SSGCID: Target Page

BuphA.00153.a

DNA primase

CENTER ID:	BuphA.00153.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00153.a.B1.GE35969	Full length( BuphA.00153.a )		1	630

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.4158
RefSeq:	YP_001860111.1
UniProt:	B2JP62

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIPHSFLQDL LNRVDIVDVV GKYVQLKKGG ANFMGLCPFH NEKSPSFTVS PTKQFYHCFG CGAHGTAIGF LMEHAGLSFP EAVNDLAQSV GLTVPQEPSP MRGGASGGAA YGAGGEAYAP AVPKAVTTAL SDVMQTASEF YKAQLRGAPN AIQYLKKRGL TGEIAKRFGL GYAPDGWQNL EAAFPNYRDD ALVESGLVIV SEKSDAQGQN RRYDRFRERV MFPIRNVKGQ VIGFGGRVLD GGEPKYLNSP ETPLFNKGSE LYGLFEARLA IREQKYVLVV EGYMDVVALA QLGFENAVAT LGTACTPIHV QKLMRQTDTV VFSFDGDSAG RRAARRALDA CLPHAADNRT IRFLFLPAEH DPDSYVREFG TQAFAEQVRR AMPLSQFLLN EVLAGKELDQ PEGRARALFD AKPLLQMLPA NALRAQIMHM FADRLDVPFE EVAALCEVDA RIAAVARSAP ARKDRRSVTG LEQRALRNLV MHPRIVAVLD EEEERALLTV TRHGELFEEV TTHARGLGDS AEFQLLSDLL RNGANAPTFE EIFREILDYD ENVRDLLLKN PEDATVAEER REHERLAGEE LKAAILKMRY DAYSERLTQL SRQSSLTPEE LKEYQELDRT RADMKRQRGQ

NT Sequence

GTGATTCCGC ATTCCTTCCT GCAAGACCTG CTTAACCGCG TCGATATCGT CGACGTGGTC GGCAAGTACG TGCAGCTGAA AAAGGGCGGC GCGAACTTCA TGGGACTCTG CCCGTTTCAC AACGAAAAGA GCCCTTCGTT CACCGTTAGT CCGACTAAGC AGTTCTATCA TTGTTTCGGC TGTGGCGCGC ATGGCACCGC CATTGGCTTT CTGATGGAGC ACGCGGGGCT GTCGTTTCCC GAAGCCGTCA ACGATCTGGC GCAATCGGTC GGTCTGACCG TGCCGCAGGA GCCTTCGCCG ATGCGCGGCG GCGCTTCGGG CGGCGCCGCT TACGGCGCGG GCGGCGAAGC CTATGCGCCT GCCGTGCCGA AGGCCGTCAC GACGGCGCTG TCCGACGTCA TGCAGACGGC CAGCGAGTTC TACAAGGCGC AGTTGCGCGG CGCGCCGAAC GCGATCCAGT ACCTGAAAAA GCGCGGCCTC ACTGGTGAAA TCGCGAAACG CTTCGGCCTC GGCTACGCGC CCGACGGCTG GCAGAACCTC GAAGCCGCAT TCCCCAACTA TCGGGACGAT GCGCTCGTCG AGTCGGGCCT CGTGATTGTC AGCGAGAAGT CGGACGCGCA GGGTCAGAAC CGCCGCTATG ACCGTTTCCG CGAGCGCGTG ATGTTCCCGA TCCGCAACGT GAAAGGCCAG GTCATCGGCT TCGGCGGACG CGTGCTCGAC GGCGGCGAGC CGAAGTATTT GAATTCTCCC GAAACGCCTT TATTTAACAA AGGCAGCGAA CTGTACGGTC TGTTCGAGGC GCGGCTCGCG ATCCGCGAGC AGAAGTACGT GCTGGTCGTC GAAGGGTATA TGGACGTGGT CGCGCTGGCG CAGTTGGGCT TCGAGAACGC GGTTGCGACG CTCGGCACCG CCTGTACGCC GATTCATGTG CAGAAACTGA TGCGGCAGAC GGACACGGTA GTGTTCAGCT TCGACGGCGA CTCGGCGGGC CGGCGCGCGG CGCGGCGCGC GCTCGACGCG TGCCTGCCGC ATGCCGCCGA CAATCGGACC ATCCGTTTCC TGTTCCTGCC GGCCGAGCAT GATCCCGACA GCTATGTGCG CGAGTTCGGC ACGCAAGCGT TTGCGGAACA GGTGCGCCGC GCGATGCCGC TGTCACAGTT CCTGCTGAAC GAAGTGCTGG CGGGCAAGGA ACTCGACCAG CCCGAAGGCC GCGCACGAGC GCTGTTCGAC GCGAAGCCGC TGCTGCAGAT GCTTCCGGCC AATGCGCTGC GCGCACAGAT CATGCACATG TTCGCGGACC GGCTCGACGT GCCGTTCGAG GAAGTCGCAG CGTTATGCGA AGTCGATGCG CGGATCGCCG CTGTAGCACG CAGCGCGCCT GCTCGAAAAG ACCGGCGCAG CGTGACGGGT CTGGAGCAGC GGGCGCTGCG CAATCTCGTC ATGCACCCGC GCATCGTCGC CGTGCTCGAC GAAGAGGAAG AGCGGGCGCT GCTGACTGTC ACACGGCACG GCGAACTGTT CGAGGAAGTC ACGACGCATG CCCGCGGGCT GGGCGACTCG GCTGAATTCC AGTTGCTGTC CGACCTGTTG CGAAACGGGG CTAATGCCCC CACGTTCGAG GAAATCTTCC GGGAAATTCT GGACTATGAT GAAAACGTCC GGGATCTGCT ACTGAAGAAT CCCGAAGACG CGACGGTTGC CGAAGAGCGA CGTGAGCACG AGCGCCTTGC CGGGGAAGAA TTGAAAGCGG CCATTCTGAA AATGCGCTAT GACGCATACA GCGAACGGCT GACCCAACTG TCGCGCCAGT CGAGCCTCAC ACCGGAGGAA CTGAAGGAGT ATCAGGAACT TGACAGGACG CGTGCCGACA TGAAGCGTCA GCGAGGGCAG

Details for BuphA.00153.a.B1.GE35969

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	71kDa
OBSERVED MW:	71kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	82
PERCENT COVERAGE:	57

Validated AA Sequence

Validated NT Sequence

tttgttnnnt ttaagaagga ganataccat ggctcaccac caccaccacc atatggtgat tccgcattcc ttcctgcaag acctgcttaa ccgcgtcgat atcgtcgacg tggtcggcaa gtacgtgcag ctgaaaaagg gcggcgcgaa cttcatggga ctctgcccgt ttcacaacga aaagagccct tcgttcaccg ttagtccgac taagcagttc tatcattgtt tcggctgtgg cgcgcatggc accgccattg gctttctgat ggagcacgcg gggctgtcgt ttcccgaagc cgtcaacgat ctggcgcaat cggtcggtct gaccgtgccg caggagcctt cgccgatgcg cggcggcgct tcgggcggcg ccgcttacgg cgcgggcggc gaagcctatg cgcctgccgt gccgaaggcc gtcacgacgg cgctgtccga cgtcatgcag acggccagcg agttctacaa ggcgcagttg cgcggcgcgc cgaacgcgat ccagtacctg aaaaagcgcg gcctcactgg tgaaatcgcg aaacgcttcg gcctcggcta cgcgcccgac ggctggcaga acctcgaagc cgcattcccc aactatcggg acgatgcgct cgtcgagtcg ggcctcgtga ttgtcagcga gaagtcggac gcgcagggtc agaaccgccg ctatgaccgt ttccgcgagc gcgtgatgtt cccgatccgc aacgtgaaag gccaggtcat cggcttcggc ggacgcgtgc tcgacggcgg cgagccgaag tatttgaatt ctcccgaaac gcctttattt aacaaaggca gcgaactgta cggtctgttc gaggcgcggc tcgcgatccg cgagcagaag tacgtgctgg tcgtcgaang ggtatatgga cgtggtcgcg ctggcgcatt gggcttcnag aacgcngntt gcgacgctcg gcancnccng nacnccgatt cnngtgcaga aactgatgcn gcaganngna nncggnanng ttcagcttcg acggcnactc ggnngnnngn nnnnggcgnn nnnnngctcn acgnnnngca tncnnncnan nncngnanna tnannnnccn nnngcnnnnn nnnncnnnnn nnnnncc

Expected Protein Sequence

MAHHHHHHMV IPHSFLQDLL NRVDIVDVVG KYVQLKKGGA NFMGLCPFHN EKSPSFTVSP TKQFYHCFGC GAHGTAIGFL MEHAGLSFPE AVNDLAQSVG LTVPQEPSPM RGGASGGAAY GAGGEAYAPA VPKAVTTALS DVMQTASEFY KAQLRGAPNA IQYLKKRGLT GEIAKRFGLG YAPDGWQNLE AAFPNYRDDA LVESGLVIVS EKSDAQGQNR RYDRFRERVM FPIRNVKGQV IGFGGRVLDG GEPKYLNSPE TPLFNKGSEL YGLFEARLAI REQKYVLVVE GYMDVVALAQ LGFENAVATL GTACTPIHVQ KLMRQTDTVV FSFDGDSAGR RAARRALDAC LPHAADNRTI RFLFLPAEHD PDSYVREFGT QAFAEQVRRA MPLSQFLLNE VLAGKELDQP EGRARALFDA KPLLQMLPAN ALRAQIMHMF ADRLDVPFEE VAALCEVDAR IAAVARSAPA RKDRRSVTGL EQRALRNLVM HPRIVAVLDE EEERALLTVT RHGELFEEVT THARGLGDSA EFQLLSDLLR NGANAPTFEE IFREILDYDE NVRDLLLKNP EDATVAEERR EHERLAGEEL KAAILKMRYD AYSERLTQLS RQSSLTPEEL KEYQELDRTR ADMKRQRGQ

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtga ttccgcattc cttcctgcaa gacctgctta accgcgtcga tatcgtcgac gtggtcggca agtacgtgca gctgaaaaag ggcggcgcga acttcatggg actctgcccg tttcacaacg aaaagagccc ttcgttcacc gttagtccga ctaagcagtt ctatcattgt ttcggctgtg gcgcgcatgg caccgccatt ggctttctga tggagcacgc ggggctgtcg tttcccgaag ccgtcaacga tctggcgcaa tcggtcggtc tgaccgtgcc gcaggagcct tcgccgatgc gcggcggcgc ttcgggcggc gccgcttacg gcgcgggcgg cgaagcctat gcgcctgccg tgccgaaggc cgtcacgacg gcgctgtccg acgtcatgca gacggccagc gagttctaca aggcgcagtt gcgcggcgcg ccgaacgcga tccagtacct gaaaaagcgc ggcctcactg gtgaaatcgc gaaacgcttc ggcctcggct acgcgcccga cggctggcag aacctcgaag ccgcattccc caactatcgg gacgatgcgc tcgtcgagtc gggcctcgtg attgtcagcg agaagtcgga cgcgcagggt cagaaccgcc gctatgaccg tttccgcgag cgcgtgatgt tcccgatccg caacgtgaaa ggccaggtca tcggcttcgg cggacgcgtg ctcgacggcg gcgagccgaa gtatttgaat tctcccgaaa cgcctttatt taacaaaggc agcgaactgt acggtctgtt cgaggcgcgg ctcgcgatcc gcgagcagaa gtacgtgctg gtcgtcgaag ggtatatgga cgtggtcgcg ctggcgcagt tgggcttcga gaacgcggtt gcgacgctcg gcaccgcctg tacgccgatt catgtgcaga aactgatgcg gcagacggac acggtagtgt tcagcttcga cggcgactcg gcgggccggc gcgcggcgcg gcgcgcgctc gacgcgtgcc tgccgcatgc cgccgacaat cggaccatcc gtttcctgtt cctgccggcc gagcatgatc ccgacagcta tgtgcgcgag ttcggcacgc aagcgtttgc ggaacaggtg cgccgcgcga tgccgctgtc acagttcctg ctgaacgaag tgctggcggg caaggaactc gaccagcccg aaggccgcgc acgagcgctg ttcgacgcga agccgctgct gcagatgctt ccggccaatg cgctgcgcgc acagatcatg cacatgttcg cggaccggct cgacgtgccg ttcgaggaag tcgcagcgtt atgcgaagtc gatgcgcgga tcgccgctgt agcacgcagc gcgcctgctc gaaaagaccg gcgcagcgtg acgggtctgg agcagcgggc gctgcgcaat ctcgtcatgc acccgcgcat cgtcgccgtg ctcgacgaag aggaagagcg ggcgctgctg actgtcacac ggcacggcga actgttcgag gaagtcacga cgcatgcccg cgggctgggc gactcggctg aattccagtt gctgtccgac ctgttgcgaa acggggctaa tgcccccacg ttcgaggaaa tcttccggga aattctggac tatgatgaaa acgtccggga tctgctactg aagaatcccg aagacgcgac ggttgccgaa gagcgacgtg agcacgagcg ccttgccggg gaagaattga aagcggccat tctgaaaatg cgctatgacg catacagcga acggctgacc caactgtcgc gccagtcgag cctcacaccg gaggaactga aggagtatca ggaacttgac aggacgcgtg ccgacatgaa gcgtcagcga gggcagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t