BuphA.00141.b

Malonate decarboxylase, epsilon subunit

CENTER ID: BuphA.00141.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00141.b.B1.GE39392 Full length( BuphA.00141.b ) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.5348
RefSeq: YP_001861214.1
UniProt: B2JLU0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLAYLFPGQG AQSEGFLRHL GAHPAIRATL AEASDVLGVD VLTLDTRAAL ESTVAVQTGL LIAGVATQRA LAEEGLFPEL SAGLSVGAYA AAVSCCAIEF ADALRMVRKR AELMESAYPQ GYGLAAIAGL TEHEVEMLAD AQAQASHDRV YVGNVNAPRQ IVVAGSDAAL DSFNERALAA GARKAMRLAV SVPSHCELLA AAADALIDYA RSVPFRAPSS GYIGNRGGRA LHTADAVRDD LATNMRYTVR WFDALTVMVE MGATVFVEAP PGQVLTDIVR EQYPDTAAIA ANTLPFERLP PTIQRRMEST D
NT Sequence
ATGCTGGCCT ATCTCTTTCC CGGCCAGGGC GCGCAATCCG AAGGCTTTTT GCGGCACCTC GGCGCGCATC CCGCCATTCG GGCCACGCTC GCCGAAGCAT CCGACGTGCT CGGCGTCGAC GTGCTCACGC TCGACACGCG CGCCGCGCTC GAATCGACCG TCGCTGTGCA GACGGGGCTG CTGATCGCAG GCGTGGCGAC GCAGCGCGCG CTCGCCGAGG AAGGCCTGTT TCCCGAACTC AGCGCGGGCC TGTCGGTCGG CGCGTACGCG GCCGCTGTGA GCTGTTGCGC GATCGAGTTC GCCGACGCGC TGCGCATGGT GCGCAAGCGC GCTGAACTGA TGGAGAGCGC GTATCCGCAA GGGTACGGCC TCGCGGCGAT TGCCGGGCTG ACGGAACACG AGGTCGAAAT GCTCGCCGAT GCACAGGCGC AGGCGTCACA CGACCGCGTG TACGTCGGCA ATGTGAATGC GCCACGCCAG ATCGTCGTCG CAGGCTCGGA TGCCGCGCTC GATAGCTTTA ACGAACGCGC ACTCGCAGCC GGCGCGCGCA AGGCGATGCG GCTCGCCGTG AGCGTGCCGT CGCATTGCGA ACTGCTCGCC GCAGCCGCCG ACGCGTTGAT CGACTACGCG CGCAGCGTGC CGTTTCGCGC GCCGTCGAGC GGCTACATCG GCAATCGCGG GGGCCGCGCG CTTCATACGG CCGACGCCGT CCGCGACGAT CTCGCCACCA ACATGCGCTA CACGGTCCGC TGGTTCGACG CGCTGACCGT AATGGTCGAA ATGGGCGCAA CCGTGTTCGT GGAAGCGCCA CCCGGCCAGG TGCTGACGGA CATCGTTCGC GAGCAGTATC CCGACACGGC CGCGATCGCA GCGAATACGC TGCCGTTCGA GCGGCTCCCG CCGACCATTC AGCGACGCAT GGAATCGACT GAC
Details for BuphA.00141.b.B1.GE39392
HARVESTED ON: 8/5/2015
SEQUENCED ON: 8/13/2015
EXPECTED MW: 34kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML AYLFPGQGAQ SEGFLRHLGA HPAIRATLAE ASDVLGVDVL TLDTRAALES TVAVQTGLLI AGVATQRALA EEGLFPELSA GLSVGAYAAA VSCCAIEFAD ALRMVRKRAE LMESAYPQGY GLAAIAGLTE HEVEMLADAQ AQASHDRVYV GNVNAPRQIV VAGSDAALDS FNERALAAGA RKAMRLAVSV PSHCELLAAA ADALIDYARS VPFRAPSSGY IGNRGGRALH TADAVRDDLA TNMRYTVRWF DALTVMVEMG ATVFVEAPPG QVLTDIVREQ YPDTAAIAAN TLPFERLPPT IXXXMESTD
Validated NT Sequence
ttactcactt agtcagtcga ttccatgnnn nnnngaatgg tcggcgggag ccgctcgaac ggcagcgtat tcgctgcgat cgcggccgtg tcgggatact gctcgcgaac gatgtccgtc agcacctggc cgggtggcgc ttccacgaac acggttgcgc ccatttcgac cattacggtc agcgcgtcga accagcggac cgtgtagcgc atgttggtgg cgagatcgtc gcggacggcg tcggccgtat gaagcgcgcg gcccccgcga ttgccgatgt agccgctcga cggcgcgcga aacggcacgc tgcgcgcgta gtcgatcaac gcgtcggcgg ctgcggcgag cagttcgcaa tgcgacggca cgctcacggc gagccgcatc gccttgcgcg cgccggctgc gagtgcgcgt tcgttaaagc tatcgagcgc ggcatccgag cctgcgacga cgatctggcg tggcgcattc acattgccga cgtacacgcg gtcgtgtgac gcctgcgcct gtgcatcggc gagcatttcg acctcgtgtt ccgtcagccc ggcaatcgcc gcgaggccgt acccttgcgg atacgcgctc tccatcagtt cagcgcgctt gcgcaccatg cgcagcgcgt cggcgaactc gatcgcgcaa cagctcacag cggccgcgta cgcgccgacc gacaggcccg cgctgagttc gggaaacagg ccttcctcgg cgagcgcgcg ctgcgtcgcc acgcctgcga tcagcagccc cgtctgcaca gcgacggtcg attcgagcgc ggcgcgcgtg tcgagcgtga gcacgtcgac gccgagcacg tcggatgctt cggcgagcgt ggcccgaatg gcgggatgcg cgccgaggtg ccgcaaaaag ccttcggatt gcgcgccctg gccgggaaag agataggcca gcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHML AYLFPGQGAQ SEGFLRHLGA HPAIRATLAE ASDVLGVDVL TLDTRAALES TVAVQTGLLI AGVATQRALA EEGLFPELSA GLSVGAYAAA VSCCAIEFAD ALRMVRKRAE LMESAYPQGY GLAAIAGLTE HEVEMLADAQ AQASHDRVYV GNVNAPRQIV VAGSDAALDS FNERALAAGA RKAMRLAVSV PSHCELLAAA ADALIDYARS VPFRAPSSGY IGNRGGRALH TADAVRDDLA TNMRYTVRWF DALTVMVEMG ATVFVEAPPG QVLTDIVREQ YPDTAAIAAN TLPFERLPPT IQRRMESTD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgg cctatctctt tcccggccag ggcgcgcaat ccgaaggctt tttgcggcac ctcggcgcgc atcccgccat tcgggccacg ctcgccgaag catccgacgt gctcggcgtc gacgtgctca cgctcgacac gcgcgccgcg ctcgaatcga ccgtcgctgt gcagacgggg ctgctgatcg caggcgtggc gacgcagcgc gcgctcgccg aggaaggcct gtttcccgaa ctcagcgcgg gcctgtcggt cggcgcgtac gcggccgctg tgagctgttg cgcgatcgag ttcgccgacg cgctgcgcat ggtgcgcaag cgcgctgaac tgatggagag cgcgtatccg caagggtacg gcctcgcggc gattgccggg ctgacggaac acgaggtcga aatgctcgcc gatgcacagg cgcaggcgtc acacgaccgc gtgtacgtcg gcaatgtgaa tgcgccacgc cagatcgtcg tcgcaggctc ggatgccgcg ctcgatagct ttaacgaacg cgcactcgca gccggcgcgc gcaaggcgat gcggctcgcc gtgagcgtgc cgtcgcattg cgaactgctc gccgcagccg ccgacgcgtt gatcgactac gcgcgcagcg tgccgtttcg cgcgccgtcg agcggctaca tcggcaatcg cgggggccgc gcgcttcata cggccgacgc cgtccgcgac gatctcgcca ccaacatgcg ctacacggtc cgctggttcg acgcgctgac cgtaatggtc gaaatgggcg caaccgtgtt cgtggaagcg ccacccggcc aggtgctgac ggacatcgtt cgcgagcagt atcccgacac ggccgcgatc gcagcgaata cgctgccgtt cgagcggctc ccgccgacca ttcagcgacg catggaatcg actgactgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t