BuphA.00137.b

UDP-N-acetylmuramate--L-alanine ligase

CENTER ID: BuphA.00137.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00137.b.B1.GE35968 Full length( BuphA.00137.b ) 1 464
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.2834
RefSeq: YP_001858890.1
UniProt: B2JHF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKHIVKHIHF VGIGGAGMSG IAEVLVNLGY QVSGSDLSRN AVTERLAALG ARIAIGHDAA NIEGANAVVV STAVRSDNPE VLAARHRRIP IVPRAVMLAE LMRLKQGIAI AGTHGKTTTT SLVASVLAAG GLDPTFVIGG RLISAGANAR LGTGDFIVAE ADESDASFLN LFPVIEVITN IDADHMDTYG HDFARLKQAF IEFTHRLPFY GIAVLCVDDP NVKEILPFVS KPIIRYGFAP DAQVRAVNVE AREGKMHFTA MREDAPPIDI VLNLPGLHNV QNALAAIAIA TELEVKDADI QRALADFNGV GRRFQRYGEV TVTSGGAYTL VDDYGHHPVE MAATIAAARG AFPDKRLVLA FQPHRFTRTR DCFEDFVKVL STVDALVLTE VYSAGEAPIV AADGRSLTRA IRVAGKVEPV FVDTVDEVPD ALGAIVRDGD VVITMGAGSI GSVPGRLAGN EGVK
NT Sequence
ATGAAACATA TCGTCAAACA CATTCACTTC GTCGGGATCG GCGGCGCGGG CATGAGCGGC ATCGCCGAAG TGCTCGTCAA TCTCGGCTAT CAGGTGAGCG GATCGGACCT GTCGCGCAAC GCGGTGACGG AGCGCCTCGC CGCGCTCGGT GCGCGCATCG CGATCGGCCA CGACGCAGCG AACATCGAAG GCGCGAACGC CGTCGTCGTC TCCACGGCCG TGCGCAGCGA CAACCCTGAA GTGCTGGCCG CGCGCCATCG CCGCATTCCT ATCGTGCCGC GCGCCGTGAT GCTTGCGGAA CTGATGCGCC TGAAGCAGGG CATCGCGATC GCGGGCACGC ACGGCAAGAC CACGACCACC TCGCTGGTGG CGAGCGTGCT GGCGGCGGGC GGTCTCGATC CGACCTTCGT GATCGGCGGC CGGCTGATCA GCGCCGGCGC GAATGCGCGT CTCGGCACGG GCGATTTCAT CGTCGCCGAA GCGGACGAAT CCGATGCGTC GTTCCTGAAC CTGTTTCCGG TGATCGAAGT CATCACGAAC ATCGACGCCG ATCACATGGA CACCTACGGC CACGACTTCG CGCGGCTGAA GCAGGCGTTC ATTGAATTTA CGCATCGTCT GCCGTTTTAT GGCATTGCGG TGCTGTGCGT CGACGATCCG AACGTGAAGG AGATTCTGCC GTTCGTGTCG AAGCCGATCA TCCGCTACGG CTTCGCGCCC GATGCGCAGG TGCGCGCGGT GAACGTCGAA GCGCGCGAAG GCAAGATGCA TTTCACGGCG ATGCGCGAGG ATGCGCCGCC CATCGACATC GTGTTGAACC TGCCGGGTCT GCACAACGTG CAGAACGCGC TCGCCGCGAT TGCGATTGCG ACTGAACTTG AGGTGAAAGA TGCCGATATC CAGCGAGCGC TCGCGGATTT CAACGGCGTC GGCCGGCGTT TTCAGCGTTA CGGGGAAGTG ACCGTGACGA GCGGCGGCGC GTACACGCTC GTCGACGACT ACGGCCATCA CCCGGTCGAA ATGGCGGCGA CGATTGCGGC GGCGCGCGGT GCATTTCCGG ACAAGCGTCT CGTGCTCGCG TTCCAGCCTC ACCGGTTCAC GCGCACGCGC GACTGCTTCG AAGATTTCGT GAAGGTGCTG TCGACGGTCG ACGCGCTCGT GCTGACGGAA GTCTATTCGG CGGGCGAAGC GCCCATCGTC GCGGCGGACG GCCGTTCGCT CACGCGCGCG ATCCGCGTGG CGGGCAAGGT GGAGCCGGTG TTTGTCGACA CAGTGGATGA AGTGCCGGAT GCGCTCGGTG CAATCGTGCG CGACGGCGAT GTGGTGATCA CGATGGGTGC GGGTTCGATC GGGAGTGTGC CGGGTCGGCT CGCAGGAAAC GAAGGTGTGA AA
Details for BuphA.00137.b.B1.GE35968
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 73
Validated AA Sequence
MSGIAEVLVN LGYQVSGSDL SRNAVTERLA ALGARIAIGH DAANIEGANA VVVSTAVRSD NPEVLAARHR RIPIVPRAVM LAELMRLKQG IAIAGTHGKT TTTSLVASVL AAGGLDPTFV IGGRLISAGA NARLGTGDFI VAEADESDAS FLNLFPVIEV ITNIDADHMD TYGHDFARLK QAFIEFTHRL PFYGIAVLCV DDPNVKEILP FVSKPIIRYG FAPDAQVRAV NVEAREGKMH FTAMREDAPP IDIVLNLPGL HNVQNALAAI AIATELEVKD ADIQRALADF NGVGRRFQRY GEVTVTSGGA YTLVDDYGHH PVEMAATIAA ARGAFPDKRL VLNXT
Validated NT Sequence
tgagtcntat taagcacgag acgcttgtcc ggaaatgcac cgcgcgccgc cgcaatcgtc gccgccattt cgaccgggtg atggccgtag tcgtcgacga gcgtgtacgc gccgccgctc gtcacggtca cttccccgta acgctgaaaa cgccggccga cgccgttgaa atccgcgagc gctcgctgga tatcggcatc tttcacctca agttcagtcg caatcgcaat cgcggcgagc gcgttctgca cgttgtgcag acccggcagg ttcaacacga tgtcgatggg cggcgcatcc tcgcgcatcg ccgtgaaatg catcttgcct tcgcgcgctt cgacgttcac cgcgcgcacc tgcgcatcgg gcgcgaagcc gtagcggatg atcggcttcg acacgaacgg cagaatctcc ttcacgttcg gatcgtcgac gcacagcacc gcaatgccat aaaacggcag acgatgcgta aattcaatga acgcctgctt cagccgcgcg aagtcgtggc cgtaggtgtc catgtgatcg gcgtcgatgt tcgtgatgac ttcgatcacc ggaaacaggt tcaggaacga cgcatcggat tcgtccgctt cggcgacgat gaaatcgccc gtgccgagac gcgcattcgc gccggcgctg atcagccggc cgccgatcac gaaggtcgga tcgagaccgc ccgccgccag cacgctcgcc accagcgagg tggtcgtggt cttgccgtgc gtgcccgcga tcgcgatgcc ctgcttcagg cgcatcagtt ccgcaagcat cacggcgcgc ggcacgatag gaatgcggcg atggcgcgcg gccagcactt cagggttgtc gctgcgcacg gccgtggaga cgacgacggc gttcgcgcct tcgatgttcg ctgcgtcgtg gccgatcgcg atgcgcgcac cgagcgcggc gaggcgctcc gtcaccgcgt tgcgcgacag gtccgatccg ctcacctgat agccgagatt gacgagcact tcggcgatgc cgctcatgcc cgcgccgccg atcccgacga agtgaatgtg tttgac
Expected Protein Sequence
MAHHHHHHMK HIVKHIHFVG IGGAGMSGIA EVLVNLGYQV SGSDLSRNAV TERLAALGAR IAIGHDAANI EGANAVVVST AVRSDNPEVL AARHRRIPIV PRAVMLAELM RLKQGIAIAG THGKTTTTSL VASVLAAGGL DPTFVIGGRL ISAGANARLG TGDFIVAEAD ESDASFLNLF PVIEVITNID ADHMDTYGHD FARLKQAFIE FTHRLPFYGI AVLCVDDPNV KEILPFVSKP IIRYGFAPDA QVRAVNVEAR EGKMHFTAMR EDAPPIDIVL NLPGLHNVQN ALAAIAIATE LEVKDADIQR ALADFNGVGR RFQRYGEVTV TSGGAYTLVD DYGHHPVEMA ATIAAARGAF PDKRLVLAFQ PHRFTRTRDC FEDFVKVLST VDALVLTEVY SAGEAPIVAA DGRSLTRAIR VAGKVEPVFV DTVDEVPDAL GAIVRDGDVV ITMGAGSIGS VPGRLAGNEG VK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaac atatcgtcaa acacattcac ttcgtcggga tcggcggcgc gggcatgagc ggcatcgccg aagtgctcgt caatctcggc tatcaggtga gcggatcgga cctgtcgcgc aacgcggtga cggagcgcct cgccgcgctc ggtgcgcgca tcgcgatcgg ccacgacgca gcgaacatcg aaggcgcgaa cgccgtcgtc gtctccacgg ccgtgcgcag cgacaaccct gaagtgctgg ccgcgcgcca tcgccgcatt cctatcgtgc cgcgcgccgt gatgcttgcg gaactgatgc gcctgaagca gggcatcgcg atcgcgggca cgcacggcaa gaccacgacc acctcgctgg tggcgagcgt gctggcggcg ggcggtctcg atccgacctt cgtgatcggc ggccggctga tcagcgccgg cgcgaatgcg cgtctcggca cgggcgattt catcgtcgcc gaagcggacg aatccgatgc gtcgttcctg aacctgtttc cggtgatcga agtcatcacg aacatcgacg ccgatcacat ggacacctac ggccacgact tcgcgcggct gaagcaggcg ttcattgaat ttacgcatcg tctgccgttt tatggcattg cggtgctgtg cgtcgacgat ccgaacgtga aggagattct gccgttcgtg tcgaagccga tcatccgcta cggcttcgcg cccgatgcgc aggtgcgcgc ggtgaacgtc gaagcgcgcg aaggcaagat gcatttcacg gcgatgcgcg aggatgcgcc gcccatcgac atcgtgttga acctgccggg tctgcacaac gtgcagaacg cgctcgccgc gattgcgatt gcgactgaac ttgaggtgaa agatgccgat atccagcgag cgctcgcgga tttcaacggc gtcggccggc gttttcagcg ttacggggaa gtgaccgtga cgagcggcgg cgcgtacacg ctcgtcgacg actacggcca tcacccggtc gaaatggcgg cgacgattgc ggcggcgcgc ggtgcatttc cggacaagcg tctcgtgctc gcgttccagc ctcaccggtt cacgcgcacg cgcgactgct tcgaagattt cgtgaaggtg ctgtcgacgg tcgacgcgct cgtgctgacg gaagtctatt cggcgggcga agcgcccatc gtcgcggcgg acggccgttc gctcacgcgc gcgatccgcg tggcgggcaa ggtggagccg gtgtttgtcg acacagtgga tgaagtgccg gatgcgctcg gtgcaatcgt gcgcgacggc gatgtggtga tcacgatggg tgcgggttcg atcgggagtg tgccgggtcg gctcgcagga aacgaaggtg tgaaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat