SSGCID: Target Page

BuphA.00119.b

D-alanine--D-alanine ligase

CENTER ID:	BuphA.00119.b
ORGANISM:	Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuphA.00119.b.B1.GE35965	Full length( BuphA.00119.b )		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|391038.7.peg.7900
RefSeq:	YP_001863603.1
UniProt:	B2JY45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSRARISIA FGGKSTEHRA SLESFRHVIG EIHNDDSVEI EDIYYVDVEG RVAVTPFQAG LPVDSYVRDR EYMPYWAALG RMVNAGTFVL NLLYGNFGED GKIQGAAAIA SVPGSFGPVL AASLAMSKYH MNQYIASAHP VVQIPDTLLL RECDLPEAST RIFDRFHGME IVVKPNSLGA SLFTNRYLVD PECIESIKTN LAEIFEYDAN ALVQRYVSGT EYSIGCLQKN ETVMVLPAVR IETEGAFFGH EEKHRSGKAN EIIIMEESPV VKSLGDLSIE IFKSVDFRNM CRFDFIVSPE EAIFFLEANT IPGLMRNSIF PKMLAASDIT LGEAISQFHN NFLSERARKS FVDYHID

NT Sequence

ATGACGAGTC GCGCACGAAT TTCGATTGCA TTTGGAGGAA AATCAACTGA GCACCGGGCA TCGCTCGAAT CATTCCGCCA TGTTATCGGC GAGATTCACA ACGATGATTC CGTTGAGATC GAGGACATCT ACTATGTGGA TGTCGAAGGT CGCGTCGCCG TCACTCCGTT CCAAGCGGGC CTTCCAGTGG ACAGCTACGT GCGGGATCGC GAGTACATGC CATATTGGGC TGCCCTCGGA AGAATGGTAA ATGCCGGCAC GTTTGTTCTG AATCTGCTAT ATGGAAACTT CGGTGAAGAC GGTAAAATTC AAGGTGCGGC GGCAATAGCT TCTGTGCCAG GAAGTTTCGG CCCTGTCCTC GCGGCGAGTT TGGCGATGAG CAAGTATCAT ATGAATCAAT ATATAGCCAG CGCTCATCCA GTGGTGCAGA TACCCGACAC CCTCCTGTTG CGCGAGTGCG ACCTACCGGA AGCTAGTACC CGAATCTTCG ACCGATTCCA CGGAATGGAA ATCGTCGTCA AGCCGAATTC ATTAGGCGCC TCGCTTTTCA CAAATCGCTA TCTTGTCGAT CCGGAATGTA TAGAATCTAT TAAAACCAAT CTCGCGGAAA TATTTGAGTA TGACGCAAAT GCGCTGGTTC AGAGGTATGT CTCTGGCACT GAATACTCAA TAGGCTGTTT GCAGAAAAAT GAAACCGTCA TGGTCTTGCC TGCCGTCCGT ATAGAGACAG AGGGCGCATT TTTCGGACAT GAGGAAAAGC ACCGTTCTGG TAAGGCAAAT GAGATCATTA TCATGGAGGA ATCGCCAGTC GTCAAATCAC TCGGTGATCT ATCTATTGAG ATATTTAAAT CTGTCGATTT TAGAAACATG TGTAGATTCG ATTTTATAGT TTCTCCGGAG GAAGCGATCT TCTTCCTAGA GGCTAATACC ATCCCAGGGT TGATGCGCAA CAGCATCTTT CCAAAGATGC TTGCGGCGTC CGATATCACT CTCGGCGAGG CGATTTCGCA GTTCCACAAC AACTTTCTTT CCGAAAGAGC GCGCAAAAGC TTTGTGGACT ACCATATCGA C

Details for BuphA.00119.b.B1.GE35965

HARVESTED ON:	8/28/2012
SEQUENCED ON:	8/31/2012
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	50

Validated AA Sequence

Validated NT Sequence

attttgtnnn ntttaagaag gaganatacc atggctcacc accaccacca ccatatgacg agtcgcgcac gaatttcgat tgcatttgga ggaaaatcaa ctgagcaccg ggcatcgctc gaatcattcc gccatgttat cggcgagatt cacaacgatg attccgttga gatcgaggac atctactatg tggatgtcga aggtcgcgtc gccgtcactc cgttccaagc gggccttcca gtggacagct acgtgcggga tcgcgagtac atgccatatt gggctgccct cggaagaatg gtaaatgccg gcacgtttgt tctgaatctg ctatatggaa acttcggtga agacggtaaa attcaaggtg cggcggcaat agcttctgtg ccaggaagtt tcggccctgt cctcgcggcg agtttggcga tgagcaagta tcatatgaat caatatatag ccagcgctca tccagtggtg cagatacccg acaccctcct gttgcgcgag tgcgacctac cggaagctag tacccgaatc ttcgaccgat tccacggaat ggnaatcgtc gtcaagccna atcattagnc gcctcgcttt tcncaaatcg ctatcntgtc natccggaan gnatagaann nnntnaaanc nnnnnnnnnn nannatttnn nnangacnnn aatgnnntnn nncnnnngnn nnnnnnnngg nnntgantnn nnnaaannnn nnncangnnn nnnnnnnncn nnnnnnnnnn nnnggcnttt nnnnnnnnng naaannnntn ngnnngnnnn nnnnntnnnn nggannnnnn nnnnntnngt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntnt

Expected Protein Sequence

MAHHHHHHMT SRARISIAFG GKSTEHRASL ESFRHVIGEI HNDDSVEIED IYYVDVEGRV AVTPFQAGLP VDSYVRDREY MPYWAALGRM VNAGTFVLNL LYGNFGEDGK IQGAAAIASV PGSFGPVLAA SLAMSKYHMN QYIASAHPVV QIPDTLLLRE CDLPEASTRI FDRFHGMEIV VKPNSLGASL FTNRYLVDPE CIESIKTNLA EIFEYDANAL VQRYVSGTEY SIGCLQKNET VMVLPAVRIE TEGAFFGHEE KHRSGKANEI IIMEESPVVK SLGDLSIEIF KSVDFRNMCR FDFIVSPEEA IFFLEANTIP GLMRNSIFPK MLAASDITLG EAISQFHNNF LSERARKSFV DYHID

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga gtcgcgcacg aatttcgatt gcatttggag gaaaatcaac tgagcaccgg gcatcgctcg aatcattccg ccatgttatc ggcgagattc acaacgatga ttccgttgag atcgaggaca tctactatgt ggatgtcgaa ggtcgcgtcg ccgtcactcc gttccaagcg ggccttccag tggacagcta cgtgcgggat cgcgagtaca tgccatattg ggctgccctc ggaagaatgg taaatgccgg cacgtttgtt ctgaatctgc tatatggaaa cttcggtgaa gacggtaaaa ttcaaggtgc ggcggcaata gcttctgtgc caggaagttt cggccctgtc ctcgcggcga gtttggcgat gagcaagtat catatgaatc aatatatagc cagcgctcat ccagtggtgc agatacccga caccctcctg ttgcgcgagt gcgacctacc ggaagctagt acccgaatct tcgaccgatt ccacggaatg gaaatcgtcg tcaagccgaa ttcattaggc gcctcgcttt tcacaaatcg ctatcttgtc gatccggaat gtatagaatc tattaaaacc aatctcgcgg aaatatttga gtatgacgca aatgcgctgg ttcagaggta tgtctctggc actgaatact caataggctg tttgcagaaa aatgaaaccg tcatggtctt gcctgccgtc cgtatagaga cagagggcgc atttttcgga catgaggaaa agcaccgttc tggtaaggca aatgagatca ttatcatgga ggaatcgcca gtcgtcaaat cactcggtga tctatctatt gagatattta aatctgtcga ttttagaaac atgtgtagat tcgattttat agtttctccg gaggaagcga tcttcttcct agaggctaat accatcccag ggttgatgcg caacagcatc tttccaaaga tgcttgcggc gtccgatatc actctcggcg aggcgatttc gcagttccac aacaactttc tttccgaaag agcgcgcaaa agctttgtgg actaccatat cgactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat