BuphA.00156.a

Threonyl-tRNA synthetase

CENTER ID: BuphA.00156.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00156.a.B1.GE39145 Full length( BuphA.00156.a ) 1 635
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1665
RefSeq: YP_001857786.1
UniProt: B2JJJ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVSIRLPDGS VRQYEHPVTV AEVAASIGPG LAKAALGGKI DGELVDTSAL IDHDVALAIV TDKDADGLDI IRHSTAHLLA YAVKDLFPEA QVTIGPVIDN GFYYDFSYSR PFTPEDLEKI EKRMQELAKK DEPVSRRVVS RDEAADYFKS IGEKYKAEII ESIPASDDIK LYSHGGFTDL CRGPHVPSTG KLKVFKLMKV AGAYWRGDSK NEQLQRIYGT AWTKKEDQDA YLHMLEEAEK RDHRKLGKQL DLFHMQDESP GMVFWHPRGW TLWQQVEQYM RRRVNEAGYL EIKTPMIMDR SLWEASGHWQ NYRDNMFTTE SEKRDYAIKP MNCPGHVQVF NHGLRSYRDL PLRYAEFGSC HRNESSGALH GLMRVRGFVQ DDAHIFCTED QFISESIAFN TLAMSVYKDF GFDNVEIKLS LRPDARAGTD EIWDRAEQGL RDALTACGVT WEELPGEGAF YGPKVEYHIK DALGRSWQCG TLQLDMVLPE RLGAEFVAED NSRRRPIMLH RAIVGSMERF LGILIEHHAG AMPAWLAPMQ VVVMNIAESQ AEYAQSLAQS LQKQGVRVEA DLRNEKISYK IREHTLEKVP YLLVVGDKER DAQTVAVRAR GGIDLGVMPV DAFSERLRQD VQTFN
NT Sequence
ATGGTTTCGA TACGACTGCC TGATGGTTCT GTTCGACAAT ACGAGCATCC CGTGACGGTC GCCGAAGTGG CGGCCTCGAT CGGCCCCGGT CTCGCGAAGG CGGCGCTCGG CGGCAAGATC GACGGTGAAC TGGTGGACAC GTCCGCGCTG ATCGATCACG ACGTCGCACT TGCGATCGTG ACGGACAAGG ACGCCGACGG CCTCGACATC ATTCGCCATT CGACGGCGCA CCTGCTCGCG TACGCCGTCA AGGATCTGTT TCCCGAAGCG CAGGTAACGA TCGGGCCGGT GATCGACAAC GGCTTCTATT ACGACTTCTC GTACAGCCGT CCCTTCACGC CCGAGGATCT CGAGAAGATC GAAAAGCGCA TGCAGGAGCT CGCAAAGAAG GACGAGCCGG TATCGCGCCG CGTGGTGTCG CGCGACGAGG CCGCCGACTA TTTCAAGAGC ATTGGCGAAA AGTACAAGGC CGAGATCATC GAGTCGATTC CCGCCAGCGA CGACATCAAG CTGTATTCGC ATGGCGGCTT CACCGATCTG TGCCGTGGCC CGCACGTGCC GTCGACGGGC AAGCTGAAGG TCTTCAAGCT GATGAAGGTC GCGGGCGCGT ATTGGCGCGG CGACTCGAAG AACGAGCAGT TGCAGCGTAT TTACGGCACG GCGTGGACGA AGAAAGAAGA CCAGGACGCC TACCTGCACA TGCTCGAAGA GGCGGAAAAG CGCGACCACC GCAAGCTCGG CAAACAACTC GACCTGTTCC ACATGCAGGA CGAGTCGCCG GGCATGGTGT TCTGGCATCC GCGCGGCTGG ACGCTGTGGC AGCAGGTCGA GCAGTACATG CGCCGTCGCG TGAACGAGGC TGGCTACCTC GAAATCAAGA CGCCGATGAT CATGGACCGC TCGCTGTGGG AGGCATCGGG TCACTGGCAG AACTATCGTG ACAACATGTT CACGACGGAG TCGGAAAAGC GCGACTACGC GATCAAGCCG ATGAACTGCC CGGGTCACGT GCAGGTGTTC AATCACGGCC TGCGTTCGTA TCGCGACCTG CCACTGCGTT ATGCCGAGTT CGGTTCTTGC CATCGCAACG AATCGTCAGG CGCGCTGCAT GGTTTGATGC GCGTGCGCGG CTTCGTGCAG GACGATGCGC ACATTTTCTG TACGGAAGAC CAGTTCATCA GCGAGTCGAT CGCGTTCAAC ACGCTGGCGA TGAGCGTCTA CAAGGACTTC GGGTTCGATA ACGTGGAGAT CAAGCTGTCA TTGCGTCCGG ACGCGCGTGC CGGCACGGAC GAAATCTGGG ATCGCGCCGA GCAGGGGCTG CGCGACGCGC TGACGGCCTG TGGCGTGACG TGGGAAGAAT TGCCGGGCGA GGGTGCGTTC TACGGGCCGA AGGTGGAATA CCACATCAAG GATGCGCTCG GCCGTTCATG GCAGTGCGGC ACGCTGCAGC TCGATATGGT GCTGCCGGAG CGCCTCGGCG CCGAATTCGT CGCGGAAGAC AACAGCCGTC GCAGGCCGAT CATGCTGCAT CGTGCAATCG TCGGATCAAT GGAGCGTTTC CTCGGCATTC TGATCGAGCA CCATGCCGGT GCGATGCCTG CGTGGCTGGC GCCGATGCAG GTTGTGGTGA TGAATATCGC GGAAAGTCAG GCGGAATACG CACAATCTCT AGCTCAATCG TTGCAAAAAC AAGGGGTTAG AGTGGAGGCC GATTTGCGCA ACGAGAAGAT TAGCTATAAA ATACGCGAGC ACACGCTCGA AAAGGTCCCG TATCTGCTGG TTGTCGGCGA CAAGGAGCGT GATGCACAAA CGGTAGCCGT GCGTGCCCGT GGCGGCATCG ATCTGGGCGT GATGCCCGTT GATGCCTTCA GCGAGCGTCT GCGCCAGGAT GTGCAGACGT TCAAC
Details for BuphA.00156.a.B1.GE39145
HARVESTED ON: 5/6/2015
SEQUENCED ON: 5/6/2015
EXPECTED MW: 73kDa
OBSERVED MW: 73kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMV SIRLPDGSVR QYEHPVTVAE VAASIGPGLA KAALGGKIDG ELVDTSALID HDVALAIVTD KDADGLDIIR HSTAHLLAYA VKDLFPEAQV TIGPVIDNGF YYDFSYSRPF TPEDLEKIEK RMQELAKKDE PVSRRVVSRD EAADYFKSIG EKYKAEIIES IPASDDIKLY SHGGFTDLCR GPHVPSTGKL KVFKLMKVAG AYWRGDSKNE QLQRIYGTAW TKKEDQDAYL HMLEEAEKRD HRKLGKQLDL FHMQDESPGM VFWHPRGWTL WQQVEQYMRR RVNEAGYLEI KTPMIMDRSL WEASGHWQNY RDNMFTTESE KRDYAIKPMN CPGHVQVFNH GLRSYRDLPL RYAEFGSCHR NESSGALHGL MRVRGFVQDD AHIFCTEDQF ISESIAFNTL AMSVYKDFGF DNVEIKLSLR PDARAGTDEI WDRAEQGLRD ALTACGVTWE ELPGEGAFYG PKVEYHIKDA LGRSWQCGTL QLDMVLPERL GAEFVAEDNS RRRPIMLHRA IVGSMERFLG ILIEHHAGAM PAWLAPMQVV VMNIAESQAE YAQSLAQSLQ KQGVRVEADL RNEKISYKIR EHTLEKVPYL LVVGDKERDA QTVAVRARGG IDLGVMPVDA FSERLRQDVQ TFN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggttt cgatacgact gcctgatggt tctgttcgac aatacgagca tcccgtgacg gtcgccgaag tggcggcctc gatcggcccc ggtctcgcga aggcggcgct cggcggcaag atcgacggtg aactggtgga cacgtccgcg ctgatcgatc acgacgtcgc acttgcgatc gtgacggaca aggacgccga cggcctcgac atcattcgcc attcgacggc gcacctgctc gcgtacgccg tcaaggatct gtttcccgaa gcgcaggtaa cgatcgggcc ggtgatcgac aacggcttct attacgactt ctcgtacagc cgtcccttca cgcccgagga tctcgagaag atcgaaaagc gcatgcagga gctcgcaaag aaggacgagc cggtatcgcg ccgcgtggtg tcgcgcgacg aggccgccga ctatttcaag agcattggcg aaaagtacaa ggccgagatc atcgagtcga ttcccgccag cgacgacatc aagctgtatt cgcatggcgg cttcaccgat ctgtgccgtg gcccgcacgt gccgtcgacg ggcaagctga aggtcttcaa gctgatgaag gtcgcgggcg cgtattggcg cggcgactcg aagaacgagc agttgcagcg tatttacggc acggcgtgga cgaagaaaga agaccaggac gcctacctgc acatgctcga agaggcggaa aagcgcgacc accgcaagct cggcaaacaa ctcgacctgt tccacatgca ggacgagtcg ccgggcatgg tgttctggca tccgcgcggc tggacgctgt ggcagcaggt cgagcagtac atgcgccgtc gcgtgaacga ggctggctac ctcgaaatca agacgccgat gatcatggac cgctcgctgt gggaggcatc gggtcactgg cagaactatc gtgacaacat gttcacgacg gagtcggaaa agcgcgacta cgcgatcaag ccgatgaact gcccgggtca cgtgcaggtg ttcaatcacg gcctgcgttc gtatcgcgac ctgccactgc gttatgccga gttcggttct tgccatcgca acgaatcgtc aggcgcgctg catggtttga tgcgcgtgcg cggcttcgtg caggacgatg cgcacatttt ctgtacggaa gaccagttca tcagcgagtc gatcgcgttc aacacgctgg cgatgagcgt ctacaaggac ttcgggttcg ataacgtgga gatcaagctg tcattgcgtc cggacgcgcg tgccggcacg gacgaaatct gggatcgcgc cgagcagggg ctgcgcgacg cgctgacggc ctgtggcgtg acgtgggaag aattgccggg cgagggtgcg ttctacgggc cgaaggtgga ataccacatc aaggatgcgc tcggccgttc atggcagtgc ggcacgctgc agctcgatat ggtgctgccg gagcgcctcg gcgccgaatt cgtcgcggaa gacaacagcc gtcgcaggcc gatcatgctg catcgtgcaa tcgtcggatc aatggagcgt ttcctcggca ttctgatcga gcaccatgcc ggtgcgatgc ctgcgtggct ggcgccgatg caggttgtgg tgatgaatat cgcggaaagt caggcggaat acgcacaatc tctagctcaa tcgttgcaaa aacaaggggt tagagtggag gccgatttgc gcaacgagaa gattagctat aaaatacgcg agcacacgct cgaaaaggtc ccgtatctgc tggttgtcgg cgacaaggag cgtgatgcac aaacggtagc cgtgcgtgcc cgtggcggca tcgatctggg cgtgatgccc gttgatgcct tcagcgagcg tctgcgccag gatgtgcaga cgttcaactg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat