BuphA.00118.c

Glucose-1-phosphate thymidylyltransferase

CENTER ID: BuphA.00118.c
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00118.c.B1.GE40199 Full length( BuphA.00118.c ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.7066
RefSeq: YP_001862795.1
UniProt: B2JT46

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MARKGIILAG GSGTRLYPVT HSVSKQLLPV YDKPMIYYPL CTLMVAGIRD VLVISTVEDT PRFQALLGDG SKWGINLQYA LQPSPDGLAQ AFIIGRSFVG NDNSALVLGD NIFYGHELVK QLAHANDRDD GATVFAYHVN DPERYGVVEF DPEFKALSIE EKPAKPRSSY AVTGLYFYDR QVCDIAADIK PSARGELEIT DVNVHYLRRQ QLHVEIMGRG YAWLDTGTHD SLIDASTFIA TLQKRQGMMV ACPEEIAFRM QWIDGDQLQA LARPLSKSAY GRYLTDIAQD RVAWPTREAT VEAEPQHIGS GRIRRVS
NT Sequence
ATGGCTCGTA AAGGAATCAT CCTCGCTGGC GGGTCGGGTA CGCGTCTGTA TCCGGTCACG CATTCGGTTT CGAAGCAGCT ACTTCCCGTC TACGACAAGC CGATGATTTA CTACCCGCTT TGCACGCTGA TGGTAGCGGG CATACGCGAT GTGCTCGTCA TATCGACGGT GGAAGATACA CCGCGGTTTC AGGCGCTTCT CGGCGACGGC AGCAAGTGGG GCATCAATCT GCAATATGCG CTGCAGCCAT CGCCGGACGG ACTGGCCCAG GCGTTCATCA TCGGCCGCTC GTTCGTCGGA AACGACAACT CTGCACTGGT TCTTGGGGAC AATATCTTTT ACGGGCACGA ACTGGTGAAG CAGCTGGCGC ACGCAAACGA CCGCGACGAC GGTGCGACGG TATTTGCCTA TCATGTGAAC GATCCGGAAC GGTATGGCGT TGTCGAATTC GACCCGGAAT TCAAGGCGCT TTCAATCGAA GAGAAGCCGG CGAAGCCGCG TTCGAGCTAC GCCGTTACCG GGCTCTACTT CTACGACCGA CAGGTCTGCG ACATTGCGGC GGACATCAAG CCTTCGGCAC GCGGCGAGCT CGAAATCACG GACGTCAACG TACATTATCT GCGGCGTCAG CAATTGCATG TCGAGATCAT GGGACGGGGT TACGCGTGGC TGGATACGGG CACCCATGAC TCGTTGATCG ACGCGTCCAC CTTTATCGCA ACGCTTCAGA AGCGGCAGGG CATGATGGTC GCTTGCCCGG AAGAAATCGC GTTTCGCATG CAATGGATCG ACGGCGACCA GTTGCAGGCC CTCGCCCGAC CGCTGTCGAA GTCGGCTTAC GGCCGGTACC TGACGGACAT TGCGCAGGAC CGGGTCGCAT GGCCGACCAG GGAGGCCACT GTAGAGGCAG AACCTCAACA TATTGGTAGC GGCCGCATTC GGCGCGTTTC T
Details for BuphA.00118.c.B1.GE40199
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMA RKGIILAGGS GTRLYPVTHS VSKQLLPVYD KPMIYYPLCT LMVAGIRDVL VISTVEDTPR FQALLGDGSK WGINLQYALQ PSPDGLAQAF IIGRSFVGND NSALVLGDNI FYGHELVKQL AHANDRDDGA TVFAYHVNDP ERYGVVEFDP EFKALSIEEK PAKPRSSYAV TGLYFYDRQV CDIAADIKPS ARGELEITDV NVHYLRRQQL HVEIMGRGYA WLDTGTHDSL IDASTFIATL QKRQGMMVAC PEEIAFRMQW IDGDQLQALA RPLSKSAYGR YLTDIAQDRV AWPTREATVE AEPQH
Validated NT Sequence
atatgttgag gttctgcctc tacagtggcc tccctggtcg gccatgcgac ccggtcctgc gcaatgtccg tcaggtaccg gccgtaagcc gacttcgaca gcggtcgggc gagggcctgc aactggtcgc cgtcgatcca ttgcatgcga aacgcgattt cttccgggca agcgaccatc atgccctgcc gcttctgaag cgttgcgata aaggtggacg cgtcgatcaa cgagtcatgg gtgcccgtat ccagccacgc gtaaccccgt cccatgatct cgacatgcaa ttgctgacgc cgcagataat gtacgttgac gtccgtgatt tcgagctcgc cgcgtgccga aggcttgatg tccgccgcaa tgtcgcagac ctgtcggtcg tagaagtaga gcccggtaac ggcgtagctc gaacgcggct tcgccggctt ctcttcgatt gaaagcgcct tgaattccgg gtcgaattcg acaacgccat accgttccgg atcgttcaca tgataggcaa ataccgtcgc accgtcgtcg cggtcgtttg cgtgcgccag ctgcttcacc agttcgtgcc cgtaaaagat attgtcccca agaaccagtg cagagttgtc gtttccgacg aacgagcggc cgatgatgaa cgcctgggcc agtccgtccg gcgatggctg cagcgcatat tgcagattga tgccccactt gctgccgtcg ccgagaagcg cctgaaaccg cggtgtatct tccaccgtcg atatgacgag cacatcgcgt atgcccgcta ccatcagcgt gcaaagcggg tagtaaatca tcggcttgtc gtagacggga agtagctgct tcgaaaccga atgcgtgacc ggatacagac gcgtacccga cccgccagcg aggatgattc ctttacgagc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMA RKGIILAGGS GTRLYPVTHS VSKQLLPVYD KPMIYYPLCT LMVAGIRDVL VISTVEDTPR FQALLGDGSK WGINLQYALQ PSPDGLAQAF IIGRSFVGND NSALVLGDNI FYGHELVKQL AHANDRDDGA TVFAYHVNDP ERYGVVEFDP EFKALSIEEK PAKPRSSYAV TGLYFYDRQV CDIAADIKPS ARGELEITDV NVHYLRRQQL HVEIMGRGYA WLDTGTHDSL IDASTFIATL QKRQGMMVAC PEEIAFRMQW IDGDQLQALA RPLSKSAYGR YLTDIAQDRV AWPTREATVE AEPQHIGSGR IRRVS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctc gtaaaggaat catcctcgct ggcgggtcgg gtacgcgtct gtatccggtc acgcattcgg tttcgaagca gctacttccc gtctacgaca agccgatgat ttactacccg ctttgcacgc tgatggtagc gggcatacgc gatgtgctcg tcatatcgac ggtggaagat acaccgcggt ttcaggcgct tctcggcgac ggcagcaagt ggggcatcaa tctgcaatat gcgctgcagc catcgccgga cggactggcc caggcgttca tcatcggccg ctcgttcgtc ggaaacgaca actctgcact ggttcttggg gacaatatct tttacgggca cgaactggtg aagcagctgg cgcacgcaaa cgaccgcgac gacggtgcga cggtatttgc ctatcatgtg aacgatccgg aacggtatgg cgttgtcgaa ttcgacccgg aattcaaggc gctttcaatc gaagagaagc cggcgaagcc gcgttcgagc tacgccgtta ccgggctcta cttctacgac cgacaggtct gcgacattgc ggcggacatc aagccttcgg cacgcggcga gctcgaaatc acggacgtca acgtacatta tctgcggcgt cagcaattgc atgtcgagat catgggacgg ggttacgcgt ggctggatac gggcacccat gactcgttga tcgacgcgtc cacctttatc gcaacgcttc agaagcggca gggcatgatg gtcgcttgcc cggaagaaat cgcgtttcgc atgcaatgga tcgacggcga ccagttgcag gccctcgccc gaccgctgtc gaagtcggct tacggccggt acctgacgga cattgcgcag gaccgggtcg catggccgac cagggaggcc actgtagagg cagaacctca acatattggt agcggccgca ttcggcgcgt ttcttgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat