BuphA.00118.b

UTP-glucose-1-phosphate uridylyltransferase

CENTER ID: BuphA.00118.b
ORGANISM: Burkholderia phymatum STM815
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuphA.00118.b.B2.GE40198 Mature protein( BuphA.00118.b ) 27 300
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|391038.7.peg.1141
RefSeq: YP_001857312.1
UniProt: B2JGT1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHTMLKVTKA VFPVAGLGTR FLPATKASPK EMLPIVDKPL IQYAVEEAIA AGITEMIFVT GRSKRAIEDH FDKSYEIEAE LEARGKEQLL DLVRSIKPSN VDCFYVRQAE ALGLGHAVLC AEKLVGGSPF AVILADDLLH SEKPVMKQLV DTFNHYHSSV VGVETIAREA SRSYGVVDGK EWEEDVIKLS GIVEKPAPDK APSNLGVVGR YVLMPTIFKH IRALKPGAGG ELQLTDALQS LLTEEQVLAY RYFGTRFDCG SKLGYLKATV EFALRHPEVR ADFEDFLQSY LPLHLTAAAA
NT Sequence
ATGCATACCA TGCTGAAAGT CACCAAAGCC GTCTTTCCTG TTGCGGGTCT CGGCACACGT TTTCTGCCTG CCACGAAGGC GAGCCCGAAG GAAATGCTGC CCATCGTCGA CAAGCCGCTG ATCCAGTACG CAGTCGAAGA AGCAATCGCA GCGGGCATCA CCGAAATGAT CTTCGTCACG GGCCGCAGCA AGCGGGCCAT CGAAGATCAC TTCGACAAGT CCTATGAGAT CGAAGCCGAA CTCGAAGCAC GCGGCAAGGA ACAGTTGCTG GATCTCGTGC GCAGCATCAA GCCGTCGAAC GTCGACTGCT TCTATGTGCG CCAGGCGGAA GCGCTGGGTC TCGGTCATGC CGTGCTGTGC GCGGAAAAAC TGGTCGGCGG CAGCCCGTTC GCCGTGATTC TCGCGGACGA CCTGCTGCAC AGCGAAAAGC CCGTGATGAA GCAGCTCGTC GACACGTTCA ATCACTATCA CAGCTCGGTG GTCGGCGTCG AAACGATCGC GCGCGAAGCG AGCCGTTCGT ACGGCGTCGT CGACGGCAAG GAGTGGGAAG AGGACGTGAT CAAGCTGTCC GGCATCGTCG AAAAGCCGGC GCCCGACAAG GCCCCGTCGA ATCTCGGCGT GGTTGGCCGC TACGTGCTGA TGCCGACCAT CTTCAAGCAC ATCCGCGCGC TGAAGCCGGG CGCAGGCGGC GAACTGCAAC TGACGGATGC GCTGCAATCG CTGCTGACGG AAGAGCAGGT GCTCGCCTAT CGCTACTTCG GTACGCGTTT CGATTGCGGC AGCAAGCTGG GCTACCTGAA AGCGACGGTG GAATTCGCGC TGCGTCATCC CGAAGTGCGC GCGGATTTCG AAGACTTCCT GCAAAGCTAT CTGCCGCTGC ATCTGACGGC AGCGGCTGCC
Details for BuphA.00118.b.B2.GE40198
HARVESTED ON: 5/9/2016
SEQUENCED ON: 5/23/2016
EXPECTED MW: 31kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMA SPKEMLPIVD KPLIQYAVEE AIAAGITEMI FVTGRSKRAI EDHFDKSYEI EAELEARGKE QLLDLVRSIK PSNVDCFYVR QAEALGLGHA VLCAEKLVGG SPFAVILADD LLHSEKPVMK QLVDTFNHYH SSVVGVETIA REASRSYGVV DGKEWEEDVI KLSGIVEKPA PDKAPSNLGV VGRYVLMPTI FKHIRALKPG AGGELQLTDA LQSLLTEEQV LAYRYFGTRF DCGSKLGYLK ATVEFALRHP EVRADFEDFL QSYLXXHLTA AAA
Validated NT Sequence
atcttactca cttaggcagc cgctgccgtc agatgnnnnn ncagatagct ttgcaggaag tcttcgaaat ccgcgcgcac ttcgggatga cgcagcgcga attccaccgt cgctttcagg tagcccagct tgctgccgca atcgaaacgc gtaccgaagt agcgataggc gagcacctgc tcttccgtca gcagcgattg cagcgcatcc gtcagttgca gttcgccgcc tgcgcccggc ttcagcgcgc ggatgtgctt gaagatggtc ggcatcagca cgtagcggcc aaccacgccg agattcgacg gggccttgtc gggcgccggc ttttcgacga tgccggacag cttgatcacg tcctcttccc actccttgcc gtcgacgacg ccgtacgaac ggctcgcttc gcgcgcgatc gtttcgacgc cgaccaccga gctgtgatag tgattgaacg tgtcgacgag ctgcttcatc acgggctttt cgctgtgcag caggtcgtcc gcgagaatca cggcgaacgg gctgccgccg accagttttt ccgcgcacag cacggcatga ccgagaccca gcgcttccgc ctggcgcaca tagaagcagt cgacgttcga cggcttgatg ctgcgcacga gatccagcaa ctgttccttg ccgcgtgctt cgagttcggc ttcgatctca taggacttgt cgaagtgatc ttcgatggcc cgcttgctgc ggcccgtgac gaagatcatt tcggtgatgc ccgctgcgat tgcttcttcg actgcgtact ggatcagcgg cttgtcgacg atgggcagca tttccttcgg gctcgccata tggtggtggt ggtggtgagc catggtat
Expected Protein Sequence
MAHHHHHHAS PKEMLPIVDK PLIQYAVEEA IAAGITEMIF VTGRSKRAIE DHFDKSYEIE AELEARGKEQ LLDLVRSIKP SNVDCFYVRQ AEALGLGHAV LCAEKLVGGS PFAVILADDL LHSEKPVMKQ LVDTFNHYHS SVVGVETIAR EASRSYGVVD GKEWEEDVIK LSGIVEKPAP DKAPSNLGVV GRYVLMPTIF KHIRALKPGA GGELQLTDAL QSLLTEEQVL AYRYFGTRFD CGSKLGYLKA TVEFALRHPE VRADFEDFLQ SYLPLHLTAA AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgcgagcc cgaaggaaat gctgcccatc gtcgacaagc cgctgatcca gtacgcagtc gaagaagcaa tcgcagcggg catcaccgaa atgatcttcg tcacgggccg cagcaagcgg gccatcgaag atcacttcga caagtcctat gagatcgaag ccgaactcga agcacgcggc aaggaacagt tgctggatct cgtgcgcagc atcaagccgt cgaacgtcga ctgcttctat gtgcgccagg cggaagcgct gggtctcggt catgccgtgc tgtgcgcgga aaaactggtc ggcggcagcc cgttcgccgt gattctcgcg gacgacctgc tgcacagcga aaagcccgtg atgaagcagc tcgtcgacac gttcaatcac tatcacagct cggtggtcgg cgtcgaaacg atcgcgcgcg aagcgagccg ttcgtacggc gtcgtcgacg gcaaggagtg ggaagaggac gtgatcaagc tgtccggcat cgtcgaaaag ccggcgcccg acaaggcccc gtcgaatctc ggcgtggttg gccgctacgt gctgatgccg accatcttca agcacatccg cgcgctgaag ccgggcgcag gcggcgaact gcaactgacg gatgcgctgc aatcgctgct gacggaagag caggtgctcg cctatcgcta cttcggtacg cgtttcgatt gcggcagcaa gctgggctac ctgaaagcga cggtggaatt cgcgctgcgt catcccgaag tgcgcgcgga tttcgaagac ttcctgcaaa gctatctgcc gctgcatctg acggcagcgg ctgcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat