BuphA.00133.a

Cysteinyl-tRNA synthetase

CENTER ID: BuphA.00133.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00133.a.B1.GE35966 Full length( BuphA.00133.a ) 1 465
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.7032
RefSeq: YP_001862763.1
UniProt: B2JT14

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPPLMLYDTW SRALRPFVSI EAGRAGMYCC GPTVYDHAHI GNLRTYVFED VLRRALVRNG YQLRHVVNIT DVGHLVSDAD EGEDKMEKGS RRTGESAWTI ASRYTDAFVS DWRALNLLEP SIWCRATDHI AEQIAFIVEL ERGGYVYRTD DGLYFDTGKQ HSYGYLARLD RAGLQAGKRV ALGAKKNITD FALWKFSPVD VKRQMEWDSP WGRGFPGWHI ECSAMSAKYL GTSFDIHCGG EDHIAVHHSN EIAQTEAAYG TRLANFWLHG RFLTLSAAKM SKSNGDFIRL QTLRDRHIDP IAYRYLCLTA HYRSNLHFTW ESLYAAQAAL NRLRHAYARW RQKNALADAR FVARFDAEVN EDLNMPNALA VLWDLVKSDL PDATRRASVD SFDAVLGLGL RDWTPLATEI PEDIQALLDE RARVRLEGDW AKSDALRTTL QLRGWQVEDG RDGQRVLPFS RNGAE
NT Sequence
ATGCCGCCGC TGATGCTGTA TGACACCTGG TCGCGCGCGC TGCGCCCGTT TGTATCGATC GAAGCGGGCC GTGCCGGAAT GTATTGCTGC GGCCCAACGG TCTACGATCA CGCGCACATC GGCAACCTTA GAACCTATGT TTTCGAAGAC GTCCTGCGTC GCGCGCTGGT GCGCAATGGC TACCAATTGC GTCACGTCGT GAACATCACC GATGTCGGTC ATCTGGTCTC CGATGCCGAC GAGGGCGAAG ACAAGATGGA AAAGGGCAGT CGACGGACGG GCGAGTCGGC ATGGACAATC GCGAGCCGTT ATACGGACGC TTTCGTTTCG GACTGGCGCG CGCTCAATCT GCTGGAGCCT TCAATCTGGT GCCGCGCGAC GGATCATATT GCCGAGCAGA TTGCTTTTAT CGTAGAACTC GAACGTGGGG GCTACGTATA CCGGACGGAC GACGGCCTCT ACTTCGATAC CGGCAAGCAG CACAGTTACG GCTATCTCGC GCGCCTGGAC CGTGCCGGTT TGCAGGCGGG CAAGCGCGTG GCGCTCGGCG CGAAGAAGAA CATCACGGAC TTCGCGTTGT GGAAGTTCAG TCCCGTCGAC GTCAAACGGC AGATGGAATG GGACAGCCCG TGGGGGCGGG GTTTTCCAGG TTGGCATATC GAGTGCTCGG CGATGTCCGC GAAGTACCTC GGCACGTCGT TCGATATCCA CTGCGGCGGC GAAGACCACA TCGCCGTGCA TCACAGCAAT GAAATTGCGC AGACGGAAGC GGCCTACGGT ACGCGCCTCG CGAATTTCTG GCTGCATGGG CGCTTCCTGA CTCTCAGCGC CGCGAAGATG TCGAAGTCGA ATGGAGATTT CATACGCTTG CAAACGCTGC GTGACAGACA CATCGATCCG ATTGCATACC GCTATCTGTG CCTCACCGCG CACTACCGCA GCAACTTGCA TTTCACCTGG GAATCGCTCT ACGCCGCGCA GGCAGCGTTG AACCGGCTAC GCCACGCGTA TGCCCGCTGG CGCCAAAAAA ACGCGCTCGC CGATGCGCGA TTTGTCGCGC GGTTCGATGC CGAAGTGAAC GAGGATCTCA ACATGCCTAA CGCGCTTGCG GTGCTCTGGG ATCTGGTAAA GAGCGACTTG CCGGATGCGA CGAGGCGCGC GAGCGTCGAC AGCTTCGATG CCGTGCTCGG ACTCGGCTTG CGCGATTGGA CACCGCTCGC GACAGAGATT CCCGAGGACA TTCAGGCCTT GCTCGACGAA CGCGCACGGG TACGGCTCGA AGGGGACTGG GCAAAGTCCG ATGCACTCAG AACGACATTA CAGCTGCGAG GGTGGCAGGT TGAAGACGGG CGCGATGGTC AACGTGTGCT TCCGTTTTCC AGAAATGGCG CGGAA
Details for BuphA.00133.a.B1.GE35966
HARVESTED ON: 8/28/2012
SEQUENCED ON: 8/31/2012
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 82
PERCENT COVERAGE: 81
Validated AA Sequence
MAHHHHHHMP PLMLYDTWSR ALRPFVSIEA GRAGMYCCGP TVYDHAHIGN LRTYVFEDVL RRALVRNGYQ LRHVVNITDV GHLVSDADEG EDKMEKGSRR TGESAWTIAS RYTDAFVSDW RALNLLEPSI WCRATDHIAE QIAFIVELER GGYVYRTDDG LYFDTGKQHS YGYLARLDRA GLQAGKRVAL GAKKNITDFA LWKFSPVDVK RQMEWDSPWG RGFPGWHIEC SAMSAKYLGT SFDIHCGGED HIAVHHSNEI AQTEAAYGTR LANFWLHGRF LTLSAAKMSK SNGDFIRLQT LRDRXIDPIA YRYLCXXXXP QXLAFHLXXA LRXXXXXXXX XXCXXXXKXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXX
Validated NT Sequence
ttgtttnnct ttaagaagga gatataccat ggctcaccac caccaccacc atatgccgcc gctgatgctg tatgacacct ggtcgcgcgc gctgcgcccg tttgtatcga tcgaagcggg ccgtgccgga atgtattgct gcggcccaac ggtctacgat cacgcgcaca tcggcaacct tagaacctat gttttcgaag acgtcctgcg tcgcgcgctg gtgcgcaatg gctaccaatt gcgtcacgtc gtgaacatca ccgatgtcgg tcatctggtc tccgatgccg acgagggcga agacaagatg gaaaagggca gtcgacggac gggcgagtcg gcatggacaa tcgcgagccg ttatacggac gctttcgttt cggactggcg cgcgctcaat ctgctggagc cttcaatctg gtgccgcgcg acggatcata ttgccgagca gattgctttt atcgtagaac tcgaacgtgg gggctacgta taccggacgg acgacggcct ctacttcgat accggcaagc agcacagtta cggctatctc gcgcgcctgg accgtgccgg tttgcaggcg ggcaagcgcg tggcgctcgg cgcgaagaag aacatcacgg acttcgcgtt gtggaagttc agtcccgtcg acgtcaaacg gcagatggaa tgggacagcc cgtgggggcg gggttttcca ggttggcata tcgagtgctc ggcgatgtcc gcgaagtacc tcggcacgtc gttcgatatc cactgcggcg gcgaagacca catcgccgtg catcacagca atgaaattgc gcagacggaa gcggcctacg gtacgcgcct cgcgaatttc tggctgcatg ggcgcttcct gactctcagc gccgcgaaga tgtcgaagtc naatggagat ttcatacgct tgcaaacgct gcgtgacaga cncatcgatc cgattgcata ccgctatctg tgcntcancn cnntaccgca gcancttgca tttcacctgg nnnnngctct acgncnnnnn nnnttnnnnn nnnacnnnnn nntatgccnn ntgncgncaa aaannncnnn nnangnnnna ttnnnnnnnn nnnnnnnnnn nannnnnnnn nnnnnnnnnn nnngnnnnnn nnnnnnnnnn nnnnnntnnn nnnnnnnnnn nnnnnnnnnn cntt
Expected Protein Sequence
MAHHHHHHMP PLMLYDTWSR ALRPFVSIEA GRAGMYCCGP TVYDHAHIGN LRTYVFEDVL RRALVRNGYQ LRHVVNITDV GHLVSDADEG EDKMEKGSRR TGESAWTIAS RYTDAFVSDW RALNLLEPSI WCRATDHIAE QIAFIVELER GGYVYRTDDG LYFDTGKQHS YGYLARLDRA GLQAGKRVAL GAKKNITDFA LWKFSPVDVK RQMEWDSPWG RGFPGWHIEC SAMSAKYLGT SFDIHCGGED HIAVHHSNEI AQTEAAYGTR LANFWLHGRF LTLSAAKMSK SNGDFIRLQT LRDRHIDPIA YRYLCLTAHY RSNLHFTWES LYAAQAALNR LRHAYARWRQ KNALADARFV ARFDAEVNED LNMPNALAVL WDLVKSDLPD ATRRASVDSF DAVLGLGLRD WTPLATEIPE DIQALLDERA RVRLEGDWAK SDALRTTLQL RGWQVEDGRD GQRVLPFSRN GAE
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgccgc cgctgatgct gtatgacacc tggtcgcgcg cgctgcgccc gtttgtatcg atcgaagcgg gccgtgccgg aatgtattgc tgcggcccaa cggtctacga tcacgcgcac atcggcaacc ttagaaccta tgttttcgaa gacgtcctgc gtcgcgcgct ggtgcgcaat ggctaccaat tgcgtcacgt cgtgaacatc accgatgtcg gtcatctggt ctccgatgcc gacgagggcg aagacaagat ggaaaagggc agtcgacgga cgggcgagtc ggcatggaca atcgcgagcc gttatacgga cgctttcgtt tcggactggc gcgcgctcaa tctgctggag ccttcaatct ggtgccgcgc gacggatcat attgccgagc agattgcttt tatcgtagaa ctcgaacgtg ggggctacgt ataccggacg gacgacggcc tctacttcga taccggcaag cagcacagtt acggctatct cgcgcgcctg gaccgtgccg gtttgcaggc gggcaagcgc gtggcgctcg gcgcgaagaa gaacatcacg gacttcgcgt tgtggaagtt cagtcccgtc gacgtcaaac ggcagatgga atgggacagc ccgtgggggc ggggttttcc aggttggcat atcgagtgct cggcgatgtc cgcgaagtac ctcggcacgt cgttcgatat ccactgcggc ggcgaagacc acatcgccgt gcatcacagc aatgaaattg cgcagacgga agcggcctac ggtacgcgcc tcgcgaattt ctggctgcat gggcgcttcc tgactctcag cgccgcgaag atgtcgaagt cgaatggaga tttcatacgc ttgcaaacgc tgcgtgacag acacatcgat ccgattgcat accgctatct gtgcctcacc gcgcactacc gcagcaactt gcatttcacc tgggaatcgc tctacgccgc gcaggcagcg ttgaaccggc tacgccacgc gtatgcccgc tggcgccaaa aaaacgcgct cgccgatgcg cgatttgtcg cgcggttcga tgccgaagtg aacgaggatc tcaacatgcc taacgcgctt gcggtgctct gggatctggt aaagagcgac ttgccggatg cgacgaggcg cgcgagcgtc gacagcttcg atgccgtgct cggactcggc ttgcgcgatt ggacaccgct cgcgacagag attcccgagg acattcaggc cttgctcgac gaacgcgcac gggtacggct cgaaggggac tgggcaaagt ccgatgcact cagaacgaca ttacagctgc gagggtggca ggttgaagac gggcgcgatg gtcaacgtgt gcttccgttt tccagaaatg gcgcggaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat