BuphA.00134.a

4-hydroxyphenylpyruvate dioxygenase

CENTER ID: BuphA.00134.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00134.a.B1.GE40203 Full length( BuphA.00134.a ) 1 375
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00134.a.B1.PS38139 Full length( BuphA.00134.a ) 1 375

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.4106
RefSeq: YP_001860062.1
UniProt: B2JNR5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSDLPTPND ALKAVADPEH NPLGTAGIEF VEFAARHPQM LAETFVKLGF KPIARHISKD VTLFRQADMN FLLNAEPDSF AERYAEEYGV GICAIGIRVA DAQRAYDRAI ELGAWSFEGE RLGKGELVIP AIQGIGDSHI YFIDRWRGRG GQKGGLGDIS IFDIDFRPIE VSTAQADLSH AGTGLVAVDH LTQTVGAGRM QEWIDFYREL LNFREIHELH ANWHVSAESR VMVSPCGAIR IPLYEEGTRR TSLMHEYLPD HPGEGVQHIA LATDDIFACT DQLLANGIEL VEPPPAYYEQ IDARLPGHGL DIERLKRGRI LVDGEIGADG VPLLFFQTFV LRREGDIFFE IVQREGHHGF GEGNLSALAQ ARSAA
NT Sequence
ATGCCCAGCG ACCTGCCGAC CCCCAACGAC GCGCTCAAGG CCGTCGCGGA CCCGGAGCAC AACCCGCTCG GCACAGCCGG CATCGAGTTC GTCGAATTCG CGGCGCGTCA CCCGCAGATG CTGGCGGAGA CGTTCGTGAA GCTCGGCTTC AAGCCGATCG CGCGCCATAT CAGCAAGGAC GTGACGCTGT TCCGTCAGGC CGACATGAAT TTCCTGCTCA ATGCCGAGCC GGATTCGTTC GCCGAGCGTT ACGCGGAAGA ATATGGCGTG GGCATTTGCG CAATCGGCAT TCGCGTCGCC GATGCACAAC GCGCTTACGA TCGCGCCATC GAACTCGGCG CGTGGTCGTT CGAAGGCGAG CGCCTCGGCA AGGGCGAACT CGTGATCCCC GCCATTCAGG GCATCGGCGA CTCGCACATT TACTTCATCG ACCGCTGGCG CGGGCGCGGC GGCCAGAAGG GCGGCCTGGG CGACATCTCG ATCTTCGACA TCGACTTTCG TCCGATCGAA GTCAGCACCG CGCAAGCCGA TCTGAGCCAC GCGGGCACGG GTCTCGTCGC CGTCGATCAT CTGACGCAAA CGGTCGGCGC AGGCCGCATG CAGGAGTGGA TCGACTTCTA TCGCGAGCTG CTCAATTTCC GCGAGATTCA TGAACTGCAC GCGAATTGGC ATGTGTCGGC GGAATCGCGT GTGATGGTGT CGCCGTGCGG CGCGATCCGC ATTCCCCTCT ACGAGGAAGG CACGCGCCGC ACGAGCCTGA TGCACGAGTA TCTGCCCGAT CATCCCGGCG AAGGCGTGCA GCACATCGCG CTCGCGACCG ACGACATCTT CGCGTGCACC GATCAGCTGC TTGCCAACGG CATCGAACTG GTCGAGCCGC CGCCCGCGTA TTACGAGCAG ATCGACGCTC GCTTGCCGGG ACATGGGCTC GATATCGAGC GCCTGAAGCG CGGACGGATT CTCGTGGACG GCGAGATCGG CGCAGACGGC GTGCCGCTTC TGTTTTTCCA GACCTTTGTG CTGCGACGAG AAGGGGACAT CTTCTTCGAG ATCGTGCAGC GCGAGGGTCA TCACGGTTTC GGCGAGGGCA ATCTCAGCGC ACTGGCGCAG GCGCGCTCGG CCGCA
Details for BuphA.00134.a.B1.GE40203
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 42kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMP SDLPTPNDAL KAVADPEHNP LGTAGIEFVE FAARHPQMLA ETFVKLGFKP IARHISKDVT LFRQADMNFL LNAEPDSFAE RYAEEYGVGI CAIGIRVADA QRAYDRAIEL GAWSFEGERL GKGELVIPAI QGIGDSHIYF IDRWRGRGGQ KGGLGDISIF DIDFRPIEVS TAQADLSHAG TGLVAVDHLT QTVGAGRMQE WIDFYRELLN FREIHELHAN WHVSAESRVM VSPCGAIRIP LYEEGTRRTS LMHEYLPDHP GEGVQHIALA TDDIFACTDQ LLANGIELVE PPPAYYEQID ARLPGHGLDI ERLKRGRILV DGEIGADGVP LLFFQTFVLR REGDIFFEIV QREGHHGFGE GNL
Validated NT Sequence
gctgagattg ccctcgccga aaccgtgatg accctcgcgc tgcacgatct cgaagaagat gtccccttct cgtcgcagca caaaggtctg gaaaaacaga agcggcacgc cgtctgcgcc gatctcgccg tccacgagaa tccgtccgcg cttcaggcgc tcgatatcga gcccatgtcc cggcaagcga gcgtcgatct gctcgtaata cgcgggcggc ggctcgacca gttcgatgcc gttggcaagc agctgatcgg tgcacgcgaa gatgtcgtcg gtcgcgagcg cgatgtgctg cacgccttcg ccgggatgat cgggcagata ctcgtgcatc aggctcgtgc ggcgcgtgcc ttcctcgtag aggggaatgc ggatcgcgcc gcacggcgac accatcacac gcgattccgc cgacacatgc caattcgcgt gcagttcatg aatctcgcgg aaattgagca gctcgcgata gaagtcgatc cactcctgca tgcggcctgc gccgaccgtt tgcgtcagat gatcgacggc gacgagaccc gtgcccgcgt ggctcagatc ggcttgcgcg gtgctgactt cgatcggacg aaagtcgatg tcgaagatcg agatgtcgcc caggccgccc ttctggccgc cgcgcccgcg ccagcggtcg atgaagtaaa tgtgcgagtc gccgatgccc tgaatggcgg ggatcacgag ttcgcccttg ccgaggcgct cgccttcgaa cgaccacgcg ccgagttcga tggcgcgatc gtaagcgcgt tgtgcatcgg cgacgcgaat gccgattgcg caaatgccca cgccatattc ttccgcgtaa cgctcggcga acgaatccgg ctcggcattg agcaggaaat tcatgtcggc ctgacggaac agcgtcacgt ccttgctgat atggcgcgcg atcggcttga agccgagctt cacgaacgtc tccgccagca tctgcgggtg acgcgccgcg aattcgacga actcgatgcc ggctgtgccg agcgggttgt gctccgggtc cgcgacggcc ttgagcgcgt cgttgggggt cggcaggtcg ctgggcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMP SDLPTPNDAL KAVADPEHNP LGTAGIEFVE FAARHPQMLA ETFVKLGFKP IARHISKDVT LFRQADMNFL LNAEPDSFAE RYAEEYGVGI CAIGIRVADA QRAYDRAIEL GAWSFEGERL GKGELVIPAI QGIGDSHIYF IDRWRGRGGQ KGGLGDISIF DIDFRPIEVS TAQADLSHAG TGLVAVDHLT QTVGAGRMQE WIDFYRELLN FREIHELHAN WHVSAESRVM VSPCGAIRIP LYEEGTRRTS LMHEYLPDHP GEGVQHIALA TDDIFACTDQ LLANGIELVE PPPAYYEQID ARLPGHGLDI ERLKRGRILV DGEIGADGVP LLFFQTFVLR REGDIFFEIV QREGHHGFGE GNLSALAQAR SAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccca gcgacctgcc gacccccaac gacgcgctca aggccgtcgc ggacccggag cacaacccgc tcggcacagc cggcatcgag ttcgtcgaat tcgcggcgcg tcacccgcag atgctggcgg agacgttcgt gaagctcggc ttcaagccga tcgcgcgcca tatcagcaag gacgtgacgc tgttccgtca ggccgacatg aatttcctgc tcaatgccga gccggattcg ttcgccgagc gttacgcgga agaatatggc gtgggcattt gcgcaatcgg cattcgcgtc gccgatgcac aacgcgctta cgatcgcgcc atcgaactcg gcgcgtggtc gttcgaaggc gagcgcctcg gcaagggcga actcgtgatc cccgccattc agggcatcgg cgactcgcac atttacttca tcgaccgctg gcgcgggcgc ggcggccaga agggcggcct gggcgacatc tcgatcttcg acatcgactt tcgtccgatc gaagtcagca ccgcgcaagc cgatctgagc cacgcgggca cgggtctcgt cgccgtcgat catctgacgc aaacggtcgg cgcaggccgc atgcaggagt ggatcgactt ctatcgcgag ctgctcaatt tccgcgagat tcatgaactg cacgcgaatt ggcatgtgtc ggcggaatcg cgtgtgatgg tgtcgccgtg cggcgcgatc cgcattcccc tctacgagga aggcacgcgc cgcacgagcc tgatgcacga gtatctgccc gatcatcccg gcgaaggcgt gcagcacatc gcgctcgcga ccgacgacat cttcgcgtgc accgatcagc tgcttgccaa cggcatcgaa ctggtcgagc cgccgcccgc gtattacgag cagatcgacg ctcgcttgcc gggacatggg ctcgatatcg agcgcctgaa gcgcggacgg attctcgtgg acggcgagat cggcgcagac ggcgtgccgc ttctgttttt ccagaccttt gtgctgcgac gagaagggga catcttcttc gagatcgtgc agcgcgaggg tcatcacggt ttcggcgagg gcaatctcag cgcactggcg caggcgcgct cggccgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuphA.00134.a.B1.PS38139
PURIFICATION DATe: 12/7/2016
CONCENTRATION: 45mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMP SDLPTPNDAL KAVADPEHNP LGTAGIEFVE FAARHPQMLA ETFVKLGFKP IARHISKDVT LFRQADMNFL LNAEPDSFAE RYAEEYGVGI CAIGIRVADA QRAYDRAIEL GAWSFEGERL GKGELVIPAI QGIGDSHIYF IDRWRGRGGQ KGGLGDISIF DIDFRPIEVS TAQADLSHAG TGLVAVDHLT QTVGAGRMQE WIDFYRELLN FREIHELHAN WHVSAESRVM VSPCGAIRIP LYEEGTRRTS LMHEYLPDHP GEGVQHIALA TDDIFACTDQ LLANGIELVE PPPAYYEQID ARLPGHGLDI ERLKRGRILV DGEIGADGVP LLFFQTFVLR REGDIFFEIV QREGHHGFGE GNL
Validated NT Sequence
gctgagattg ccctcgccga aaccgtgatg accctcgcgc tgcacgatct cgaagaagat gtccccttct cgtcgcagca caaaggtctg gaaaaacaga agcggcacgc cgtctgcgcc gatctcgccg tccacgagaa tccgtccgcg cttcaggcgc tcgatatcga gcccatgtcc cggcaagcga gcgtcgatct gctcgtaata cgcgggcggc ggctcgacca gttcgatgcc gttggcaagc agctgatcgg tgcacgcgaa gatgtcgtcg gtcgcgagcg cgatgtgctg cacgccttcg ccgggatgat cgggcagata ctcgtgcatc aggctcgtgc ggcgcgtgcc ttcctcgtag aggggaatgc ggatcgcgcc gcacggcgac accatcacac gcgattccgc cgacacatgc caattcgcgt gcagttcatg aatctcgcgg aaattgagca gctcgcgata gaagtcgatc cactcctgca tgcggcctgc gccgaccgtt tgcgtcagat gatcgacggc gacgagaccc gtgcccgcgt ggctcagatc ggcttgcgcg gtgctgactt cgatcggacg aaagtcgatg tcgaagatcg agatgtcgcc caggccgccc ttctggccgc cgcgcccgcg ccagcggtcg atgaagtaaa tgtgcgagtc gccgatgccc tgaatggcgg ggatcacgag ttcgcccttg ccgaggcgct cgccttcgaa cgaccacgcg ccgagttcga tggcgcgatc gtaagcgcgt tgtgcatcgg cgacgcgaat gccgattgcg caaatgccca cgccatattc ttccgcgtaa cgctcggcga acgaatccgg ctcggcattg agcaggaaat tcatgtcggc ctgacggaac agcgtcacgt ccttgctgat atggcgcgcg atcggcttga agccgagctt cacgaacgtc tccgccagca tctgcgggtg acgcgccgcg aattcgacga actcgatgcc ggctgtgccg agcgggttgt gctccgggtc cgcgacggcc ttgagcgcgt cgttgggggt cggcaggtcg ctgggcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMP SDLPTPNDAL KAVADPEHNP LGTAGIEFVE FAARHPQMLA ETFVKLGFKP IARHISKDVT LFRQADMNFL LNAEPDSFAE RYAEEYGVGI CAIGIRVADA QRAYDRAIEL GAWSFEGERL GKGELVIPAI QGIGDSHIYF IDRWRGRGGQ KGGLGDISIF DIDFRPIEVS TAQADLSHAG TGLVAVDHLT QTVGAGRMQE WIDFYRELLN FREIHELHAN WHVSAESRVM VSPCGAIRIP LYEEGTRRTS LMHEYLPDHP GEGVQHIALA TDDIFACTDQ LLANGIELVE PPPAYYEQID ARLPGHGLDI ERLKRGRILV DGEIGADGVP LLFFQTFVLR REGDIFFEIV QREGHHGFGE GNLSALAQAR SAA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCCCA GCGACCTGCC GACCCCCAAC GACGCGCTCA AGGCCGTCGC GGACCCGGAG CACAACCCGC TCGGCACAGC CGGCATCGAG TTCGTCGAAT TCGCGGCGCG TCACCCGCAG ATGCTGGCGG AGACGTTCGT GAAGCTCGGC TTCAAGCCGA TCGCGCGCCA TATCAGCAAG GACGTGACGC TGTTCCGTCA GGCCGACATG AATTTCCTGC TCAATGCCGA GCCGGATTCG TTCGCCGAGC GTTACGCGGA AGAATATGGC GTGGGCATTT GCGCAATCGG CATTCGCGTC GCCGATGCAC AACGCGCTTA CGATCGCGCC ATCGAACTCG GCGCGTGGTC GTTCGAAGGC GAGCGCCTCG GCAAGGGCGA ACTCGTGATC CCCGCCATTC AGGGCATCGG CGACTCGCAC ATTTACTTCA TCGACCGCTG GCGCGGGCGC GGCGGCCAGA AGGGCGGCCT GGGCGACATC TCGATCTTCG ACATCGACTT TCGTCCGATC GAAGTCAGCA CCGCGCAAGC CGATCTGAGC CACGCGGGCA CGGGTCTCGT CGCCGTCGAT CATCTGACGC AAACGGTCGG CGCAGGCCGC ATGCAGGAGT GGATCGACTT CTATCGCGAG CTGCTCAATT TCCGCGAGAT TCATGAACTG CACGCGAATT GGCATGTGTC GGCGGAATCG CGTGTGATGG TGTCGCCGTG CGGCGCGATC CGCATTCCCC TCTACGAGGA AGGCACGCGC CGCACGAGCC TGATGCACGA GTATCTGCCC GATCATCCCG GCGAAGGCGT GCAGCACATC GCGCTCGCGA CCGACGACAT CTTCGCGTGC ACCGATCAGC TGCTTGCCAA CGGCATCGAA CTGGTCGAGC CGCCGCCCGC GTATTACGAG CAGATCGACG CTCGCTTGCC GGGACATGGG CTCGATATCG AGCGCCTGAA GCGCGGACGG ATTCTCGTGG ACGGCGAGAT CGGCGCAGAC GGCGTGCCGC TTCTGTTTTT CCAGACCTTT GTGCTGCGAC GAGAAGGGGA CATCTTCTTC GAGATCGTGC AGCGCGAGGG TCATCACGGT TTCGGCGAGG GCAATCTCAG CGCACTGGCG CAGGCGCGCT CGGCCGCATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT