BuphA.00120.a

GTPase obg

CENTER ID: BuphA.00120.a
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00120.a.A1.GE32280 Full length( BuphA.00120.a ) 1 370
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00120.a.A1.PS01255 Full length( BuphA.00120.a ) 1 370

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.2811
RefSeq: YP_001858868.1
UniProt: B2JHD7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGSVYAVAD RNINTLIDYR YSKKHQARNG ENGRGSDCYG KGGDDITLRM PVGTIITDMD TGELIADLTE HNQTVRIAEG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MVRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSRSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGL LLHIVDLAPF DETVDPVAEA KAIVNELRKY DEELFSKPRW LVLNKLDMVP EDEREARVAA FLKDFEWDGP VFEISALTGQ GCENLCYAVY DYLAEHSDAQ RAAEAEDLAA DVRFRGQQQG DANAPVGPQE
NT Sequence
ATGAAGTTCA TTGACGAAGC GAGGATCGAA GTCATCGCCG GCGACGGAGG AGATGGCAGC GCGTCGATGC GCCGCGAGAA ATTCGTCCCG TTCGGCGGTC CGGACGGCGG CGACGGCGGC CGTGGCGGCA GCGTCTATGC AGTCGCAGAT CGCAATATCA ACACGCTGAT CGATTACCGC TACTCGAAAA AGCATCAGGC TCGCAACGGC GAAAACGGTC GTGGCTCGGA CTGCTATGGC AAGGGCGGCG ACGACATCAC GCTGCGCATG CCTGTCGGCA CCATCATCAC CGACATGGAC ACGGGCGAGC TGATCGCCGA CCTCACCGAG CACAATCAGA CGGTGCGCAT CGCCGAAGGC GGCGCGGGTG GGCTCGGCAA TCTGCATTTC AAATCCAGTA CGAATCGCGC GCCGCGACAG AAGACGGACG GCAAGCCCGG CGAACGCCGA ATGGTGCGCC TCGAGTTGAA GGTGCTCGCG GATGTCGGTT TGCTCGGCAT GCCGAACGCA GGCAAGTCGA CGTTCATTTC GTCGGTGTCG AACGCGAAGC CGAAGATCGC CGACTATCCA TTCACGACGC TCGCGCCCAA TCTCGGCGTG GTGCGTGTCG GGCCGAGCCG CAGCTTCGTG ATCGCGGATA TTCCCGGGCT GATCGAAGGT GCGGCGGAAG GCGCGGGCCT CGGTCACCAG TTCCTGCGCC ATCTGCAGCG CACGGGCCTG TTGCTTCACA TCGTCGATCT CGCACCGTTC GACGAAACCG TCGATCCCGT CGCGGAAGCC AAGGCGATCG TCAACGAACT GCGCAAGTAC GACGAAGAGC TGTTCAGCAA GCCGCGCTGG CTCGTGTTGA ACAAGCTCGA CATGGTGCCA GAAGACGAGC GGGAGGCACG CGTGGCCGCG TTCCTGAAGG ACTTCGAATG GGACGGCCCG GTGTTCGAAA TCTCGGCGCT CACCGGTCAG GGCTGCGAGA ACCTGTGCTA CGCGGTGTAC GACTACCTTG CCGAGCACTC GGATGCGCAG CGCGCGGCAG AAGCCGAAGA TCTGGCCGCC GACGTGCGTT TTCGCGGGCA GCAGCAAGGT GACGCGAACG CGCCCGTCGG GCCGCAGGAG
Details for BuphA.00120.a.A1.GE32280
HARVESTED ON: 7/28/2011
SEQUENCED ON: 8/3/2011
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGSVYAVAD RNINTLIDYR YSKKHQARNG ENGRGSDCYG KGGDDITLRM PVGTIITDMD TGELIADLTE HNQTVRIAEG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MVRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSRSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGL LLHIVDLAPF DETVDPVAEA KAIVNELRKY DEELFSKPRW LVLNKLDMVP EDEREARVAA FLKDFEWDGP VFEISALTGQ GCENLCYAVY DYLAEHSDAQ RAAEAEDLAA DVRFRGQQQG DANAPVG
Validated NT Sequence
cggcccgacg ggcgcgttcg cgtcaccttg ctgctgcccg cgaaaacgca cgtcggcggc cagatcttcg gcttctgccg cgcgctgcgc atccgagtgc tcggcaaggt agtcgtacac cgcgtagcac aggttctcgc agccctgacc ggtgagcgcc gagatttcga acaccgggcc gtcccattcg aagtccttca ggaacgcggc cacgcgtgcc tcccgctcgt cttctggcac catgtcgagc ttgttcaaca cgagccagcg cggcttgctg aacagctctt cgtcgtactt gcgcagttcg ttgacgatcg ccttggcttc cgcgacggga tcgacggttt cgtcgaacgg tgcgagatcg acgatgtgaa gcaacaggcc cgtgcgctgc agatggcgca ggaactggtg accgaggccc gcgccttccg ccgcaccttc gatcagcccg ggaatatccg cgatcacgaa gctgcggctc ggcccgacac gcaccacgcc gagattgggc gcgagcgtcg tgaatggata gtcggcgatc ttcggcttcg cgttcgacac cgacgaaatg aacgtcgact tgcctgcgtt cggcatgccg agcaaaccga catccgcgag caccttcaac tcgaggcgca ccattcggcg ttcgccgggc ttgccgtccg tcttctgtcg cggcgcgcga ttcgtactgg atttgaaatg cagattgccg agcccacccg cgccgccttc ggcgatgcgc accgtctgat tgtgctcggt gaggtcggcg atcagctcgc ccgtgtccat gtcggtgatg atggtgccga caggcatgcg cagcgtgatg tcgtcgccgc ccttgccata gcagtccgag ccacgaccgt tttcgccgtt gcgagcctga tgctttttcg agtagcggta atcgatcagc gtgttgatat tgcgatctgc gactgcatag acgctgccgc cacggccgcc gtcgccgccg tccggaccgc cgaacgggac gaatttctcg cggcgcatcg acgcgctgcc atctcctccg tcgccggcga tgacttcgat cctcgcttcg tcaatgaact tcatcgaacc aggaccctgg gtctgagctt ccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGSVYAVA DRNINTLIDY RYSKKHQARN GENGRGSDCY GKGGDDITLR MPVGTIITDM DTGELIADLT EHNQTVRIAE GGAGGLGNLH FKSSTNRAPR QKTDGKPGER RMVRLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPSRSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG LLLHIVDLAP FDETVDPVAE AKAIVNELRK YDEELFSKPR WLVLNKLDMV PEDEREARVA AFLKDFEWDG PVFEISALTG QGCENLCYAV YDYLAEHSDA QRAAEAEDLA ADVRFRGQQQ GDANAPVGPQ E
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagttcatt gacgaagcga ggatcgaagt catcgccggc gacggaggag atggcagcgc gtcgatgcgc cgcgagaaat tcgtcccgtt cggcggtccg gacggcggcg acggcggccg tggcggcagc gtctatgcag tcgcagatcg caatatcaac acgctgatcg attaccgcta ctcgaaaaag catcaggctc gcaacggcga aaacggtcgt ggctcggact gctatggcaa gggcggcgac gacatcacgc tgcgcatgcc tgtcggcacc atcatcaccg acatggacac gggcgagctg atcgccgacc tcaccgagca caatcagacg gtgcgcatcg ccgaaggcgg cgcgggtggg ctcggcaatc tgcatttcaa atccagtacg aatcgcgcgc cgcgacagaa gacggacggc aagcccggcg aacgccgaat ggtgcgcctc gagttgaagg tgctcgcgga tgtcggtttg ctcggcatgc cgaacgcagg caagtcgacg ttcatttcgt cggtgtcgaa cgcgaagccg aagatcgccg actatccatt cacgacgctc gcgcccaatc tcggcgtggt gcgtgtcggg ccgagccgca gcttcgtgat cgcggatatt cccgggctga tcgaaggtgc ggcggaaggc gcgggcctcg gtcaccagtt cctgcgccat ctgcagcgca cgggcctgtt gcttcacatc gtcgatctcg caccgttcga cgaaaccgtc gatcccgtcg cggaagccaa ggcgatcgtc aacgaactgc gcaagtacga cgaagagctg ttcagcaagc cgcgctggct cgtgttgaac aagctcgaca tggtgccaga agacgagcgg gaggcacgcg tggccgcgtt cctgaaggac ttcgaatggg acggcccggt gttcgaaatc tcggcgctca ccggtcaggg ctgcgagaac ctgtgctacg cggtgtacga ctaccttgcc gagcactcgg atgcgcagcg cgcggcagaa gccgaagatc tggccgccga cgtgcgtttt cgcgggcagc agcaaggtga cgcgaacgcg cccgtcgggc cgcaggagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for BuphA.00120.a.A1.PS01255
PURIFICATION DATe: 9/28/2011
CONCENTRATION: 62.99mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MKFIDEARIE VIAGDGGDGS ASMRREKFVP FGGPDGGDGG RGGSVYAVAD RNINTLIDYR YSKKHQARNG ENGRGSDCYG KGGDDITLRM PVGTIITDMD TGELIADLTE HNQTVRIAEG GAGGLGNLHF KSSTNRAPRQ KTDGKPGERR MVRLELKVLA DVGLLGMPNA GKSTFISSVS NAKPKIADYP FTTLAPNLGV VRVGPSRSFV IADIPGLIEG AAEGAGLGHQ FLRHLQRTGL LLHIVDLAPF DETVDPVAEA KAIVNELRKY DEELFSKPRW LVLNKLDMVP EDEREARVAA FLKDFEWDGP VFEISALTGQ GCENLCYAVY DYLAEHSDAQ RAAEAEDLAA DVRFRGQQQG DANAPVG
Validated NT Sequence
cggcccgacg ggcgcgttcg cgtcaccttg ctgctgcccg cgaaaacgca cgtcggcggc cagatcttcg gcttctgccg cgcgctgcgc atccgagtgc tcggcaaggt agtcgtacac cgcgtagcac aggttctcgc agccctgacc ggtgagcgcc gagatttcga acaccgggcc gtcccattcg aagtccttca ggaacgcggc cacgcgtgcc tcccgctcgt cttctggcac catgtcgagc ttgttcaaca cgagccagcg cggcttgctg aacagctctt cgtcgtactt gcgcagttcg ttgacgatcg ccttggcttc cgcgacggga tcgacggttt cgtcgaacgg tgcgagatcg acgatgtgaa gcaacaggcc cgtgcgctgc agatggcgca ggaactggtg accgaggccc gcgccttccg ccgcaccttc gatcagcccg ggaatatccg cgatcacgaa gctgcggctc ggcccgacac gcaccacgcc gagattgggc gcgagcgtcg tgaatggata gtcggcgatc ttcggcttcg cgttcgacac cgacgaaatg aacgtcgact tgcctgcgtt cggcatgccg agcaaaccga catccgcgag caccttcaac tcgaggcgca ccattcggcg ttcgccgggc ttgccgtccg tcttctgtcg cggcgcgcga ttcgtactgg atttgaaatg cagattgccg agcccacccg cgccgccttc ggcgatgcgc accgtctgat tgtgctcggt gaggtcggcg atcagctcgc ccgtgtccat gtcggtgatg atggtgccga caggcatgcg cagcgtgatg tcgtcgccgc ccttgccata gcagtccgag ccacgaccgt tttcgccgtt gcgagcctga tgctttttcg agtagcggta atcgatcagc gtgttgatat tgcgatctgc gactgcatag acgctgccgc cacggccgcc gtcgccgccg tccggaccgc cgaacgggac gaatttctcg cggcgcatcg acgcgctgcc atctcctccg tcgccggcga tgacttcgat cctcgcttcg tcaatgaact tcatcgaacc aggaccctgg gtctgagctt ccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKFIDEARI EVIAGDGGDG SASMRREKFV PFGGPDGGDG GRGGSVYAVA DRNINTLIDY RYSKKHQARN GENGRGSDCY GKGGDDITLR MPVGTIITDM DTGELIADLT EHNQTVRIAE GGAGGLGNLH FKSSTNRAPR QKTDGKPGER RMVRLELKVL ADVGLLGMPN AGKSTFISSV SNAKPKIADY PFTTLAPNLG VVRVGPSRSF VIADIPGLIE GAAEGAGLGH QFLRHLQRTG LLLHIVDLAP FDETVDPVAE AKAIVNELRK YDEELFSKPR WLVLNKLDMV PEDEREARVA AFLKDFEWDG PVFEISALTG QGCENLCYAV YDYLAEHSDA QRAAEAEDLA ADVRFRGQQQ GDANAPVGPQ E
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAAGTTCATT GACGAAGCGA GGATCGAAGT CATCGCCGGC GACGGAGGAG ATGGCAGCGC GTCGATGCGC CGCGAGAAAT TCGTCCCGTT CGGCGGTCCG GACGGCGGCG ACGGCGGCCG TGGCGGCAGC GTCTATGCAG TCGCAGATCG CAATATCAAC ACGCTGATCG ATTACCGCTA CTCGAAAAAG CATCAGGCTC GCAACGGCGA AAACGGTCGT GGCTCGGACT GCTATGGCAA GGGCGGCGAC GACATCACGC TGCGCATGCC TGTCGGCACC ATCATCACCG ACATGGACAC GGGCGAGCTG ATCGCCGACC TCACCGAGCA CAATCAGACG GTGCGCATCG CCGAAGGCGG CGCGGGTGGG CTCGGCAATC TGCATTTCAA ATCCAGTACG AATCGCGCGC CGCGACAGAA GACGGACGGC AAGCCCGGCG AACGCCGAAT GGTGCGCCTC GAGTTGAAGG TGCTCGCGGA TGTCGGTTTG CTCGGCATGC CGAACGCAGG CAAGTCGACG TTCATTTCGT CGGTGTCGAA CGCGAAGCCG AAGATCGCCG ACTATCCATT CACGACGCTC GCGCCCAATC TCGGCGTGGT GCGTGTCGGG CCGAGCCGCA GCTTCGTGAT CGCGGATATT CCCGGGCTGA TCGAAGGTGC GGCGGAAGGC GCGGGCCTCG GTCACCAGTT CCTGCGCCAT CTGCAGCGCA CGGGCCTGTT GCTTCACATC GTCGATCTCG CACCGTTCGA CGAAACCGTC GATCCCGTCG CGGAAGCCAA GGCGATCGTC AACGAACTGC GCAAGTACGA CGAAGAGCTG TTCAGCAAGC CGCGCTGGCT CGTGTTGAAC AAGCTCGACA TGGTGCCAGA AGACGAGCGG GAGGCACGCG TGGCCGCGTT CCTGAAGGAC TTCGAATGGG ACGGCCCGGT GTTCGAAATC TCGGCGCTCA CCGGTCAGGG CTGCGAGAAC CTGTGCTACG CGGTGTACGA CTACCTTGCC GAGCACTCGG ATGCGCAGCG CGCGGCAGAA GCCGAAGATC TGGCCGCCGA CGTGCGTTTT CGCGGGCAGC AGCAAGGTGA CGCGAACGCG CCCGTCGGGC CGCAGGAGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A