SSGCID: Target Page

BuphA.00148.a

Tyrosine recombinase XerD

CENTER ID:	BuphA.00148.a
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00148.a.B1.GE40278	Full length( BuphA.00148.a )		1	315

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.549
RefSeq:	YP_001856761.1
UniProt:	B2JDT9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDVLTEDAQ PASPAVSPLL LTSGASIDAF CDALWLEHGL ARNTLDAYRR DLRLFSEWLA ERRDASLDAA SEADLTAYSA ARSADKSTSA NRRLSVFRRY YAWAVREHRA SADPTLRIRS AKQPPRFPST LTEAQVEALL GAPDVTTPLG LRDRTMLELM YASGLRVSEL VTLKTVEVGL NEGVVRVMGK GSKERLIPFG EEAHAWIERY LRESRPALLG QRSADALFVT ARAEGMTRQQ FWNIIKRHAL TAHVHAPLSP HTLRHAFATH LLNHGADLRV VQLLLGHTDI STTQIYTHVA RERLKSLHAA HHPRG

NT Sequence

ATGACTGATG TGCTGACCGA AGACGCGCAG CCCGCGTCGC CTGCTGTTTC GCCGCTGCTG CTGACGAGCG GCGCGTCGAT CGATGCCTTC TGCGATGCGC TATGGCTCGA ACATGGCCTC GCGCGCAACA CGCTCGACGC ATACCGACGC GACTTGCGGC TCTTTTCCGA ATGGCTCGCC GAGCGGCGCG ACGCTTCTCT CGATGCCGCG AGCGAAGCCG ATCTGACGGC CTATAGCGCC GCGCGCTCGG CCGACAAGTC GACCTCCGCG AACCGGCGTC TGTCTGTGTT TCGCCGCTAC TATGCGTGGG CCGTGCGCGA GCATCGCGCG TCAGCGGATC CAACCTTGCG CATCCGTTCG GCCAAGCAGC CGCCGCGTTT CCCGTCGACC TTGACGGAAG CGCAGGTCGA AGCGCTGCTC GGGGCGCCGG ACGTGACGAC GCCGCTCGGC CTGCGCGACC GCACGATGCT CGAACTGATG TACGCGAGCG GCTTGCGCGT CAGCGAACTG GTCACGTTGA AGACGGTGGA GGTCGGGCTG AACGAGGGCG TCGTGCGCGT GATGGGCAAG GGCTCGAAAG AGCGGCTGAT ACCGTTCGGC GAAGAGGCGC ACGCCTGGAT TGAACGCTAT CTGCGCGAGT CTCGCCCCGC ATTGCTCGGC CAGCGTTCCG CTGATGCGCT GTTCGTCACC GCGCGCGCCG AAGGCATGAC GCGCCAGCAG TTCTGGAACA TCATCAAGCG TCATGCGCTG ACGGCGCATG TGCACGCGCC CCTGTCACCG CACACGCTGC GGCACGCGTT CGCGACGCAT CTGCTGAATC ACGGCGCGGA CCTGCGTGTC GTGCAGCTGC TGCTCGGCCA CACCGATATC TCGACCACGC AGATTTACAC GCACGTCGCA CGTGAGCGGC TCAAGTCGCT GCACGCGGCT CACCATCCGC GCGGC

Details for BuphA.00148.a.B1.GE40278

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/20/2016
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	59
PERCENT COVERAGE:	49

Validated AA Sequence

MRXXXXXXXR XXRSXXXDAT CGSXXXXSPS GATLLSMXRA XXIXXXIXXX XRPTSRPPRT XVCLCFAAXN XXAVREHRAS ADPTLRIRSA KQPPRFPSTX XEAQVEALLG APDVTTPLGL RDRTMLELMY ASGLRVSELV TLKTVEVGLN EGVVRVMGKG S

Validated NT Sequence

tttcgagccc ttgcccatca cgcgcacgac gccctcgttc agcccgacct ccaccgtctt caacgtgacc agttcgctga cgcgcaagcc gctcgcgtac atcagttcga gcatcgtgcg gtcgcgcagg ccgagcggcg tcgtcacgtc cggcgccccg agcagcgctt cgacctgcgc ttccnnnnnn gtcgacggga aacgcggcgg ctgcttggcc gaacggatgc gcaaggttgg atccgctgac gcgcgatgct cgcgcacggc nnnngnatta gnngcggcga aacacagaca gacnncggtt cgcggaggtc gacttgtcgg ccgagnnnnn nncnctatag nnnnncagat nnnnntngct cgnnncatcg agagaagcgt cgcgccgctc ggcgagncnn ncnnnnaaga gccgcaagtc gcgtcnnnnn nnnnngagcg tgnnnnncgc nnngnnnnnn nnnancnnna ncgcatcgca gaaggcannn nnnnncgcgc cgctcgtcag cagcagcggc gaaacagcag gcgacgcggg ctgcgnnnnn ncngtnnnca catcagtcat anngnnnnnn nnnnggtgag ccnngnnntn tnt

Expected Protein Sequence

MAHHHHHHMT DVLTEDAQPA SPAVSPLLLT SGASIDAFCD ALWLEHGLAR NTLDAYRRDL RLFSEWLAER RDASLDAASE ADLTAYSAAR SADKSTSANR RLSVFRRYYA WAVREHRASA DPTLRIRSAK QPPRFPSTLT EAQVEALLGA PDVTTPLGLR DRTMLELMYA SGLRVSELVT LKTVEVGLNE GVVRVMGKGS KERLIPFGEE AHAWIERYLR ESRPALLGQR SADALFVTAR AEGMTRQQFW NIIKRHALTA HVHAPLSPHT LRHAFATHLL NHGADLRVVQ LLLGHTDIST TQIYTHVARE RLKSLHAAHH PRG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgactg atgtgctgac cgaagacgcg cagcccgcgt cgcctgctgt ttcgccgctg ctgctgacga gcggcgcgtc gatcgatgcc ttctgcgatg cgctatggct cgaacatggc ctcgcgcgca acacgctcga cgcataccga cgcgacttgc ggctcttttc cgaatggctc gccgagcggc gcgacgcttc tctcgatgcc gcgagcgaag ccgatctgac ggcctatagc gccgcgcgct cggccgacaa gtcgacctcc gcgaaccggc gtctgtctgt gtttcgccgc tactatgcgt gggccgtgcg cgagcatcgc gcgtcagcgg atccaacctt gcgcatccgt tcggccaagc agccgccgcg tttcccgtcg accttgacgg aagcgcaggt cgaagcgctg ctcggggcgc cggacgtgac gacgccgctc ggcctgcgcg accgcacgat gctcgaactg atgtacgcga gcggcttgcg cgtcagcgaa ctggtcacgt tgaagacggt ggaggtcggg ctgaacgagg gcgtcgtgcg cgtgatgggc aagggctcga aagagcggct gataccgttc ggcgaagagg cgcacgcctg gattgaacgc tatctgcgcg agtctcgccc cgcattgctc ggccagcgtt ccgctgatgc gctgttcgtc accgcgcgcg ccgaaggcat gacgcgccag cagttctgga acatcatcaa gcgtcatgcg ctgacggcgc atgtgcacgc gcccctgtca ccgcacacgc tgcggcacgc gttcgcgacg catctgctga atcacggcgc ggacctgcgt gtcgtgcagc tgctgctcgg ccacaccgat atctcgacca cgcagattta cacgcacgtc gcacgtgagc ggctcaagtc gctgcacgcg gctcaccatc cgcgcggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat