BuphA.00137.a

UDP-N-acetylmuramate

CENTER ID: BuphA.00137.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00137.a.B2.GE35967 Mature protein( BuphA.00137.a ) 20 469
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.2785
RefSeq: YP_001858843.1
UniProt: B2JH13

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHIHILGICG TFMGGLAVLA RNAGHTVTGC DAGVYPPMST QLEAQGIKLI EGWGVEQLDL KPDLFVVGNV VTRGNPLMEE ILNRGLPYTS GPQWLGEHVL NGKWVLAVAG THGKTTTTSM LTWLLEDAGM NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEFDH ADIFPDLAAI ETQFHHLVRT VPGIGRVVSN GREVALDRVL ARGCWSEVER FGVEGGWQAL PAEDGVAVDE RFAVYHNGER VGVVDWQVQG EHNRMNAIAA IAAARHIGVP PAQAAKSLST FRNVKRRMEV RGSVDGVTVY DDFAHHPTAI QTTVAGLRTR IGRTNTRILA VLEPRSNTMK LGVMKAQLPA SLADADLVFG YGAPSGKDAL GWNLAEALAP MGDKAQAFNE IDSLVKAVVA AAQPGDQVLV MSNGGFGGVH QKLLDALSAR PTEQARGVA
NT Sequence
ATGCACATTC ACATCCTCGG CATCTGCGGC ACATTCATGG GTGGTCTCGC GGTTCTCGCG CGCAACGCCG GTCACACGGT GACGGGCTGC GACGCCGGCG TCTATCCGCC GATGAGCACG CAGCTCGAGG CGCAAGGCAT CAAGCTGATC GAAGGCTGGG GCGTCGAGCA ACTCGATCTG AAGCCGGACC TGTTCGTGGT CGGCAACGTC GTGACGCGCG GCAATCCGCT GATGGAAGAG ATCCTCAATC GAGGACTGCC GTACACGTCC GGCCCGCAGT GGCTCGGCGA GCATGTGCTT AACGGCAAAT GGGTGCTGGC CGTGGCAGGC ACGCACGGCA AGACCACGAC GACGTCGATG CTGACGTGGC TGCTCGAAGA CGCGGGCATG AACCCAGGCT TTCTGATCGG CGGCGTGCCG CTGAACTTCG GTGTGTCGGC GCGGCTGACG GATTCCAGCT TTTTCGTGAT CGAAGCCGAC GAATACGACA CCGCGTTCTT CGACAAGCGC TCGAAATTCG TCCACTACCG GCCGCGCACC GCCGTTCTGA ACAACCTCGA ATTCGATCAC GCGGATATCT TCCCCGATCT CGCTGCGATC GAAACGCAAT TCCACCATCT CGTGCGCACC GTGCCGGGCA TTGGCCGCGT CGTGTCGAAC GGCCGTGAAG TTGCACTGGA CCGCGTGCTC GCGCGCGGCT GCTGGAGCGA AGTGGAGCGC TTCGGCGTCG AGGGCGGCTG GCAGGCGTTG CCCGCTGAAG ACGGCGTCGC CGTCGACGAG CGCTTTGCCG TCTATCACAA CGGCGAGCGC GTCGGTGTGG TCGACTGGCA GGTTCAGGGC GAGCACAACC GGATGAACGC GATCGCCGCG ATCGCCGCCG CGCGCCACAT CGGCGTGCCG CCCGCGCAGG CAGCGAAGTC GCTCTCGACG TTCCGCAACG TGAAGCGCCG GATGGAAGTG CGCGGCAGCG TCGACGGCGT GACCGTCTAC GACGATTTCG CGCACCATCC GACGGCGATC CAGACGACGG TCGCGGGTCT GCGCACGCGT ATCGGCCGTA CGAATACACG CATTCTCGCC GTGCTGGAGC CGCGCTCGAA CACAATGAAG CTCGGCGTGA TGAAAGCGCA ACTCCCGGCG AGCCTGGCCG ACGCAGATCT CGTGTTCGGG TACGGCGCAC CTTCGGGCAA GGACGCGCTC GGCTGGAACC TCGCCGAAGC TCTCGCGCCG ATGGGCGACA AGGCGCAGGC GTTCAATGAA ATCGACTCGC TCGTCAAGGC AGTCGTCGCG GCCGCGCAGC CGGGCGACCA GGTGCTGGTG ATGAGCAACG GCGGTTTCGG CGGCGTGCAT CAGAAGCTGC TCGATGCGCT GTCGGCACGG CCGACGGAGC AGGCGCGGGG CGTCGCG
Details for BuphA.00137.a.B2.GE35967
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 92
PERCENT COVERAGE: 46
Validated AA Sequence
MAHHHHHHMA RNAGHTVTGC DAGVYPPMST QLEAQGIKLI EGWGVEQLDL KPDLFVVGNV VTRGNPLMEE ILNRGLPYTS GPQWLGEHVL NGKWVLAVAG THGKTTTTSM LTWLLEDAGM NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEFDH ADIFPDLAAI ETQXPPSRXH RAGXXXXXXX XX
Validated NT Sequence
attttnntta actttaagaa ggaganatac catggctcac caccaccacc accatatggc gcgcaacgcc ggtcacacgg tgacgggctg cgacgccggc gtctatccgc cgatgagcac gcagctcgag gcgcaaggca tcaagctgat cgaaggctgg ggcgtcgagc aactcgatct gaagccggac ctgttcgtgg tcggcaacgt cgtgacgcgc ggcaatccgc tgatggaaga gatcctcaat cgaggactgc cgtacacgtc cggcccgcag tggctcggcg agcatgtgct taacggcaaa tgggtgctgg ccgtggcagg cacgcacggc aagaccacga cgacgtcgat gctgacgtgg ctgctcgaag acgcgggcat gaacccaggc tttctgatcg gcggcgtgcc gctgaacttc ggtgtgtcgg cgcggctgac ggattccagc tttttcgtga tcgaagccga cgaatacgac accgcgttct tcgacaagcg ctcgaaattc gtccactacc ggccgcgcac cgccgttctg aacaacctcg aattcgatca cgcggatatc ttccccgatc tcgctgcgat cgaaacgcaa ttnccaccat ctcgngnnca ccgngccggg nattgnnngc nncnnnncna acnnnngtga anttgnnntg gnccnnnncn nnnnngntgc ngnannnann nnnnnnntnn nnntnnnnng nngnngnnnn ccnnctnaaa annnnnnccc nnnnnnnnnn nnntncnnan nnnnnnnnnn nnnnnnnnna ntgnnnnnnn ngnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngnnnnc cnnnnnnnnn nnnnnannnn nnnnnnnnnn nnnnnnnnnn nnnncnnnnn nnnnnnnnnn nngnncnna
Expected Protein Sequence
MAHHHHHHMA RNAGHTVTGC DAGVYPPMST QLEAQGIKLI EGWGVEQLDL KPDLFVVGNV VTRGNPLMEE ILNRGLPYTS GPQWLGEHVL NGKWVLAVAG THGKTTTTSM LTWLLEDAGM NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEFDH ADIFPDLAAI ETQFHHLVRT VPGIGRVVSN GREVALDRVL ARGCWSEVER FGVEGGWQAL PAEDGVAVDE RFAVYHNGER VGVVDWQVQG EHNRMNAIAA IAAARHIGVP PAQAAKSLST FRNVKRRMEV RGSVDGVTVY DDFAHHPTAI QTTVAGLRTR IGRTNTRILA VLEPRSNTMK LGVMKAQLPA SLADADLVFG YGAPSGKDAL GWNLAEALAP MGDKAQAFNE IDSLVKAVVA AAQPGDQVLV MSNGGFGGVH QKLLDALSAR PTEQARGVA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcgc gcaacgccgg tcacacggtg acgggctgcg acgccggcgt ctatccgccg atgagcacgc agctcgaggc gcaaggcatc aagctgatcg aaggctgggg cgtcgagcaa ctcgatctga agccggacct gttcgtggtc ggcaacgtcg tgacgcgcgg caatccgctg atggaagaga tcctcaatcg aggactgccg tacacgtccg gcccgcagtg gctcggcgag catgtgctta acggcaaatg ggtgctggcc gtggcaggca cgcacggcaa gaccacgacg acgtcgatgc tgacgtggct gctcgaagac gcgggcatga acccaggctt tctgatcggc ggcgtgccgc tgaacttcgg tgtgtcggcg cggctgacgg attccagctt tttcgtgatc gaagccgacg aatacgacac cgcgttcttc gacaagcgct cgaaattcgt ccactaccgg ccgcgcaccg ccgttctgaa caacctcgaa ttcgatcacg cggatatctt ccccgatctc gctgcgatcg aaacgcaatt ccaccatctc gtgcgcaccg tgccgggcat tggccgcgtc gtgtcgaacg gccgtgaagt tgcactggac cgcgtgctcg cgcgcggctg ctggagcgaa gtggagcgct tcggcgtcga gggcggctgg caggcgttgc ccgctgaaga cggcgtcgcc gtcgacgagc gctttgccgt ctatcacaac ggcgagcgcg tcggtgtggt cgactggcag gttcagggcg agcacaaccg gatgaacgcg atcgccgcga tcgccgccgc gcgccacatc ggcgtgccgc ccgcgcaggc agcgaagtcg ctctcgacgt tccgcaacgt gaagcgccgg atggaagtgc gcggcagcgt cgacggcgtg accgtctacg acgatttcgc gcaccatccg acggcgatcc agacgacggt cgcgggtctg cgcacgcgta tcggccgtac gaatacacgc attctcgccg tgctggagcc gcgctcgaac acaatgaagc tcggcgtgat gaaagcgcaa ctcccggcga gcctggccga cgcagatctc gtgttcgggt acggcgcacc ttcgggcaag gacgcgctcg gctggaacct cgccgaagct ctcgcgccga tgggcgacaa ggcgcaggcg ttcaatgaaa tcgactcgct cgtcaaggca gtcgtcgcgg ccgcgcagcc gggcgaccag gtgctggtga tgagcaacgg cggtttcggc ggcgtgcatc agaagctgct cgatgcgctg tcggcacggc cgacggagca ggcgcggggc gtcgcgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t