cgggcgtgac gaacgacgcc tcgaagccgt tgcggatgat cgtgtacacc tcggcgtcgt cgagcttgag ggcgtcgatc gtcgcgaggt agttttcgtt gacgtagccg ccgaagtacg cggggtcgtc ggagttcacg gtgacggcga cgcccgcgtc gagcagcgcc ttcagcgtat gcctcgtcag atcgtcgaac acgcacagct tcaggttcga cagcgggcag accgtcagag cgacacgcga atcggcaagg cgcgtgacga gcgccgggtc ttcgatgctg cgcacgccgt ggtcgacgcg gtcgaccttc agcagatcga gcgcttcgta gatgtacgac ggcgggcctt cttcgccggc atgtgcgacg agcttcagac ctttggcgcg tgccttcgcg aacacgcgct cgaacttgga cggcggatgg ccgtgctccg acgaatcgag cccgacaccg atcagccggt gcctgtactg atcgaacagc ggcagcgccg cgtcgaacgt cgcgagtgca tcgtcttcgg acagatgccg caggaaacac aagatcagtt tgctcgtgag accgcgcgtt tgcgcttcgg caagtgcgcg ctcgatgccc gcgaccacgg tcgcaatcgg cacgccgcgc tccgtatgcg tctgcggatc gaagaagatt tccgtatgca cgacgttgtc cgcgagcgcg cgctcgacgt aagccatcgt catgtcgtag aagtcctctt ccttcagcag cacgctggcg cccgcgtagt agatgtcgag gaacgactgc agatcggtga acgcatacgc ggcacgcagc gcatcgatcg attcgtacgc gagtttcacg ccgttgcgtt cggcaagctt gaagatcagc tcgggttcca gcgaaccttc gatatgaatg tgcaactcgg ccttcggtgc gaggctggtt ttgcgagcta gcggggacag tgtggttgtc gtcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga caaccacact gtccccgcta gctcgcaaaa ccagcctcgc accgaaggcc gagttgcaca ttcatatcga aggttcgctg gaacccgagc tgatcttcaa gcttgccgaa cgcaacggcg tgaaactcgc gtacgaatcg atcgatgcgc tgcgtgccgc gtatgcgttc accgatctgc agtcgttcct cgacatctac tacgcgggcg ccagcgtgct gctgaaggaa gaggacttct acgacatgac gatggcttac gtcgagcgcg cgctcgcgga caacgtcgtg catacggaaa tcttcttcga tccgcagacg catacggagc gcggcgtgcc gattgcgacc gtggtcgcgg gcatcgagcg cgcacttgcc gaagcgcaaa cgcgcggtct cacgagcaaa ctgatcttgt gtttcctgcg gcatctgtcc gaagacgatg cactcgcgac gttcgacgcg gcgctgccgc tgttcgatca gtacaggcac cggctgatcg gtgtcgggct cgattcgtcg gagcacggcc atccgccgtc caagttcgag cgcgtgttcg cgaaggcacg cgccaaaggt ctgaagctcg tcgcacatgc cggcgaagaa ggcccgccgt cgtacatcta cgaagcgctc gatctgctga aggtcgaccg cgtcgaccac ggcgtgcgca gcatcgaaga cccggcgctc gtcacgcgcc ttgccgattc gcgtgtcgct ctgacggtct gcccgctgtc gaacctgaag ctgtgcgtgt tcgacgatct gacgaggcat acgctgaagg cgctgctcga cgcgggcgtc gccgtcaccg tgaactccga cgaccccgcg tacttcggcg gctacgtcaa cgaaaactac ctcgcgacga tcgacgccct caagctcgac gacgccgagg tgtacacgat catccgcaac ggcttcgagg cgtcgttcgt cacgcccgcg cagcgcgatg cgatgatcgc gaaactcgat gcgcactggc gcgcatctgc ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat