SSGCID: Target Page

BuphA.00148.i

Integrase family protein

CENTER ID:	BuphA.00148.i
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00148.i.B1.GE40985	Full length( BuphA.00148.i )		1	343

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.7888
RefSeq:	YP_001863594.1
UniProt:	B2JY36

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKPRKPTELG RCLLRFFQDY LPTLRGVSVH TIRSYRDAVV LLLRFTARES RCPIEALDLS DLNADHVKRF LKFLESERGN SIATRNARLA AIHAFARFVI AEHPEHLAPF QQVLGIPFKR GAREAPIEYL ETAEIETLLT TIDQSRPAGQ RDFALFALMF NTGARVQEIL NLRICDLRLD PPPQVRLRGK GNKVRVCPIW PRTAQLLREL IRTRPLVAQG SAELPVFVNC RGTQMSRFGV RYLLRKYMLI ASTRNPTLAH KEIHPHSLRH TTAIQLLKAG VDFATISQWL GHASLNTTMR YARADIDLKR QALAQVFPEI LAPPRGGAFI LNGADLVGWL RRL

NT Sequence

ATGAAACCGC GCAAACCGAC AGAGCTCGGC CGATGTCTGC TGCGCTTCTT CCAGGACTAT CTGCCTACAC TGCGCGGCGT CAGTGTGCAC ACGATACGGA GCTACCGTGA TGCCGTCGTC TTGCTGCTGC GGTTCACCGC GCGCGAGAGC CGGTGCCCCA TCGAAGCCCT GGATCTGAGC GATCTGAATG CCGATCACGT AAAGCGATTC CTCAAATTCC TTGAGTCCGA GCGCGGCAAC AGCATCGCGA CGCGCAACGC TCGGCTCGCC GCCATTCATG CATTCGCGCG TTTCGTGATT GCCGAGCATC CCGAACATCT GGCACCGTTT CAGCAGGTAC TCGGAATACC GTTTAAGCGG GGAGCGCGGG AGGCACCGAT CGAATATCTG GAAACGGCCG AGATCGAGAC ATTGCTGACC ACCATCGACC AGAGTCGTCC AGCGGGGCAA CGCGACTTTG CACTGTTTGC GCTGATGTTC AACACTGGAG CTCGTGTGCA GGAGATCCTC AATTTGCGGA TCTGTGACCT GCGGCTCGAC CCACCGCCGC AGGTCCGGCT GCGCGGCAAA GGGAACAAGG TGAGAGTGTG TCCGATCTGG CCGCGTACTG CGCAGCTTCT GCGCGAACTT ATTCGCACTC GTCCCTTGGT CGCTCAAGGC TCGGCCGAAC TGCCTGTCTT CGTTAATTGT CGCGGTACTC AGATGAGCCG ATTTGGCGTG CGTTATCTGC TGCGCAAATA CATGCTCATC GCGAGCACCC GGAACCCGAC CCTAGCGCAC AAAGAAATCC ACCCGCATTC GCTTAGGCAT ACCACCGCGA TTCAGTTGCT GAAGGCTGGC GTAGACTTCG CGACGATCAG CCAGTGGCTC GGCCACGCGA GCCTGAATAC CACGATGCGC TATGCGCGTG CCGATATCGA TCTCAAACGT CAAGCACTCG CGCAGGTGTT CCCAGAAATC CTCGCGCCTC CACGAGGTGG AGCATTCATC TTGAACGGTG CTGACCTGGT TGGCTGGCTG CGTAGACTC

Details for BuphA.00148.i.B1.GE40985

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMK PRKPTELGRC LLRFFQDYLP TLRGVSVHTI RSYRDAVVLL LRFTARESRC PIEALDLSDL NADHVKRFLK FLESERGNSI ATRNARLAAI HAFARFVIAE HPEHLAPFQQ VLGIPFKRGA REAPIEYLET AEIETLLTTI DQSRPAGQRD FALFALMFNT GARVQEILNL RICDLRLDPP PQVRLRGKGN KVRVCPIWPR TAQLLRELIR TRPLVAQGSA ELPVFVNCRG TQMSRFGVRY LLRKYMLIAS TRNPTLAHKE IHPHSLRHTT AIQLLKAGVD FATISQWLGH ASLNTTMRYA RADIDLKRQA LAQVFPEILA PPRGGAFILN GADLVGWLRR L

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac cgcgcaaacc gacagagctc ggccgatgtc tgctgcgctt cttccaggac tatctgccta cactgcgcgg cgtcagtgtg cacacgatac ggagctaccg tgatgccgtc gtcttgctgc tgcggttcac cgcgcgcgag agccggtgcc ccatcgaagc cctggatctg agcgatctga atgccgatca cgtaaagcga ttcctcaaat tccttgagtc cgagcgcggc aacagcatcg cgacgcgcaa cgctcggctc gccgccattc atgcattcgc gcgtttcgtg attgccgagc atcccgaaca tctggcaccg tttcagcagg tactcggaat accgtttaag cggggagcgc gggaggcacc gatcgaatat ctggaaacgg ccgagatcga gacattgctg accaccatcg accagagtcg tccagcgggg caacgcgact ttgcactgtt tgcgctgatg ttcaacactg gagctcgtgt gcaggagatc ctcaatttgc ggatctgtga cctgcggctc gacccaccgc cgcaggtccg gctgcgcggc aaagggaaca aggtgagagt gtgtccgatc tggccgcgta ctgcgcagct tctgcgcgaa cttattcgca ctcgtccctt ggtcgctcaa ggctcggccg aactgcctgt cttcgttaat tgtcgcggta ctcagatgag ccgatttggc gtgcgttatc tgctgcgcaa atacatgctc atcgcgagca cccggaaccc gaccctagcg cacaaagaaa tccacccgca ttcgcttagg cataccaccg cgattcagtt gctgaaggct ggcgtagact tcgcgacgat cagccagtgg ctcggccacg cgagcctgaa taccacgatg cgctatgcgc gtgccgatat cgatctcaaa cgtcaagcac tcgcgcaggt gttcccagaa atcctcgcgc ctccacgagg tggagcattc atcttgaacg gtgctgacct ggttggctgg ctgcgtagac tctgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat