SSGCID: Target Page

BuphA.00158.a

Glutamate racemase

CENTER ID:	BuphA.00158.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00158.a.B1.GE40283	Full length( BuphA.00158.a )		1	303

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.2072
RefSeq:	YP_001858168.1
UniProt:	B2JD66

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MASVETMTAS PPISTSSTSL PALVSLDPRA PVGIFDSGLG GLSVLRAVRS QLPSEAIVYV ADSLYAPYGE RDDDFIADRT LAIGEWLIAQ GAKALVVACN TATAQSIALV REKLPIQLIG VEPGVKPAAQ QSKSRVAGVL ATRVTLRSAR FQGLLERYAS DCRFLCQPGH GLVEAVERCD IESAELRALL VSYVQPMLDA GADTLVLGCT HYPFLDAAIR DIAGDRLTLI DTSVAIARQL ERVLDQQGLR SPERDTVPPP RFCSTSDGAH LKQLAKTLLN IDAPVERVHI PSRRTIARDP HAA

NT Sequence

ATGGCTTCCG TTGAAACCAT GACCGCTTCG CCCCCCATTT CCACCTCGTC CACTTCATTG CCGGCGCTTG TCTCGCTCGA TCCGCGCGCG CCTGTCGGCA TTTTCGATTC GGGTTTGGGC GGGTTGTCGG TGCTGCGCGC GGTTCGCTCG CAACTGCCGA GTGAAGCGAT TGTCTACGTC GCGGATTCGC TCTACGCGCC CTATGGTGAG CGCGACGACG ATTTCATCGC CGACCGCACG CTCGCGATCG GCGAATGGCT CATCGCGCAA GGCGCGAAGG CGCTCGTAGT CGCGTGCAAT ACGGCGACCG CGCAGTCGAT CGCGCTGGTG CGAGAAAAAC TGCCCATTCA ATTGATCGGC GTCGAACCGG GTGTAAAGCC CGCGGCGCAG CAGTCGAAAT CGCGTGTCGC AGGCGTGCTC GCGACCCGCG TCACGCTGCG CAGCGCGCGC TTCCAAGGCC TGCTTGAACG TTACGCCAGC GACTGCCGCT TCCTGTGCCA GCCAGGACAC GGCCTCGTGG AAGCTGTGGA GCGCTGCGAC ATCGAATCGG CCGAACTGCG CGCGCTGCTC GTCAGCTACG TCCAGCCAAT GCTCGACGCG GGCGCCGACA CGCTCGTGCT CGGCTGCACG CATTACCCGT TTCTGGACGC AGCGATCCGC GACATCGCCG GTGACCGGCT GACGCTGATC GACACGAGCG TCGCGATCGC CCGGCAACTG GAACGCGTGC TCGACCAGCA AGGGCTGCGC TCGCCGGAGC GCGATACCGT GCCGCCGCCG CGCTTTTGCT CGACCAGCGA CGGCGCACAT CTGAAGCAGC TCGCCAAAAC ATTGCTGAAT ATCGACGCGC CTGTCGAGCG CGTACATATC CCTTCGCGCC GCACGATCGC ACGAGATCCC CACGCCGCC

Details for BuphA.00158.a.B1.GE40283

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	97
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA SVETMTASPP ISTSSTSLPA LVSLDPRAPV GIFDSGLGGL SVLRAVRSQL PSEAIVYVAD SLYAPYGERD DDFIADRTLA IGEWLIAQGA KALVVACNTA TAQSIALVRE KLPIQLIGVE PGVKPAAQQS KSRVAGVLAT RVTLRSARFQ GLLERYASDC RFLCQPGHGL VEAVERCDIE SAELRALLVS YVQPMLDAGA DTLVLGCTHY PFLDAAIRDI AGDRLTLIDT SVAIARQLER VLDQQGLRSP ERDTVPPPRF CSTSDGAHLK QLAKTLLNID APVERVHIPS RRTXXXXSPR RL

Validated NT Sequence

acttaggcgg cgtggggatc nnnnnnnnnt cgtgcggcgc gaagggatat gtacgcgctc gacaggcgcg tcgatattca gcaatgtttt ggcgagctgc ttcagatgtg cgccgtcgct ggtcgagcaa aagcgcggcg gcggcacggt atcgcgctcc ggcgagcgca gcccttgctg gtcgagcacg cgttccagtt gccgggcgat cgcgacgctc gtgtcgatca gcgtcagccg gtcaccggcg atgtcgcgga tcgctgcgtc cagaaacggg taatgcgtgc agccgagcac gagcgtgtcg gcgcccgcgt cgagcattgg ctggacgtag ctgacgagca gcgcgcgcag ttcggccgat tcgatgtcgc agcgctccac agcttccacg aggccgtgtc ctggctggca caggaagcgg cagtcgctgg cgtaacgttc aagcaggcct tggaagcgcg cgctgcgcag cgtgacgcgg gtcgcgagca cgcctgcgac acgcgatttc gactgctgcg ccgcgggctt tacacccggt tcgacgccga tcaattgaat gggcagtttt tctcgcacca gcgcgatcga ctgcgcggtc gccgtattgc acgcgactac gagcgccttc gcgccttgcg cgatgagcca ttcgccgatc gcgagcgtgc ggtcggcgat gaaatcgtcg tcgcgctcac catagggcgc gtagagcgaa tccgcgacgt agacaatcgc ttcactcggc agttgcgagc gaaccgcgcg cagcaccgac aacccgccca aacccgaatc gaaaatgccg acaggcgcgc gcggatcgag cgagacaagc gccggcaatg aagtggacga ggtggaaatg gggggcgaag cggtcatggt ttcaacggaa gccatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctt ccgttgaaac catgaccgct tcgcccccca tttccacctc gtccacttca ttgccggcgc ttgtctcgct cgatccgcgc gcgcctgtcg gcattttcga ttcgggtttg ggcgggttgt cggtgctgcg cgcggttcgc tcgcaactgc cgagtgaagc gattgtctac gtcgcggatt cgctctacgc gccctatggt gagcgcgacg acgatttcat cgccgaccgc acgctcgcga tcggcgaatg gctcatcgcg caaggcgcga aggcgctcgt agtcgcgtgc aatacggcga ccgcgcagtc gatcgcgctg gtgcgagaaa aactgcccat tcaattgatc ggcgtcgaac cgggtgtaaa gcccgcggcg cagcagtcga aatcgcgtgt cgcaggcgtg ctcgcgaccc gcgtcacgct gcgcagcgcg cgcttccaag gcctgcttga acgttacgcc agcgactgcc gcttcctgtg ccagccagga cacggcctcg tggaagctgt ggagcgctgc gacatcgaat cggccgaact gcgcgcgctg ctcgtcagct acgtccagcc aatgctcgac gcgggcgccg acacgctcgt gctcggctgc acgcattacc cgtttctgga cgcagcgatc cgcgacatcg ccggtgaccg gctgacgctg atcgacacga gcgtcgcgat cgcccggcaa ctggaacgcg tgctcgacca gcaagggctg cgctcgccgg agcgcgatac cgtgccgccg ccgcgctttt gctcgaccag cgacggcgca catctgaagc agctcgccaa aacattgctg aatatcgacg cgcctgtcga gcgcgtacat atcccttcgc gccgcacgat cgcacgagat ccccacgccg cctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat